alexa Epitope Driven Broad Spectrum Peptide Vaccines against MDR Pathogens | Open Access Journals
ISSN: 2157-7560
Journal of Vaccines & Vaccination
Like us on:
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Epitope Driven Broad Spectrum Peptide Vaccines against MDR Pathogens

Prince Sharma1*, Neena Capalash2 and Ravinder Singh1

1Department of Microbiology, Panjab University, Chandigarh, India

2Department of Biotechnology, Panjab University, Chandigarh, India

*Corresponding Author:
Prince Sharma
Department of Microbiology
Panjab University, Chandigarh, 160014, India
Tel: 91-9815957903
E-mail: [email protected]

Received Date: July 01, 2016; Accepted Date: July 02, 2016; Published Date: July 05, 2016

Citation: Sharma P, Capalash N, Singh R (2016) Epitope Driven Broad Spectrum Peptide Vaccines against MDR Pathogens. J Vaccines Vaccin 7:e127. doi: 10.4172/2157-7560.1000e127

Copyright: © 2016 Sharma P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Journal of Vaccines & Vaccination


Acinetobacter baumannii, Pseudomonas aeruginosa and Klebsiella pneumonia have evolved as a group of co-existing multidrug resistant nosocomial pathogens spreading worldwide. Overuse of antibiotics has led to the emergence of pan drug resistant bacterial strains and has made all antimicrobial agents superfluous including carbapenems and even the last resort antibiotic-colistin, resulting in high mortality rates among the hospitalized patients. Vaccination is highly effective and successfully used strategy that reduces the incidence of infectious diseases, yet efforts towards development of vaccines for nosocomial infections are scant. In this direction, a broad spectrum vaccine is the futuristic need providing protection not only against A. baumannii but against the whole group of co-existing nosocomial pathogens.

The availability of complete bacterial genome and proteome sequences has revolutionized the approach of in silico vaccine prediction and development. Although there are a number of uncharacterized and putative proteins in the proteomes of the pathogens but the availability of genomic sequences and functional characterization of several genes involved in virulence has significantly increased our understanding of the molecular basis of pathogenesis and provides a wealth of information that can be used to design new approaches for effective vaccine development. Reverse vaccinology is a time effective technique that can predict potential epitopes as vaccine candidates by in silico analysis of bacterial proteomes and facilitates there in vivo validation [1], that ameliorates the treatment options against pathogens. In certain cases where natural immunogens do not induce optimal response and recombinant subunit vaccines become difficult to produce, epitope based approaches may solve the problem. Epitopes can be easily synthesized, formulated and validated in mouse models. Researchers are hopeful that in the near future synthetic peptide vaccine will be more effective, safe and long lasting compared to conventional ones. The ability of peptide vaccines to elicit both antibodies and cytotoxic T lymphocytes (CTLs) distinguishes them from conventional vaccines comprised of live attenuated or killed whole organism or purified antigen and makes them a promising vaccine approach to test on many pathogens.

The success of peptide vaccines may be attributed to the fact that peptides are selected by extensive in silico analysis using both sequence based and structure based screening methods. Because of their defined chemical nature and small size, synthetic peptides offer distinct advantages in terms of chemical characterization and manufacturing. The conventional method for T cell epitopes identification involves the experimental screening of overlapping peptides in the protein of interest which is a costly and time consuming task. Nevertheless, the development of various epitope predicting programs has facilitated the prediction of most promising epitope candidates and reduces the number of peptides selected for experimental validation. Outer membrane proteins conserved in different bacterial genera can be picked for prediction of identical epitopes prevalent in majority of bacterial proteomes. An important feature of epitope driven vaccine is epitope promiscuity that makes it a more relevant immunogen in genetically variable species such as human populations [2]. Therefore, an ideal peptide vaccine (peptope) could be designed by screening the complete pool of epitopes for most conserved, surface exposed ones that bind to majority of HLA alleles. These synthetic peptide vaccines can be conjugated with metalloprotein such as Keyhole limpet hemocyanin (KLH) [3] or can be used as such [4]. Examples of synthetic techniques used for their production involve the lipid core peptide vaccine delivery system, thioether ligation, multi-epitope vaccine prepared by polymerization approach, asymmetrical dendrimers produced with the help of copper-catalyzed azide-alkyne cycloaddition, multi-epitope construct produced by random polymerization of several acrylate modified B cell epitopes, recombinant polyepitope conjugated to adjuvant moiety with the help of intein and native chemical ligation and glycopeptides-based antigen synthesized in a mixed chemical/enzymatic approach [5]. The designed peptide vaccine when injected alerts the host’s immune system to generate immunization memory cells. Although peptope is an acceptable approach for a candidate vaccine [4,6] however, its efficacy must be validated by clinical trials.

The reduced side effects, cost effectiveness and improved stability of epitope driven peptide based vaccines can provide a major breakthrough in the vaccine development against pathogens. Further, stitching prominent epitopes conserved in the proteomes of a group of nosocomial pathogens can lead to a promising vaccine candidate that can provide broad spectrum protection. We expect a quantum leap in treatment options by developing epitope driven vaccines in future.


Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Recommended Conferences

Article Usage

  • Total views: 8387
  • [From(publication date):
    October-2016 - Sep 23, 2017]
  • Breakdown by view type
  • HTML page views : 8311
  • PDF downloads :76

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version