Reach Us +44-1522-440391
Epigenetic Regulation: Neurite Outgrowth by Hormonal or Chemical Mechanisms in PC12 Cells | OMICS International
ISSN: 2155-9538
Journal of Bioengineering & Biomedical Science

Like us on:

Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on Medical, Pharma, Engineering, Science, Technology and Business
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Epigenetic Regulation: Neurite Outgrowth by Hormonal or Chemical Mechanisms in PC12 Cells

Koji Shimoke*

High Technology Research Core (HRC), Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, Yamatecho, Suita, Osaka, Japan

*Corresponding Author:
Koji Shimoke
High Technology Research Core (HRC)
Department of Life Science and Biotechnology
Faculty of Chemistry, Materials and Bioengineering
Kansai University, Yamate-cho, Suita
Osaka 564-8680, Japan
Tel: +81-6-6368-0853, +81-6-6330-3770
E-mail: [email protected]

Received Date: December 27, 2016; Accepted Date: December 30, 2016; Published Date: January 02, 2017

Citation: Shimoke K (2016) Epigenetic Regulation: Neurite Outgrowth by Hormonal or Chemical Mechanisms in PC12 Cells. J Bioengineer & Biomedical Sci 6: e123. doi: 10.4172/2155-9538.1000e123

Copyright: © 2016 Shimoke K. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Journal of Bioengineering & Biomedical Science


In the embryonic stage, cells divide and migrate to achieve differentiation. Neurite outgrowth is involved in forming precise neuronal networks in neuronal differentiation, and can be mimicked in vitro using specific reagents. For instance, nerve growth factor (NGF), which was identified by Levi-Montalcini and Booker, strongly promotes neurite outgrowth in cells from the peripheral nervous system (PNS) [1]. A similar effect is seen in pheochromocytoma 12 (PC12) cells, which were established by Greene and Tischler [2,3]. In the four decades since establishment of these cells, many hormones (including peptides) and chemicals have been shown to promote neurite outgrowth in PC12 cells [4,5]. Surprisingly, epidermal growth factor (EGF), which is associated with cell division, promotes neurite outgrowth through a specific intracellular mechanism [4]. cAMP, an activator of intracellular protein kinase A (PKA), has also been reported to promote neurite outgrowth [5]. Evidence for a role of the nur77 gene in neurite outgrowth has been found, and the mechanism involves epigenetically regulated gene expression because it is influenced by trichostatin A, a histone deacetylase (HDAC) inhibitor that changes heterochromatin to euchromatin and has a similar effect to that of histone acetyl transferase (HAT)-induced gene expression in a specific region of the genome [5,6]. Generally, methylation of lysine 9 or 27 in histone H3 suppresses gene expression in association with chromodomain-containing negative transcriptional regulators, and methylation on lysine 4 induces gene expression. These epigenetic phenomena also depend on lysine acetylation in histone H3. Our study of neurite outgrowth in PC12 cells showed the importance of acetylation of lysine 14 in histone H3 in this process [6]. However, the role of demethylation of CpG sequences in the upstream sequence of the nur77 gene was unclear. Moreover, modification of histone H4 also requires analysis to develop a complete understanding of the mechanism of neurite outgrowth. These findings may just represent an initial understanding of epigenetic mechanisms in this field.

Research Support

KAKENHI(16K00626), SENRYAKU(2013-2017).


Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Relevant Topics

Recommended Conferences

Article Usage

  • Total views: 1884
  • [From(publication date):
    December-2016 - Jul 20, 2019]
  • Breakdown by view type
  • HTML page views : 1756
  • PDF downloads : 128