alexa Clinical Chemistry | Open Access Journals
ISSN: 2157-7064
Journal of Chromatography & Separation Techniques
Like us on:
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Clinical Chemistry

Aly Moussa*
National Agency for Sanitary Environmental Laboratoire de Lyon, France
Corresponding Author : Aly Moussa
National Agency for Sanitary Environmental Laboratoire de Lyon, France
Tel: 336-174-609-52;
E-mail: [email protected]
Received May 21, 2014; Accepted May 24, 2014; Published May 27, 2014
Citation: Moussa A (2014) Clinical Chemistry.J Chromat Separation Techniq S2:e001 doi:10.4172/2157-7064.S2-E001
Copyright: © 2014 Aly Moussa. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related article at
DownloadPubmed DownloadScholar Google

Visit for more related articles at Journal of Chromatography & Separation Techniques

Editorial
Clinical chemistry originated in the late 19th century for analyzing body fluids using simple chemical tests. A great progress was achieved later in the area of clinical pathology; with the development of another laboratory and biochemistry techniques such as chromatography, spectrophotometry, electrophoresis, immunoassay, molecular biology, proteomics and other separation techniques; and their use in fundamental and applied research. Today the obtained information with the application of these modern techniques progressively helped in resolving the recently developed medical life problems.
During this century, it was possible to explain the increasing number of diseases (Alzheimer and Parkinson diseases) where deposition of misfolded insoluble protein aggregates within cells of the central nervous system renders the protein dysfunctional.
Recently, the appearance in 1986 in great Brittan of the mad cow disease epidemic followed by the new variant of Creuztfeldt-Jacob disease in man was due to ingestion of infected cattle meet or by blood transfusion. These disorders are due to an infectious agent called prion protein (PrPsc) originates through conformational changes of the normal cellular prion protein (PrPc).
Adding streptomycin to proteinase K treated PrPsc showed an increase of the molecular mass of the 3 bands proportional to the quantity to the added streptomycin. It induced flocculation and aggregation of the PrPscwhich can easily separate by low centrifugation step. The mechanism of interaction occurred through hydrogen bond transfer between the 2 guanidine groups present on streptomycin and the amino-acids of one or several prion proteins ruled the possibility of a schiff-base reaction.
The inoculation intraperitonially of a mixture of streptomycin and infected mouse brain homogenate of the scrapie strain C506M3 into C57BL6 mice showed 45 days post inoculation a decrease to even absence of accumulated prion infectivity marker PrrPsc in the mice spleen in comparison to controls. This result can be explained by changes of the surface electric charges due to the hydrogen bond transfer between the two guanidine groups present on streptomycin and the different amino acids on the PrrPscpeptides and thus probably affecting the stability and induced a drop in the PrrPsc infectivity. These results probably show that proteinase K resistance of the PrrPsc is not associated with infectivity.
Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Recommended Conferences

Article Usage

  • Total views: 11426
  • [From(publication date):
    specialissue-2014 - Jun 28, 2017]
  • Breakdown by view type
  • HTML page views : 7675
  • PDF downloads :3751
 

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords