alexa Efficient Direct Electron Transfer for a Highly Oriented PQQ-GDH Immobilized Electrode for Bioanode | OMICS International
ISSN: 2155-6210
Journal of Biosensors & Bioelectronics
Like us on:
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Efficient Direct Electron Transfer for a Highly Oriented PQQ-GDH Immobilized Electrode for Bioanode

Ayako Koto1, Saki Taniya2, Hiroaki Sakamoto1*, Takenori Satomura2, Haruhiko Sakuraba3, Toshihisa Ohshima4 and Shin-ichiro Suye1,2

1 Department of Frontier Fiber Technology and Science, Graduate School of Engineering, University of Fukui, Fukui, Japan

2 Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Fukui, Japan

3 Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Kanagawa, Japan

4 Department of Biomedical Engineering, Faculty of Engineering, Osaka Institute of Technology, Osaka, Japan

*Corresponding Author:
Hiroaki Sakamoto
Department of Frontier Fiber Technology and Science
Graduate School of Engineering, University of Fukui
Fukui, Japan
Tel: 0776-27-9753
E-mail: [email protected]

Received Date: January 29, 2014; Accepted Date: March 27, 2014; Published Date: April 02, 2014

Citation: Koto A, Taniya S, Sakamoto H, Satomura T, Sakuraba H, et al. (2014) Efficient Direct Electron Transfer for a Highly Oriented PQQ-GDH Immobilized Electrode for Bioanode. J Biosens Bioelectron 5:148. doi: 10.4172/2155-6210.1000148

Copyright: © 2014 Koto A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Journal of Biosensors & Bioelectronics


A bioanode with improved enzyme orientation was developed to achieve an efficient enzyme reaction and electron transfer on an electrode surface. A highly stable PQQ-dependent glucose dehydrogenase (PQQ-GDH) isolated from a hyper-thermophilic archaeon was employed as an electron conversion element. PQQ-GDH is expected to maintain battery properties and to have a long battery life. To immobilize the enzyme onto the electrode with appropriate orientation, we introduced a His-tag to the N-terminal of PQQ-GDH by a genetic technique and utilized the affinity bond between His-tag and Cu atoms. The catalytic current density in the presence of substrate was 18.6 μA/cm2 without a mediator. The current density of the oriented electrode was approximately 90 times higher than that of the non-oriented electrode. By immobilizing the enzyme with orientation, the accessibility between the enzyme and substrate for enzyme reaction increased because the active site of PQQ-GDH is located opposite the electrode. Because enzymes have different orientations at the surface of the non-orientated electrode, the efficiency of the electrode was lower than that of the high-orientation electrode. The results of the present study present a potentially promising finding for application to practical bioelectric devices, such as bio-fuel cells and biosensors.


Bioanode; PQQ-GDH; Oriented immobilization; Direct electron transfer


Bio-fuel cells catalyzed by enzymes are expected to provide next-generation energy supply sources for microelectronic devices because they are safe to use and are light in weight and can be easily miniaturized [1-4]. The principle of bio-fuel cells is based on the redox reaction between the enzyme and substrate. The substrates are prepared from biomass and include glucose, methanol, ethanol, and lactate. Previously, many researchers have developed enzymatic biofuel cells [5-13] that were shown to have current and power densities to some extent, but were limited by major issues including low output of the battery because of inefficient redox reaction on the electrode surface and lower active lifetime because of low stability of enzyme [14,15]. Therefore, it has been considered difficult to achieve a practical bio-fuel cell thus far.

In the present study, in order to overcome the above issues, the following two strategies were used.

The first strategy was to increase the orientation of the enzyme immobilized onto the electrode surface. Because electron-transfer between the enzyme and electrode, and enzyme reactivity on the electrode surface, depend on the orientation of enzyme [16-19], controlled and oriented immobilization of enzyme onto the electrode surface is required [20-25]. However, it is not easy to modulate macromolecules, e.g., enzymes and peptides, on the electrode surface. Because macromolecules have an intricate chemical and physical structure, the enzyme orientation on the electrode surface is the major rate-limiting factor for enzyme function. In the past, various approaches, such as covalent cross-linking [26,27], Layer-By-Layer (LBL) [28-30], and Self-Assembled Monolayers (SAM) [31-34], have been reported for the immobilization of macromolecules on solid substrates. Almost all of these techniques have been based on covalent cross-linking through chemical reactions that lack site selectivity. Using the above immobilization methods, specificity of the reaction site in the macromolecule cannot be obtained because the macromolecules have more than one reactive group on their surface.

The second approach is to employ PQQ-GDH obtained from the hyperthermophilic archaeon, Pyrobaculum aerophilum [35]. PQQ-GDH from hyperthermophiles, which can grow at or near the boiling point of water, is much more stable than that obtained from mesophilic microorganisms or eukaryotic organisms. In addition, PQQ-GDH can utilize artificial dyes such as 2,6-Dichloroindophenol (DCIP) and ferricyanide as electron acceptors. PQQ-GDH has high potential for utilization as a specific element for a bioanode electrode because electrons from D-glucose can be introduced to the electrode using an artificial dye as the mediator. Use of PQQ-GDH from the hyperthermophilic archaeon provided a long cell life and high power output.

In the present study, we developed a bio-anode electrode by highly oriented immobilization of PQQ-GDH. To immobilize the enzyme with orientation, we employed the interaction between Histag (consisting of six successive histidine residues) and the Cu atom. As the His-tag acts as an immobilization site on the electrode surface, the enzyme can be immobilized with a high degree of orientation (Figure 1). We have introduced the His-tag into the PQQ-GDH from Pyrobaculum aerophilium by using a genetic technique. The PQQGDH-( His-tag) was immobilized onto a Cu-deposited electrode with a high degree of orientation. The highly oriented immobilized PQQGDH electrodes were investigated using electrochemical techniques to obtain information on the properties of the constructed electrode.


Figure 1: Schematic illustration of PQQ-GDH-(His-tag) immobilization onto the Cu deposited electrode surface. Deposited Cu (orange spheres) and His-tag (green arcs) are forming affinity binding.

Materials and Methods


D(+)-Glucose, sodium chloride, copper(II) sulfate pentahydrate (CuSO4 ·5H2O), nickel(II) sulfate hexahydrate (NiSO4 ·6H2O), imidazole, 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Hydrochloride (EDC) and Pyrroloquinoline quinone disodium salt (PQQ) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Potassium chloride (KCl) and 2-[4-(2-Hydroxyethyl) piperazin-1-yl] ethanesulfonic acid (HEPES) were obtained from Nacalai Tesque. Inc. (Kyoto, Japan). 2-Morpholinoethanesulfonic acid, monohydrate (MES) was purchased from Dojindo Laboratories (Kumamoto, Japan). The water used in the present experiment was first deionized and passed through a Milli-Q water purification system from Millipore Co. (Bedford, MA, USA).

Preparation of PQQ-GDH-(His-tag)

To prepare purified PQQ-GDH-(His-tag) by nickel affinity chromatography, a six-His sequence was fused at the N-terminal of the PQQ-GDH protein. The pET15b/PaeAsd plasmid encoding PQQ-GDH was used as the PQQ-GDH-(His-tag) expression vector. The expression vector of the PQQ-GDH-(His-tag) fusion protein was constructed as follows. The coding region of the PQQ-GDH gene was obtained by digesting pET/PaeAsd with NdeI/BamHI [36], and then the fragment was ligated with the expression vector pET15b (Novagen, Merck Millipore Japan (Tokyo, Japan)), which had been linearized with NdeI and BamHI. The pET15/PaeAsd plasmid was verified by DNA sequencing.

PQQ-GDH-(His-tag) was expressed in Escherichia coli BL21- CodonPlus(DE3)-RIL grown in LB medium containing ampicillin. Recombinant PQQ-GDH-(His-tag) proteins were induced by adding 1 mM isopropyl-β-D-thiogalacto-pyranoside at OD600 0.6, and cultivation was continued for an additional 4 h. Cells were harvested by centrifugation at 10,000×g, suspended in lysis buffer (20 mM HEPES (pH 7.5), 100 mM NaCl, 1 mM CaCl2), and disrupted by ultrasonication. The cell debris was removed by centrifugation (12,000×g for 10 min) and the supernatant solution was used as the crude extract. The enzyme solution was incubated at 80°C for 10 min. Denatured proteins were separated by centrifugation (12,000×g for 10 min).The resultant supernatant was used for further purification. To reconstitute the apoenzyme with PQQ, a 10-fold molar excess of PQQ was added into the enzyme solution and incubated it for 16 h at 4°C.

To obtain purified PQQ-GDH-(His-tag), the holoenzyme, after reconstitution of the apoenzyme with PQQ, was purified by using Ni- NTA chromatography (HisTrap HP column; GE Healthcare UK Ltd, Little Chalfont, Bucks, UK). The enzyme was loaded onto the column equilibrated with 20 mM potassium phosphate buffer at pH 7.5 and eluted from the column using an elution buffer (300 mM NaCl, 500 mM imidazole, 20 mM potassium phosphate buffer, at pH 7.0). The purified PQQ-GDH-(His-tag) proteins were dialyzed to remove imidazole and replace the buffer (50 mM HEPES pH 7.5), and stored at room temperature until further use.

The activity of PQQ-GDH-(His-tag) was measured using 2,6-Dichlorophenolindophenol (DCIP) as described previously [37].

Electrochemical experiments

Cyclic Voltammetry (CV) was conducted using a three-electrode cell at 50°C on an ALS electrochemical analyzer (BAS Inc., Tokyo, Japan). The working electrode was gold (geometrical area: 0.02 cm2, BAS Inc.), the counter electrode was a platinum wire, and the reference electrode was Ag/AgCl. All potential values below are reported with respect to the Ag/AgCl reference electrode. The electrolyte solution was 50 mM HEPES buffer at pH 7.5. The CV measurements were recorded at a potential scan rate of 0.01 V/s. The working electrode was sonicated in 50 mM H2SO4, and then the surface was polished with a micro-cloth and alumina slurry suspension (φ=0.1 μm). The electrode was scanned 50 times at a potential scan rate of 0.1 V/s, at potentials ranging from -1.0 V to 1.0 V. Finally, the electrode was sonicated in Milli-Q water.

Electrode modification

Au/Cu/ PQQ-GDH-(His-tag): CuSO4 was dissolved in 100 mM sodium chloride to a final concentration of 100 mM. Copper deposition was controlled by applying a potential of -0.3 V vs. Ag/AgCl to the Au electrode for 5 min. Then, the electrode was rinsed with Milli-Q water and immersed in 0.98 mg/ml PQQ-GDH-(His-tag) solution (at room temperature, 5 min) to immobilize the PQQ-GDH-(His-tag). The electrode was rinsed again with 50mM HEPES buffer at pH 7.5.

C/ PQQ-GDH-(His-tag): The freshly polished carbon electrode (0.07 cm2) was immersed in 100 mM H2SO4 for 1 h, washed, and electrolytically oxidized at +1.2 V in 5% K2Cr2O7 in 10% HNO3 to introduce carboxyl groups on the electrode surface. The electrode was immersed in freshly prepared 400 mM EDC solution in 10 mM MES buffer at pH 4.5 for 1 h, and then immersed in 0.98 mg/ml enzyme solution overnight at room temperature.

Estimation of electrode area

CV was conducted with the Au/Cu electrode and the carbon electrode in the 25 mM potassium ferricyanide/25 mM potassium ferrocyanide solution. Potassium ferrocyanide and potassium ferricyanide react as shown in Eq. (1). The peak reflecting the number of molecules that have reacted at the electrode surface obtained from CV and the electrode area (A) was calculated using the peak and Eq. (2). Ip is the peak current, n is the number of electrons, DR is the diffusion coefficient, ν is the scan rate, and C is the concentration.



Quantification of the immobilized enzyme

To remove the enzyme from electrode surface, the Au/Cu/ PQQPQQ GDH-(His-tag) electrode was immersed in the 500 mM imidazole solution (0.2 ml) for 30 min at room temperature. Quantification was performed using this solution. We determined the amount of immobilized His-PQQGDH using a protein quantification kit (InvitrogenTM CBQCA Protein Quantitation Kit (C-6667); Life Technologies Co., Carlsbad, CA, USA) and followed the kit instructions. Bovine serum albumin was used as the standard protein.

Results and Discussion

Long-term Stability of PQQ-GDH-(His-tag)

It is known that PQQ-GDH-(His-tag) from the hyperthermophilic archaeon P. aerophilum is much more stable than PQQ-GDH from mesophilic organisms under a variety of conditions such as a wide range of pH values and temperatures [38]. For this enzyme, smaller overall volumes have been shown to contribute to greater protein thermostability. Aromatic interactions are also known to participate in the stabilization of enzyme structures. When used as an electrode for a bio-fuel cell, long-term stability is also an important property. Assuming storage at room temperature, we examined the longterm stability of the enzyme. The PQQ-GDH-(His-tag) solution was stored at room temperature, and the activity was measured every few days. Enzyme activity was measured using DCIP, and even after approximately one month, the enzymatic activity of PQQ-GDH-(Histag) was maintained at more than 90% (Figure 2).


Figure 2: Long-term stability of PQQ-GDH-(His-tag) from hyperthermophilicarchaeon (n=3). Residual activity of the PQQ-GDH-(His-tag) (open circles) was measured after storage at room temperature.

Electrocatalytic reaction of immobilized PQQ-GDH-(Histag)

PQQ-GDH-(His-tag) catalyzes the oxidation of D-glucose. Direct Electron Transfer (DET) is achieved by passing of electrons produced by oxidation of D-glucose to the electrode through the PQQ existing inside the enzyme. To immobilize the enzyme with orientation, the affinity between the His-tag and deposited Cu atoms was utilized. The His-tag domain is widely used for protein purification and for molecular immobilization using nickel-nitrilotriacetic acid (Ni-NTA) [39,40] because it can form a complex with transition metal ions. It was recently reported that not only transition metal ions, such as Ni2+, Co2+, Cu2+, Zn2+, but also zero-valent metals can form complexes [41]. Using this reaction, we can immobilize enzymes with a high degree of orientation via a His-tag, without requiring modification involving NTA. Therefore, we prepared the PQQ-GDH-(His-tag) modified electrode on the deposited Cu electrode (Au/Cu/PQQ-GDH-(Histag)). Figure 3 shows the CV of the catalytic reaction of the Au/Cu/ PQQ-GDH-(His-tag) electrode. Because deposited Cu atoms are not oxidized at less than 0 V, CV was performed from -0.3 V to 0 V. When using the Au/Cu/PQQ-GDH-(His-tag) in the absence of substrate, a small peak was observed at -0.11 V, which represents oxidation of PQQ in the enzyme by DET, which confirmed DET between the enzyme and electrode, as previously reported [36-38]. When 5 mM D-glucose existed in the electrolyte solution, the catalytic current arising from the oxidation reaction catalyzed by PQQ-GDH-(His-tag) was observed in the vicinity of -0.1 V vs. Ag/AgCl. When PQQ was not incorporated in the GDH and enzymes were not immobilized on the electrode, a similar peak was not observed, even after addition of D-glucose. These findings prove that a catalytic oxidation current was obtained from the reaction of immobilized enzymes on the Au/Cu electrode.


Figure 3: Typical cyclic voltammogram of the Au/Cu/ PQQ-GDH-(His-tag) electrode in the absence (dotted-line) and presence of 5 mM D(+)-Glucose (solid line) in 50 mM HEPES-NaOH buffer (pH 7.5) at a scan rate of 0.01 V/s.

Confirmation of highly oriented immobilization

We have confirmed that the PQQ-GDH-(His-tag) is immobilized to the Au/Cu electrode via the His-tag. His-tag is a short peptide chain consisting of six histidine residues that coordinates to the transition metal by sharing the electron density of the nitrogen of the imidazole ring of histidine in the His-tag. Upon imidazole treatment, imidazole molecules act antagonistically to the imidazole ring of histidine residues of His-tag; then, the His-tag leaves the Cu, and the protein is removed from the electrode surface. In the CV measurements using electrodes treated with imidazole, the peak value of the catalytic current of oxidation of D-glucose was dramatically decreased (Figure 4). With an increase in the concentration of imidazole, reduction of the peak value was also observed. Because PQQ-GDH-(His-tag) is definitely eliminated by imidazole treatment, the enzyme definitely binds to the electrode via Cu atoms. Therefore, the immobilized enzymes may achieve a high degree of orientation on the electrode.


Figure 4: Typical cyclic voltammogram of PQQ-GDH-(His-tag)/Cu/Au electrode in the presence of 5 mM D(+)-Glucose without treatment involving imidazole solution (dotted-line) and with treatment involving imidazole solution (solid line), in 50 mM HEPES-NaOH buffer (pH 7.5) at a scan rate of 0.01 V/s.

Effect of the high degree of orientation of the enzyme

We compared the efficiency of a highly oriented electrode containing a His-tag (Au/Cu/PQQ-GDH-(His-tag)) and a nonoriented electrode functionalized by EDC (C/PQQ-GDH-(His-tag)). To evaluate the efficiency of enzyme reaction on the electrode surface, the properties of the electrode (electrode area and total amount of electroactive coverage on electrodes) were analyzed.

The electrode area of the Cu-deposited Au electrode (Au/Cu) was determined using the electrode reaction of potassium ferricyanide. The value of -0.925 mA was obtained for the reduction peak; thus, the electrode area was estimated to be 0.40 cm2 by using equation (2). Before deposition, the geometric area of the Au electrode was 0.02 cm2, so the electrode area was increased by 20-fold compared with the bare Au electrode.

To determine the total amount of immobilized enzyme on the electrode, we modified gold electrodes as described above and then washed off the His-tagged enzymes by immersing the electrode in an imidazole solution. After washing, the total amount of protein in the imidazole solution was measured. The protein concentration was determined according to the BSA calibration curve. PQQ-GDH- (His-tag) attached to the modified gold electrode with a density of 5.25×10-11 mol/cm2. However, it was not possible to determine the amount of immobilized enzyme on the randomly oriented electrode because of the lack of an effective method.

CV was also used to study the DET electrochemistry of PQQGDH-( His-tag) immobilized on the Au/Cu electrodes. PQQ-GDH- (His-tag) shows DET properties derived from the redox of PQQ in CV measurements (Figure 3). In this study, the His-Cu affinity bond and EDC amide bond were used for enzyme modification on the Au/Cu/ PQQ-GDH-(His-tag) electrode and C/PQQ-GDH-(His-tag) electrode, and electroactive coverage of each electrode was estimated from the oxidation peak current of PQQ (IpPQQ). The peak current for an electrode-immobilized reactant is given by Eq. (3).


where n is the number of electrons transferred (2), F is the Faraday constant, R is the universal gas constant, T is the absolute temperature in Kelvin (313.15 K), A is the surface area of the electrode, Γ is the surface coverage, and ν is scan rate (0.01 V/s). From IpPQQ (0.73× 10-6 A (Figure 4 gray line)), the estimated number of molecules of the electroactive enzyme immobilized on the electrode surface (ΓPQQ) was 5.10×10-11 mol/cm2 for the Au/Cu/PQQ-GDH-(His-tag) electrode prepared via the His-Cu affinity bond. In conjunction with the above results, it was therefore suggested that 97% of the total immobilized enzyme molecules were maintained in an electroactive state. Similarly, from IpPQQ (0.15×10-6 A (CV data not shown)), 4.19×10-11 mol/cm2 enzyme molecules were electroactive on the C/PQQ-GDH-(His-tag) electrode prepared via amide bonding.

Electrochemical properties of the highly oriented electrode containing the His-tag and the non-orientated electrode obtained using a cross-linking reaction were compared. The results are shown in Table 1. Although the orientation of each electrode was different, the ΓPQQ values were very similar between the two electrodes. There were few differences between the electrodes regarding electron transfer. According to the three-dimensional structure of PQQ-GDH [35], the attached PQQ was in the center of the enzyme; therefore, it is considered that the distance between PQQ and the electrode was almost identical (Figure 5a). Thus, in the case of DET of PQQ, the orientation of the enzyme did not affect the efficiency of electrochemical reaction.

Electrode Current Density (μA/cm2) IpPQQ(μA) Electroactive molecules (mol/ cm2)
Random Immobilization (Covalent Bond) 0.20 0.15 4.19 × 10-11
High-oriented Immobilization (His-tag affinity) 18.6 0.73 5.10 × 10-11

Table 1: Comparison of electrochemical properties in each electrode system.


Figure 5: Schematic illustration of electron-transfer process on the highly oriented or randomly oriented enzyme layer between PQQ-GDH and electrode (a) Direct electron transfer of PQQ (b) Electrochemical catalytic reaction. Orientation of the enzyme does not affect the electron transfer through the PQQ (a). However, the enzymatic reaction is inhibited if the catalytic site was located on the electrode surface (b).

The electroactive coverage on each electrode surface was calculated from the oxidization of PQQ and approximately the same for both electrodes. However, the current density (the current of PQQ was subtracted) of each electrode was quite different: 18.6 μA for the highly oriented electrode, and 0.20 μA for the non-oriented electrode. Therefore, it was shown that the highly orientated electrode was more efficient than the non-oriented electrode. In the highly orientated electrode, PQQ-GDH-(His-tag) is immobilized to Cu by a His-tag attached to the N-terminal of the enzyme. It is expected that the catalytic reaction is facilitated because accessibility of the substrate is not blocked, as the active site of the enzyme is located opposite to the electrode surface (Figure 5b). Because the enzyme molecules had different orientations on the non-oriented electrode surface, the efficiency was lower than that of the highly oriented electrode. These results demonstrated in this study will allow high-performance bio-fuel cells to be designed and constructed, and lead to practical usage.


In the present study, we developed an anode to realize a highly efficient reaction by focusing on affinity binding between Cu and Histag. In the study of electrodes for bio-fuel cell, the stability of the enzyme itself is a major challenge, but enzymes from hyperthermophiles can solve this problem because they have high thermostability and longterm stability. In the PQQ-GDH used in this study, PQQ is present inside the enzyme, and electron transfer is performed through the PQQ to provide a current density of 18.6 μA/cm2 without mediators. We determined the amount of enzyme that existing on the surface of the electrode, and showed that a highly efficient electrode reaction was achieved by orientation of the enzymes. The His-Cu affinity bond allowed proper orientation of the active site, thereby permitting an effective electrode reaction. By employing His-PQQGDH from the hyperthermophilic archaeon P. aerophilum with a high degree of orientation, it may become possible to develop a practical bioanode with long-term stability.


This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI (23510127) Grant-in-Aid for Scientific Research (C).


Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Relevant Topics

Article Usage

  • Total views: 12194
  • [From(publication date):
    April-2014 - Apr 24, 2018]
  • Breakdown by view type
  • HTML page views : 8357
  • PDF downloads : 3837

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version