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ISSN: 2155-9600
Journal of Nutrition & Food Sciences

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β-carotene Content of Some Commonly Consumed Vegetables and Fruits Available in Delhi, India.

Richa Pritwani* and Pulkit Mathur

Department of Food and Nutrition, Lady Irwin College, University of Delhi, New Delhi, 110001, India

*Corresponding Author:
Richa Pritwani
Department of Food and Nutrition
Lady Irwin College
University of Delhi
Sikandra Road
New Delhi, 110001, India
Tel: +91-9540301188
E-mail: [email protected]

Received Date: July 22, 2017; Accepted Date: August 09, 2017; Published Date: August 16, 2017

Citation: Pritwani R, Mathur P (2017) β-carotene Content of Some Commonly Consumed Vegetables and Fruits Available in Delhi, India. J Nutr Food Sci 7:625. doi: 10.4172/2155-9600.1000625

Copyright: © 2017 Pritwani R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Most of the vitamin A in the diet comes from plant food sources in developing countries. This study was designed with an objective of determining β-carotene content of a total of 26 types of green leafy vegetables, tubers, other vegetables and fruits obtained from four wholesale markets in Delhi, India using HPLC. There was a wide variation in β-carotene content of green leafy vegetables, with means ranging from 2199 μg/100 g in Basella rubra to 7753 μg/100 g in Amaranthus gangeticus. A large variation was observed in β-carotene content of fruits and the mango varieties tested, ranging from undetectable levels in strawberry and 808.60 μg/100 g in totapuri mango up to 11789 μg/100 g in alphonso mango. Approximately 65 g and 100 g of a green leafy vegetable would meet daily requirement of a preschooler and older child/adult respectively. Mango has considerable amount of β-carotene, and consuming a medium-sized bowl by preschool children would meet 99% of Recommended Dietary Allowances (RDA). The information generated is useful in identifying types of fruits and vegetables with higher concentration of the provitamin A in low income economies where fruits and vegetables are expensive. Individuals need to consume only small quantities of these vitamin A rich foods to meet daily requirement.


β-carotene; Green leafy vegetables; Fruits; Mango varieties; HPLC; Portion sizes


Vitamins A are essential for normal vision, maintaining the integrity of epithelial tissues and for a wide variety of other metabolic functions. Micronutrient malnutrition especially deficiency of vitamin A is globally affecting over 3 billion people. According to World Health Organization (WHO), Vitamin A Deficiency (VAD) has affected about 190 million preschool-aged children and 19 million pregnant women, mostly in Africa and South-East Asia [1]. Prevalence of subclinical vitamin A deficiency is around 62% in preschool children in India [2,3].

Studies from developing regions suggest that up to 80% of the dietary intake of vitamin A comes from provitamin A rich food sources. Vitamin A occurs as provitamin A carotenoids, which are synthesized as pigments by many plants and are found in green, orange, and yellow plant tissues. These provitamins or precursors-alpha- carotene, beta-carotene, gamma-carotene, and cryptoxanthine (all of which contain β-ionone rings) are found in plant foods. In animal food products their presence can be explained by the fact that animals consume plants rich in these provitamins. There are more than 500 different carotenoids out of which about 60 have provitamin A activity. These can be cleaved by animals to yield at least one molecule of retinol. In practice, however, only five or six of these provitamins are detected in commonly consumed foods of which β-carotene is the most predominant and active [4].

A food based approach is best to combat vitamin A deficiency among groups at risk of deficiency. The poorer segments of the population in India are dependent on plant foods, which provide β-carotene to meet their requirements of vitamin A. Green Leafy Vegetables (GLVs) are grown abundantly in India and are relatively inexpensive. However, these are not liked by most, especially children. Other vitamin A rich fruits and vegetables are relatively more expensive. β-carotene content of some commonly consumed vegetables and a fruit was estimated to identify types which had the highest amount of the pro-vitamin A. The quantity of these food items which would contribute to meeting the requirement of children and adults was also assessed.

Materials and Methods

Method of sampling of fruits and vegetables

About 161 samples of commonly consumed sources of β-carotene like 13 types of green leafy vegetables (n=84 samples), 3 types of roots and tubers (n=12 samples), 3 types of other vegetables (n=10 samples), 12 varieties of mango (n=34 samples), and 6 types of other fruits (n=21 samples) were purchased from four different whole sale markets (mandis) of Delhi. These included Azadpur (north Delhi) supplying fruits and vegetables to a significant part of Delhi, Okhla mandi (south Delhi), Shahadra mandi (east Delhi) and Keshopur mandi (west Delhi). Each type and variety of fruit and vegetable was picked up from three different vendors and locations in each market. These samples were pooled and homogenised to give a uniform single composite sample representing each market. Then these samples were analysed for β-carotene content in duplicate.

Laboratory estimation of β-carotene

Extraction: For the extraction of β-carotene, the procedure outlined in AOAC Official Method 941.15- ‘Carotene in Fresh Plant Materials and Silages’ [5] was followed. Samples were finely cut with scissors or knife, ground in mortar pestle and 2–5 g weighed test portion was extracted with 40 ml acetone, 60 ml petroleum ether, and 0.1 g magnesium carbonate, and then blended for 5 min. Filtration was done with the aid of a suction pump and sample was decanted into separator. Residue was washed with two 25 ml portions acetone, then with 25 ml petroleum ether, and then extracts were combined. The combined extract was evaporated to dryness and residue was re-dissolved in acetone. Volume was made up to 1 to 5 ml using acetone depending upon the matrix. The sample was then analyzed by high performance liquid chromatography (HPLC).

HPLC analysis: The HPLC instrumentation included a 5 μm C18, 4.6 × 250 mm (Varian Associates, Sunnyvale, USA) for β-carotene analyses. A mixture of acetonitrile (CH3CN), methanol (CH3OH) and acetone in the ratio 60:30:10 served as the mobile phase for β-carotene analysis at a flow rate of 1.2 ml per minute. Peak responses of β-carotene were measured at wavelengths of 450 nm [6]. Pure standards of all-trans-β-carotene were obtained from Sigma Aldrich. Purity of the standards was checked by HPLC analysis. Working solutions of β-carotene were analyzed with each batch of samples on the day of analysis.

Quality control of the analytical methods: The method was validated with regard to accuracy, linearity and precision. Analytical testing was done in NABL accredited laboratory. Reproducibility was checked by extracting the sample three times and calculating recovery and Coefficient of Variance (CV). The recovery was checked by spiking the plant matrix with known amounts of pure standard of β-carotene. Good laboratory practices were followed to ensure appropriate sample preparation and analysis. All chemicals used were of HPLC grade.

Statistical analysis

The mean, standard deviation and range of β-carotene content for each fruit and vegetable was reported. All data were analyzed using the SPSS version 22 software. The differences in mean values were tested using one-way Analysis of Variance (ANOVA) and post hoc analysis using Tukey HSD test. The value of p<0.05 was considered to be statistically significant.

Results and Discussion

Results for recovery assays

Samples of plant matrices were spiked at the level of 20 mg/kg of pure β-carotene standard. Intra-laboratory reproducibility of the method was estimated by reporting mean recovery, standard deviation and CV. The percentage recovery of β-carotene was found to be 90.08 ± 2.2% with a CV or percent relative standard deviation (%RSD) of 2.52%.

Green leafy vegetables

There was a significant variation in β-carotene content of green leafy vegetables with the values ranging from 2199 μg/100 g in Poi leaves (Basella rubra) to 7753 μg/100 g in chaulai leaves (Amaranthus gangeticus) (p<0.05) (Table 1). Spinach leaves (Spinacia oleracea) had β-carotene in the range of 2966–3967 μg/100 g with the average value 3468 ± 296 μg/100 g. In Indian Food Composition Tables (IFCT) [7], the β-carotene content of spinach has been reported as 2605 ± 521 μg/100 g. The results of the present study were similar to the previously reported results ranging from 3000 to 5000 μg/100 g [8-13]. However, higher amounts of β-carotene in spinach leaves have also been reported ranging from 5000 μg/100 g to 152100 μg/100 g [14-19].

S. No. Food sample-Local/Botanical name Number of samples analyzed (n=84) β-carotene (µg/100g) range β-carotene* (µg/100g) Mean ± SD
1. Spinach leaves (Spinaciaoleracea) 20 2966-3967 3468 ± 297e
2. Fenugreekleaves (Trigonellafoenumgraecum) 10 4826-5838 5438 ± 373c
3. Mustard leaves(Brassica campestris var. sarason) 6 4224-4355 4327 ± 70d
4. Raddish leaves (Ruphanussativus) 6 3889-4504 4203 ± 251de
5. Mint leaves (Menthaspicata) 4 3872-4375 4206 ± 289de
6. Bathua leaves (Chenopodium album) 6 4233-4341 4300 ± 58de
7. Coriander (Coriandrumsativum) 4 5404-5728 5566 ± 229bcd
8. Curry leaves (Murrayakoenigii) 4 5403-5822 5612 ± 296bcd
9. Poi leaves or Mayalu (Basellaalba) 4 1926-2655 2199 ± 397f
10. Nadi/Kalmi leaves (Ipomeaaquatica) 4 6340-8083 7212 ± 1232ab
11. Chaulaileaves (Amaranthus gangeticus) 6 6418-8865 7753 ± 1104a
12. Colocasia Leaves (Colocasia anti-quorum) 6 6255-6729 6494 ± 199b
13. Kulfa leaves or paruppukeerai (Portulacaoleracea) 4 3588-3991 3789 ± 286de

Table 1: β-carotene content of some common GLV.

Coriander (Coriandrum sativum) had β-carotene in the range of 5404-5728 μg/100 g and results are in agreement with earlier published data [8,10,20]. Higher values of 67500 μg of β-carotene/100 g have been reported in coriander leaves in a study conducted in south India [21]. However, lower values of 3167-3808 μg of β-carotene/100 g have been reported in IFCT, US data base and a study of Malaysia [7,17,19]. Fenugreek leaves (Trigonella foenum graecum) had β-carotene in the range of 4826-5838 μg/100 g which is nearly similar to the amount of 4230-4350 μg/100 g reported by Indian authors earlier [22,23]. Although higher values of β-carotene in fenugreek leaves (9245 ± 974 μg/100 g) were reported in IFCT [7]. Other studies conducted in south India also had reported higher values of 9200 and 12130 μg of β-carotene/100 g respectively in fenugreek leaves [11,16].

In the present study β-carotene found in chaulai leaves (Amaranthus gangeticus) was in the range of 6418-8865 μg/100 g which was nearly similar to the values reported by authors of IFCT as 8553 μg/100 g [7]. Studies conducted in south India have also reported values (5760-8600 μg/100 g) of β-carotene in amaranth leaves nearly similar to values of present study [11,24,25]. While, one of the study conducted in Mysore, India have even reported higher values as 18670 μg of β-carotene/100 g of amaranth leaves than present study [21]. Lower values (1526-1709 μg/100 g) than present study have been reported in a study conducted in Bangladesh [26]. There are many edible varieties of amaranth in different parts of the world. It has been reported that Amaranthus viridis had β-carotene content in the range of 3200 μg/100 g to 58950 μg/100 g [11,19-21,24,27]. It has been reported that other species like Amaranthus cruentus from south India had 7600 μg of β-carotene content per 100 g [27] while Amaranthus tricolor had 1601 μg to 9600 μg of β-carotene/100 g reported in other studies [10-11,19,27-30].

The availability of greens improves during winters in Delhi. Mustard leaves (Brassica campestris var. sarason) are one of the most commonly consumed green leafy vegetables in North India during winters. The β-carotene content was found to be nearly similar in mustard leaves (4224-4355 μg/100 g), radish leaves (Ruphanus sativus)-3889-4504 μg/100 g, bathua leaves (Chenopodium album)–4233-4341 μg/100 g and mint leaves (Mentha spicata)-3872-4375 μg/100 g in the present study. In IFCT nearly similar amounts of β-carotene (3808 μg/100 g) have been reported in mint leaves [7]. The amounts of β-carotene in mustard leaves are comparable with those in green leafy Brassica species tested in Switzerland 2100–6800 μg/100 g [31]. Values lower than the present studies have also been reported for Indian mustard leaves-1680 μg/100 g [32] and 2619 ± 372 μg/100 g [7]. Authors from Bangladesh reported the β-carotene content of commonly consumed Bangladeshi vegetables. They reported 1404 ± 36.1 μg/100 g in mustard leaves and 1871 ± 875 μg/100 g in radish leaves which are lower than the results obtained in the present study [26]. Lower values (2300 and 2591 μg/100 g respectively) as compared to present study have been reported in radish leaves in IFCT and a study from south India [7,11] while very high content of β-carotene (11200 μg/100 g) was also reported in radish leaves by some Indian authors [16]. About 5200– 6300 μg of β-carotene/100 g were reported in 23 samples of Brassica oleracea cultigens of Durham, New Hampshire, USA [33].

Lower value of 1075 μg of β-carotene/100 g in bathua leaves has been reported in IFCT [7] while some other authors [21,27,34] have reported higher β-carotene content (9300 μg/100 g; 26380 μg/100 g and 114610 μg/100 g respectively) in bathua leaves as compared to the present study. Results of β-carotene in mint leaves (Menthaspicata) are in agreement with other previously published data [10,11]. However, wide variations in the β-carotene values (2133 μg to 10600 μg/100 g) have been reported in some other studies [14,17,21].

Less commonly consumed green leafy vegetables like curry leaves (Murrayakoenigii) had β- carotene in the range of 5403-5822 μg/100 g. However higher concentrations in the range of 7100-9328 μg/100 g have been reported in IFCT [7] and by other authors [11-19, 21,22]. Kulfa leaves or paruppu keerai (Portulacaoleracea) were found to be a good source of β-carotene (3588-3991 μg/100 g), with nearly similar amounts (3200 μg of β-carotene per 100 g) being reported by authors from Brazil [20]. Higher amounts of 27050 μg of β-carotene per 100 g in paruppu keerai of Mysore, India [21] have been reported. Lower values as compared to present study as 586 μg and 3200 μg of β-carotene per 100 g in paruppu keerai have been reported in a study from south India and southern Thailand respectively [35,36]. Poi leaves (Basella alba rubra) had lower amounts of β-carotene in the range of 1926-2655 μg/100 g, nearly similar to values reported in IFCT (2473 μg/100 g). However, very high values in the range of 32420-43820 μg/100 g have also been reported in previously published data [16,21]. The reason for difference in the carotenoids content could be because of the different geographical locations, inherited biological variability in the varieties and species or cultivar, part of the plant, degree of maturity/ripeness at harvest, cultivation conditions, seasonal variation, the effect of climatic conditions, variation of fertilizer, different soil conditions, postharvest handling practices, shelf time before purchasing, method for sampling, preparation and estimation [37].

Some less commonly consumed vegetables like Ipomea aquatica leaves (7212 μg/100 g) and colocasia leaves (6493 μg/100 g) had good amounts of β-carotene. Some Indian authors have also reported β-carotene of colocasia leaves as 5500 μg/100 g [11] and 5758 μg/100 g in IFCT [7]. Small amounts of these incorporated into diets of children can effectively increase intake of the vitamin and help in combating vitamin A deficiency.

Tubers and other vegetables

Provitamin A sources are green, red, yellow and/or orange colored vegetables like sweet potato, pumpkin, carrot, capsicum, green chillies. β-carotene content of various tubers and vegetables differed significantly (p<0.05) and has been presented in Table 2.

S. No. Food sample-Local/Botanical name Number of samples analyzed (n=22) β-carotene (µg/100g) range β-carotene (µg/100g)* Mean ± SD
Tubers (n=12)
1. Red Carrot (Daucuscarota) 4 913-1331 1187 ± 188b
2. OrangeCarrot(Daucuscarota) 4 1845-1970 1906 ± 52a
3. Sweet potato (Ipomeabatatas) 4 605-1810 708 ± 145c
Other Vegetables (n=10)
4. Green chillies (Capsicum annum) 2 980-1017 998 ± 26bc
5. Capsicum (Capsicum annum) (bell pepper) 2 33-40 36 ± 5d
6. Pumpkin (Cucurbita maxima) 6 405-520 470 ± 52c

Table 2: β-carotene of some tubers and other vegetables.

Two types of commonly consumed carrots were analyzed in this study. The mean concentration of β-carotene in red carrot (Daucus carota) was 1187 ± 188 μg/100 g while orange carrot had 1906 ± 52 μg/100 g. IFCT reported higher values in red and orange carrot (2706 and 5423 μg/100 g respectively) as compared to present study [7]. Also, the results of the present study for carrot were far lower than the other studies. There was a wide variation reported in the β-carotene contents starting from 2200 μg/100 g up to 128400 μg/100 g in earlier published studies [10-13,15,17,25,38-43].

Published results have reported wide disparity in the contents of β-carotene due to difference in varieties of sweet potato (Ipomea batatas) tested. The results of present study (605.33-1810 μg/100 g) are far lower than the previously published reports [44-47]. Amount of β-carotene was also found varying amongst ten clones of sweet potato (5.85 to 13.63 mg/100 g of fresh weight) possessing different intensities of dark orange-flesh color studied in India [48]. It was found in an Indian study that the variety-‘kiran’ (yellow fleshed) of sweet potato had a significant amount of β-carotene (approximately 1870 μg/100 g) [11]. β-carotene content was reported as 5376 μg/100 g in IFCT [7] and in the range of 9180 to 9500 μg/100 g in USDA-NCC Carotenoid Database [17].

In the present study, the concentration of β-carotene was found to be very low (36.33 ± 5.19 μg/100 g) in green pepper (capsicum annuum) and this result was in agreement with the previously published results ranging from 38 μg/100 g to 270 μg/100 g [8,11-13,17,36,40-49]. Authors have observed wide range of concentrations of β-carotene content-10.4 μg/100 g to 1524 μg/100 g in fresh red, yellow and orange peppers of Italy. β-carotene was significantly higher in concentration in red peppers as compared to yellow and orange peppers [50]. Other authors have observed a range of 2379–12450 μg/100 g of β-carotene in Capsicum annuum (red pepper) cultivars of US and Turkey [17,51] while in the European database it was reported as low as 480 μg/100 g [40]. Another study reported 153 μg/100 g of trans β-carotene in red peppers of Boston, USA [8]. In IFCT also low values of β-carotene-328, 166 and 246 μg/100 g was reported in green, yellow and red varieties of capsicum respectively [7].

Amount of β-carotene in green chillies (Capsicum annum) was in the range of 980-1017 μg/100 g. These results were in agreement with values ranging from 1020 to 1130 μg/100 g reported in Indian studies [11,36]. Although lower values (468 μg/100 g) have also been reported Malaysian green chillies [19]. In IFCT, amount of β-carotene was reported in the range of 207 to 294 μg/100 g in 7 different varieties of green chillies [7]. In the present study β-carotene levels detected in pumpkin (Cucurbita maxima) were in the range of 405- 520 μg/100 g. In IFCT the amount of β-carotene was reported in two types of pumpkin. Pumpkin, green, cylindrical had 363 μg/100 g while pumpkin, orange, round (Cucurbita maxima) had 149 μg/100 g [7]. Wide range of concentrations were obtained (310–2900 μg/100 g) in C. maxima in earlier studies [25,26,52,53]. In a study from Brazil, the range of β-carotene was found varying from 14195 to 24422 μg/100 g in two samples of raw pumpkins (C. moschata Duchesne) [54]. Authors determined β-carotene in three species of pumpkin (Cucurbita pepo, C. maxima and C. moschata) from Austria and found these to range from 1400 to 7400 μg/100 g [55]. It has been reported in other studies from Brazil, that the C. maxima ‘Exposição’ and C. moschata ‘Menina Brasileira’ had all-trans-β-carotene as the major carotenoid among primary carotenoids (α-carotene, β-carotene and lutein) [56,57]. A few varieties have α- and β-carotene as major carotenoids, whereas ß-carotene dominates in other varieties [57,58]. In Indian studies levels of β-carotene in pumpkins were in the range of 1160 to 1180 μg/100 g [11,36]. The concentration of β-carotene found in present study is much more than the 57.8 μg/100 g reported from Malaysia in C. maxima [19].


The values of β-carotene in fruits were ranging from undetectable levels in strawberry to 11789 μg/100 g in alphonso variety of mango (Table 3). β-carotene content in ripe tomato (Lycopersicon esculentum) was found to be 316.6-341.2 μg/100 g which were nearly similar to the results published in earlier studies [17,41]. Other authors have reported values in the range of 415 μg/100 g up to 740 μg/100 g [11-13,40,42]. In IFCT values of β-carotene were reported as 1513 μg/100 g in tomato, ripe, hybrid and 905 μg/100 g in tomato, ripe, local varieties [7]. β-carotene ranged from 280 to 620 μg/100 g in 12 varieties of Hungarian salad tomato [58]. Low concentration of 59.7 μg/100 g has been reported in tomato from Hyderabad, India [36]. In a study from Boston USA, negligible amounts of β-carotene were reported in tomato [8].

S. No. Food sample-Local/botanical Name Number of samples analysed (n=21) Β-Carotene (µg/100 g) range β-carotene (µg/100g)* Mean ± SD
1. Papaya (Carica papaya) 6 153-219 185 ± 34c
2. Orange(Citrus aurantium) 4 13-49 32 ± 19d
3. Guava(Psidiumguajava) 2 27-45 36 ± 13d
4. Tomato (Lycopersiconesculentum) 5 317-341 332 ± 14b
5. Cape gooseberry (Physalisperuviana) 2 775-858 816 ± 58a
6. Strawberry(Fragariavesca) 2 #ND (DL-4) -

Table 3: β-carotene of some common fruits.

The β-carotene content of papaya (Carica papaya) averaged 185.02 ± 34.28 μg/100 g and ranged from 153.2-219 μg/100 g in the present study, which were similar to the previously published values [17,19,42]. β-carotene was estimated as 190 μg/100 g to 560 μg/100 g in fresh papaya pulp in a study in Brazil [59]. However, the average values reported were higher in IFCT (694 μg/100 g) and also in other studies which reported mean values as 471 μg/100 g [60] and 440 μg/100 g [61].

β-carotene levels ranging from 80.5 to 410.3 μg/100 g were reported among 5 cultivars of papaya belonging to different locations grown in Hawaii [62]. It was observed that the β-carotene was significantly high in red fleshed papaya (700 μg/100 g DW) as compared to yellow-fleshed papaya (140 μg/100 g DW) [63]. The reasons suggested for the wide range in the β-carotene values were varietal differences, differences in maturity and ripening.

The amount of β-carotene in Orange (Citrus aurantium) was in the range of 12.62-49.63 μg/100 g. The results were in agreement with the earlier reported studies [7,11,12,17,40,61]. A study from Thailand found β-carotene content as 211 μg/100 g in sainahmphung variety of orange [60]. Guava (Psidium guajava) had 26.9-44.8 μg/100 g which was higher than 1 μg/100 g reported in guavas from south India [11]. The value was however lower as compared to 984 μg/100 g reported in guavas from Indonesia [61] and 298 μg/100 g in white flesh guavas and 267 μg/100 g in pink flesh guavas reported in IFCT [7]. The amounts of β-carotene in fruits determined in this study were nearly similar to those previously reported [52,61,17,19].

In our study β-carotene content was not quantifiable in strawberry (Fragaria vesca), being present at levels below detection limit (DL-4 mcg/100 g). In Cape gooseberry (Physalis peruviana) the β-carotene content was in the range of 775.21-857.71 μg/100 g. A study from Bulgaria has reported 4.9 μg of β-carotene/100 g of strawberries [64]. Strawberries from Norfolk, UK had 11 μg of β-carotene/100 g [12], and 8 μg/100 g was reported in the European database [40]. Authors of IFCT also reported very low values of 2.19 μg/100 g [7].

A large variation in amounts of β-carotene was observed in the mangoes (Mangifera indica) tested (Table 4). The values were ranging from 808.60 μg/100 g in totapuri mango up to 11789 μg/100 g in alphonso mango. Other authors have observed as much as 13000 μg/100 g in alphonso varieties from India [65]. Highest values were obtained in alphonso variety. The lowest values were obtained in the present study in totapuri, chausa, gola, kesar and pairy variety (808.60 to 1284.5 μg/100 g). The values are nearly similar to the results reported in the European carotenoid database (1300 μg/100 g) [40]. In IFCT amount of β-carotene was reported in 7 varieties (banganapalli, gulabkhas, himsagar, kesar, neelam, paheri and totapari) of mango in the range of 602 to 1291 μg/100 g [7]. Kesar and totapari had 1264 and 602 μg/100 g respectively which is similar to the results obtained in present study. For the varieties dinga, langda, safeda, sinduri, dusheria range of values (1632.91 -2456.66 μg/100 g) was obtained in the present study, which is similar to 1700 μg/100 g reported by other Indian authors [11]. Low values of 445 μg/100 g has been reported in mangoes in US database [17], 615 μg/100 g in mangoes from Malaysia [19], 553 μg/100 g in mangoes from Indonesia [61] and 142 μg/100 g in mangoes of USA were also reported [8]. A study from Thailand reported β- carotene in the range from 21.2 to 308 μg/100 g in different mango varieties [60]. Hence a wide variation can be observed in the amounts of β-carotene present in mango due to availability of so many varieties. Consuming mangoes with a low amount of vitamin A will not contribute majorly to the daily intake of this vitamin unless a sufficient quantity is consumed. However, mangoes with a higher β-content will definitely contribute to meeting daily requirements even if consumed in small quantities.

S. No. Name Number of β-carotene (µg/100g)  β-carotene (µg/100g)*
samples range Mean ± SD
analysed (n=34)
1. Alphonso 2 10775-11789 11282 ± 717a
2. Safeda 4 1875-2208 2031 ± 179bc
3. Sinduri 4 2099-2310 2204 ± 149bc
4. Langda 4 1720-1911 1787 ± 107bcd
5. Chausa 4 950-1282.19 1018 ± 237de
6. Totapuri 2 745.21-872 809 ± 90e
7. Gola 2 1200-1234 1217 ± 24cde
8. Dinga 2 1576-1689 1633 ± 79bcde
9. Dusheri 4 2000-2840 2457 ± 425b
10. Kesar 4 1108-1335 1222 ± 160cde
11. Pairy 2 1280-1290 1284 ± 8cde

Table 4: β-carotene of some common varieties of mango (Mangifera indica).

Typical portion sizes of fruits and vegetables

Quantities of raw fruits or cooked vegetables to be consumed to meet Recommended Dietary Allowances (RDA) for β-carotene were standardized using household measures and have been presented in Table 5. Percent RDA met from consuming one bowl of raw fruit or cooked vegetable was also calculated. The average β-carotene estimated in green leafy vegetables was 4966 μg/100 g. On an average daily consumption of 100 g of a green leafy vegetable would be enough to meet more than 100 % RDA among children and adults. When taking amaranth leaves, the GLV with highest concentration of β-carotene (that is 7753 μg/100 g), only 62 g needs to be consumed to meet daily requirement of an adult and young child aged between 7 to 9 years. For children less than 7 years, 42 g of raw amaranth leaves would be enough for meeting the RDA of β-carotene. Consuming 140 g of spinach by adults and children (7 to 9 years) and 93 g of spinach by preschool children (1 to 6 years) would be able to meet the RDA of β-carotene. 100 g of a GLV when cooked would fill half a medium sized bowl (200 ml) as a dry preparation.

S. No. Vegetable/Fruit Average β-carotene (µg/100g) Raw quantity to be consumed to meet RDA* (in grams)- Quantity of raw fruit or cooked vegetableto be consumed to meet RDA in household measures# Raw quantity of fruit or vegetable^fitting in a medium bowl (g) Percent RDA met from consuming one bowl of raw fruit or cooked vegetable
Preschool children (1-6 years) Other children and adults Preschool children (1-6 years) Other children and adults Preschool children (1-6 years) Other children and adults
1. All GLVs (average) 4967 65 97 Little more than 1/4th bowl Approximately half a bowl 205 318 212
2. Amaranth leaves (highest amount of β-carotene) 7753 42 62 Little less than 1/4th bowl Little more than 1/4th bowl 205 497 331
3. Spinach leaves (most commonly consumed) 3468 93 140 Half of a bowl Little less than 3/4th of bowl 205 222 148
4. Carrot 1187 275 400 Little more than 2 bowls 3 bowls 130 41 27
5. Sweet potato 708 452 680 3 bowls 5 bowls 140 31 21
6. Pumpkin 470 682 1022 Little more than 3.5 bowls Little less than 5.5bowls 190 28 19
7. Papaya 185 1760 2640 14 bowls 21 bowls 125 7 5
8. Tomato 332 965 1450 Little more than 5 bowls Little more than 7.5 bowls 185 19 13
9. Cape gooseberry 816 395 590 3 bowls Little less than 5bowls 125 32 21
10. Mango (average) 2450 155 200 Little more than a bowl One and a half bowl 130 99 66

Table 5: Standardization of quantities to be consumed to meet RDA and estimated vitamin A contribution of an average portion size of fruits and vegetables to the RDA for all age groups.

In case of other vegetables, the amount of β-carotene was less; hence more number of servings would be required to meet RDA. Percent RDA met from one medium bowl was less than 50% in case of carrot, pumpkin and sweet potato. In case of fruits, β-carotene amount in papaya was found to be very less and therefore higher number of servings would be required to meet RDA. However, since one vegetable or fruit need not contribute to 100% of the requirements, one serving contributing around 25-30% of the RDA, can be considered as a good source. Fruits and vegetables having low amounts would have to be eaten in unreasonably large amounts thus multiple sources of vitamin A in the diet will help to meet requirements. For instance, only 5- 7 percent of RDA would be met after consuming one medium bowl of papaya. Mango has considerable amount of β-carotene, and consuming a medium bowl would be sufficient to meet 99 % of RDA of preschool children.

Children are fussy about eating their vegetables. Vegetables can be incorporated in dishes in forms acceptable to children or by disguising their presence. For instance, GLVs can be kneaded into the dough of breads and other vegetables can be pureed/mashed and used as the base for gravies or as stuffing. Preparing meals for children with small quantities of vegetables yet higher concentration of β-carotene may be a sensible approach to reduce risk of deficiency of vitamin A among them (Tables 4 and 5).


Wide variation was observed in the values of selected fruits, green leafy and other vegetables consumed in Delhi. Among green leafy vegetables the highest amount of β-carotene was found in amaranth leaves followed by Ipomea aquatica leaves and colocasia leaves. Knowing that young children are prone to vitamin A deficiency, this information can be used by mothers to plan their meals by including the minimum amount of β-carotene rich fruits and vegetables in dishes. These can be incorporated in their meals in forms acceptable to the children.


The corresponding author is in receipt of a research fellowship from University Grants Commission, India; however it had no role in writing of this research paper. The authors thank University Grants Commission, India for the fellowship. The authors declare that they have no conflict of interest.


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