Eosinophilic Esophagitis and Ige-Mediated Allergy in Children: Specific Ige by Component-Based-Allergen Microarray

EoE is a chronic immuno-allergic-inflammatory disease related to multiple factors. According to Furuta et al. [1] diagnosis of EoE included clinical suspicion, ≥ 15 eosinophils/HPF and exclusion of other diseases such as GERD. In 2011, Liacouras et al. [2] introduced ‘‘proton pump inhibitors-PPI-responsive esophageal eosinophilia” to identify patients responsive to PPI therapy [2]. Endoscopy with biopsies represents the first step in defining EoE.


Introduction Methods
Thirty consecutive patients (Male: 23) affected by EoE according to the criteria of Furuta et al. [1] and Liacouras et al. [2] were enrolled. All patients with clinical signs of hypersensitivity to specific substances were considered allergic, regardless of the presence of positive testing.
In all patients SPT, ImmunoCAP and ISAC microarray tests were performed. The study was approved by the Hospital Ethical Committee and an informed consent was obtained from parents before enrolment. Laboratory personnel were not informed about the results of the SPT, performed under the control of a pediatric allergist.

Skin prick test
SPT were performed with commercial natural extracts to suspected triggering food and airborne allergens for each patient.
Hence, different patients were tested with different panels of suspected allergens according to the clinical history the diagnostic routine and guidelines used at the hospital. The allergens used were: foods (milk, α-lactalbumin, β-lactoglobulin, casein, egg white, egg yolk, soybean, rice, wheat, corn, beef, chicken codfish, carp, tomato, potato, peanut and hazelnut), inhalant (dust mite, timothy grass, wall pellitory, olive, cat and dog dandruff) and with sodium chloride saline (0.9%) and histamine hydrochloride (Lofarma, Milan, Italy). The response was read 15 minutes after puncture and results expressed as the mean wheal diameter (mm). The appearance of erythema with a diameter >3 mm was defined as a positive reaction.

Allergen microarray assay
All patients were tested with the same panel of 103 allergenic molecules. The commercially available allergen chips were purchased from Phadia AB (Uppsala, Sweden) and the assay performed according to the instruction provided by the manufacturer [13]. A customized version of the microarray (ISAC TM version CRD103) containing 103 purified or recombinant allergenic molecules was used. Chips were washed for one hour in the washing buffer, rinsed and dried. 20 µL of undiluted serum was applied onto each reaction well. Chips were incubated for 2 hours at room temperature in a humid chamber, rinsed and washed twice in washing buffer and once in deionized water. Chips were incubated for 1 hour at room temperature with 20 µL of an Alexa 546-labelled anti-human IgE antibody, washed, dried and stored in the dark until scanning. Scan Array Gx Scanner (Perkin-Helmer, Boston, MA) with two laser power settings was used in order to achieve a maximum dynamic range across different levels of IgE concentrations.
Images were analysed using the MIA software (Version 3.1; Phadia AB) and sIgE were quantified as ISU (ISAC Standardized Units).
Food impaction was the onset symptom in 9 patients; the other patients presented a specific symptom of EoE at diagnosis (abdominal pain, dysphagia, heartburn, vomiting, failure to thrive). In thirteen out of 30 patients (43%) peripheral eosinophilia was present.

Comparison of microarray results with extract-based ImmunoCAP and SPT results
For 22 out of 30 patients microarray results were in agreement with the results obtained with traditional diagnostics (Table 3).  Any clinically "false" positive results on ISAC were not observed. Neither did ISAC miss any allergy-provoking allergens according to doctor's diagnosis and open challenge test.
In 5 out of 30 patients milk was detected low positive on ImmunoCAP (1.5, 1.7, 1.9, 2.0 and 2.8 kU/l respectively), and tested negative on ISAC. These patients did not show any symptoms upon open challenge for milk.

New information provided by microarray test
For 8 out of 30 patients ISAC gave new, relevant diagnostic information which were not possible to obtain from the traditional tests (SPT or ImmunoCAP). The new information was either detection of cross-reactive molecules or identification of unsuspected allergens (Table 4).   Thirteen out of the 16 patients tested positive with ISAC were multi-sensitized (i.e. sensitized to 3 or more different types of allergens, e.g. grass, mite and mold or 3 different pollen species like birch, olive and grass).

Discussion
In our study, we limited evaluations to immediate allergic reactions. The high reported prevalence of respiratory allergy, commonly linked to immediate processes, is confirmed in our experience. A previous study on 76 adults using ISAC (112 allergens) demonstrated that 74% of patients had aeroallergens sensitization and that birch pollen sensitization (r Bet v1) had cross reaction with some food allergens [15]. This study showed that patients had poly-sensitization to food and aeroallergens and allergens identified will be useful to direct dietary therapy.
A good correlation between the measurement of sIgE with allergen microarray and the clinical signs was found for inhalant molecules (dust mite and birch), panallergens (latex, kiwi) and food (fish). The number of positive allergens identified with ISAC was higher than ImmunoCAP. The high sensitivity of sIgE detection with ISAC improved identification of sensitized patients amenable to appropriate prophylaxis and possible specific therapy. Thirteen of 16 patients tested positive with ISAC were multi-sensitized (i.e. sensitized to 3 or more different types of allergens, e.g. grass, mite and mold or 3 different pollen species like birch, olive and grass).
For 8/30 patients ISAC gave new, relevant diagnostic information, not obtained before with traditional tests (SPT or ImmunoCAP): panallergens, molecules cross-reacting with the more common allergens. The panallergens encompasses families of related proteins, involved in general vital processes and thus, widely distributed throughout nature. They are responsible for many IgE cross-reactions even between unrelated pollen and plant food allergen source. Although usually considered as minor allergens, sensitization to panallergens might be problematic as it bears the risk of developing multiple sensitizations.
In this study only 8 patients had food hypersensitivity; we couldn't identify non-IgE-mediated food reaction. Therefore it is essential in a future study use a test for the determination of non-IgE mediated reactions in the EoE patients.
In our population, microarrays are in agreement with ImmunoCAP and SPT. Also, ISAC didn't miss any allergens related to patients' symptoms. In addition, the microarray allows a targeted therapy: seasonal anti-inflammatory treatment, specific immunotherapy and dietary restriction based on the identification of cross-reacting molecules. The quality of the microarray is good enough compared to traditional diagnostic tests, open challenge test and clinical diagnosis.