alexa A Comparison of the Effects of Different Equipment used for Venipuncture to Aid in Promoting More Effective Simulation Education | OMICS International
ISSN: 2155-9864
Journal of Blood Disorders & Transfusion

Like us on:

Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

A Comparison of the Effects of Different Equipment used for Venipuncture to Aid in Promoting More Effective Simulation Education

Chieko Fujii1*, Hiroyuki Ishii2 and Atsuo Takanishi3

1Faculty of Nursing and Medical Care, Keio University, Endo4411 Fujisawa City, Kanagawa, 252-0883, Japan

2Research Institute for Science and Engineering, Waseda University, Tokyo, Japan

3Faculty of Science and Engineering, Waseda University, Tokyo, Japan

*Corresponding Author:
Chieko Fujii
Faculty of Nursing and Medical Care
Keio University Endo4411 Fujisawa City
Kanagawa, 252-0883, Japan
Tel: +81 466-49-6218
E-mail: [email protected]

Received date: June 03, 2014; Accepted date: Auguste 31, 2014; Published date: September 05, 2014

Citation: Fujii C, Ishii H, Takanishi A (2014) A Comparison of the Effects of Different Equipment used for Venipuncture to Aid in Promoting More Effective Simulation Education. J Blood Disord Transfus 5:228. doi:10.4172/2155-9864.1000228

Copyright: © 2014 Fujii C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Journal of Blood Disorders & Transfusion

Abstract

Patient safety during venipuncture is of utmost importance. In Japan, good venipuncture practices are developed in nursing schools and hospitals by the use of educational simulators. This study examined techniques used by experienced nurses to educate nursing students and nurses on venipuncture techniques. Forty-four nurses completed a questionnaire and were videotaped during venipuncture performed on a model of a simulated arm that we had developed. Using software to analyze motion and movement along the X-Y coordinates, we measured puncture angles and distance travelled by the needle using a 20 ml syringe, other syringe sizes, and vacuum tubes. Needle and hand movement were also examined. Venipuncture requires a point of a vein to be identified and insertion of a needle into that puncture site. Simulation training may assist to consciously avoid unnecessary movements caused by hand rotation and also emphasizes the importance of learning the basic movements for needle insertion. It is important that educational methods and evaluation are linked and developed using an appropriate simulator of the venipuncture technique.

Keywords

Motion analysis; Needle movement; Nursing practice; Simulation; Skill evaluation

Introduction

Prior to performing venipuncture in clinical practice a controlled environment needs to be established under the direction and supervision of clinical experts [1]. Recently, nursing students have numerous opportunities to practice their skill without causing harm to patients [2], with the prelicense nursing curriculum examining patient safety and quality concerns [3]. Simulated learning in a clinical skills center has become popular in undergraduate nursing education. Simulation provides a positive learning experience in which students can refine their patient management skills [4].

Since 2002, the Health Policy Bureau of the Ministry of Health, Labour and Welfare in Japan has regarded Intravenous injections as part of the role of nurses. Venipuncture was included with these injections as some nurses were also responsible for collection of blood samples. The safety of patients was also regarded as important.

Nursing students may not get the opportunity to perform a venipuncture on a patient, with a simulator providing the opportunity to practice the technique before carrying it out in the hospital environment. In hospitals offering educational programs, retraining may also act as an added incentive for nurses considering employment. Venipuncture techniques are also taught using simulators in both nursing schools and hospitals.

Many academic and healthcare settings currently use simulation to allow students to exercise their knowledge and skills in a virtual clinical setting [5]. Developing clinical skills, first through simulation and then through patient clinical care, is extremely valuable [6]. Highfidelity patient simulation is becoming increasingly integral to nursing education [7]. Training programs for intravenous catheter placement generally use plastic arm models, although the lack of anatomic variability and variable design of these models can diminish the educational experience for students [8]. However, numerous forearm models are constructed so that they can be deployed in limited spaces and are therefore easy to use for simultaneous group training sessions. To achieve this objective, simulators must be used effectively. After choosing the most suitable simulator, it is important to determine how to establish the best learning environment and also how we evaluate venipuncture technique. Further research is required to assess the effectiveness of patient simulation in achieving learning objectives and clinical competence [9]. It has also been suggested that the rigor and quality of this research should be improved [10].

For venipuncture it is necessary to consider the angles the skin is punctured and the needle enters the vein. Still image showed that after the needle tip touched the skin, 77% of participants raised or lowered the needle relative to a straight trajectory [11]. Although textbook and instructors of venipuncture generally cite puncture angles of approximately 20° or less, published empirical evidence is lacking. To reduce the risk of nerve damage, it is necessary to understand how different-sized equipment affects the length of needle entry. It appears that awareness and knowledge on potential differences in puncture angle and inward movement of the needle tip may lead to safer practices, even when different types of equipment are used.

It is necessary to explain the venipuncture technique of experienced nurses using a simulator and whether there are differences in the devices used and how the technique is evaluated. This study aimed to examine techniques used by nurses who regularly perform venipuncture, so that effective methods can be developed to educate nursing students and nurses who are still learning venipuncture techniques.

Methods

Study population

The study was performed in a metropolitan hospital in Japan over a three-day period in August 2010. Forty-four nurses who were able to spend 30 min away from work each day for 3 days were enrolled in the study. The 44 participants had a mean age (± standard deviation) of 36.6 ± 9.4 years and 12.0 ± 8.9 years of nursing practice. Thirty-five participants (79.5%) had performed venipunctures during the first year of their employment.

Ethical approval was obtained from the ethics committee of the Graduate School of Health Management, Keio University. The participants were provided with an explanation of the study objectives and were informed they would not be penalized for withdrawing from the study.

Data collection

The Waseda Kyotokagaku Skin No.2 Refined II; WKS-2RII model [12] was used to imitate surgical procedures and provide quantitative information on the learning progress of the trainees. We modified the model to assess venipuncture techniques so that it represented a model for puncturing the skin. The vascular model consisted of simulated skin and veins placed on a urethane base and provided a replicate of the cubital region. The simulated vein had an external diameter of 3 mm and internal diameter of 2 mm, and was located between models of fat (8-mm-thick urethane foam) and skin (2.0-2.5-mm-thick silicone). The nurses confirmed that the vein could be palpated through the skin surface.

Some images were unusable due to excess hand movement and therefore the number of venipunctures was different between nurses. The number that we were able to analyze as a picture was as follows: a total of 191 venipunctures were performed by 44 nurses using a 20 ml syringe, with 32 performing 5 punctures and 4 performing 4 punctures. Unusable moving images were excluded from the analyses. For vacuum tube sampling, 30 nurses performed 5 punctures, 2 nurses performed 2-4 punctures each, and 3 nurses performed 3 punctures, making a total of 171 punctures.

The nurses were requested to perform each technique using both a 10 ml and 2.5 ml syringe. The raw data of film footage of 39 subjects were analyzed.

Venipuncture device

The nurse was required to choose the size of the syringe according to the volume of blood required for the clinical situation. The following syringes and injection needles were therefore used: 20 ml, SS-20ESZ, diameter (Φ) 21.7 mm; 10 ml SS-10ESZ, Φ17.2 mm or 2.5 ml SS-02SZ, Φ10.0 mm; 21-gauge SB needle (NN-2138S); Terumo Corporation, Tokyo, Japan). A vacuum tube holder (XX-VP010HD01 inside diameter 20mm) and straight needles (21 gauge, MN-HD2138MS) were used for the evacuation tube collection system. These devices are used commonly in Japan, and are currently used at the facilities where the surveys were carried out.

Data processing procedure and analysis

Photographing

A Digital HD Video camera recorder (HDR-HC9, Sony, Japan) mounted on a tripod and hi-vision mini-DV tapes were used for recording the procedures. The maximum telephoto zoom (10 times) allowed a distance of 50 cm from the camera lens to the puncture point.

Preparation of the AVI file

The video recordings were captured using video editing software EDIUS Pro 5 (Grass Valley, K.K., Japan). The frame rate of the AVI file was 59.94 frames/ second. To facilitate the acquisition process, we then converted these files into 24-bit true color using a video capture of 720 x 480 pixels whilst matching them on a monitor.

Analysis of images

WINanalyze software (Mikromak GmbH, Germany, and Agency of Bertec Japan) tracks objects without markers, and assists in analyzing movements on video recordings. This software is suitable for Windows AVI. A solid-state drive (SSD)-correlation tracking algorithm was activated during acquisition to adapt the quality of the image sequence and account for differences in color. Coordinate points for X and Y in each frame were created by WINanalyze. An advantage of the software was that the needle could be kept aseptic.

Data analysis

Data obtained from the X and Y coordinates were saved in Excel (Microsoft Corporation, USA, Japanese edition) and analyzed using SPSS software (SPSS 19.0 for Windows; IBM, Japan). Unpaired t-tests were used to examine intergroup differences (e.g., 20 ml syringe vs. (1) vacuum tube (2) 10 ml syringe and (3) 2.5 ml syringe). Student’s t-test was applied when homoscedasticity was demonstrated, with Welch’s t-test being used in these cases. P values <0.05 were considered statistically significant

Tracking Points

Figure 1 shows the points for easiest tracking.

blood-disorders-transfusion-Objects-all-frames

Figure 1: Image sequence function and connection points that result in the easiest tracking
1) Connections, 2) Objects in all frames, 3) Objects in a frame, 4) X- and Y-coordinates on the vertical axis and time on the horizontal axis Point A: The needle makes contact with the model; designated as time (a). Point B: Needle movement; the welded part of the needle and the needle hub. Point C: Hand movement; the metatarsophalangeal joint of the first or second finger. Time (b): Cessation of needle movement.

Point A: Contact between the needle and model. This frame was designated as Time (a).

Point B: Needle movement with the welded part and hub of the needle being cream color for easy recognition.

Point C: Hand movement. Metacarpophalangeal joint (MP joint) of the first or second fingers.

Time (b) was the frame showing cessation of needle movement.

Observation Endpoints

Calculation of time

The number of frames from time (b) to time (a) in the AVI file was counted using a frame rate of 59.94 frames/second, with the total being reduced by 1.

Calculation of distance between multiple punctures

We used the formula of the trigonometric function using two coordinates. The difference between puncture points during multiple punctures was also determined. Multiple punctures were performed using either a 20 ml syringe or vacuum tube. The distances between puncture Point A and the next puncture point, and the final and first puncture points were calculated for each nurse. The length of the needle was kept constant. However, the distance in each picture was not always the same, with the calculation bias corrected using needle length.

Calculation of angle

The angle of points B and C relative to point A was calculated using a formula that included the two coordinates.

X axis and Y axis movement: This study measured changes in angle visually on the WINanalyze screen which displayed a graph using a 20 ml syringe. We also carried out abstraction using symbolic description. The movements were analyzed after assigning indicators, namely, I, V, Λ (Greek character lambda), N, ? (Russian character “ee”), M, and W, that corresponded to the shapes formed by the movements.

Results

Differences using the equipment

Table 1 shows the differences in duration of blood sampling using a 20 ml syringe (2.5 ± 1.0 seconds), vacuum tube, and 2.5 ml and 10 ml syringes. Once the needle tip made contact with the model, the time from puncture to cessation of movement was longest using the 10 ml syringe (2.9 ± 1.3 seconds; P = 0.038).

  Vacuum tube holder              Syringe and injection needle Syringe(20ml)
Vs.
n=171 Syringe(20ml) Syringe(20ml) Syringe(20ml) Vacuum Tube holder Syringe (10ml) Syringe (2.5ml)
n=191 n=39 n=39
Mean   SD Mean SD Mean SD Mean SD P-value
 Time from puncture to when movement stopped
Mean (seconds) 2.8
1.3
2.5
1.0
2.9
1.3
2.8
1.0

0.051†

0.038

0.091
Maximum (seconds) 8.8 7.3 8.0 5.5
Minimum (seconds) 0.0   0.7   0.0   1.3  
Distance travelled by needle
Mean (mm) 12.4
4.0
12.1
4.5
14.9
4.7
15.4
3.8

0.586

0.001

P<0.001
Maximum (mm) 21.8 24.6 24.7 24.5
Minimum (mm) 0.2 0.8 0.4 9.3
Skin puncture Puncture angle

a)
Mean (°)  17.4
5.1
14.2
6.3
13.9
6.1
15.9
5.6

P<0.001

0.782

0.137
Maximum (°)  44.7 55.6 36.1 30.3
Minimum (°)  9.7 2.8 5.3 6.4

b)§
Mean (°)  14.4
4.9
8.6
6.8
7.7
3.5
10.3
7.2

P<0.001

0.376

0.156
Maximum (°)  30.8 55.3 14.4 44.9
Minimum (°)  0.2 0.3 0.4 1.0
 Angle changes (b-a) -3.0 -5.6 -6.3 -5.5  
MP joint and puncture point
a) Mean (°) 36.1 8.6 28.9 10.4
30.7

9.4

32.8

10.2

P<0.001

0.311

0.033
Maximum (°) 65.9 77.1 57.2 65.2
Minimum (°) 17.4 5.4 11.7 18.2

b)§
Mean (°) 39.5
9.1
30.0 9.6 31.5
8.4
35.4
9.9

P<0.001

0.367

0.002
Maximum (°) 63.0 76.0 48.6 69.3
Minimum (°) 18.0 6.6 3.8 18.4
 Angle changes (b-a) 3.4 1.1 0.8 2.7 0.006
Differences in puncture points                                       n=166                      n=186
Mean (°)
Maximum (°)
Minimum (°)
3.9          3.1                  5.0          4.4
8.8          7.9                 21.4         6.2
0.4          0.4                 1.1           0.4

Table 1: Differences in blood sampling using a 20 ml syringe, vacuum tube sampling, and a 10 ml or 2.5 ml syringe.

After making the puncture, the average distance travelled by the needle was 15.4 ± 3.8 mm for the 2.5 ml syringe and 14.9 ± 4.7 with the 10 ml syringe. Both distances exceeded the average distance travelled by the needle using a 20 ml syringe (12.1 ± 4.5 mm). Significant differences were observed between distances travelled by the needle using 10 ml (P=0.001) and 2.5 ml syringes (P<0.001) compared with the 20 ml syringe. The mean puncture angle was 14.2° ± 6.3° with the 20 ml syringe and 17.4° ± 5.1° with the vacuum tube. This difference was statistically significant (P<0.001). The angle between the MP joint and puncture site was significantly different between the 20 ml syringe 28.9° ± 10.4° and both the vacuum tube (36.1° ± 8.6°; P < 0.001) and 2.5 ml syringe (32.8° ± 10.2°; P=0.033). The maximum angle observed during puncture with a 20 ml syringe was 55.6°.

Assessment of angle changes at times (a) and (b) showed that compared to time (a) when the needle tip touched the model, the needle joint angle decreased at time (b) after the needle had been moved. However, the angle of the MP joint increased. To calculate differences in puncture points during multiple punctures, the study averaged the differences in positions for subjects who attempted multiple trials with the 20 ml syringe and vacuum tube. The mean difference for the 20 ml syringe was 5.0 mm, with a maximum value of 21.4 mm, while the mean difference for the vacuum tube was 3.9 mm. This demonstrated the 20 ml syringe resulted in significantly greater changes in position (P=0.006).

Example showing the movement of various needles

Figure 2 shows the 20 ml syringe technique with five puncture points grouped in close proximity. For procedures resulting in five punctures, the distance from the previous to subsequent puncture was measured using the coordinate points, and the mean distance calculated. The mean distance observed in the target film footage of closely grouped puncture points, identified by the image sequence function using objects from all frames, was 1.1 mm with the 20 ml syringe. Although the puncture points were the same, needle and MP joint movements appeared inconsistent. When making the fourth puncture, movements were observed four times in both the X- and Y-axes. However, the needle did not move in a diagonal direction, with the needle and MP joint movements differing.

blood-disorders-transfusion-performed-close-proximity

Figure 2: An example of venipuncture using a 20 ml syringe. In this case, five punctures were performed in close proximity. Needle and metatarsophalangeal joint movements were inconsistent and varied particularly during the fourth puncture.

Classification of needle movement

Table 2 shows the comparison of frequency of needle movements categorized by symbolic descriptors. Horizontal movements along the X-axis occurred up to 5 times or more, whereas vertical movements along the Y-axis entailed 1 to 4 changes in movement. At the welded part of the needle, movement in the X-axis was characterized by I, whereas movement in the Y-axis was characterized by I in 24.6%, V in 4.2%, Λ in 13.6%, N in 1.6%, and ? in 22.0% of cases. X-axis movement was I or V, and Y-axis movement, enclosed with bold lines, was I, V, Λ, N, or ? in 72.8% of cases at the welded part of the needle and 36.1% at the MP joint.

blood-disorders-transfusion-frequency-needle-movements

Table 2: Comparsion of frequency of needle movements categorized by symbolic descriptors.

Data showing needle movement in a large proportion of the venipunctures

Figure 3 shows a large proportion of needle movement due to combined movement in the X- and Y-axes. Nearly 44% of X- and Y-axis movements at the MP joint consisted of combinations of I and Λ, I and I, N and Λ, or Λ and Λ movements. At the welded part of the needle, 67.5% of movements in the X- and Y-axes entailed a combination of I and I, I and ?, I and Λ, and N and ? movements. These data were derived from photographs of movement at time (a). An Excel database was prepared of coordinate data grouped according to time, and the coordinates superposed on a photograph as needle movement.

blood-disorders-transfusion-large-proportion-needle

Figure 3: A large proportion of needle movement was due to the combined movements in the X- and Y-axes. These photographs show the large proportion of needle movement in the X–Y axis at time (a). The coordinates are superposed on a photograph as needle movement using Excel.

Discussion

Using a simulator to learn skills to ensure patient safety

Due to the limited availability of clinical sites and shorter hospital stays, patients now present with more acute and complex healthcare issues and needs [13]. Students are therefore not always able to receive appropriate clinical experience. Nevertheless, patient safety has been emphasized and questions are now being asked regarding the types of training that should be implemented before actual clinical practice. Practical clinical skills lie at the heart of nurses’ professional practice. Therefore, mastery of a fundamental clinical skill is an important component of courses leading to registration [14]. An integrated approach for training of clinical skills in undergraduate nursing students is therefore necessary [15]. The use of simulated technology addresses patient safety as students are able to refine their skills and develop competency in this environment [16].

Evaluation using the simulator

Further work is needed to strengthen the evidence base for the use of simulation models [17]. Simulation-based methods can also be implemented in rural locations to develop emergency medical skills or obstetric first-aid skills for birth attendants [18]. Numerous studies have considered how to use simulators as educational tools.

The plastic arm, however, does not convey pain response feedback by the patient during venipuncture caused by technical mistakes and failure to simulate realistic pressures and touch feedback. Despite this limitation, the students commented on the superiority of the plastic arm and its realistic features regarding arm stability and tourniquet use. Medical and nursing students enjoy and prefer one-on–one training with a faculty member using virtual reality models [8]. Simulationbased healthcare education uses well-defined learning objectives and tasks with an appropriate level of difficulty [19]. Efforts to reduce occupational health and safety risks include staff education, safer equipment, and the reduction of needle-dependent procedures [20].

In this study, we used the simulator to evaluate the venipuncture maneuvers of experienced nurses. The method these experienced nurses used was not necessarily according to the textbook. In particular there were individual differences in three main areas; differences in syringe size and needle movement, and not needling the same point in multiple punctures. It is therefore important to learn how to use different equipment and also to train puncturing the same point with the needle.

Experiment using realistic equipment

This study was the first to measure the actual angles, and showed mean puncture angle using the same model was 17° with vacuum tubes, 16° with 2.5 ml syringes, and 14° with 20 ml and 10 ml syringes. The maximum angle of 56° was observed with a 20 ml syringe.

This study estimated the distance travelled by the needle. Using a 38-mm long needle, the length of needle entering the model was greatest with the 2.5 ml syringe at 15.4 mm (40.5% of the needle), while with a 20 ml syringe, the needle travelled 12.1 mm (31.8% of the needle). Using the 20 ml syringe as reference, the 2.5 ml syringe travelled 3.3 mm further. Despite remarkable advances in technology and laboratory automation, laboratory tests still have a high degree of pre-analytical variability. 23G needles, if handled correctly, will not introduce any statistically or clinically significant errors in measurement compared with 21G needles [21].

Needle stick injuries expose healthcare providers to dangerous blood borne pathogens, including human immunodeficiency, hepatitis B, and hepatitis C viruses. When using proactively designed safety I.V. catheters, nurses often experienced difficulties with needle insertion due to the size and overall design of the device [22]. Syringes made by different companies have variable size and it is important that simulation training uses the same venipuncture device used in the clinical situation. It is therefore important that optimal techniques incorporating different venipuncture equipment are investigated.

Accurate puncture point

For the puncture, it is essential to place the needle exactly on the point that has been selected. During blood sampling, the puncture site is determined by palpating the vein on the skin surface. The needle is then inserted without placing any mark on the chosen site. When blood was drawn in this study using a 20 ml syringe, the greatest difference between 5 puncture points was 21 mm, with a mean difference of 5 mm. This distance was larger than the diameter of the vein used in the simulation. Practicing accurate insertion into a target point is therefore necessary.

Accurate needle movement

A simulator can evaluate needle movement during venipuncture carried out by a nurse. By following MP joint movement, we showed that needle hub and hand movements were inconsistent in some cases. The study encoded the movement of the X- and Y-axes symbolic descriptors. If the X-axis was I and the Y-axis was I, this indicated that the needle moved uniformly over a fixed distance from the time the needle tip made contact until it reached the vein.

The so-called puncture described in textbooks involves making a puncture with the X-axis at I and the Y-axis at V or N, entering the vein, and then pushing the needle into the vein. However, when considering cases in which the needle moves in an intricate manner, both the hand and the needle rotate. Even when the multiple puncture points were close to each other, both needle and MP joint movements changed during puncture. The position of the needle bore did not necessarily move upward if the hand joint moved. When the needle was turned more than necessary, this had adverse effects on the puncture site.

Simulation training requires participants to be conscious of not making unnecessary movements caused by hand rotation, with the aim of ensuring the trajectory of the needle reduces stress on the puncture site.

Conclusions

This study emphasizes the importance of learning the basic movements of needle insertion at a single point and not twisting the needle or hand more than is necessary. During the initial stages until perception of the needle tip is acquired, there is no need to practice on patients, but instead use simulated arm models.

To the best of our knowledge no other study has compared differences in puncture angle and the longer distances travelled by the needle according to the size of the equipment. It is therefore necessary to account for differences in equipment when examining different venipuncture techniques.

Hand rotation may also cause rotation of the needle bore. Our results suggest that simulators may be useful for practicing needle insertion into the same puncture site and for avoiding twisting the hand more than necessary when performing venipuncture on actual patients.

Venus cannulation is one of the main procedures carried out by nurses. For the safety of patients and nurses it is important that this skill is performed in a proper and responsible way. The need for adequate education of this skill is therefore essential [23]. By using a simulator we were able to evaluate the technique using different appliances under the same conditions. A precise skill will be achieved by nurses, leading to improved patient safety. Because of establishing skill evaluation, educational relationships will also be improved.

References

Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Relevant Topics

Article Usage

  • Total views: 12389
  • [From(publication date):
    October-2014 - Aug 18, 2018]
  • Breakdown by view type
  • HTML page views : 8586
  • PDF downloads : 3803
 

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

kactakapaniyor.com

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

Taktube

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

porn sex

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

Gaziantep Escort

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

sikiş

[email protected]

1-702-714-7001Extn: 9037

instafollowers

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

mp3 indir

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

putlockers

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

seks

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version