Davis Heart and Lung Research Institute and Division of Cardiovascular Medicine, Wesxner Medical Center, The Ohio State University, Columbus, OH 43082, USA
Received Date: November 02, 2014; Accepted Date: November 04, 2014; Published Date: November 06, 2014
Citation: Ilangovan G (2014) High Level of Heat Shock Protein 25 (Hsp25) Expression in Atherosclerotic Lesions. J Cytol Histol 5:i106. doi: 10.4172/2157-7099.1000i106
Copyright: © 2014 Ilangovan G. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Confocal microscopic analysis of Immunofluroscence of triple stained aortic atherosclerotic lesions in low density lipids receptor knock out (LDLr–/–) mouse. Purpose of this study was to analyze a small heat shock protein, namely Hsp25, expression in atherosclerotic lesions. LDL-/- mice were fed with high cholesterol containing western diet to create aortic lesions. Descending aorta was isolated from these mice and stained with florescent dye tagged antibodies: GREEN channel actin; BLUE channel DAPI; and RED channel Hsp25. Actin in intima is clearly seen without overlap with red color. However in lipid rich lesions, yellow colored blocks are observed, as indication these smooth muscle layers containing the lipid cores. The yellow blocks indicate the high in Hsp25, in addition to high red fluorescence likely from not cellular structure and perhaps the lipid rich area. This is key evidence showing high Hsp25 expression in atherosclerotic lesions (Figure 1).