Evaluation of Biofield Treatment on Physical and Thermal Characteristics of Selenium Powder

The importance of selenium (Se) in human is well established, and its deficiency has caused serious diseases such as cancer and heart disease [1]. Around 25 selenoproteins contain selenium as important element, which are commonly known as selenocysteine [2]. In addition, research on selenium has gained significant attention due to its important role in antioxidant selenoproteins for protection against oxidative stress initiated by excess reactive nitrogen species (RNS) and reactive oxygen species (ROS) [1]. In selenocysteins, selenium metal functions as redox centre in various processes, which includes the reduction of nucleotides in DNA synthesis through selenoenzyme and thioredoxin reductase; and production of active thyroid hormone pre-cursor from selenium-dependent iodothyronine deiodinases [3]. Furthermore, the bioavailability of selenium plays a crucial role in its anticancer and antioxidant activities. It is reported that the particles with crystallite size of nanometre range exhibit better bioavailability and low toxicity as compared to particles with large crystallite size [4-6]. After considering the vast importance of selenium as nutrient in human body, authors wish to investigate an economically safe approach that could be beneficial to modify the physical and thermal properties of selenium powder.


Introduction
The importance of selenium (Se) in human is well established, and its deficiency has caused serious diseases such as cancer and heart disease [1]. Around 25 selenoproteins contain selenium as important element, which are commonly known as selenocysteine [2]. In addition, research on selenium has gained significant attention due to its important role in antioxidant selenoproteins for protection against oxidative stress initiated by excess reactive nitrogen species (RNS) and reactive oxygen species (ROS) [1]. In selenocysteins, selenium metal functions as redox centre in various processes, which includes the reduction of nucleotides in DNA synthesis through selenoenzyme and thioredoxin reductase; and production of active thyroid hormone pre-cursor from selenium-dependent iodothyronine deiodinases [3]. Furthermore, the bioavailability of selenium plays a crucial role in its anticancer and antioxidant activities. It is reported that the particles with crystallite size of nanometre range exhibit better bioavailability and low toxicity as compared to particles with large crystallite size [4][5][6]. After considering the vast importance of selenium as nutrient in human body, authors wish to investigate an economically safe approach that could be beneficial to modify the physical and thermal properties of selenium powder.
It is well established that all atoms are in motion, which contain significant amount of energy. This energy exists in the form of translational, rotation or vibrational, which is evident from vibrational spectroscopy. Similarly, cells of human body consist of various vibratory particles including electron, proton, ions etc. Further, due to vibrations of these particles, electromagnetic radiation emitted and that form an electromagnetic field around the body, known as biofield [7]. Thus, a human has the ability to harness the energy from the environment/universe and can transmit it to any object (living or nonliving) around the Globe. The object(s) always receive the energy and responded it into useful way that is called biofield energy. This energy healing process is termed as biofield treatment. The National Center for Complementary and Alternative Medicine (NCCAM) considered this biofield treatment (therapy) in subcategory of energy therapies [8]. Mr. Trivedi's unique biofield energy is also known as The Trivedi Effect ® . Mr. Trivedi's biofield energy treatment is known to alter the physical, structural and atomic level in various metals [9][10][11] and ceramics [12,13] in material science. In addition to that, impact of biofield treatment has been significantly studied in the field of microbiology research [14], biotechnology research [15], and agriculture research [16]. Recently, it was reported that biofield treatment had enhanced the crystallite size by two fold in zinc powder [17]. In addition, biofield treatment has significantly altered the crystallite size, unit cell parameter, surface area of magnesium, which is an important micronutrient for human body [18]. Based on the outstanding result achieved with biofield treatment on metals and ceramics, an attempt was made to evaluate the effect of biofield treatment on physical and thermal properties of Abstract Selenium (Se) is an essential trace element, and its deficiency in the humans leads to increase the risk of various diseases, such as cancer and heart diseases. The objective of this study was to investigate the influence of biofield energy treatment on the physical and thermal properties of the selenium powder. The selenium powder was divided into two parts denoted as control and treated. Control part was remained as untreated and treated part received Mr. Trivedi's biofield energy treatment. Both control and treated selenium samples were characterized using x-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis -differential thermal analysis (TGA-DTA), and Fourier transform infrared spectroscopy (FT-IR). The XRD data showed that biofield energy treatment has slightly altered the lattice parameter (0.07%), unit cell volume (0.15%), density (-0.14%), atomic weight (0.15%), and nuclear charge per unit volume (-0.21%) in the treated selenium powder as compared to the control. The crystallite size of the treated selenium powder was reduced considerably from 106.98 nm (control) to 47.55 nm. The thermal analysis study showed that the latent heat of fusion was 64.61 J/g in the control, which changed to 68.98, 52.70, 49.71 and 72.47 J/g in the treated T1, T2, T3, and T4 samples respectively. However, the melting temperature did not show any considerable change in the treated selenium samples as compared to the control. The FT-IR spectra showed the absorption peak at 526 and 461 cm -1 , which corresponding to metal oxide bonding vibration in the control and treated selenium powder respectively. Hence, overall data suggest that, the biofield energy treatment considerably altered the physical and thermal properties of selenium powder. Therefore, biofield energy treatment could make selenium even more useful nutrient in human body.
control samples, respectively. Similarly, the percent change in all other parameters such as unit cell volume, density, atomic weight, nuclear charge per unit volume and crystallite size were calculated.

Thermal analysis
Thermal analysis of control and treated selenium powder were performed using TGA-DTA and DSC. For DSC analysis, a Pyris-6 Perkin Elmer DSC at a heating rate of 10ºC/min under air atmosphere was used. Predetermined amount of sample was kept in an aluminum pan and closed with a lid. A blank aluminum pan was used as a reference. From DSC melting point and latent heat of fusion were recorded. For TGA-DTA analysis, Mettler Toledo simultaneous TGA and Differential thermal analyser (DTA) was used. The samples were heated from room temperature to 400ºC with a heating rate of 5ºC/ min under air atmosphere. From TGA curve, percent weight loss was noted. The melting point and latent heat of fusion of control and treated selenium were recorded from DTA curve. The percent change in melting point was computed using following equations:

Fourier transform infrared spectroscopy (FT-IR)
FT-IR spectroscopic analysis was carried out to evaluate the impact of biofield treatment at atomic and molecular level like bond strength, stability, and rigidity of structure etc. FT-IR analysis of control and treated selenium samples were performed on Shimadzu, Fourier transform infrared (FT-IR) spectrometer with frequency range of 300-4000 cm -1 .

Materials and Methods
The selenium powder was purchased from Alpha Aesar, Hyderabad, India. The sample was equally divided into two parts, considered as control and treatment. Control parts remained as untreated, whereas treatment group was in sealed pack and handed over to Mr. Trivedi for biofield treatment under laboratory condition. Mr. Trivedi provided the biofield treatment through his energy transmission process to the treated group without touching. The control and treated samples were characterized using XRD, DSC, TGA-DTA, and FT-IR spectroscopy.

X-ray diffraction study
XRD analysis of control and treated selenium powder was carried out on Phillips, Holland PW 1710 X-ray diffractometer system, which had a copper anode with nickel filter. The radiation of wavelength used by the XRD system was 1.54056 Å. The data obtained from the XRD system were in the form of a chart of 2θ vs. intensity and a detailed table containing peak intensity counts, d value (Å), peak width (θ°), relative intensity (%) etc. Additionally, PowderX software was used to calculate the lattice parameter and unit cell volume of selenium powder samples. Total nuclear charge was calculated as the number of protons multiplied by charge on a proton (1.6 × 10 -19 C). Nuclear charge per unit volume was computed as follow: Total nuclear charge in an atom Nuclear charge per unit volume Volume of an atom = Further, the crystallite size (G) was calculated by using Scherrer formula: Here, λ is the wavelength of radiation used, b is full width half maximum (FWHM) and k is the equipment constant (0.94). Furthermore, the percent change in the lattice parameter was calculated using following equation:   [18]. In addition, the intense peaks in control and treated sample suggested the crystalline nature of selenium powder. Crystal structure parameters were computed using PowderX software such as lattice parameter, unit cell volume, density, atomic weight, nuclear charge per unit volume. The results are presented in Table 1. Result showed that lattice parameter and volume of unit cell were slightly increased by 0.07 and 0.15% in treated selenium as compared to control. The increase of unit cell volume may induce internal strain in treated selenium powder. In addition, the atomic weight of treated selenium was slightly increased by 0.15% as compared to control. However, density and nuclear charge per unit volume was decreased by 0.14 and 0.21%, respectively as compared to control. The crystallite size computed using Scherrer formula was found as 106.98 nm in control and it was reduced to 47.55 nm in treated selenium powder. It suggests that crystallite size of treated selenium sample was significantly reduced by 55.55% as compared to control. Fuse et al. reported that the energy produced through mechanical milling had reduced the crystallite size of selenium powder and induced lattice strain in crystal structure [19]. Thus, it is assumed that biofield treatment might induce energy milling in selenium powder and that might be responsible for decrease in crystallite size of treated selenium powder. Recently, our group reported that biofield treatment had reduced the crystallite size in aluminium [20] and magnesium powder [17].
Moreover, selenium is a primary nutrient for human body, thus its bioavailability is an important parameter. It is demonstrated that the rate of dissolution can be altered by choosing a suitable polymorph of a compound i.e. the solids with low crystallinity or high amorphous phase exhibits higher solubility [21]. Torrado et al. reported that solids with small crystallite size exhibits faster dissolution rate as compared to solids with higher crystallite size [22]. Thus, it is hypothesized that biofield treated selenium powder may exhibit the higher dissolution rate, since it has smaller crystallite size as compared to control. The increase in dissolution rate may lead to increase bioavailability of selenium in human gastric fluids.

Thermal analysis
Thermal analysis of selenium powder was carried out using TGA-DTA and DSC. Thermal analysis results of control and treated selenium powder samples using DSC are illustrated in Table 2. DSC showed the melting point of 221.66°C, 222.90°C, 222.16°C, and 222.12°C in control, T1, T2 and T3 respectively. The latent heat of fusion was found as 73.17, 73.32, 73.11, 73.85 J/g in control, T1, T2 and T3 respectively. DSC data showed no significant change in melting point and latent heat of fusion in selenium. Furthermore, TGA-DTA analysis results of control and treated selenium samples are presented in Table 3. DTA results showed the melting temperature of 220.92°C, 220.82°C, 220.50°C, 220.99°C and 221.99°C in control, T1, T2, T3, and T4 respectively. It suggests that melting temperature was slightly changed after biofield treatment. Further, TGA data showed the percent weight loss of 7.01, 9.05, 3.80, 6.81, and 0.34% in control, T1, T2, T3, and T4 respectively. Furthermore, DTA integral area was found as 102.66 s°C in control, which changed to 132.13 s°C, 40.38 s°C, 58.08 s°C and 216.56 s°C in T1, T2, T3, and T4, respectively. These integral area data were further used to calculate the latent heat of fusion of respective control and treated selenium samples. Data showed the latent heat of fusion of 64.61 J/g in control selenium powder, which changed to 68.98, 52.70, 49.71 and 72.47 J/g in T1, T2, T3, and T4 samples, respectively. It indicated that latent heat of fusion was increased by 6.76 and 12.61% in treated selenium powder T1 and T4, whereas it was reduced by 18.43 and 23.07% in treated T2 and T3 samples as compared to control.
Fundamentally, the energy absorbed by any compound during phase change (i.e. solid→liquid) is known as latent heat of fusion. During this process, latent heat of fusion is utilized to overcome the interatomic interaction in solids to change the phase. In phase change process, latent heat of fusion leads to increase the distance between atoms and stored in atoms as potential energy. Further, the solids with higher interatomic interaction exhibit the higher latent heat of fusion and vice versa. Thus, based on this, it is assumed that biofield treated samples (T1 and T4) probably have higher interatomic interaction as compared to control, which may lead to increase the latent heat of fusion. Contrarily, treated selenium samples T2 and T3 possibly have lower interatomic interaction. It is assumed that the alteration of interatomic interaction in treated selenium powder could be due to change in lattice parameter of unit cell through biofield treatment. It is reported that selenium reacts with various enzymes due to its redox nature i.e. it can oxidise as well as reduce the enzymes [23]. Further, in biological activities, the interatomic interaction of selenium plays a major role. Thus, the reduction of interatomic interaction in selenium powder after biofield treatment may affect the interaction of selenium with enzymes human body.

Fourier transform infrared spectroscopy (FT-IR)
The FT-IR spectra of control and treated selenium powders are given in Figure 2a and 2b, respectively. In FT-IR spectra, the absorption peaks were observed at 526 cm -1 in control, whereas 461 and 709 cm -1 in treated selenium sample, which can be attributed to metal -oxygen stretching vibration. This peak may be attributed to oxidation of selenium powder [24]. The alteration in absorption peaks could be due to biofield treatment. Furthermore, the absorption peak were observed at 3774 cm -1 in control and 3751 cm -1 in treated selenium powder, which could be due O-H stretching vibrations, which emerged    to moisture absorption by powders. Hence, FT-IR data revealed that biofield treatment has slightly altered the bonding properties of selenium powder.

Conclusion
The XRD results showed that the crystallite size was reduced by 55.55% in treated selenium sample as compared to the control, which could be due to the internal strain induced through biofield energy treatment. The reduction in the crystallite size may increase the bioavailability of treated selenium powder in gastric fluid. Thermal analysis indicated that latent heat of fusion was increased by 6.76 and 12.16% in T1 and T4, whereas it was reduced by 18.43 and 23.07% in T2 and T3, respectively as compared to the control. Based on the alteration in the latent heat of fusion in treated samples, it is assumed that biofield energy treatment might alter the inter-atomic interaction of the treated selenium powder. The change in interatomic interaction in treated selenium may alter its catalytic activities in human body. Hence, overall study concludes that biofield energy treatment has significantly altered the physical and thermal properties of the selenium powder. Therefore, the biofield energy treated selenium could be more useful as nutrient in human body.