School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China
Received Date: November 15, 2013; Accepted Date: January 27, 2014; Published Date: January 29, 2014
Citation: Sheng L, Deng L, Yan H, Zhao Y, Dong Q, et al. (2014) A Genome-Wide Analysis of the AAAP Gene Family in Maize. J Proteomics Bioinform 7:023-033. doi:10.4172/jpb.1000299
Copyright: © 2014 Sheng L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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The AAAP (amino acid/auxin permease) genes encode a large family of transporters. A few members of the AAAP family have been characterized in Arabidopsis thaliana and rice (Oryza sativa). However, little is known about AAAP genes in maize (Zea mays). Therefore, we performed a systematic bioinformatics analysis to characterize all AAAP genes in maize, which included analysis of the genome sequence, conserved protein domains, chromosomal locations, phylogenetic relationships, gene duplications, and gene expression profiles. In this study, seventy-one ZmAAAP genes were identified and named ZmAAAP01 to ZmAAAP71. The number of AAAP genes in maize is more than the number in Arabidopsis thaliana (43) and in rice (58). We found a higher percentage of AAAP gene duplications in the maize genome, which contributed to the expansion of the AAAP gene family. Furthermore, segmental duplications played a major role in the AAAP gene expansion in maize. The AAAP genes are unevenly distributed on the 10 maize chromosomes, and 31 of them are distributed within 12 clusters on segmental chromosomes. Both the EST analysis and transcriptome data indicated that most ZmAAAP genes exhibited abundant expression patterns, suggesting diverse and novel functions of AAAP gene family in maize. The results presented here provide useful information for further functional analysis of the AAAP gene family in maize.
Maize; AAAP gene family; Phylogenetic analysis; Gene duplication; Chromosomal location; EST analysis
Amino acids serve as primary sources of organic nitrogen for the growth of many eukaryotic cells . Additionally, they also serve as neurotransmitters and hormones, allowing communication between cells and tissues within multicellular organisms [2,3]. Some amino acids even allow adaptation to environmental change, especially to those causing organismal stress . These functions require the presence of transport systems that catalyze uptake into, and release from specialized cell types .
Amino acid transporters are the principal mediators of organic N distribution, and are important regulators of resource allocation in plants. In recent years, a large number of gene families of amino acid transporters have been identified, such as the amino acid/polyamine/ choline (APC) family [6,7], the amino acid transporter gene family (AAT) in rice , the lysine-histidine-like transporter family (LHT) in Arabidopsis  etc. These genes are widely distributed in eukaryotic organisms (ranging from yeast and plants to insects and mammals), and many of these genes have important biological functions in eukaryotes.
The amino acid/auxin permease (AAAP) family is one of the largest families of amino acid transporters identified so far, with members found in virtually all eukaryotic organisms [1,2,10]. The AAAP family belongs to the “Electrochemical Potential Driven Transporters” class, which includes hundreds of proteins from plants, animals, yeast, and other fungi. AUX1/LAX proteins [3,11], belong to the Amino Acid/Auxin Permease (AAAP) family are proton-gradient-driven secondary transporters, where auxin influx is mediated as cotransport with proton(s). Individual proteins in AAAP family are amino acid permeases that transport auxin (indole-3-acetic acid), a single amino acid, or multiple amino acids. Some of these permeases exhibit very broad specificities transporting all twenty amino acids naturally found in proteins. Some also transport D-amino acids. Apparent differences in substrate specificity influenced by structure and net charge are observed within these carriers and may be expected also among AUX1/ LAX proteins . Genes in the AAAP family encode transporters with a variety of functions involved in the developmental and physiological processes in plants. For example, AtAAP1 was highly expressed in the cotyledons and the endosperm and regulated the import of amino acid into root cells or developing embryo [13-15]. OsAAP8 and OsAAP15 might participate in the uptake and long-distance transport of amino acid .
Maize (Zea mays) is an important cereal crop that has also become a model species for the study of genetics, evolution, and other basic biological processes. The availability of maize genome sequence has provided an excellent opportunity for whole-genome annotation, classification, and comparative genomics research . Although the roles of many AAAP genes were revealed in extensive studies of Arabidopsis thaliana and rice, none of the ZmAAAP genes has been functionally characterized. Therefore, there is an urgent need for a thorough bioinformatics analysis of the ZmAAAP gene family. In this work, an attempt was made to find out all AAAP genes existed in maize through a systematic EST and genomic DNA sequence data mining, resulting in the identification of 71 AAAP genes. Then the complete survey of genomic organization, chromosomal location and sequence homology of all ZmAAAP genes were performed, as well as the classification of all ZmAAAP genes and the phylogenetic tree based on 71 ZmAAAP protein sequences, 58 OsAAAP protein sequences and 43 AtAAP protein sequences. In addition, the complete duplication analysis and predicted expression patterns of ZmAAAP genes will be helpful for future investigations into the biological functions of individual ZmAAAP genes as well as the evolution of AAAP genes in different plant species.
Identification of the maize AAAP gene family and analysis of conserved domains
Maize genome sequences were downloaded from http://www.maizesequence.org/. The sequences of AAAP genes from Arabidopsis thaliana were downloaded from The Arabidopsis Information Resource (TAIR) website (http://www.arabidopsis.org/). Initially, members of the AAAP family from Arabidopsis thaliana were used as query sequences and submitted to the PFAM database (http://pfam.janelia.org/search/sequence) to determine conserved domain motifs. The Hidden Markov Model (HMM) profile of AAAP domains from the Pfam database was then used to identify all possible AAAP genes in the maize genome using the BlastP program (P value = 0.001). Second, the Pfam database was used to determine if each of the predicted ZmAAAP gene was a member of the AAAP family. To avoid identification of overlapping genes, all of the identified ZmAAAP genes were aligned using Clustal W  as implemented in MEGA v 4.0 . On the basis of these searches, we were able to identify all members of the AAAP family in maize by using currently available genome databases.
Phylogenetic analysis of maize AAAP genes
The complete protein sequences of maize AAAP genes were merged, and multiple-sequence alignments were performed with Clustal X (version 1.83) . In order to better understand the phylogenetic relationships and group the ZmAAAP genes, a phylogenetic tree for all complete ZmAAAP protein sequences was constructed with the neighbor-joining (NJ) method using MEGA v4.0 . Default parameters were used in the neighbor-joining (NJ) method. The same methods can be applied to analyze the evolutionary relationships between the AAAP proteins in maize, rice and Arabidopsis thaliana. The protein sequences of AAAP genes from rice and Arabidopsis thaliana were downloaded from the Phytozome website (http://www.phytozome.net/rice.php; http://www.phytozome.net/arabidopsis.php), and a phylogenetic tree based on 71 ZmAAAP protein sequences, 58 OsAAAP protein sequences and 43 AtAAP protein sequences was then constructed from the protein sequence alignment.
Sequence analysis and duplication patterns of ZmAAAP genes
On the basis of the phylogenetic analysis, the conserved motifs in the ZmAAAP proteins were characterized using MEME (http://meme.sdsc.edu/meme/meme.html)  to identify structural divergence between different subgroups of the AAAP gene family. The number of amino acids (length), molecular weight (MW), isoelectric point (pI) and length of the open reading frame (ORF) for each ZmAAAP gene were obtained from Expasy (http://www.expasy.ch/tools). Gene duplication events for the AAAP genes in maize B73 were also investigated. All of the confirmed AAAP genes from the maize genome were aligned using ClustalW and analyzed using MEGA v4.0 on the basis of the phylogenetic tree.
Mapping ZmAAAPs on the maize chromosomes
Each non-overlapping ZmAAAP gene sequence was used as a query against the maize genome sequence (http://maizesequence.org/blast), analyzed with the TBlastN program, and positioned on the 10 maize chromosomes. ZmAAAP gene names are assigned according to their position from the top to the bottom of maize chromosomes 1–10. The chromosome map showing the physical location of all ZmAAAP genes was generated with Genome Pixelizer software (http://www.niblrrs. ucdavis.edu/GenomePixelizer/GenomePixelizer_Welcome.html).
EST expression profile analysis of the ZmAAAP gene family
The analysis of ZmAAAP gene expression profiles was accomplished by searching the maize dbEST database (http://www.ncbi.nlm.nih.gov/dbEST/) and finding expression information provided at the Web sites. Maize gene expression data were first obtained through blast searches against the maize dbEST database downloaded from NCBI conducted with the DNATOOLS Blast program.
Search parameters were as follows: maximum identity >95%, length >200 bp, and E value <10-10. In addition to the maize EST database, maize gene expression data were also extracted from the Maize Assembled Genomic Island (MAGI) (http://magi.plantgenomics.iastate.edu/) and the Plant Genomic Database (Plant GBD) (http://www.plantgdb.org/) and included ESTs, cDNAs, and PUTs (Plant- GDB unique transcripts).
Constructing three-dimensional models of ZmAAAP homologous genes
In order to make primary analysis on structures of ZmAAAP homologous genes, we selected ZmAAAP40 and its three homologous genes as an example to construct three-dimensional models. First, the protein sequences of four homologous genes were submitted to the SWISS-MODEL (http://swissmodel.expasy.org/), respectively.
And the homologous protein sequences were selected for constructing three-dimensional models. Second, the selected protein sequences were submitted to the SWISS-MODEL (http://swissmodel.expasy.org/) again for homology modeling analysis. Then the pdb files of four ZmAAAP proteins were downloaded. At last, accurate structures were built using Swiss-Pdb Viewer .
Identification of AAAP genes in the maize genome
We used BLASTP searches based on the conserved AAAP domain HMM profile to identify the AAAP genes present in the maize genome. Seventy-one potential AAAP protein sequences were predicted with an E value threshold of 0.001 and identified as AAAP genes using the BLAST program from the Pfam database. The 71 AAAP genes were then designated ZmAAAP01 to ZmAAAP71. Descriptive information for the maize AAAP genes included the sequence ID, amino acid length, predicted MW, predicted PI, number of ZmAAAP motifs, and the physical locations of the genes on the chromosomes. The predicted PIs for seventy-one ZmAAAP gene products are below 11.0, and most of them are about 9.0. Only two gene products have PIs below 5.0. The predicted lengths of the ZmAAAP ORFs ranged from 396 bp (ZmAAAP19) to 3807 bp (ZmAAAP67) and the predicted molecular weights ranged from 13.96 kDa (ZmAAAP19) to 140.21 kDa (ZmAAAP68). All of this information is listed in Table 1.
|Gene name||Sequence ID||Chr||ORF length (bp)||Length (aa)||MW (kDa)||PI|
ORF=open reading frame; MW=molecular weight; PI=isoelectric point
Table 1: Characteristics of the predicted AAAP proteins in maize.
Phylogenetic analysis of ZmAAAP genes
To examine the phylogenetic relationships of the AAAP genes and analyze the evolution of each subfamily, a phylogenetic tree of all full-length maize AAAP protein sequences was generated by the neighbor-joining method using the MEGA program (Figure 1). The phylogenetic tree showed that the 71 ZmAAAP genes are distributed into 30 sister groups in eight major clades, with a common ancestor and no additional descendants.
In order to better investigate the phylogenetic relationships between the same gene families in different species, we compared maize with rice and Arabidopsis thaliana. The phylogenetic tree derived from 71 ZmAAAP, 58 OsAAAP and 43 AtAAP sequences were aligned and constructed (Figure 2). The phylogenetic tree showed that members of AAAP gene family in three species share a high level of protein sequence homology. Moreover, the major clades of maize and rice are highly unified.
Analysis of conserved motifs and classification of the maize AAAP gene family
The results of the Pfam analysis showed that 71 putative ZmAAAP protein sequences contained a typical Aa trans domain. We then aligned the amino acid sequences of ZmAAAP proteins using Clustal X, and the ZmAAAP protein conserved motifs were defined by submitting their full-length amino acid sequences to MEME . Subsequently, 20 motif sequences were identified and shown in Table 2. Besides, the distributions of these motifs in ZmAAAP genes were shown in Figure 3. Analysis of the conserved motifs indicated that 69 of 71 ZmAAAP proteins contain motif 5, 68 of 71 ZmAAAP proteins contain motif 1, and 68 contain motif 4. Conserved motifs can provide evidence for classification of the maize AAAP gene family. It is possible that proteins within a subgroup that share identical motifs are likely to possess similar functions, and this could be helpful in predicting the function of these proteins.
|MOTIF||WIDTH||BEST POSSIBLE MATCH|
Table 2: Twenty putative conserved motifs were identified in the maize AAAP family using MEME search tool.
Figure 3: Twenty putative conserved motifs were identified in the maize AAAP proteins using the MEME search tool. Different motifs are indicated by different colors and the gene names are indicated on the left side of the figure. The length of motif in each protein represents the actual length and motif sizes are indicated at the bottom of the figure.
In order to better analyze the phylogenetic relationships between the same gene families in different species, we submitted the maize and Arabidopsis AAAP proteins to MEME (http://meme.sdsc.edu/meme/meme.html). Twenty putative conserved motifs were identified in the maize and Arabidopsis AAAP proteins (Table 3). The distributions of these motifs in the maize and Arabidopsis AAAP proteins were shown in Figure 4. This figure has shown that the maize and Arabidopsis AAAP proteins in the same subgroups have similar distributions of motifs. The comparison indicated that some maize and Arabidopsis AAAP genes in the same subgroups are likely to have similar functions.
|Motif||Width||Best possible match|
Table 3: Twenty putative conserved motifs were identified in the maize and Arabidopsis AAAP proteins using the MEME search tool.
Figure 4: Twenty putative conserved motifs were identified in the maize and Arabidopsis AAAP proteins using the MEME search tool. Different motifs are indicated by different colors and the names of all members are shown on the left side of the figure. The markers next to the gene names represent the subgroups to which each AAAP gene belongs. The length of motif in each protein represents the actual length and motif sizes are indicated at the bottom of the figure.
The AAAP family proteins in maize were classified into eight distinct subfamilies based upon their sequence composition and phylogenetic relationship, including amino acid permeases (AAPs), lysine and histidine transporters (LHTs), proline transporters (ProTs), GABA transporters (GATs), auxin transporters (AUXs), aromatic and neutral amino acid transporters (ANTs) and amino acid transporterlike (ATL) subfamilies. ATL subfamilies consist of two phylogenetic clades that are named as ATLa and ATLb, respectively.
Chromosomal locations of the ZmAAAP genes
Based on available information (http://www.maizegenome.org/data_portal.html), the identified ZmAAAP genes were positioned on the maize chromosomes using Genome Pixelizer software. All 71 ZmAAAP genes mapped to the 10 maize chromosomes. There are thirteen AAAP genes located on chromosome 3, 12 genes on chromosome 6, nine genes on chromosome 2, and eight genes on chromosome 10. Six genes each mapped to chromosomes 4 and 9, and there are five genes each on chromosomes 1 and 5. Four AAAP genes mapped to chromosome 8, and three genes to chromosome 7 (Figure 5).
Figure 5: Genomic distribution and duplications of the AAAP genes on the 10 chromosomes of maize. The chromosome numbers are shown at the top of each chromosome (vertical black bars). The names on the left side of each chromosome correspond to the approximate location of each AAAP gene. The thick black lines on the left side of the names of the AAAP genes indicate the clusters of genes on each chromosome. The thin black lines connect the AAAP paralogs lying on duplicated chromosomal segments.
In addition, several ZmAAAP genes are clustered together on segmental chromosomes. A gene cluster is a chromosome region containing two or more genes that encode the same or similar products, and gene clusters are useful for tracing recent evolutionary history [21,22]. We found that 31 of the ZmAAAP genes are located within 12 clusters, with chromosomes 2, 3, 5, 6, 7, 8, 9 and 10 containing 1, 3, 1, 1, 1, 1, 2, and 2 gene clusters, respectively; the largest gene cluster contained six ZmAAAP genes.
Based on the distribution of AAAP genes on the maize chromosomes, we found that the duplication of AAAP genes included tandem and segmental duplication events (Figure 5). Tandem duplication events accounted for less than 10% of the total number, while segmental duplication accounted for the majority of the duplication events. The distribution of AAAP genes on the rice chromosomes has been reported. Tandem duplication and segmental duplication took the similar proportion in rice AAAP family. The result suggested that large-scale segmental and tandem duplication events played a significant role in the expansion of the rice AAAP family . Therefore, the duplications of ZmAAAP genes may play an important role in a succession of maize genomic rearrangements and expansions.
Expression patterns of the ZmAAAP gene family
Since EST data can provide valuable information for gene expression research, we examined the expression patterns of maize AAAP genes in various tissues and organs using the NCBI EST database. We divided the maize EST data into 16 tissue groups: mixed, silks, apex, husks, shoot tips, leaf, suspension culture, pedicel, root, endosperm, seedling, tassel, embryo, pollen, ear, and multiple tissues (Table 4 data included as supplementary). According to the NCBI EST expression database, 10 ZmAAAP genes are not expressed, ZmAAAP40-specific mRNA was found only in the embryo, and mRNA from the remaining ZmAAAP genes were identified in two or more tissues and organs.
Although the digital EST expression analyses provided a first glimpse of the patterns of AAAP gene expression in maize, we could not draw conclusive inferences regarding AAAP gene expression. Therefore, to gain more insight into the expression patterns of the AAAP genes, a comprehensive expression analysis was further performed by using the publicly available transcriptome data for maize. Firstly, 18 tissues representing five organs have been analyzed using RNA sequencing (RNA-Seq) . Distinct expression profiles were identified for all 71 AAAP genes and the transcriptome data were shown in Table 5 (Data included as supplementary). Then the transcriptome data for the AAAP genes were imported into R and Bioconductor (http://www.bioconductor.org/) for expression analysis. We used the heat map package to make the heat map (Figure 6). Based on the results of heat map, the majority of AAAP genes showed distinct multiple expression patterns across the 18 tissues examined. The ZmAAAP40 gene was distinctly expressed only in the embryo. The transcriptome analysis of the expression patterns for all AAAP genes was consistent with the above EST analysis (Table 4 data included as supplementary). So far, there are many literatures that have reported the expression profiles of some AAAP genes in other species, such as the microarray data of gene expression for AAAP genes in rice under various abiotic stresses , AAAP transporters were significantly upregulated by FA treatment , Combining these results to our transcriptome analysis, we could find that the AAAP genes showed multiple expression patterns in many species.
Figure 6: Expression profiles of maize AAAP genes. The heat map was generated by hierarchical clustering using heat map package. The expression data were gene-wise normalized and hierarchically clustered with average linkage. The color scale in the top right corner represents the relative gene expression levels: red, yellow and blue indicate high, medium and low levels of gene expression, respectively.
We selected ZmAAAP40 and its three homologous genes as an example to construct three-dimensional models (Figure 7). By comparing their structures, we have found that homologous genes in the same subfamily have different features. The protein structure of ZmAAAP40 only contains helix and coil, as well as the protein structure of ZmAAAP04. However, the protein structures of ZmAAAP42 and ZmAAAP44 not only contain helix and coil, but also contain strands. Although ZmAAAP04 has the similar protein structure with ZmAAAP40, their expression profiles are of great differences. In addition, ZmAAAP04 and ZmAAAP44 were duplicated genes, but their protein structures were of great differences.
Duplication Contributed to Functional Divergence of ZmAAAP gene Family
When gene duplication occurs, expression patterns and original functions of these genes may be retained . Consistent with that, the comparison analysis for expression pattern of paralogous ZmAAAP genes involved in duplication indicates that ZmAAAP08 and ZmAAAP71, and ZmAAAP46 and ZmAAAP59, and ZmAAAP43and ZmAAAP68, and ZmAAAP02 and ZmAAAP32, and ZmAAAP05 and ZmAAAP16, localized on segmental duplication, exhibited similar expression patterns in development stages. However, it was well known that a great degree of expression and functional divergence might be present between two duplicated genes due to the intense selection pressure and the need for the diversification . Most duplicated ZmAAAP genes exhibit distinct expression patterns, such as ZmAAAP04 and ZmAAAP44, ZmAAAP28 and ZmAAAP39, ZmAAAP24 and ZmAAAP50, ZmAAAP21 and ZmAAAP54, etc. These results suggested that chromosomal duplication events not only facilitated the expansion of the ZmAAAP gene family, and also leaded to expression divergences between duplicate genes, further contributing to the establishment of gene functional diversity during their evolution.
Numerous trans-membrane amino acid transporters have been characterized physiologically and genetically [10,26]. The amino acid/ auxin permease (AAAP) proteins constitute a major eukaryoticspecific superfamily of amino acid transport proteins. These are secondary carrier proteins and their activity requires an independently established electrochemical gradient [10,26]. As an important gene family, the eukaryotic-specific amino acid/auxin permease (AAAP) family plays an important role in various aspects of plant growth and development. Significant progress has been made toward the identification and characterization of AAAP gene family in model plants, but little attention has been paid to AAAP gene family in maize.
In previous reports, several AAT genes in Arabidopsis were classified and functionally characterized in detail, such as genes in the AtAAP subfamily [15,27,28] and the AtAUX subfamily [3,29]. However, members of AAAP family are still unknown so far in maize, and none of them is functionally characterized. In this study, we identified 71 ZmAAAP genes which were divided into eight subfamilies based on amino acid sequence similarity, and there was obvious difference in numbers among subfamilies. The largest ZmAAP subfamily number was 24, and the smallest ZmProT and ZmGAT subfamilies were only two (Figure 1). In addition, the number of AAAP genes in maize is more than the number in Arabidopsis (43) and in rice (58). The increase in the number of ZmAAAP members contributed to the expansion of the AAAP gene family.
The phylogenetic tree describing the evolutionary relationships between AAAP genes in maize, rice and Arabidopsis thaliana showed that maize shared more homology with rice, which maybe result from that both maize and rice are monocotyledons. The result will pave the way for further bioinformatics analysis of the evolutionary relationships between the AAAP genes in maize and other plant species. Chromosomal mapping of ZmAAAP genes showed their variable distribution on 10 maize chromosomes. The most members are localized on chromosome 3 and the least members are localized on chromosome 7. Meanwhile, we found that gene duplication was common in ZmAAAP genes. Gene duplication is thought to be an important means of gene family expansion and functional diversity during evolution, which may occur through three major pathways: chromosomal segmental duplication, tandem duplication and retroposition [25,30,31]. Most gene families in maize contain gene duplications which include tandem and segmental duplications. These duplications play a major role in the expansion of gene families, such as MADS-box genes [32,33], ARF genes . In this study, our analysis on gene duplication reveals that 37 of 71(55.30%) ZmAAAP genes are duplicated genes, 32 genes (86.49%) are involved in the segmental duplication and 5 genes (13.51%) in tandem duplication. The analysis indicated that segmental duplication contributes most to the expansion of the ZmAAAP gene family, which was different with rice. In rice, the segmental and tandem duplications contribute almost equally to the expansion of the OsAAAP gene family .
Most ZmAAAP genes were found to be expressed in multiple tissues, ZmAAAP genes may have different expression patterns in different species. For example, in Arabidopsis, AtAUX1 was primarily expressed in root and promoted lateral root formation [35,36], however in rice, OsAUX1, an ortholog of AtAUX1, had high expression levels in all the organs, especially in young root and panicle . Our data showed that ZmAAAP02, an ortholog of AtAUX1 was primarily expressed in embryo and shoot apical meristem, and ZmAAAP24, an ortholog of OsAUX1 was expressed in multiple tissues, especially in leaves. By comparing expression profiles of AAAP genes in maize, rice and Arabidopsis, we found that the majority of AAAP genes in each species have multiple expression profiles. Therefore, the AAAP gene family may play an important role in growth and development of different species. In this study, we have also shown that ZmAAAP40 may be an embryo-specific gene by combining EST with transcriptome data, which provided a useful reference for cloning and further functional analysis. Meanwhile, we analyzed the three-dimensional models of ZmAAAP40 and its three homologous genes. Our analyses suggested that homologous genes may have great difference of protein structures, which might be result in different expression profiles of homologous genes. We have found that the phenomenon that homologous genes have different expression profiles also exists in other species. For example, the five pairs of paralogous genes (OsAAP11 and OsAAP16, OsGAT1 and OsGAT2, OsANT1 and OsANT2, OsANT3 and OsANT4, OsATL5 and OsATL6) have divergent expression patterns in rice .
Our study suggests that most maize AAAP genes play functional roles in more than one type of tissue. Therefore, the AAAP gene family likely plays an important role in maize developmental processes. The comparative and phylogenetic analyses of the AAAP genes in maize are first step towards a comprehensive functional characterization of this important gene family.
In conclusion, the results of this study display the analysis of the genome sequence, classification, chromosomal locations and conserved motifs of the 71 ZmAAAP members, along with their expression profiles. All 71 ZmAAAP members were divided into eight clades. The AAAP genes were unevenly distributed on 10 chromosomes, and their diverse sequence features provided potential evidence for diversifying functions. In addition, our survey showed that ZmAAAP genes exhibit various expression patterns. Our study will provide an insight into further understanding of functions of AAAP genes and their roles in maize growth and development. Overall, our findings presented here can serve as useful information for guiding future experimental work and understanding the structure-function relationship of the members in maize AAAP gene family.