alexa GSK3β and its Role in Sepsis | OMICS International
ISSN: 2155-6121
Journal of Allergy & Therapy
Like us on:
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

GSK3β and its Role in Sepsis

Tomeka Subber and Yutong Zhao*

Department of Medicine, The University of Pittsburgh, Pittsburgh, Pennsylvania, USA

*Corresponding Author:
Yutong Zhao
Department of Medicine, The Acute Lung Injury Center of Excellence
The University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
Tel: 412-648-9488
E-mail: [email protected]

Received date: March 19, 2017; Accepted date: March 21, 2017; Published date: March 28, 2017

Citation: Subber T, Zhao Y (2017) GSK3β and its Role in Sepsis. J Allergy Ther 8:e119. doi: 10.4172/2155-6121.1000e119

Copyright: © 2017 Subber T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Journal of Allergy & Therapy


Sepsis is a leading cause of critical illness and death worldwide. The most recent consensus statement defines this complex clinical syndrome as “life-threatening organ dysfunction caused by a dysregulated host response to infection” [1]. Despite clinical studies that have led to improved quality of care in the management of sepsis, mortality remains high [2]. An unmet need in the field is the development of pharmacologic interventions that target aberrant pathways that cause tissue injury in sepsis. Glycogen synthase kinase-3β (GSK3β), while initially identified in studies of glucose metabolism, has critical roles in apoptosis, cell proliferation, and inflammation (Figure 1). Over the past decade, studies have shown many inflammatory pathways to converge on GSK3β. In experimental models of sepsis, inhibition of GSK3β kinase activity decreases severity of organ dysfunction and reduces mortality. These studies suggest that GSK3β is a promising therapeutic target in the treatment of sepsis.


Figure 1: GSK3β modulates multiple biological functions.

GSK3β as a Central Kinase in Inflammation

GSK3β is a serine-threonine kinase that was first characterized in glucose metabolism [3]. It shares 85% homology with GSK3α, but the C-terminal 76 residues only share 36% identity [4]. Both GSK3α and GSK3β have high basal constitutive activity, but they have divergent functions beyond metabolic pathways [5]. Phosphorylation of Ser9 by upstream kinases such as PI3K-Akt inhibits GSK3β kinase activity (Ser21 on GSK3α), while phosphorylation of Tyr216 (Tyr279 in GSK3α) increases activity (Figure 2) [6,7]. Homozygous GSK3β -/- mouse embryos die from massive hepatocyte apoptosis and liver degeneration [8]. This phenotype is not rescued by expression of GSK3α. Fibroblasts from GSK3β -/- were more sensitive to TNFα- induced apoptosis, and this effect was reversed with neutralizing TNFα antibodies or exogenous expression of GSK3β. Later studies showed parallels between the GSK3β -/- and RelA -/- phenotypes, raising more questions about the role of GSK3β in the regulation of NFκB pathways. GSK3β is required for efficient DNA binding of p65 and expression of IL-6 and MCP-1 in response to TNFα [9]. In contrast, a study by Vines et al. demonstrated that GSK3β had anti-inflammatory effects downstream from NFκB activation in human lung micro vascular endothelial cells after treatment with IL-1β and TNFα [10]. Beurel and Jope showed that STAT3 and STAT5 activation depend on GSK3β kinase activity, but STAT1 and STAT6 activation was not dependent on GSK3β [11]. Evidence suggests that GSK3β serves as a gatekeeper for NFκB activation, modulating expression of pro- and anti-inflammatory genes in a cell type and stimuli-dependent manner.


Figure 2: GSK3β activity is regulated by its phosphorylation state.

GSK3β Regulates Toll-Like Receptor Pathway Activation

In sepsis, pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs) propagate inflammation and tissue injury. These effects are mediated through pattern recognition receptors (PRRs) including toll-like receptors (TLRs). Martin et al. published the first study confirming a central role for GSK3β in TLR signaling [12]. LPS induces PI3K/Akt-mediated Ser9 phosphorylation and inhibition of GSK3β, allowing for augmentation of anti-inflammatory cytokines in human peripheral blood monocytes (PBMCs). Treatment with a GSK3β-specific inhibitor protected mice from endotoxin-induced sepsis and death. GSK3β inhibition in human PBMCs also reduced cytokine production in response to TLR2, TLR4, TLR5, and TLR9 ligands. Subsequent work identified mTORC1 as a negative regulator of GSK3β kinase activity through S6K, and this inhibition decreases LPS-induced proinflammatory cytokine production [13]. The field has since grown in the number of studies exploring how GSK3β regulates downstream signaling after TLR ligand binding. It has been found to be important in TLR3 signaling through its own K63-linked polyubiquitination and subsequent phosphorylation of TRAF6, allowing assembly of the TRIF complex that is required for TLR3 signaling [14]. In a mouse model infected with a live vaccine strain of Francisella tularensis, inhibitory Ser9 phosphorylation of GSK3β occurred in a TLR2-dependent manner in murine macrophages. GSK3β positively regulated NFκB and p65 DNA binding affinity while negatively regulating CREB DNA binding [15]. These effects were reversed by lithium treatment, a known inhibitor of GSK3β [6,16]. These early studies demonstrate a pivotal role of GSK3β in innate immune responses that contribute to the pathogenesis of sepsis [17].

GSK3β Inhibition is an Attractive Therapeutic Target in Sepsis

While GSK3β has been identified as a central kinase in inflammation, translational studies have emerged demonstrating benefits of GSK3β inhibition in animal models of sepsis and organ failure. In a rat model of sepsis, use of selective GSK3β inhibitors reduced LPS-induced liver injury and failure [18]. GSK3β inhibitors decreased severity of illness and improved survival in experimental models of acute lung injury [19,20]. GSK3β kinase activity has also been implicated in organ damage from liver ischemia, often a result of hypoperfusion as seen in septic shock [21,22]. In acute kidney injury (AKI), GSK3β induces apoptosis of renal epithelial cells after ischemic injury and stress [23]. Deletion of GSK3β expression or small molecule inhibition of GSK3β protects mice from AKI both in ischemia-reperfusion models and models of sepsis [24-26]. This data support a role for GSK3β in potentiating end organ damage in sepsis and is an attractive therapeutic target in both prevention of and treatment for organ dysfunction and failure.


GSK3β has become a focal point in research given its multifaceted role in inflammation. More studies are needed to understand how GSK3β activity is dysregulated in the pathogenesis of sepsis and in host immune responses to pathogens. The goal is to tailor therapies for GSK3β-mediated pathways that contribute to uncontrolled inflammation that accelerates organ dysfunction and failure in sepsis. It remains to be seen if the studies of small molecule inhibitors of GSK3β that have produced striking data in animal models can be translated to treating patients with this devastating illness.


Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Relevant Topics

Recommended Conferences

Article Usage

  • Total views: 774
  • [From(publication date):
    March-2017 - Apr 23, 2018]
  • Breakdown by view type
  • HTML page views : 701
  • PDF downloads : 73

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version