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ISSN: 2329-6798
Modern Chemistry & Applications
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Degradation of Vancomycin Hydrochloride by Electrooxidation

Matheus HA1, Daniel RA1* and Wilson AC2

1Department of Environmental Sciences, Southern Brazilian Federal Institute for Education, Science and Technology (IFSul), Pelotas, Brazil

2Federal University of Pelotas, Pelotas, Brazil

*Corresponding Author:
Daniel Ricardo Arsand
Teacher, Department of Environmental Sciences Southern
Brazilian Federal Institute for Education Science
and Technology (IFSul), Pelotas, Brazil
Tel: 5553984660120
E-mail: [email protected]

Received date: August 11, 2017; Accepted date: August 19, 2017; Published date: August 23, 2017

Citation: Matheus HA, Daniel RA, Wilson AC (2017) Degradation of Vancomycin Hydrochloride by Electrooxidation. Mod Chem Appl 5:230. doi: 10.4172/2329-6798.1000230

Copyright: © 2017 Matheus HA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Many pharmaceuticals are considered recalcitrant pollutants and represent a problem for human and animal health due to continuous contribution in the aquatic environment and the bacteria resistance development to antibiotics. Vancomycin is one of the most antibiotics administered in medicine, nevertheless, there is not much knowledge about the presence, fate and the effects of Vancomycin in the environment. The advanced oxidation techniques (AOTs) are shown as alternatives for the treatment of water and wastewater in order to degrade pollutants and contaminants. Therefore, this work is aimed to evaluate the Vancomycin degradation in an aqueous medium using electrooxidation (EO) technique and to optimize the reaction conditions. The experiments were conducted in a homemade electrochemical cell in acrylic with a working volume of 500 cm3; DSA electrodes – Dimensionally Stable Anodes (70TiO2-30RuO2) were used (effective area of work - 41.25 cm2); NaCl P.A. was used as supporting electrolyte; 130 mg L-1 of Vancomycin was
used in aqueous solution. The optimization was done using central composite design (CCD) totaling 18 experiments. The evaluated factors were: interelectrodes distance (ID), applied current and supporting electrolyte concentration. All experiments were conducted for 20 minutes with sampling at 0, 1, 2, 3, 4, 5, 10 and 20 minutes. Vancomycin concentrations were determined by High Performance Liquid Cromatography with Diode-Array Detection (HPLC-DAD) (Mobile phase: phosphate buffer 0.05 mol L-1, pH 4.7: MeOH: ACN [80:15:5, v/v], Injection volume: 40 L; C18 with flow rate of 1.0 mL min-1, λ: 210 nm. The results show that the electrooxidation is effective in Vancomycin degradation, showing as an alternative to degradation of this drug. The method used obtained 100% of degradation in 2 minutes of treatment in optimum conditions: 400 mA, 3 cm interelectrodes distance and 1,100 mg L-1 NaCl.

Keywords

Pharmaceutical drugs; Antibiotics; Electrochemical; Wastewater treatment

Introduction

In the 1970s, the scientific community initiated research on the presence of drugs in the environment and the possible impacts resulting from its perennial use, continuous intake and low concentrations [1]. Because of the presence of these substances and its byproducts in the environment, they are considered as a problem of great magnitude in many countries [2]. The introduction of drugs into the environment is mainly due to the disposal of urban sewage without being treated and due to human and animal excretion. However, soil fertilization with manure and effluents from the pharmaceutical industry cannot and should not be disregarded. Antidepressants [3], anti-inflammatories [4], contraceptives [5] and antibiotics [6] are found in wastewater treatment plants (WWTPs), surface waters and groundwater with concentrations in the ng and μg L-1 bands. Antibiotics occupy a prominent place among the classes of drugs spread in human and animal medicine. Kümmerer [7] considers antibiotics substances require special attention because they have different molecular structures along with diverse physical, chemical and biological characteristics and also because they behave in different ways with pH variation, such as: neutral, cationic, anionic or zwitterionic. Antibiotic resistance genes were found to many antibiotics, including Vancomym by Luprano [8] and Çardak [9]. Antibiotics are subdivided into several groups: Quinolones, β-Lactams, Tetracyclines, Macrolides and Glycopeptides. The class of glycopeptides has Vancomycin (Figure 1) as its main representative with high molecular mass, used in hospitals intravenously and mainly in infections caused by S. aureus and Enterococcus spp, where the microorganisms present some or total resistance to treatments or when the patient is allergic to penicillin. In addition, the microorganisms present resistance to Vancomycin itself, which is considered an antimicrobial (as a reference) in the treatment of such infections [10,11]. Searching for ways to contain the supply of antibiotics in the environment, numerous studies aim to develop and explore new types and/or new effluent treatment technologies, since the most widespread biological treatments achieve efficiencies of about 50% in the biodegradation of drugs in systems of activated sludge reactor; physical treatments only promote the transfer of drugs; and traditional chemical processes often do not promote complete mineralization of pharmaceuticals, ultimately by making available byproducts of them [1]. Qiu [12] studied Vancomycin under two typical pharmaceutical wastewater treatment plants and, despite the removal obtained, the results indicated the non-negligible environmental and health risks to Vancomycin. Advanced Oxidation Techniques (AOTs) have been shown as an efficient alternative in drug degradation. Lüddeke [13] have experimented with the efficiency of the ozonation process in various combinations with sand and charcoal filters in order to optimize the treatment totally for antibiotic resistant bacteria. The photo-peroxidation [14], Fenton [15] and Photo-fenton [16] are examples of AOTs used in water and effluent treatment. Following the line of the new treatment process, the electrooxidation has been used, also called as anodic oxidation when promotes the oxidation of molecules adsorbed on the surface of the inert electrode [17,18]. There is not much knowledge about the presence, fate and the effects of Vancomycin in the environment. This way, the objective of this work was to study the degradation of Vancomycin in an aqueous medium using electrooxidation technique and to optimize the process to promote the degradation of Vancomycin and its possible degradation products.

modern-chemistry-applications-Molecular-structure

Figure 1: Molecular structure of Vancomycin.

Materials and Methods

Experimental optimization

The experiments were optimized using experimental planning through the central composite design model (CCD), totaling 18 experiments: 8 factorial, 4 central and 6 axial. The three variable factors evaluated were: interelectrode distance (2 to 8 cm), applied current (133 to 467 mA), and the concentration of support electrolyte (566 to 1234 mg L-1 NaCl), together with 5 levels of significance (-1.67, -1, 0, +1 e +1.67). Table 1 shows the evaluated factors and the matrix of the experiments. The analysis of variance (ANOVA) was used, considering the statistical significance of 95% (p<0.05), to evaluate the significant parameters. The experiments were carried out at random to ensure the results would be reliable. (C) – Central point, control experiment.

Experiment Factors (Real values)
Current (mA) Interelectrodes Distance (cm) Support Electrolyte (mg L-1)
1 -1.00 (200) -1.00 (3) -1.00 (700)
2 -1.00 (200) 1.00 (7) 1.00 (1100)
3 1.00 (400) -1.00 (3) 1.00 (1100)
4 1.00 (400) 1.00 (7) -1.00 (700)
5 (C) 0.00 (300) 0.00 (5) 0.00 (900)
6 -1.00 (200) -1.00 (3) 1.00 (1100)
7 -1.00 (200) 1.00 (7) -1.00 (700)
8 1.00 (400) -1.00 (3) -1.00 (700)
9 1.00 (400) 1.00 (7) 1.00 (1100)
10 (C) 0.00 (300) 0.00 (5) 0.00 (900)
11 -1.67 (133) 0.00 (5) 0.00 (900)
12 1.67 (467) 0.00 (5) 0.00 (900)
13 0.00 (300) -1.67 (2) 0.00 (900)
14 0.00 (300) 1.67 (8) 0.00 (900)
15 0.00 (300) 0.00 (5) -1.67 (566)
16 0.00 (300) 0.00 (5) 1.67 (1234)
17 (C) 0.00 (300) 0.00 (5) 0.00 (900)
18 (C) 0.00 (300) 0.00 (5) 0.00 (900)

Table 1: Experiments and variable factors used in electrooxidation experiments.

Analytical procedure

Vancomycin concentrations were determined using a high performance liquid chromatograph with a diode arrangement detector (HPLC-DAD), a S3240 UV/VIS multi-channel detector and a S1125 pump system, both from Sykam, adapted according to Santos [19]. Mobile phase was composed of 0.05 mol L-1 phosphate buffer, pH 4.7:methanol:acetonitrile [80:15:5, v/v]. The injection volume was 40 μL; a Dr. Maisch C18 reverse phase column (250 mm × 4.6 mm internal diameter) containing 5 μm particles was used; the flow rate used was 1.0 mL min-1, in a gradient elution system. Vancomycin was determined using the wavelength of 210 nm as reference. The analytical curve was constructed from five concentration points (200, 100, 25, and 12 mg L-1) in triplicate (y=60.229+193.33; R2: 0.9982).

Electrooxidation experiments

The experiments were carried out in homemade electrochemical cells in acrylic with magnetic stirring; working volume of 500 cm3, DSA® electrodes were used as anode and cathode (Figure 2) based on titanium oxide and ruthenium oxide in the ratio (70TiO2-30RuO2) provided by De Nora (Brazil). The working current was applied using a Polimed® PMI 3002 S current source. Each electrode presented an effective working area of 41.25 cm2. The Vancomycin in all experiments was about 130 mg L-1 in deionized water (prepared in the laboratory). NaCl P.A. was used as supporting electrolyte. The experiments lasted for 20 minutes and samples were collected at 0, 1, 2, 3, 4, 5, 10 and 20 minutes and stored in the absence of light at 4 ± 1°C until analytical procedures.

modern-chemistry-applications-Electrochemical-system

Figure 2: Electrochemical system for EO experiments: 1) current supply; 2) cathode DSA; 3) anode DSA; 4) electrochemical reactor; 5) magnetic stirrer.

Results and Discussion

Vancomycin electrooxidation and optimization of the reaction parameters

The results obtained in the EO experiments of Vancomycin are presented in Table 2. It can be seen that at 3 minutes of electrooxidation, four experiments reached 100% of degradation of Vancomycin hydrochloride. However, this time is not adequate for optimization of parameters, as it does not allow evaluation of the best efficiency related to the reaction conditions. Therefore, the degradations obtained in 2 minutes for the optimization of the reaction conditions were evaluated.

Experiment (n) 1st minute of degradation (%) 2nd minute of degradation (%) 3rd minute of degradation (%)
1 43.1 59.2 68.7
2 39.2 63.3 79.0
3 86.2 100.0 100.0
4 39.0 73.3 100.0
5 (c) 40.2 78.5 79.9
6 45.6 63.2 63.6
7 31.4 48.3 58.0
8 58.0 70.4 100.0
9 56.4 86.9 91.4
10 (c) 58.8 76.3 100.0
11 28.4 35.8 44.6
12 0.0 85.8 98.8
13 51.4 70.3 83.0
14 40.0 61.8 74.2
15 30.3 55.5 70.0
16 64.6 82.0 98.9
17 (c) 49.4 70.6 92.3
18 (c) 51.0 69.5 86.9

Table 2: Vancomycin degradation at the electrooxidation experiments.

Optimization of reactional conditions

The mathematical model used to evaluate the influence of analyzed parameters on Vancomycin EO, considering the CCD model, is presented in Equation 1.

Y=bo+b1.x1+b11.x12+b3.x3+b33.x32+b22.x22+b2.x2+x1.x2.b123 Eq. 1

The analysis of variance (ANOVA) of the results obtained is presented in Table 3, considering statistical significance of 95% (p<0.05). The results showed that 90.44% (R2) of the degradation is explained by the proposed value. Thus, it can be observed that the concentration of support electrolyte used and the current applied are significant in the electrooxidation of Vancomycin, whereas the interelectrode distance, under the tested conditions, is not Martínez-Huitle [20] carried out the effluent electrooxidation of the textile industry by varying the applied current and obtained better results, even in shorter experiment times by applying higher electric currents. In the same sense, Singh [21] obtained better indices of dye degradation by applying higher currents using DSA electrodes. Malpass [22] obtained 100% degradation as a result, after 1 h of electrooxidation by being efficient in a similar configuration (DSA electrodes and NaCl as support electrolyte), in the oxidation of pesticide atrazine.

  Estimated Standard error T Value p Value
X1 13.26430 1.641074 8.08269 0.000011
X2 -3.29439 1.716838 -1.91887 0.083965
X3 7.84037 1.641074 4.77759 0.000748

Table 3: T and Pvalues, standard error and estimated values for the parameters: applied current “X1”; interelectrodes distance “X2”; and concentration of support electrolyte “X3”.

The degradation of Vancomycin, under these conditions, resulted in the quadratic mathematical model (Equation 2):

D%=7.3611+0.177x+0.0009y-0.0003x2+0.0002xy-3.9473 10-6y2 Eq. 2

where, “D%” is Vancomycin degradation [%], “x” is applied current [mA] and “y” is support electrolyte concentration [mg L-1].

The response surface resulting from this process is shown in Figure 3 which allows the visualization of the behavior of response (degradation) in relation to the different values of the electrolyte and the applied electric current. The points marked on the surface represent Experiment 3 (I: 400 mA and NaCl 1100 mg L-1) as the best result whereas experiment 11 (I: 133 mA and NaCl 900 mg L-1) reached the lowest percentage of degradation. It can be seen that the degradation of Vancomycin is more pronounced in conditions where higher currents are applied and higher concentrations of support electrolyte are used. This behavior is attributed to the sum of direct and indirect oxidation processes, since better results are obtained in the presence of chloride ions due to the indirect oxidation resulting from the formation of molecular chlorine in the medium [23] or even resulting from the formation of hydroxyl radicals (HO·) [21]. Thus, the higher Vancomycin degradation efficiency of experiment 3 is attributed to the promotion of molecular chlorine generation (Cl2) due to the higher concentration of support electrolyte used.

modern-chemistry-applications-Vancomycin-hydrochloride

Figure 3: Response surface for degradation of Vancomycin hydrochloride in function of variation of electrolyte concentration and the applied electric current.

Evaluation of the resulting model and the results obtained

The results obtained show the configuration of experiment 3 as a better degradation performance. Experiments 4, 8 and 10 also reached 100% degradation, however, with longer applied current. Comparing the reaction conditions, it is possible to see the use of higher electrolyte support in experiment 3. These results confirm the mathematical model proposed and the response surface. Experiments 6, 8 and 9 (Figure 4) show the change of a single factor in relation to experiment 3 (I: 400 mA, 1100 mg L-1 NaCl and ID: 3 cm): applied current (200 mA) in experiment 6; Concentration of the supporting electrolyte (700 mg L-1) in experiment 8; and interelectrode distance (7 cm) in experiment 9. Experiment 6 explains the dependence between the degradation of Vancomycin hydrochloride and the applied current; experiment 9 shows that the interelectrode distance also influences the performance of electrooxidation, however, with less interference in the oxidation; experiment 8 shows a lower electrolyte concentration and a condition in which 100% degradation is achieved.

modern-chemistry-applications-oxidation-times

Figure 4: Comparative between oxidation times and the respective degradation of Vancomycin hydrochloride.

Degradation products and vancomycin mineralization

Although Vancomycin is completely degraded in the first few minutes of the experiment, it can be seen as byproducts being generated from the mother molecule. Figures 5 and 6 shows the formation of other substance(s) during the course of experiment 2, which was chosen to allow better visualization of Vancomycin signal decay and increase of signals between 11 and 14 minutes of chromatographic reading. These experiments were done until 20 minutes to observe the byproducts formation/degradation.

modern-chemistry-applications-minutes-oxidation

Figure 5: Chromatogram of experiment 2, samples between one to five minutes of oxidation.

modern-chemistry-applications-minutes-electrooxidation

Figure 6: Chromatograms between 1 and 5 minutes of electrooxidation (a); 10 and 20 minutes of experiment 11 (b).

Conclusion

The results showed that the EO technique was efficient in the degradation of Vancomycin hydrochloride (HVCM), under the conditions using DSA® electrodes (70TiO2-30RuO2). Furthermore, it can be stated that the best conditions for the degradation of Vancomycin in aqueous medium were 400 mA of applied current, 3 cm of interelectrode distance and 1100 mg L-1 of support electrolyte [NaCl]. The electrical current is the main factor responsible for affecting the degradation of Vancomycin hydrochloride, but the use of sodium chloride as a support electrolyte and the generation of Cl2 during the oxidation process must be an attributed part of this degradation.

Acknowledgements

The authors gratefully acknowledge Capes Foundation (Brazilian Ministry of Education), Council for Scientific and Technological Development (CNPq) and the Federal Institute Sul-rio-grandense do Sul campus Pelotas for supporting the study.

References

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