alexa Biomarker Detection for Neurodegenerative Diseases Using Streptomycin | Open Access Journals
ISSN: 2157-7064
Journal of Chromatography & Separation Techniques
Like us on:
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Biomarker Detection for Neurodegenerative Diseases Using Streptomycin

Aly Moussa*

Anses Lyon laboratory, 31 Avenue Tony Garnier, 69007 Lyon, France

*Corresponding Author:
Aly Moussa
Anses Lyon Laboratory
31 Avenue Tony Garnier, 69007 Lyon, France
Tel: 33 617460952
E-mail: [email protected]

Received date: December 16, 2013; Accepted date: January 27, 2014; Published date: January 30, 2014

Citation: Moussa A (2014) Biomarker Detection for Neurodegenerative Diseases Using Streptomycin. J Chromatograph Separat Techniq 5:210. doi:10.4172/2157-7064.1000210

Copyright: © 2014 Moussa A. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Journal of Chromatography & Separation Techniques

The prion protein structure contains a spherical core sequence with a generally highly negatively charged surface sequences and at least two positively charged pockets rich in lysine or arginine residues. Conformational changes in the cellular prion glycoprotein PrPc leads to propagation of a beta-sheet conformed, detergent insoluble and Proteinase K (PK) resistant pathogenic protein, PrPsc.

The accumulation of this PrPsc in animals and man is associated with a wide range of Transmissible neurodegenerative Spongiform Encephalopathies (TSES), including scrapie in sheep, spongiform encephalopathy in cattle, chronic wasting disease in deer and Creuztfeldt-Jakob disease in humans (CJD). The recent discovery of a new variant of Creuztfeldt-Jakob disease transmissible by blood transfusion requires tests that allow detection of PrPsc at very low concentrations in biological fluids.

Early laboratory diagnosis of diseases relies on the employed technique sensitivity for the detection of the corresponding disease biomarker. The use of streptomycin in the detection procedures of the pathogenic prion protein represents a new and attractive way to detect more PrPsc, the best biomarker for the TSEs.

The addition of increasing quantities of streptomycin to constant amount of brain extracted and PK treated PrPsc, followed by electrophoresis on 15% polyacrylamide gel, transfer and immunodetection revealed that the molecular mass increase for each of three PrPsc bands was proportional to the quantity of the added streptomycin. When higher concentrations of streptomycin were used, it causes flocculation and aggregation of the PrPsc which can then be separated by a low centrifugation step. Also the addition of increasing quantities of streptomycin to the Alzheimer peptide P53 revealed an increase of its molecular mass in the same manner as with PrPsc [1,2].

The supposed mechanism of interactions between streptomycin and the prion proteins in vitro is suggesting occurring through hydrogen bound transfer between the 2 guanidine groups present on the streptomycin molecules and the amino acids of one or several prion proteins thus forming multimolecular aggregates. Analysis of molecules sharing common chemical functions with streptomycin and reproduced aggregation and precipitation of the PrPsc ruled out the possibility of a shift-base reaction. Examples of such functional equivalence was shown also for Dihydro streptomycin, bis-3-aminopropylamine, guanidine hydrochloride and spermine tetrahydrochloride.


Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Recommended Conferences

Article Usage

  • Total views: 11601
  • [From(publication date):
    February-2014 - Sep 27, 2017]
  • Breakdown by view type
  • HTML page views : 7820
  • PDF downloads :3781

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version