Heavy Metals Bioaccumulation by the Green Alga Cladophora herpestica in Lake Mariut, Alexandria, Egypt
Received Date: Nov 10, 2017 / Accepted Date: Jan 06, 2018 / Published Date: Jan 13, 2018
The present study was carried out to evaluate the concentrations of some trace metals in the water of Lake Mariut and their accumulation in tissues of the green alga (Cladophora herpestica). Sampling of water and algae were carried out at four different sites throughout the period from July to Sept 2017. The water of the collected samples was mostly alkaline (7.91-8.51) and showed a wide range of variation in Biological Oxygen Demand "BOD" (5.11-18.92 mg L-1), Chemical Oxygen Demand "COD" (21.36 - 81.36 mg L-1) and Electric conductance "EC" (918 -1827 μS Cm-1) Concentrations of heavy metals in algae were higher compared to their levels in water samples. The concentrations of the selected trace metals were ranged between 11.19 μg L-1 for Pb in water samples collected from site 1 to 271.36 mg Kg-1 for Cd in algae collected from site 3. The concentrations of trace metals exceeded the Egyptian Standards limits. The results showed that the water quality is unacceptable and should be improved for human consumption. Moreover, the study indicated that the high concentrations of trace metals in algal tissues demonstrated the utility of using Cladophora herpestica as a biomonitor of heavy metal contamination and bioavailability. The study included some recommendations aiming at improving both water quality and ecosystem of the lake.
Keywords: Trace metals; Lake Mariut; Bioaccumulation; Cladophora herpestica
Freshwater makes up about 3% of earth's water and is the source of virtually all drinking waters. Most of this water comes from reservoirs, rivers, streams and lakes; and these sources are vulnerable to pollution .
Rapid urbanization and industrialization have negative implications for water quality, where the industrial effluents directly discharge into the rivers without any consideration of the environment .
Algae are known to be good indicators of the extent of pollution in aquatic Ecosystems [3,4]. They have s biological characteristics making them a very good “early warning system” for pollution [1,4,5].
Most of the industries like textiles, pharmaceuticals, tanneries, paper mills and oil refineries are situated near the lake and discharge their effluents directly into it. Besides, huge quantities of solid waste, varieties of chemical fertilizers and residues of pesticides, petroleum products, launches, cargoes, boats, untreated sewage etc. regularly get dumped into the lake [2,4,5]. These pollutants hinder the growth of aquatic organisms . Thus, aquatic diversity is now in threatened condition. The primary productivity of the aquatic ecosystem is now adversely affected by various anthropogenic activities and dumping of industrial effluents and untreated sewage into the lake.
The current anthropogenic metal emissions have resulted in increasing the concentrations of water toxic elements to a hazardous level . The most important source of atmospheric lead is the combustion of fuel. Atmospheric deposition of heavy metal aerosols in the study area may originate from closed industrial activities such as waste incineration, heating plants and crude oil processing [8-10].
Green algae (Chlorophyta ) are responsible for most of the primary productivity in some water bodies through photosynthesis. In the absence of photosynthesis, the metabolic process consumes oxygen, causing oxygen depletion in the aquatic system.
The present study was carried out to investigate the water quality of Lake Mariut (pH, BOD, COD and EC) and to investigate the bioaccumulation of toxic trace metals (Cr, Cd ,Ni, V and Pb) by the green algae Cladophora herpestica , as well as the possibility of using this alga as a bio indicator.
Materials and Methods
The study area
Lake Maruit (31° 10′ N and longitude 29° 55′ E) is a small brackish water ecosystem in the northwestern corner of the Nile Delta of Egypt (Figure 1). Its maximum length is about 8 km and the depth of water fluctuates between 60 and 130 cm, with an average of 100 cm.
This lake suffers from draining of large amounts of municipal and industrial wastewaters.
Water and algal samples were collected from four different sites throughout the period from July to Sept 2017. Sampling sites were located at the northern, western, southern and eastern regions of the lake to cover all points of discharging municipal and industrial wastes.
Algae (Cladophora herpestica ) were collected in plastic bags containing seawater and transported immediately to the laboratory for analysis .
The pH of water samples was measured on spot using calibrated digital multi-meter (HACH, 51910), while Electric conductance (EC) was measured at 25°C in μS cm-1, using an electric conductivity meter (HANNA, HI 8033). Chemical Oxygen Demand (COD) was measured by closed reflux colorimetric method using Colorimeter (HACH, DR/ 890), while Biological Oxygen Demand (BOD) was done by 5-days incubation, 20°C method 
Extraction and elemental analysis
Air-dried algal samples were wet-digested in a mixture of HNO3 and HClO4 (4:1, v/v), and the concentrations of Cr, Cd, Ni, V and Pb were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES) using an IRIS Intrepid II XSP instrument. All chemicals used were of analytical reagent grade . A six point calibration procedure was applied with multi-element calibration solution (Merck ICP multi-element standard solution IV) .
Bioaccumulation coefficients in correlated algae / waste water environments have been calculated to qualify the toxic elements accumulation efficiency of Cladophora herpestica by dividing metal content in algal biomass over metal content in Lake water.
Results and Discussions
Table 1 shows the parameters of water quality in the lake. pH of water was mostly alkaline (7.91–8.51).
|(mg L-1)||(mg L-1)||(µS Cm-1)|
Table 1: Physiochemical parameters of water quality at the selected sampling sites.
Biochemical oxygen demand (BOD) is a measure of the oxygen in the water that is required by the aerobic organisms. High BOD levels indicate lower in dissolved oxygen (DO), because the oxygen that is available in the water is being consumed by the bacteria leading to the inability of fish and other aquatic organisms to survive in the water bodies [12,13]. The values of BOD of the collected samples were 5.11, 18.92, 11.65 and 8.92 mg L-1 for sites 1, 2, 3 and 4 respectively (Table 1). The permissible limits of BOD 0.2, 3.0, 6.0 and 10.0 mg L-1 for drinking water, recreation, fish culture and irrigation, respectively .
Electric conductivity (EC) showed a wide variation from 918 μS cm-1 to 1827 μS cm-1. Although it is not a human or aquatic health concern; but it can serve as an indicator of other water quality parameters. The acceptable range of EC for irrigation, recreational and aquaculture water are 750, 500 and 800-1000 μS cm-1, respectively . Therefore EC of water exceeded permissible levels.
Table 2 shows the concentrations of trace metals in water samples and algal tissues collected from the different sampling sites in the lake.
|Element||Sampling site 1||Sampling site 2||Sampling site 3||Sampling site 4|
Table 2: Concentrations of different elements in water samples (μg L-1) and algal tissues (mg Kg-1) at the four different sampling sites (n=15).
The northern site (site 1) had the lowest concentrations of Cr, Cd, Ni, V and Pb (32.17, 26.11, 98.31, 147.31 and 11.67 μg L-1, respectively), while the southern site (site 3) had the highest concentrations of these metals (88.74, 48.21, 181.14, 101.16 and 23.26 μg L-1, respectively). This was reflected in higher concentrations of these metals in algal tissues collected from these two sites. The other two sites had intermediate concentrations.
The Southern site (3) is located near the industrial zone and most of by sewage and other inputs are discharged there. Municipal and domestic wastes as well as effluents from industries; slaughterhouses are dumped in the lake daily. Large volumes of wastes, produced daily from highly populated urban areas around the lake and, are carried by drainage systems and finally find their way into the lake .
Cladophora herpestica abundantly growing on the surface of the Mariut Lake accumulates heavy metals from the aquatic environments. The next goal of our study was to investigate the heavy metal occurrence in both algae and water environment.
|Element||Site 1||Site 2||Site 3||Site 4|
Table 3: Bioaccumulation coefficients in algae.
The magnitude of Cr, Cd and Pb metal bioaccumulation was found to be higher (175, 186 and 181 times, respectively) in samples collected from northern site (site 1) when compared with metals collected from algal tissues from different sites (Table 3). This high accumulative ability indicates that bioavailability of these metals at this site; especially it has the lowest concentrations of thee metals in its water samples. This is in agreement with the results of Aydoğan et al.  who reported bioaccumulation of Pb, Ni, Cu, and Cr heavy metals in two bryophyte species in Turkey, at similar levels of the present study.
Algal tissues collected from southern site (site 3) had the highest concentrations of V (256 times) when compared to other three sites (143, 174 and 117 times at sites 1,2 and 4, respectively), while it has the lowest concentrations of Cd, and Pb.
There was no significant (p=0.05) differences in bioaccumulation of Pb in tissues collected from different sources indicating lower concentration and water and consequently, lower bioavailability. These variations in bioaccumulation factors may be due to dilution of the lake towards the north [4-6].
Water is the most valuable and vital resource for sustenance of life. Unfortunately, agricultural, domestic and industrial wastes dumped into Lake Mariut over the years, which caused its deterioration and lower water quality. The results also showed a poor water quality.
The current waste management in Egypt is based on independent, self-solid waste incineration, including combustion of the bankdischarged algae . Most the produced hazardous waste (sewage sludge, petroleum contaminated soil and concrete material) has been incinerated and dumped in the lake. This incineration causes air pollution and worsens the quality of the Ecosystem. However, some alternative waste management scenarios, e.g. composting, especially for sludge and algae, have been recommended for the future, to relieve the present incineration of solid waste, which does not satisfy the updated criteria of air pollution control and investment costs for the construction of new incinerators are extremely high.
Cladophora herpestica is dominating and abundantly growing on the surface of the Maruit Lake, it accumulates residual nutrients and heavy metals from both the aquatic and atmospheric environment .
The high bioaccumulation abilities of Cladophora algae for selected metals have been confirmed. We suggest composting, especially for sludge and algae as an alternative waste management scenario; especially the algal biomass is recommended on the basis of the analyzed and acceptable toxic metal content for compost processing.
We are indebted to anonymous Reviewers for their invaluable comments.
- Islam SMD, Huda ME (2016) Water Pollution by Industrial Effluent and Phytoplankton Diversity of Shitalakhya River, Bangladesh. J Sci Res 8: 191-198.
- Haiba NS (2017) Polycyclic Aromatic Hydrocarbons (PAHs) in the River Nile, Egypt: Occurrence and Distribution. Polycyclic Aromatic Compounds 36: 1-12.
- Qari H, Hassan IA (2017) Bioaccumulation of PAHs in Algae Collected from a Contaminated Site at the Red Sea, Saudi Arabia. Polish J Environ Studies 26: 435-439.
- Qari H, Hassan IA (2017) Oxidative Stress of Bioaccumulation of Cu, Cr and Ni in Ulva Reticulate in the Red Sea of Jeddah. J Pollut 1: 101.
- Khalil MT, Koussa AA (2013) Ecological Studies on the Zooplankton Community of Lake Maruit, Egypt. The Global J Fish Aqu Res 5: 295 – 311.
- Soylu EN, Gönülol A (2003) Phytoplankton and seasonal variations of the River Yeşilırmak, Amasya, Turkey. Tur J Fish Aqu Sci 3: 17-24.
- Odum EP, Barrett GW (2008) Fundamentals of ecology (5thedn). Thomson Brooks, Australia.
- Chmielewská E, Medved J (2001) Bioaccumulation of Heavy Metals by Green Algae Cladophora glomerata in a Refinery Sewage Lagoon. Croat Chem Acta 74: 135-145.
- Boryło A, Nowicki W, Skwarzec B (2013) The Concentration of Trace Metals in Selected Cultivated and Meadow Plants Collected from the Vicinity of a Phosphogypsum Stack in Northern Poland. Pol J Environ Stud 22: 347-356.
- Whitton BA, Say PJ, Wehr JD (2000) Use of Plants to Monitor Heavy Metals in Rivers. In: Say PJ, Whitton BA (eds.), Heavy Metals in Northern England: Environmental and biological aspects.
- Hassan IA, Basahi JM (2013) Assessing Roadside Conditions and Vehicular Emissions using Roadside Lettuce Plants. Pol J Environ Stud 22: 387-393.
- Abowei JFN, Ezekie EN, Hansen U (2012) Effects of Water Pollution on Phytoplankton Species Composition in Koluama Area, Niger Delta Area, Nigeria. Int J Fish Aqu Sci 1: 134-139.
- D’Costa PM, D‘Silva MS, Naik RK (2016) Impact of Pollution on Phytoplankton and Implications for Marine Econiches. In: Naik MM, Dubey SK (eds.), Marine Pollution and Microbial Remediation. Springer Science Business Media, Singapore. pp: 205-222.
- Aydoğan S, Erdağ B, Aktaş LY (2017) Bioaccumulation and Oxidative Stress Impact of Pb, Ni, Cu, and Cr Heavy Metals in Two Bryophyte Species, Pleurochaete squarrosa and Timmiella barbuloides. Turk J Bot 41: 464-475.
- Soylu EN, Gönülol A (2003) Phytoplankton and Seasonal Variations of the River Yeşilırmak, Amasya, Turkey. Turk J Fish Aquat Sci 3: 17-24.
Citation: El Maghraby DM, Hassan I (2018) Heavy Metals Bioaccumulation by the Green Alga Cladophora herpestica in Lake Mariut, Alexandria, Egypt. J Pollut 1: 102.
Copyright: © 2018 El Maghraby DM, et al. This is an open-access article distributed under the terms of the creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.