Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Science, Jilin University, China
Received date: November 22, 2016; Accepted date: November 30, 2016; Published date: December 07, 2016
Citation: Huang Y (2016) Anticancer Activity and Mechanism of Alpha-Helical Antimicrobial Peptide. Drug Des 5:e133. doi:10.4172/2169-0138.1000e133
Copyright: © 2016 Huang Y. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Visit for more related articles at Drug Designing: Open Access
Anticancer peptides (ACPs) have become promising molecules as new anticancer agents due to the unique mechanism and several extraordinary properties, such as smaller size, high activity, low immunogenicity, good biocompatibility, etc. .
HPRP-A1 is an 15 amino acid residues antimicrobial peptide (AMP), derived from the N-terminus of ribosomal protein L1 (RpL1) of Helicobacter pylori . It can be induced to form an amphipathic α- helical structure in hydrophobic medium and exhibited good antimicrobial and antifungal activity [2,3]. Based on the mechanism of antimicrobial peptides, they can descript the integrit of cell membrane by the electrostatic interactions and hydrophobic interaction. HPRPA1 has been also found that can induce HeLa cell apoptosis by both the extrinsic and intrinsic pathways of the caspase cascade . In order to improve the penetrability and specificity against cancer cell lines, a new hybrid peptide of HPRP-A1-TAT has been designed and synthesized by linked a cell-permeating peptide TAT to the C-terminus of HPRP-A1 . Compared to HPRP-A1, the hybrid peptide of HPRPA1- TAT exhibited higher anticancer activity against HeLa cells with lower toxicity against human RBC by increasing early apoptosis of HeLa cells and inducing caspase activity. Furthermore, we explored combinational anticancer therapy using HPRP-A1 with the chemical drugs doxorubicin (DOX). The data showed that the anticancer activity of these drugs against HeLa cell lines was synergistically increased both in vitro and in vivo . We think this strategy of combination therapy appears to have great clinical potential.
Recently, we are working on the targeting modification of HPRP-A1 and the polymer modification to improve the selectivity and stability. We believe that ACPs as novel anticancer drugs will be play an important role for the clinical practices.