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Concentration Analysis of Rapid-Equilibrium Binding between Warfarin and Human Serum Albumin Using a Gradient Surface Plasmon Resonance Technique | OMICS International
ISSN: 2168-9431
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Concentration Analysis of Rapid-Equilibrium Binding between Warfarin and Human Serum Albumin Using a Gradient Surface Plasmon Resonance Technique

Preejith Vachali1* and Aaron Martin2
1University of Utah, Moran Eye Center, 65 Mario Capecchi Dr, Salt Lake City, USA
2SensíQ Technologies, 800 Research Parkway, Oklahoma City, USA
Corresponding Author : Preejith Vachali
University of Utah, Moran Eye Center
65 Mario Capecchi Dr, Salt Lake City, USA
Tel: +1 801-581-2352
E-mail: [email protected]
Received March 03, 2015; Accepted March 04, 2015; Published March 06, 2015
Citation: Vachali P and Martin A (2015) Concentration Analysis of Rapid-Equilibrium Binding between Warfarin and Human Serum Albumin Using a Gradient Surface Plasmon Resonance Technique . Single Cell Biol 4:i103. doi: 10.4172/2168-9431.1000i103
Copyright: ©2015 Vachali P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Clinical Image
image
Warfarin binding immobilized HSA (left panel) using the sigmoidal gradient (OneStep®) on a SensíQ Pioneer SPR system. Warfarin sample (500 μM top concentration) was injected in triplicate. Concentration view (right panel) shows the signal transformed versus analyte concentration with error bars showing standard deviation of SPR replicates. Analysis of response versus concentration yields interaction affinity when binding is at equilibrium.
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