alexa Antimicrobial Activity and Fatty Acids from Ipomea ochraceae

ISSN: 2161-0444

Medicinal Chemistry

  • Research Article   
  • Med chem (Los Angeles) 2017, Vol 7(10): 299
  • DOI: 10.4172/2161-0444.1000471

Antimicrobial Activity and Fatty Acids from Ipomea ochraceae

Idowu OT* and Ojo OA
Department of Industrial Chemistry, Afe Babalola University, Afe Babalola Way, Ado Ekiti, Nigeria
*Corresponding Author: Idowu OT, Department of Industrial Chemistry, Afe Babalola University, Afe Babalola Way, Ado Ekiti, Nigeria, Tel: +2348037400826, Email: [email protected]

Received Date: Oct 03, 2017 / Accepted Date: Oct 16, 2017 / Published Date: Oct 21, 2017

Abstract

Ipomea ochraceae is from the family Convolvuliacea. It is mainly referred to as weed. The whole plant was air-dried, crushed into powdery form and then extracted using ethanol. The purpose of this study is to determine its antimicrobial activity and fatty acids from the constituents derived from GC-MS analysis.

Keywords: Ipomea ochraceae; Fatty acids; Antimicrobial activity

Introduction

The family Convolvulaceae includes about 57 genera and 1625 species [1]. It is also called morning glories have many special meanings to different people. They decorate our world with beautiful flowering vines and provide us with some nutritious and delicious vegetables. Morning glories are splendid for enhancing fences or for covering up eyesores. They rapidly cover fences, arches, pergolas, and trellises or can be made into their own garden feature with stakes and twine [2].

Fatty acids, both saturated and unsaturated play a key role in metabolism, as a metabolic fuel, as a necessary component of all membranes and as a gene regulator. In addition, fatty acids have a number of industrial uses [3]. Obtaining fatty acids from plant sources is more beneficial for one’s health. A well-planned plant based diet fatty acids rich food promotes heart health, ensuring the proper growth and development of your child and relieving pain. In this present study, focus shall be mainly on the antimicrobial activity and fatty acids from Ipomea ochracea [3].

Materials and Methods

Plant material and general experimental methods

Fresh leaves of Ipomea ochracea were collected in the month of November, 2016 from Afe Babalola University, Ado-Ekiti (ABUAD) Ekiti state, Nigeria. The plant was authenticated by the herbarium staff of the Botanical department at the Ekiti State University.

Sample preparation

950 g of the leaves were air dried at room temperature for about seven days. Thereafter, the dried leaves were pulverized and extracted using 95% ethanol at room temperature. The extracts were concentrated to dryness and the residue were obtained as a greenish black solid, after which the residues were transferred into a pre-weighed sample containers and were stored and later used for phytochemical screening and antioxidants activity.

Antimicrobial screening

The agar diffusion (cup plate) method was used for this examination. Molten and cooled agar 60 ml (45°C) were separately inoculated with the nutrient broth culture of the test organisms (0.6 ml) and mixed thoroughly. The inoculated medium was then carefully poured into sterile Petri dishes (24 cm Petri dish) and allowed to set. Thereafter, cups (9 mm diameter) were aseptically bored into the solid nutrient agar using a sterile cork borer. The test solutions 100 μL each were then introduced into each of the cups ensuring that no spillage occurred.

Also, the same volume of the standard antimicrobial agent and the solvent were introduced into some of the cups to act as positive and negative controls respectively. The plates were left at room temperature for 2 hours to allow for diffusion into the medium and thereafter incubated face upwards at 37°C for 24 hours. Sample was tested in duplicate and the diameters of zone of inhibition were measured to the nearest millimeter using a transparent ruler.

GC-MS

The GC was carried out using Hewlett- Packard 6890 GC series equipped with FID and HP-5 capillary column (cross linked 5% diPh, 95% dimethylpolysiloxane, 30 m × 0.32 mm i.d.; 0.25 μm film thickness). The column temperature was programmed at 50-210 at a rate of 3°C/ min. The injector and detector temperatures were 220°C and 270°C, respectively. Samples (1 μL of the oil solutions in chloroform, 2 mg/ mL) were injected by the split less technique into nitrogen carrier gas (0.8 mL/min).

Results

The results have been explained with the aid of Plates 1 and 2 (Tables 1 and 2).

medicinal-chemistry-Antimicrobial-activity

Plate 1: Antimicrobial activity of Ipomea ochracea (Ethanol extract).

medicinal-chemistry-Hexane-extract

Plate 2: Antimicrobial activity of Ipomea ochracea (Hexane extract).

Samples E1 E2 CnE H1 H2 CnH
B. cereus
NCIB 950
16.0(S) 11.0(S) 0.0(R) 10.0(S) 10.0(SR) 0.0(R)
E. coli
NCIB 86
0.0(R) 0.0(R) 0.0(SR) 0.0(R) 0.0(R) 0.0(R)
S. aureus
NCIB 8588
18.0(S) 20.0(S) 0.0(R) 13.0(S) 16.0(SR) 0.0(R)
Serratia mercescens
NCIB 1377
0.0(R) 0.0(R) 0.0(R) 18.0(SR) 24.0(SR) 0.0(R)
Aspergillus flavus 0.0(R) 0.0(R) 0.0(R) 0.0(R) 0.0(R) 0.0(R)
Fusarium species 0.0(R) 0.0(R) 0.0(R) 0.0(R) 0.0(R) 0.0(R)
Penicillium halicum 0.0(R) 0.0(R) 0.0(R) 0.0(R) 0.0(R) 0.0(R)
Trinchodern vinde 0.0(R) 0.0(R) 0.0(R) 0.0(R) 0.0(R) 0.0(R)
Trichrophytn rubrum 0.0(R) 0.0(R) 0.0(R) 0.0(R) 0.0(R) 0.0(R)
Candida alblicans 22.5(R) 25.0(R) 0.0(R) 11.0(R) 14.0(R) 0.0(R)

Table 1: Zones of inhibition (diameter mm; less cup size) of Ipomea ochraceae crude ethanol and hexane extracts at 100 mg/ml and 25 mg/ml.

Peak Retention time Area Name
1 9.017 0.24 2,4-Pentadienoic acid, 1-cyclopenten-3-on-1
2 9.789 0.55 n-Decanoic acid
3 10.11 0.31 Decanoic acid, ethyl ester
4 10.22 0.15 1-Pentanol, 4-methyl-2-propyl
5 10.66 0.39 Bicyclo[7.2.0]undec-4-ene,4,11,trimeth
6 11.42 0.15 1,6-cyclodecadiene, 1-methyl
7 11.75 0.28 Tridecanoic acid, methyl ester
8 12.36 8.92 Dodecanoic acid
9 12.64 5.17 Dodecanoic acid, ethyl ester
10 15.32 6.06 Tetradecanoic acid
11 15.69 3.31 Tetradecanoic acid, ethyl ester
12 15.81 0.23 Sulfurous acid, decyl 2-propyl ester
13 17.55 1.26 Cis-9-Hexadecenoic Tetradecanoicacid, ethylester acid
14 17.82 24.07 n-Hexadecanoic acid
15 18.70 0.17 9,9 Dimethoxybicyclo[3,3,1] nona-2,4-dione
16 19.04 0.59 9,12-Octadecadienoic acid
17 19.25 0.45 Phytol
18 19.51 27.04 9-Octadecenoic acid,(E)
19 19.71 9.72 Octadecanoic acid
20 20.70 0.84 Erythro-9,10-Dibromopentacosane
21 21.286 1.91 Eicosanoic acid
22 21.70 0.12 Erythro-9,10-dibromopentacosane
23 22.317 1.65 Hexadecanoic acid, 1(hydroxymethyl)-1,2-ethanediyl
24 22.591 4.20 Diisooctylphthalate
25 22.765 2.25 Eicosanoic acid, 2-[(1-oxohexadecyl)oxy]-1

Table 2: Fatty acids from Ipomea ochracea.

Discussion

Ipomea ochracea ethanol extract was moderately active against B. cereus at the zone of inhibition 16 mm but its hexane extract was low at 10 mm. Its ethanol extract was strongly active against S. aureus at the zone of inhibition 18 mm but its hexane extract was low at 13 mm. Ipomea ochracea was not active against all other fungi mentioned from Table 1 except Candida alblicans. Ipomea ochracea ethanol extract was strongly active against Candida alblicans at the zone of inhibition 22.5 mm but showing low activity at 11 mm. This plant can be used to treat various infections caused by B. cereus and S. aureus. The potency of the extract was strong in the polar portion than the non-polar portion.

Twenty-five compounds are present in the Ipomea ochracea which are majorly the fatty acids. Application of phytol can be used as one of the drugs that can be used to treat fluke’s infestation.

Conclusion

The crude extract of Ipomea ochracea showed strong antimicrobial activity for the first time. The constituents of the plants revealed some fatty acids like phytol, eicosanoic acids and so on for the first time. Further investigation could be carried out to isolate the active fatty acids that could be used in the pharmaceutical industry.

References

Citation: Idowu OT, Ojo OA (2017) Antimicrobial Activity and Fatty Acids from Ipomea ochraceae. Med Chem 7: 299-301. Doi: 10.4172/2161-0444.1000471

Copyright: © 2017 Idowu OT, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Select your language of interest to view the total content in your interested language

Post Your Comment Citation
Share This Article
Recommended Conferences
Viewmore
Article Usage
  • Total views: 331
  • [From(publication date): 0-2017 - Feb 25, 2018]
  • Breakdown by view type
  • HTML page views: 284
  • PDF downloads: 47

Post your comment

captcha   Reload  Can't read the image? click here to refresh