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ISSN: 2329-6895
Journal of Neurological Disorders
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Expanded Central Nervous System Gene Transfer in Rats by Intravenous Delivery of Recombinant Adeno-Associated Virus

Kasey L. Jackson1*, Blas S. Catalani2, Robert D. Dayton1 and Ronald L. Klein1
1Department of Pharmacology, Toxicology, and Neuroscience, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
2Department of Anesthesiology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
Corresponding Author : Kasey L. Jackson
Department of Pharmacology
Toxicology, and Neuroscience
Louisiana State University Health Sciences Center
Shreveport, LA 71130, USA
Tel: 2255783202
E-mail: [email protected]
Received November 10, 2014, Accepted November 12, 2014, Published November 14, 2014
Citation: Jackson KL, Catalani BS, Dayton RD, Klein RL (2015) Expanded Central Nervous System Gene Transfer in Rats by Intravenous
Delivery of Recombinant Adeno-Associated Virus. J Neurol Disord 3:i109. doi: 10.4172/2329-6895.1000i109
Copyright: © 2015 Jackson KL, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Viral vector gene transfer is an important tool for studying protein function in vivo. Recombinant adeno-associated virus (AAV) is advantageous for gene transfer to the central nervous system. Over the past few years it has been shown that an intravenous vector delivery can transduce neurons throughout the central nervous system [1-3]. The images are of rats administered AAV serotype 9 vector encoding green fluorescent protein (GFP). The rats were injected intravenously on post-natal day one and imaged for GFP expression 4-12 weeks later. There is robust expression in the spinal cord and dorsal root ganglia and the cerebellum (Figures 1-3). We have been exploiting the efficient spinal cord transduction to study a spinal cord disease, amyotrophic lateral sclerosis [2-4].

Description
Viral vector gene transfer is an important tool for studying protein function in vivo. Recombinant adeno-associated virus (AAV) is advantageous for gene transfer to the central nervous system. Over the past few years it has been shown that an intravenous vector delivery can transduce neurons throughout the central nervous system [1-3]. The images are of rats administered AAV serotype 9 vector encoding green fluorescent protein (GFP). The rats were injected intravenously on post-natal day one and imaged for GFP expression 4-12 weeks later. There is robust expression in the spinal cord and dorsal root ganglia and the cerebellum (Figures 1-3). We have been exploiting the efficient spinal cord transduction to study a spinal cord disease, amyotrophic lateral sclerosis [2-4].
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