Received Date: October 30, 2014; Accepted Date: October 31, 2014; Published Date: November 03, 2014
Citation: Rijkers GT, Meek B and Jong BD (2014) Determination of Pneumococcal Antibodies in Serum with Multiplex Technology . J Mol Biomark Diagn 5:I102. doi:10.4172/2155-9929.1000I102
Copyright: ©2013 Rijkers GT, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Determination of pneumococcal antibodies in serum with multiplex technology. Streptococcus pneumoniae, the primary causative agent of pneumonia and otitis media, occurs in 92 different serotypes. The serotype is defined by the structure of the capsular polysaccharide.
In the Laboratory of Medicial Microbology and Immunology of the St. Antonius Hospital in Nieuwegein, The Netherlands, we have developed a multiplex assay, enabling the simultaneous measurement of antibodies against the 25 most prevalent pneumococcal serotypes. The assay reuires only small amounts of blood (50 microliter) and therefore can be used for all patients, including babies. Shown is a screen-shot of an analysis using Luminex software, indicating the positioning of the 25 gates for the beads coupled with 25 different serotypes.
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