Reach Us +44-1993-227344
Entomopathogenic Effect of Beauveria bassiana (Bals.) and Metarrhizium anisopliae (Metschn.) on Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)Larvae Under Laboratory and Glasshouse Conditions in Ethiopia
ISSN: 2157-7471
Journal of Plant Pathology & Microbiology

Like us on:

Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Entomopathogenic Effect of Beauveria bassiana (Bals.) and Metarrhizium anisopliae (Metschn.) on Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)Larvae Under Laboratory and Glasshouse Conditions in Ethiopia

Shiberu T* and Getu E

Department of Zoological Sciences, Addis Ababa University, College of Natural and Computational Sciences, Ethiopia

*Corresponding Author:
Shiberu T
Department of Zoological Sciences Addis
Ababa University, College of Natural and
Computational Sciences, Ethiopia
E-mail: [email protected]

Received date: March 06, 2017; Accepted date: May 24, 2017; Published date: May 27, 2017

Citation: Shiberu T, Getu E (2017) Entomopathogenic Effect of Beauveria bassiana (Bals.) and Metarrhizium anisopliae (Metschn.) on Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) Larvae Under Laboratory and Glasshouse Conditions in Ethiopia. J Plant Pathol Microbiol 8:411. doi:10.4172/2157-7471.1000411

Copyright: © 2017 Shiberu T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Journal of Plant Pathology & Microbiology


Tomato leaf miner, Tuta absolute (Meyrick) is one of the major pest that infest tomato plant in all agro-ecological regions of the world where it present. Currently, the management strategies highly rely on chemical insecticides, which do not provide effective control and at the same time have environmental concern in addition to the residue left on the fruits. Hence, looking for alternative control measure is vital. Studies were conducted to determine the pathogenicity and virulence of three different concentrations of Beauveria bassiana and Metarhizium anisopliae against larvae of T. absoluta using the concentrations of 2.5 × 107, 2.5 × 108, and 2.5 × 109 conidia ml-1 under laboratory and glasshouse conditions. The experiments were carried out in the laboratory and glasshouse. Mortalities caused by B. bassiana isolate at the different concentrations ranged from 79.17% to 95.83% under laboratory and 73.0% to 84.04% under glasshouse, the highest mortality percentage being found at 2.5 × 109 conidia ml-1. The isolate of M. anisopliae caused the highest mortality also at the highest concentration. The lowest lethal time for B. bassiana and M. anisopliae , were achieved by the concentration 2.5 × 109 (5.01 days) and 2.5 × 108 (5.21 days), respectively. The isolates of B. bassiana and M. anisopliae, at 2.5 × 109 conidia ml-1 are promising for use the integrated control of T. absoluta larvae.


Beauveria bassiana; Metarhizium anisopliae; Efficacy; Conidia concentrations; Larval mortality; Virulence; Chemical insecticides


Tomato leafminer, Tuta absoluta (Meyrick) is an oligophagous notorious pest of a number of economic crops including tomato. To overcome the problem of this pest, insecticides play a significant role globally. Tomato is a perishable commodity with a relatively short shelves life after harvest. This pest was initially reported in the central Rift Valley region of Ethiopia in 2012 [1]. Since the time of its initial detection, the pest has caused serious damages to tomato in invaded areas [2] and it is currently considered as a key threat to Ethiopian tomato production. If no control measures are taken, the pest can cause up to 80% to 100% yield losses by attacking leaves, flowers, stems and fruits [3]. Currently, chemical insecticides are heavily used by tomato growers against T. absoluta. However, the chemicals which are under use have negative impacts as the other chemical have. Hence, combination with other control methods like use of entomopathogen becomes imperative, as the continued use of chemical insecticides could harm non-target organisms [4] and the environment among others. The recommended waiting period which is required between application of conventional organophosphate pesticides group and consumption can hardly be afforded. Therefore, the current experiment was initiated to evaluate the efficacy of M. anisopliae and B. bassiana isolates against in the laboratory.

Materials and Methods

Description of the study area

The research was conducted under laboratory and glasshouse conditions at Ambo University glasshouse and plant Science laboratory. Ambo is far away from Addis Ababa 110 km and at geographical coordinate of 8°59`N latitude and 37.85°E longitude with an altitude of 2100 meter above sea level (m.a.s.l.) [5]. The mean daily temperatures were 22°C ± 2°C and 32°C ± 2°C for laboratory and glasshouse experiments, respectively.

Experimental design and materials used

The laboratory and glasshouse experiments were laid out in a Randomized Complete Block Design (RCBD) with three replications. Eight treatments were considered such treatments were Beauveria bassiana isolate at three different concentrations (2.5 × 107, 2.5 × 108 and 2.5 × 109 conidia ml-1), similar concentrations were performed in Metarhizium anisopliae isolate. Chlorantraniliprole (Coragen 200 SC)as a standard check and untreated control was also considered for comparision.

Tomato cultivar known as “Coshoro” was brought from Melkasa Agricultural Research Center. The seeds were sown on the field for natural infestation of T. absoluta. Harboring T. absoluta larvae were collected from the fields of tomato and brought to the laboratory and glasshouse. The tomato leaf miner present on these collected tomato leaves were wrapped with wet cotton kept in plastic box (20 × 15 cm2) in laboratory and glasshouse. After the emergency of adults rearing cages were prepared under glasshouse.

The insect was reared and maintained on tomato plants in the glasshouse until use. Leaves were examined under binocular microscope and T. absoluta larvae were counted. Spore suspensions were sprayed using a hand sprayer (1 liter of capacity). After treatment applications, the percent mortalities of the agents were observed at: 3, 5 and 7days in the laboratory and 3, 5, 7 and 10 days under glasshouse conditions.

Fungus culture and viability test

Isolates of Beauveria bassiana (PPRC-56) and Metarhizium anisopliae (PPRC-2) were obtained from Ambo Plant protectionresearch center. These entomopathogenic fungi were cultured on potato dextrose Agar (PDA) medium containing 20 g glucose, 20 g starch, 20 g agar, and 1000 ml of distilled water in test tubes. The test tubes containing PDA medium was autoclaved at 121°C for 15-20 min and incubated at 27°C ± 1°C, 80% ± 5% relative humidity and photophase of 12 h for 15 days. The relative humidity was measured using Huger Hygrometer. The conidia were harvested by scraping the surface of 14-15 days old culture gently with inoculation needle. The mixture was stirred with a magnetic shaker for 10 min. The hyphal debris was removed by filtering the mixture through fine mesh sieve. The conidial concentration of final suspension was determined by direct count using Haemocytometer. Serial dilutions were prepared in distilled water containing 0.1% Tween-80 and preservedat 5°C until used.

Conidial viability was assessed according to Goettel and Inglis [6]. Three different concentrations were evaluated. The droplet of a diluted suspension was placed on a thin film of potato dextrose agar medium incubated at 27°C ± 1°C and 80% ± 5% relative humidity in the dark for 24 h. The conidia were stained with lacto-phenol cotton blue and germination was observed under microscope.

Mortality of T. absoluta under laboratory

The concentration of the stock suspension was adjusted to 2.5 × 107. 2.5 × 108 and 2.5 × 109 conidia ml-1 using an improved neubaour heamocytometer. To evaluate the efficiency of each of the fungal isolates on T. absoluta, 20 larvae were placed on a filter paper in 9 cm diameter petri-dish and 100 μl of the suspension was then spread. On similar trend the suspension was spread in glasshouse using hand sprayed was performed and after 3rd days of observation all counted larvae were collected from plants to brought in the laboratory to determined how many T. absoluta larvae were dead without being infested with fungal isolates. A control treatment was sprayed with only sterile distilled water as negative control.

Statistical analysis

The mean number of live larvae per plant or per leaf was tested for percent mortality. The data was subjected to analysis of variance (ANOVA) and the means were compared by least significant different (LSD) test at 0.05 levels, using SAS program version 9.1 [7]. Efficacy analysis was done based on data transformation to Arcsineequation when necessary according to Gomez and Gomez [8].


Where: CM (%) - Corrected mortality

T- Mortality in treated insects

C- Mortality in untreated insects

Results and Discussions

Under laboratory condition

The laboratory result also showed that percent mortality of T. absoluta larvae due to entomopatogenic fungi significant (P<0.01)differences among the concentrations of B. bassiana and M. anisopliae (Table 1). All concentrations of B. bassiana caused mortality of T. absoluta above 75% after treatment application of 7 days, indicating that2.5 × 109 conidia ml-1 caused the highest mortality. For M. anisopliae, at the concentration of 2.5 × 109 conidia ml-1, mortalities obtained with all concentrations were higher than 50%; however, the concentrations did differ statistically from each other after treatment application, and the highest mortality of T. absoluta larvae were observed with concentration 2.5 × 109 (87.5%) under laboratory condition (Table 2).

Isolate code Host Place of collection Scientific Name % Germination Source
PPRC-56 P. interrupta Berbere B. bassiana 79 PPRC Ambo
PPRC-2 P. interrupta Ashan M. anisopliae 93 PPRC Ambo

Table 1: Pieces of information about indigenous entomopathogenic fungi in Ethiopia.

After 7th day of treatment application B. bassiana raveled that 79.17%, 83.33% and 95.83% mortality at 2.5 × 107, 2.5 × 108 and 2.5 × 109 concentrations, respectively. Similarly, M. anisopliae concentrations showed that 66.67%, 79.17% and 87.50% mortality at 2.5 × 107, 2.5 × 108 and 2.5 × 109 concentrations, respectively. There was a highly significant variation among the concentrations in causing mortality of T. absoluta larvae. The lowest mean percent mortality was caused by the B. bassiana at 3rd days of observation 37.50% which was not significantly different from M. anisopliae at 3rd days of 58.33 %. The highest mortality of T. absoluta was caused by B. bassiana 95.83% which did not significantlydiffer from the M. anisopliae which was 87.50% mortality. Based on the results of the virulence assays of B. bassiana and M. anisopliae had time taken by the three concentrations to caused percent mortality of T. absoluta. The effects of the concentrations varied significantly (P<0.01)with the lowest (3 days) recorded from concentration 2.5x107 in B. bassiana followed by M. anisopliae (5 days) which recorded 58.33%. Inthe 7th day of the three concentrations the highest was recorded due to B. bassiana which was significantly (P<0.01) different from M. anisopliae concentrations.

The comparison among the different concentrations and treatments against T. absoluta the results indicated good performance and gradually increased from 3 to 7 days treatment application. The percent mortality according to Abbott formula [9], both agents at 2.5 × 109 conidial/ml gave statistically no significant (P<0.01) differences from the standard check (Coragen 200 SC) while highly significant different from untreated check after 3 days of treatment application (Table 2).

Treatments Conc. Mean percent mortality after treatment application
3 days 5 days 7 days
2.5 × 107 37.50c 58.33c 79.17b
2.5 × 108 70.83bc 70.83bc 83.33ab
2.5 × 109 79.17ab 91.67ab 95.83a
2.5 × 107 58.33bc 58.33bc 66.67b
2.5 × 108 58.33bc 79.17bc 79.17ab
2.5 × 109 66.67bc 83.33abc 87.50ab
Chlorantraniliprole (Coragen 200 SC) 95.83a 95.83a 95.83a
Control (water) 0.0d 0.0d 0.0c

Table 2: Mean percent mortality of T. absoluta treated with fungal isolates at different concentration over time under laboratory condition.

Concentration-response test

Percent mortality of T. absoluta larvae at different concentrations of B. bassiana and M. anisopliae shown in Table 2. There were no significant differences in mortality rates within each concentration except for the concentration of 2.5 × 109 conidia/ml in which the B. bassiana showed significantly higher mortality than M. anisopliae. Theresults of all concentrations except the first concentration 2.5 × 10 in

B. bassiana revealed the lowest at 3rd days of application but also highlysignificantly (P<0.01) among the concentrations requiring higher concentration (2.5 109 × 109 conidia ml-1). B. bassiana, strain presented the highest pathogenicity on T. absoluta larvae with 95.83% an average mortality, LC50=2.5 × 109 conidia ml-1 and LT50=5.01 days (Table 3). M. anisopliae strain was the most virulent on T. absoluta larvae presenting 87.50% mortality,

Treatments      95% Confidence Limit 95% Confidence Limit
  (Conidia ml-1) LT50 (days) Slope ± SE LT90 (days) Slope ± SE
B. bassiana
2.5 × 107 5.45 3.17 ± 0.52 9.32 2.28 ± 0.48
2.5 × 108 5.21 3.80 ± 0.61 8.87 2.66 ± 0.37
2.5 × 109 5.01 4.29 ± 0.82 8.06 3.06 ± 0.68
M. anisopliae
2.5 × 107 5.14 3.64 ± 0.56 9.04 2.86 ± 0.46
2.5 × 108 5.02 3.63 ± 0.48 8.56 3.04 ± 0.58
2.5 × 109 4.82 3.31 ± 0.64 8.14 3.31 ± 0.72

Table 3: Median lethal time (LT50) and (LT90) of B. bassiana and M. anisopliae against T. absoluta.

LC50=2.5 × 109 conidia ml-1 and LT50=4.82 days. The LT90 values to B. bassiana strains on T. absoluta larvae ranged from 8.06 to 9.32 days,

and for M. anisopliae strains on T. absoluta larvae ranged from 8.14 to 9.04 days (Table 3). The M. anisopliae strain presenting the lowest LC90 on T. absoluta larvae was 2.5 × 109 conidia ml-1) and the highest LC90 was presented by B. bassiana 2.5 × 107 conidia ml-1). Finally, for

T. absoluta larvae the LC90 of both B. bassiana and M. anisopliae variedfrom 2.5 × 107 to 2.5 × 109 conidia ml-1 concentration (Table 4).

Treatments 95% Confidence Limit
  (Conidia ml-1) LC50 Slope ± SE LC90 Slope ± SE
B. bassiana
2.5 × 107 4.23 ± 0.52 4.29 ± 0.82 9.68 ± 0.82 3.36 ± 0.41
2.5 × 108 3.93 ± 0.61 3.80 ± 0.61 9.22 ± 0.61 2.64 ± 0.38
2.5 × 109 3.50 ± 0.82 3.17 ± 0.52 8.46 ± 0.52 2.45 ± 0.28
M. anisopliae
2.5 × 107 3.59 ± 0.56 3.31 ± 0.64 8.71 ± 0.64 2.47 ± 0.77
2.5 × 108 3.26 ± 0.48 3.63 ± 0.48 8.13 ± 0.48 2.63 ± 0.58
2.5 × 109 2.91 ± 0.64 3.64 ± 0.56 7.52 ± 0.56 3.54 ± 0.72

Table 4: Mean concentration (LC50) and (LC90) of B. bassiana and M. anisopliae (100µ /larva) of T. absoluta.

Under glasshouse conditions

The entomopathogenic fungal isolates were tested at three different concentrations for their percent mortality against T. absoluta in glasshouse to explore their potential to manage the pest population. Percent mortality of T. absoluta larvae were calculated for the different concentrations of the two isolates and showed increasing mortality with increasing spore concentration. Cumulative mortality of T. absoluta larvae over exposure period (3, 5, 7 and 10 days) was

significantly (P<0.01) different for fungi isolates (Table 5). On the 3rd days of exposure maximum mortality 91.84 recorded from standard check, while the untreated control had 2.78% mortality. These were significantly different from all concentrations of the fungal isolates. Among the concentrations of entomopathogenic fungi maximum percent mortality was recorded at 2.5 × 109 conidial ml-1 of B. bassiana (84.04%) followed by M. anisopliae (76.31%) on 10th day after treatment application. At the highest concentration of conidial ml-1, all B. bcassiana concentration gave the highest percent mortality (Table 5). The results indicated for pathogenicity of all the concentrations revealed that all of them are virulent, even three days after application causing significant mortality up to 64.05% when compared with untreated control.

Treatments Conc. Mean percent mortality after treatment application
3 days 5 days 7 days 10 days
2.5 × 107 43.85c 57.57bc 75.17ab 81.64ab
2.5 × 108 56.27bc 56.27bc 76.62ab 73.0abc
2.5 × 109 63.84b 67.05b 67.05bc 84.04ab
2.5 × 107  38.76c 42.93c 53.37c 53.37d
2.5 × 108 44.07c 51.98bc 61.49bc 64.65cd
2.5 × 109 64.05b 68.21bc 71.98abc 76.31bc
Chlorantraniliprole (Coragen 200 SC) 91.84a 91.84a 91.84a 91.84a
Control 2.78d 4.76d 4.76d 7.14e

Table 5: Mean percent mortality of Entomopathogenic fungi at different concentration on larvae T. absoluta under glasshouse condition.

A positive relationship was recorded between mortality percentages and concentrations among the B. bassiana and M. anisopliae concentrations. Concurrently, with the increase in conidiaconcentration, a reduction in LT50 was observed. Concentrations of 2.5 × 109 from B. bassiana, at the concentrations 2.5 × 108 and 2.5 × 109 conidia ml-1, presented the shortest lethal time (Table 5). These low values are probably associated to the presence of enzymes that aid in the process of penetration of the fungi [10].

The effect of entomopathogenic fungi were evaluated to determine the concentrations with high efficacy against larvae T. absoluta under laboratory and glasshouse conditions. Both fungal isolates were found to be pathogenic to T. absoluta. Though, there was a variation in their virulence against T. absoluta. The percent mortality for all the concentration gradually increased. The spore formation appeared on the larvae of T. absoluta took place after treatment exposure of the concentrations of the two isolates starting from the day three after treatment exposure, and thereby no hatched larvae were appeared in the concentrations of both isolates comparing the control treatment. The M. anisopliae in all concentrations were significantly less effective when compared with that of B. bassiana in terms of virulence. Virulence due to B. bassiana on 10th day was not significantly different from each other. This indicated that all B. bassiana concentrations were the best management option of T. absoluta. This finding confirms with earlier reports [11] who obtained high percent mortality during the evaluation time for B. bassiana and M. anisopliae.

The amount of conidia used should to attain a certain concentration and thus, achieving an efficacious penetration of the fungus on the insect cuticle and causing host death. Similar findings by Garcia et al. [12] were obtained, evaluating the insecticidal activity of B. bassiana strains and M. anisopliae on Spodoptera frugiperda and Epilachna varivestis larvae at six concentrations (104 to 109); B. bassiana strain was more virulent for E. varivestis larvae with a 93.3% mortality, LC50=1.20 × 106 conidia ml-1 and LT50=5.1 days. B. bassiana strain presented the highest mortality on S. frugiperda larvae (96.6%, LC50=5.92 × 103 conidia ml-1 and LT50=3.6 days). It was also reported by another authors differences among lethal times is a tool widely used in selecting strains, because it is interesting that the fungus quickly eliminate its host, as well [13]. These results are disagreed with Khalid et al. [14], evaluating the virulence of various strains of B. bassiana and M. anisopliae on G. mellonella larvae using 102, 103, 104, 105 and 106 conidia ml-1 concentration.

Thus, laboratory and glasshouse experiments suggested that B. bassiana and M. anisopliae have good effect on both egg and larvae of T. absoluta. Sabbour [15] also confirmed the effectiveness of both B. bassiana and M. anisopliae against larvae of T. absoluta under laboratoryand greenhouse. The same results were obtained by Sabbour and Singer [16]; Sabbour and Abdel-Raheem [17]. These results agree with our findings and Cabello et al. [18] where stated that; the higher mortality

of larvae under laboratory studies indicated B. bassiana could cause good larval mortality. At present, the knowhow of entomopathogenic fungi on T. absoluta was very limited because of very few studies that are available to indicate that the isolates causes the high mortality on other lepidopteran insects [19]. In this study it has been shown that all the fungal concentrations are effective against T. absoluta.

Our results confirmed that, the previous study of Shalaby et al. [11], they stated that when the second instar larvae fed on M. anisopliae the pathogen effect was evident by the 3rd day of evaluation after exposure in the concentration (107 and 108 conidia/ml). Dahliz et al. [20] have reported similar results with Metarhizium. Our result was confirmed the work of I?nanl and Oldargc [21], they reported the studies conducted in Turkey, researchers compared the efficacy of B. bassiana and M. anisopliae on T. absoluta eggs and larvae; these two agents provided highly effective to control of T. absoluta larvae. Our results also indicated the potential of B. bassiana and M. anisopliae to control the larvae of T. absoluta in an integrated pest management programs. Neves and Alves [22] also noted, as more conidia penetrating, more toxins or enzymes are released, increasing the insect mortality. Though, the fungus action speed depends, besides the concentration, of the host species involved [23]. According Kleespies and Zimmermann [24], variation in virulence of entomopathogenic strains is a result of differences in the enzymes and toxins production in conidia germination speed, mechanical activity in the cuticle penetration, colonization capacity and cuticle chemical composition.

Conclusion and Recommendation

The most effective percent mortality of fungal isolates was found in B. bassiana followed by M. anisopliae at all concentrations. Both agents could be very well utilized as alternative to bio pesticides for the management of T. absoluta. It might be concluded that B. bassiana and M. anisopliae fungi present different capacity cause mortality of the insects, with the 2.5 × 109 conidian ml-1 B. bassiana strains as the most pathogenic for T. absoluta, as well as 2.5 × 109 conidian ml-1 M. anisopliae strains was also good virulence for T. absoluta and also presenting the lowest LC50 and LT50 values. Hence, insecticidal substances that have potential for use as alternative control measure. Therefore, further study on field conditions should be undertaken to evaluate effectiveness of experimental mycopesticide formulations in the management of T. absoluta under Tropical conditions in various economically important insect pests.


The authors would like to acknowledge Ambo University and Addis Ababa

University for financial support. We greatly appreciate Ambo University, College of

Agriculture and Veterinary Sciences, Department of Plant sciences, for allowing us to have access to their glasshouse to do the experiment. We also appreciate Mr.

Fikadu Balcha for his technical assistance in data collection.


Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Relevant Topics

Recommended Conferences

Article Usage

  • Total views: 1334
  • [From(publication date):
    May-2017 - Nov 17, 2018]
  • Breakdown by view type
  • HTML page views : 1200
  • PDF downloads : 134

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri and Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version