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ISSN: 2155-9937
Journal of Molecular Imaging & Dynamics
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Gastrointestinal Stromal Tumors: A Review

Farhat Abbas1, Waseem Raja Dar2*, Muzamil Latief2, Summyia Farooq3, Manzoor Ahmad Parry2, Peerzada Ziaulhaq4, Najeeb ullah Sofi2 and Imtiyaz Dar2

1Department of Pathlogy, Sher-i-kashmir Institute of Medical Sciences, Srinagar, India

2Department of Medicine, Sher-i-kashmir Institute of Medical Sciences, Srinagar, India

3Department of Pathology, Government Medical College, Srinagar, India

4Department of Radiology, Sher-i-kashmir Institute of Medical Sciences, Srinagar, India

*Corresponding Author:
Waseem Raja Dar
Department of Medicine
Sheri-kashmir Institute of Medical Sciences, Srinagar, India
Tel: +91-8261815278
E-mail: [email protected]

Received date: February 09, 2016; Accepted date: February 25, 2016; Published date: February 27, 2016

Citation: Abbas F, Dar WR, Latief M, Farooq S, Parry MA, et al. (2016) Gastrointestinal Stromal Tumors: A Review. J Mol Imag Dynamic 6:124. doi:10.4172/2155-9937.1000124

Copyright: © 2016 Abbas F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Introduction: Gastrointestinal tumors are rarest of the gastrointestinal tumors. They arise from interstitial cells of Cajal. Objective: To review the current diagnosis and management of gastrointestinal tumors. Material and methods: Pubmed was searched for ‘gastrointestinal tumors’ and relevant studies used for this review. Conclusion: GISTs are one of the rarest tumors of GI tract. They are more common in middle aged males. Diagnosis involves radiology, histopathology and immunohistochemistry. Treatment is surgical and chemotherapy.

Keywords

Gastrointestinal stromal tumors; DOG 1; C-kit

Abbreviations

GIST: Gastrointestinal Stromal Tumors; PDGFRA: Platelet-Derived Growth Factor Receptor Alpha; NF1: Neurofibromatosis 1

Introduction

Gastrointestinal Stromal Tumors (GISTS) are one of the rare tumors of Gastrointestinal Tract (GI tract). These non-epithelial tumors are most common mesenchymal tumors of GI tract. Earlier believed to have a neuronal or smooth muscle origin are now however known to arise from special cells of GI tract called Interstitial Cells of Cajal (ICCS). ICCS are also known as pacemakers of GI tract as they give signals to tract muscles for contraction. These muscles on contraction propel the food through the tract [1,2]. An incidence of 1.5/100,000/ year has been estimated for GIST [3]. The total number of cases in United States range from 4000 to 5000 (American Cancer Society).

Objectives

To review the current methods of diagnosis and management of GIST tumors and update the readers about recent research done on GISTs.

Material and Methods

A Pubmed search was made using the term ‘gastrointestinal tumors’ and relevant studies were used for collecting information regarding the current topic. The information was then presented under different subheadings for easier assimilation of the knowledge by the readers.

Discussion

GISTS are rare tumors accounting for less than 1% of all GI tumors. GISTS occur predominantly between 40-60 years of age. Median age of occurrence is around 60 years [4]. GISTS are more common in males. In pediatric age group GISTS have been found predominantly in girls. GI tract is the most common location contributing to more than 80% of GISTS. 50-70% of GI tract GISTS are located in stomach [5]. In GI tract these arise from the layer muscularis propria [6]. Other sites include retroperitoneal space, omentum and pelvis minor. Genetic syndromes have been associated with GISTS. These include familial GIST, Neurofibromatosis type 1 and Carney Stratikis syndrome [7]. GISTS are associated with gene mutations. In 85% cases of GIST, tumor behavior is driven by kit mutations. C-kit receptor tyrosine kinase gene encodes for a transmembrane receptor. This is a growth factor receptor called stem cell factor. Mostly there is mutation in the C-kit gene itself; however some time there could be a defect in constitutive activity of C-kit enzyme pathway. Mutations occur in exons (9-17) of this gene. 10% GISTS have PDGFRA gene mutation, which is again a receptor tyrosine kinase gene. Mutations are seen in exons 12, 14 and 18 of PDGFRA gene. Rarely BRAF kinase mutations can be seen. The inhibition of this Tyrosine Kinases (TK) has revolutionized the therapy of these tumors as specific targeted treatment with TK inhibitors is now available [8-10]. The exquisite sensitivity of these tumors to the novel tyrosine kinase receptor inhibitors imatinib, mesylate and sunitinib has significantly changed management and prognosis of these tumors [11]. Some GISTS do not harbor C-kit or PDGFRA mutations, these carry wild type of mutations in all hot spots of C-kit and PDGFRA genes. Recent discoveries have highlighted mutations in though less common genes including NF1, SDH, CD34, nestin, SMA, caldesmon, calponin, vimentin and embryonic smooth muscle actin. 7 CD34 is ligand of tyrosine kinase encoded by C-kit. DOG1, a novel protein encoded by ANO1 (Anoctatmin), is a calcium chloride regulated channel. It is also known as transmembrane protein 16A (TMEM 16A.12It). (DOG1) is a recently described mouse monoclonal antibody, and has more specificity and sensitivity compared with C-kit and CD34 for GISTS. It is expressed in C-kit positive as well as C-kit negative GIST [12].

Diagnosis

The dictum for GIST diagnosis involves clinical suspicion followed by radiological detection, histopathological correlation and finally immunohistochemical confirmation. Most GISTS are discovered when they are less than 4 cm in size and are asymptomatic. Symptomatic GISTS mostly present with GI bleeding, fullness, pain abdomen, palpable masses and obstructive features [13]. Metastasis is seen in liver in 65% of cases and in omentum in 25% of cases. Nodal metastases are rare in GIST [14]. Clinically age of the patient, family history and medical condition should be taken into consideration.

Imaging

On X-ray stomach GIST appears as a radioopaque shadow and intestinal gist shows an obstructive pattern. Small sized tumors have been accidently discovered on endoscopy as submucosal process or masses. On barium studies GISTS appears to be like an intramural mass having smooth borders forming right or obtuse angles with adjacent bowel. Bull’s eye and target leision signs appear suggesting ulcerated mucosal surface. As the tumor grows it becomes an exophytic mass projecting into abdominal cavity. It can undergo necrosis and cavitation if it loses blood supply, eventually it will ulcerate and communicate with bowel lumen to form air fluid levels on barium studies [15,16].

Role of CT scan, MRI, PET scan

CT scans help to find the location, size and extent of spread of tumors. On contrast enhanced CT small tumors appear as homogenous intramural masses. Large tumors appear heterogenous with peripheral enhancement and low attenuation centre [17]. MRIs can also help to find the extension and spread of tumors. A useful diagnostic method is f-fluoro-deoxyglucose positron emission tomography [18]. It is used in rapid assessment of tumor spread, response to imatinib and development of imatinib resistance in patients [19]. In this method FDG sugar is injected into the blood and after half an hour the scan is done to see the radioactivity of the body [20].

Histopathology

Once a tumor is detected on imaging it’s difficult to distinguish between GIST, other malignancies and some focus of infection so biopsy is needed to reach the final diagnosis. Endoscopic biopsy, Fine Needle Aspiration (FNA) biopsy or surgical biopsy can be done to find out the histopathological diagnosis of GISTS.

Macroscopically

Grossly the tumor appears like an exophytic serosal nodule ranging in size from 1-2 cm in asymptomatic cases to more than 20 cm in large symptomatic high risk tumors [21]. Specimen is homogeneous, grey white, nodular or lobulated, cystic, trabeculated with gelatinous areas of hemorrhage and necrosis.

Microscopically

Three types have been seen in GISTS. 70% of GISTS are spindle cell tumors. Spindle cells are uniform elongated cells having eosinophillic cytoplasm and ill-defined cell borders and uniform ovoid nuclei. 20% of tumors are of epitheloid morphology with cells having pale eosinophillc to clear cytoplasm and round nuclei. Rest of the 10% tumors are of mixed spindle cell and epitheloid morphology.

Immunohistochemistry

Molecular studies have shown that most GISTS express C-kit and PDGFRA having gain of function mutation in these genes. Over 90% of GISTS are positive for C-kit. Expression of C-kit by GISTS has revolutionised the treatment using TK inhibitors like imatinib mesylate and sunitinib. Kinase mutation status helps to predict if the tumor can respond to TK inhibitors and prognosis after tumor resection. CD117 exhibits a diffuse, focal or mixed staining pattern. Other tumors which show CD117 positvity include kaposis sarcoma, melanoma and deep fibromatosis, so a more specific and sensitive marker should be present for diagnosing GIST. These markers can diagnose CD117 negative GIST and include DOG 1, nestin, protein kinase c, theta carbonic anhydrase II and succinate dehydrogenase. DOG1 is a new promising marker of GIST being more sensitive and specific than C-kit. DOG1 expression is not affected by treatment with TK inhibitors which make CD117 fade and eventually turn negative after prolonged treatment. It stains about one third of kit negative GISTS. In a study conducted by West et al; immunoreactivity for DOG1 GISTs samples was 97.8% reactive [12]. DOG1 antibody stains tumour cells and interstitial cells of cajal with no background staining. It is used as a specific marker of GIST [22]. However in desmoplastic melanomas, synovial sarcomas, leiomyosarcomas, basalcell carcinomas, hepatocellular carcinomas, adenoid cystic carcinomas, bowel adenomas, esophageal, squamous cell carcinomas, gastric carcinomas and uterine-type retroperitoneal leiomyomas it shows spriadic positivity. However, given that between 36% and 50% of CD117-negative tumors are DOG1-positive; this antibody should be included in routine histochemical diagnosis of GISTs [23]. Together C-kit and DOG1 can define GISTS in 99% of cases.

An early marker of GISTS was CD34 but it’s less sensitive and specific as compared to kit and DOG1. It’s expressed in 50 to 80% of GISTS. Other markers include Ki 67, s-100 (positive in 6-28%), smooth muscle actin which is positive in 20-40% of cases, desmin(positive in 4-5% of cases)and vimentin. These markers however are less specific for GIST [24].

Prognosis and Treatment

Prognosis of GISTS depends on size of tumor and number of mitosis per 50 high power fields and depth of mucosal infiltration (Table 1) [25]. C-kit mutation in GISTS are associated with poor prognosis [26]. In a study it has been found that deletions in kit codon 557/558 had worse prognosis than any other kit exon 11 mutations or deletions not involving codon 557/558 better Five year survival was seen in kit exon 11 point mutations or duplications [27]. DOG1 expression it is an important diagnostic tool in patients with kit negative [28]. The main goal of treatment is complete surgical resection of the tumor with negative resection margins and preservation of an intact pseudocapsule [29]. Chemotherapy in GISTS is given with tyrosine kinase inhibitors like imatinib or sunitinib. These agents act by blocking signals via C-kit and PDGFRA receptors. It is given preoperatively as induction therapy for irresectable or borderline resectable tumors or as adjuvant therapy in in patients having high risk of recurrence and metastatic disease. Sunitinib is second line therapy which is given in imatinib resistant cases.

    Risk of Progressive Disease (%)  
Mitotic Index Size, cm Gastric Duodenum Jejunum and Ileum Rectum
<5/50-HPFs <2cm None  None None None
<5/50-HPFs >2 to <5 cm Very Low(1.9) Low (8.3) Low (4.3) Low (8.5)
<5/50-HPFs >5 to <10 cm Low (3.6) Insufficient data Moderate (24) Insufficient data
<5/50-HPFs >10 cm Moderate (12)  High (34) High (52) High (57)
<5/50-HPFs <2 cm None† Insufficient data High† High (54)
<5/50-HPFs >2 to <5 cm Moderate (16) High (50) High (73) High (52)
<5/50-HPFs >5 to 10 cm High (55) Insufficient data High (85) Insufficient data
<5/50-HPFs >10 cm High (86) High (86) High (90) High (71)
*Adapted from Miettinen M, Lasota RJ. Gastrointestinal stromal tumours: pathology and prognosis at different sites. Semin Diagn Pathol. 2006;23:70–8326.
Abbreviations: GIST: Gastrointestinal Stromal Tumor ; HPF: high-power field.

Table 1: Risk stratification of primary GISTs based on mitotic index, tumour size, and anatomic site.*

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  1. Ayman
    Posted on Oct 05 2016 at 7:31 pm
    The article provides useful information and will help in the improving the treatment practices in persons with special needs. Discussion may be included, as a form to demonstrate applications in the clinical practice.
 

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