alexa Advances in Clinical Diagnosis through Electrochemical Aptamer Sensors | Open Access Journals
ISSN: 1948-593X
Journal of Bioanalysis & Biomedicine
Like us on:
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Advances in Clinical Diagnosis through Electrochemical Aptamer Sensors

Pranjal Chandra*

Amity Institute of Biotechnology, Amity University Uttar Pradesh, Sector 125, Noida 201303, India

*Corresponding Author:
Pranjal Chandra
Amity Institute of Biotechnology
Amity University Uttar Pradesh, India
Tel: +91-120-4392644
Fax: +91-120-4392295
E-mail: [email protected]

Received Date: June 27, 2013; Accepted Date: July 18, 2013; Published Date: July 20, 2013

Citation: Chandra P (2013) Advances in Clinical Diagnosis through Electrochemical Aptamer Sensors. J Bioanal Biomed 5: e119. doi: 10.4172/1948-593X.1000e119

Copyright: © 2013 Chandra P. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Journal of Bioanalysis & Biomedicine

Aptamers are oligonucleotides, such as ribonucleic acid (RNA) and single-strand deoxyribonucleic acid (ssDNA) or a peptide molecule that can bind with a specific target molecule with high affinity and specificity which is due to the formation of specific three-dimensional structures. Since the discovery of aptamers, scientists have used aptamer in the fabrication of various kind biosensors such as; electrochemical, optical, fluorimetric etc. Aptamers have been considered as a biomaterial in diagnostics and also as therapeutic tool in the development of new drugs, drug delivery systems. Other advantages of aptamers are that they can be regenerated, highly stable to external factors, and does not require animal models for production [1]. Out of various kinds of clinical detection assays generally an electrochemical methods is more fascinating because it offers high sensitivity, compatibility with novel microfabrication technologies, miniaturization, and low cost. Therefore, in recent times various electrochemical aptasensors have been fabricated using several techniques such as; electrochemical impedance spectroscopy, potentiometry, and other electrochemical techniques [2-8]. Kim et al. selected DNA aptamers that specifically bind to estradiol through the SELEX (Systematic Evolution of Ligands by Exponential enrichment) process from a random ssDNA [8]. In this study, the DNA aptamer was immobilized on the gold electrode and estradiol was detected using a redox mediater where the sensitivity and selectivity of the detection was drastically increased. In an another study, Zhu et al. [9] successfully developed a new highly sensitive, selective, and label free sensor for the detection of anticancer drug “daunomycin” using a cancer cell surface lipid molecule “phosphatidylserine” and aptamer co-immobilized onto the nanocomposite conducting polymer film [7]. This sensor was 190 times more sensitive than the then reported any other daunomycin detection system. The strategy described in this work has many attractive features, such as: simplicity, rapidity, no requirement for a specific label (i.e., a fluorescent or reactive moiety), low-cost and hence could be a useful method in medical diagnosis.

Aptamers have not only been utilized for the detection of biochemical molecules rather it has been very well explored for the detection of cancer cells. Zhu et al reported a novel method to detect human epidermal growth factor receptor 2 (HER2) and HER2- overexpressing breast cancer cells using an electrochemical immunosensor combined with hydrazine and aptamer-conjugated gold nanoparticles (AuNPs) conjugate [10]. The conjugate selectively deposited the silver ion only on the immunocomplex and hence helped in the highly selective bioimaging of cancer cells by bare eye and under optical microscope in a very short time. This method exhibited an excellent diagnosis method for the ultrasensitive detection of SKBR- 3 breast cancer cells in human serum samples. In another study, a novel electrochemical aptamer sensor for the detection of acute blood cancer, Burkitt’s lymphoma has been reported (Figure 1). In this case, cancer cell is firstly captured by an aptamer probe, and the cell-aptamer complex was analyzed by an electrochemical detection process through the alkaline phosphatase-catalyzed silver deposition reaction [11]. It is expected that this aptamer based detection is simple and cost-effective, and exhibits excellent compatibility with miniaturization technologies in future.


Figure 1: Schematic diagramofan aptamer sensor, various kinds of clinically important target molecules, and detection using electrochemical techniques.

A part of electrochemical detection methods, aptamer has also been coupled with microfluidic device for the extraction and detection of various biomolecules and cancer cells. This integrated technology is extremely advance in clinical analyses and it could be a next generation point-of-care assay system. In a study, a microfluidic device for specific extraction and thermally activated release of analytes using nucleic acid aptamers has been reported [12]. The device primarily consists of a microchamber packed with aptamer-functionalized microbeads as a stationary phase, and integrated with a micro heater and temperature sensor. The device functioning has been verified by performing the extraction of a metabolic analyte, adenosine monophosphate coupled with thiazole orange, with high selectivity. In another case, circulating cancer cells were isolated from whole blood using an aptamer mediated micropillar-microfluidic device [13]. The special geometry of the micropillar array in the device resulted in the high-performance circulating cancer cell isolation. This microfluidic device enabled the isolation of as few as 10 tumor cells/1 mL of whole blood within half an hours. The advantages of such aptamer mediated device over the other methods include rapid analysis, no pre-treatment of blood samples, and low detection limit. Consequently, this aptamer-coupled microfluidic device has a potential to be used for clinical applications such as cancer diagnosis, prognosis, and monitoring the progress of therapeutic treatment.

In conclusion, electrochemical aptamer based methods have been very well developed in clinical diagnostics and therapeutics. Various kind of novel sensor fabrication approaches and new aptamer design will be extremely helpful for highly sensitive and selective analysis of clinical analyte in future.


Dr. Pranjal Chandra thanks to Amity Institute of Biotechnology, Amity University Uttar Pradesh for the university research grant.


Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Article Usage

  • Total views: 11625
  • [From(publication date):
    August-2013 - Sep 26, 2017]
  • Breakdown by view type
  • HTML page views : 7837
  • PDF downloads :3788

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version