alexa High Performance Liquid Chromatographic Determination of Naproxen in Prepared Pharmaceutical Dosage Form and Human Plasma and its Application to Pharmacokinetic Study | Open Access Journals
ISSN: 2157-7064
Journal of Chromatography & Separation Techniques
Like us on:
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

High Performance Liquid Chromatographic Determination of Naproxen in Prepared Pharmaceutical Dosage Form and Human Plasma and its Application to Pharmacokinetic Study

Saiqa Muneer1,2*, Iyad Naeem Muhammad1 and Muhammad Asad Abrar3

1School of Pharmacy, University of Queensland, Brisbane, Queensland, Australia

2The University of Lahore, Lahore, Pakistan

3The Islamia University of Bahawalpur, Bahawalpur, Pakistan

*Corresponding Author:
Saiqa Muneer
PhD Student, School of Pharmacy
University of Queensland, 20 Cornwall Street
Woollongabba, 4109, Brisbane
Queensland 4102, Australia
Tel: 0061469085227/469085227
Fax: 0469085227
E-mail: [email protected] (or) [email protected]

Received Date: May 27, 2017 Accepted Date: June 09, 2017 Published Date: June 14, 2017

Citation: Muneer S, Muhammad IN, Abrar MA (2017) High Performance Liquid Chromatographic Determination of Naproxen in Prepared Pharmaceutical Dosage Form and Human Plasma and its Application to Pharmacokinetic Study. J Chromatogr Sep Tech 8: 369. doi: 10.4172/2157-7064.1000369

Copyright: © 2017 Muneer S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Journal of Chromatography & Separation Techniques


A simple, fast, economical and precise reverse phase liquid chromatographic method was developed, optimized and validated for quantification of naproxen sodium according to the standard guidelines. The separation of the analyte and internal standard was achieved over C-18 column using acetonitrile: water: glacial acetic acid as mobile phase in a ratio of 50:49:1 (v/v) in isocratic mode at a flow rate of 1.2 mL/min at a wavelength of 254 nm at ambient temperature. The retention time was found to be less than 10 minutes. The limit of detection (LOD) was 10 ng/mL and limit of quantification (LOQ) was 15 ng/mL. Naproxen sodium was extracted from biological samples by using acetonitrile as extraction solvent. The linearity was found to be 0.5 to 80 ppm. All the parameters were validated for accuracy, precision, linearity, sensitivity, reproductivity and stability. The method was successfully applied for the quantification of the naproxen sodium in animal and human plasma. Therefore, the method was found to be accurate, reproducible, sensitive, cost effective, less time consuming and can be successfully applied on routine analysis of naproxen sodium in pharmaceutical formulations and pharmacokinetic studies in human and animal models.


Naproxen sodium; RP-HPLC; Biological samples; Pharmacokinetics


Naproxen sodium is chemically sodium (2S)-2-(6- methoxynaphthalen-2-YL) propionate. (National Center for Biotechnology Information 2016). Naproxen is a propionic acid derivative that belongs to the aryl acetic group of non-steroidal antiinflammatory agents [1,2]. They act by inhibiting the prostaglandin synthesis thereby producing several side effects such as ulceration and gastrointestinal bleeding [3-5]. Although many high-performance liquid chromatographic methods have been developed for the estimation of naproxen sodium alone [2,5] or in combination for the simultaneous determination of naproxen with other drugs in different matrices. These methods involved derivatization and post-column photochemical reactions, mostly used buffer solution as mobile phase due to which the mobile phase becomes complex. But there is always a need of a meaningful cost-effective method for estimation of naproxen sodium because the present methods do not provide the sensitivity [1,2,5]. The aim of the present study was to develop a simple and effective RP-HPLC method for quantification of naproxen in pharmaceutical preparations [5-9] as well as in spiked human plasma and animal plasma (rabbits) [7,10,11] in a very short time and to validate that method according to ICH and FDA guidelines (Food and drug administration 2012; United states Pharmacopoeia 2007; International Conference on Harmonization (ICH) 2005;). The method was successful due to its suitability and reproducibility. This method is more precise, accurate, highly sensitive, rapid and cost effective when observed under different experimental conditions [12-15]. The dominance of the new assay for its intended use in the human pharmacokinetic study was upheld for its validation and applications over the other methods of naproxen estimation found in literature (Figure 1).


Figure 1: Chemical structure of Naproxen sodium.


Chemicals and reagents

Naproxen sodium was gifted by Life Pharma, Multan, Pakistan. Acetonitrile and water (HPLC grade) and glacial acetic acid was purchased from Merck (Germany). Other chemicals used were of analytical grade and Naproxen sodium tablet formulations used in this experiment were prepared during research work and a commercial brand was purchased from local market [16].


High performance liquid chromatographic system equipped with an auto sampler and UV visible detector was used for analysis. The data was recorded using Liquid chromatography software.

Chromatographic conditions

The liquid chromatograph is equipped with a 254-nm detector and a 4.6-mm × 15-cm column C-18 that contains 5-μm packing L1. The flow rate is about 1.2 mL per minute. Chromatograph of the standard preparation, and record of the peak responses as directed under procedure: the column efficiency, determined from the analyte peak, is not less than 4000 theoretical plates [17,18].

Mobile phase: A suitable mixture of acetonitrile, water, and glacial acetic acid (50:49:1) was prepared (Make adjustments if necessary. Increased resolution may be achieved by increasing the proportion of water in the mobile phase). The mobile phase was membrane filtered (pore size 0.45 μm), sonicated and degassed before use.

Solvent mixture: A suitable mixture of acetonitrile and water with a ratio (90:10) was prepared (Figure 2).


Figure 2: A chromatogram of naproxen sodium.

Preparation of assay

For estimation of naproxen sodium, twenty tablets of 275 mg naproxen sodium tablets were taken randomly, crushed and powdered and the average weight was calculated. The powder equivalent to 2.75 mg of naproxen sodium was taken and transferred to 100 mL volumetric flask and initially dissolved in 10 mL water, and sonicated for 10 minutes until the material was completely dispersed. Then added about 80 mL of acetonitrile, and sonicated for an additional 5 minutes. The flask was allowed to reach room temperature, diluted with acetonitrile to volume, and mix. Any insoluble matter was allowed to settle, then 1.0 mL of the clear supernatant was transferred to a 100 mL volumetric flask; diluted with mobile phase to volume, and mix [19,20].

Preparation of standard solution

An accurately weighed quantity of Naproxen WS was dissolved in solvent mixture to obtain a solution having a known concentration of about 2.5 mg per mL. Then transferred 1.0 mL of the resulting solution to a 100 mL volumetric flask, diluted with mobile phase to volume, and mix. This solution contains about 25 μg of Naproxen WS per mL.


Separately injected equal volumes (about 20 μL) of the Standard preparation and the Assay preparation into the chromatograph, recorded the chromatograms, and measured the responses for the major peaks. The relative retention times are around 0.6 for naproxen. The quantity, in mg, of Naproxen (C14H14O3) was calculated in the portion of tablets taken by the formula:

10C (RU/RS)

where, C is the concentration (μg per mL) of Naproxen WS in the Standard preparation, and RU and RS are the ratios of the response of the naproxen peak to the response of peak obtained from the Assay preparation and the Standard preparation, respectively.

The peak area was determined and plotted against concentration μg/mL in plasma. 20 μL was injected into chromatograph and each chromatogram is recorded. The results of calibration curve and system suitability are shown in Table 2 and Figure 3.


Figure 3: Calibration Curve for Naproxen Sodium.

Optimization of chromatographic conditions

Various experimental conditions such as different mobile phase, flow rate, detection wavelength and column over temperature were optimized for the analysis of RP-HPLC in isocratic mode. Good separation and shorter run time was obtained by the mobile phase used in varying concentrations of acetonitrile and water to adjust the flow rate to 1.2 mL/min where it gives the improvement in peak shapes of naproxen. Detection wavelength was also varied in the experiment to obtain different chromatograms to achieve better peaks and 254 nm was selected as optimized. Column C-18 well the naproxen by reverse phase chromatography.

Preparation of human plasma samples

Blood samples were collected in heparinized glass tubes from healthy volunteers in University of Lahore, Pakistan after obtaining the informed consent. They were then centrifuged at 3500 rpm for 10 minutes and then filtered through 0.45 μm membrane filter and the plasma is separated and stored at -20°C till analysis. At the time of analysis, the serum was spiked with the naproxen sodium drug solution in a ratio of 1:1 and diluted ten folds to obtain 100 μg/mL. 20 μL of reconstituted samples were injected into the HPLC-UV system [20,21].

Extraction: Naproxen sodium (internal standard) was extracted using acetonitrile ACN. The human plasma was then spiked with the internal standard of naproxen sodium (0.5 μg/mL). The samples were then vortexed for 2 mins and then centrifuged at 3500 rpm for 10 mins. The supernatant was collected and volume make up to 1 mL and 20 μL was injected directly into the system.

Preparation of rabbit plasma samples

Same procedure was adapted for rabbits and samples were collected at a predefined time from the rabbits in heparinized test tubes.

Method validation

The developed method was validated for specificity, accuracy, repeatability, precision, LOD, LOQ, robustness as per ICH guidelines.

Specificity: The difference between API and other excipients present in the sample is called specificity. The specificity was determined by injecting the excipients and standard solution of naproxen sodium.

Accuracy: Accuracy is defined as the nearest of the nominal and the actual results. The accuracy is expressed as percentage recovery of the analyte recovered by the assay. Accuracy was determined by the applying the proposed method to different concentrations of drug product mixtures. The injections were taken in triplicate and mean peak area is taken to calculate the concentration.

The accuracy of the proposed method is calculated by the following equation


The average accuracy was 98.5% Mean recoveries for naproxen sodium for the specific formulation are shown in table. The results show that are the parameters were within the expected range (Table 1).

Injected conc. (µg/mL) Recovered conc. (µg/mL) % Recovery
107 105.82511 98.9
120 119.1235 99.26
230 224.4162 97.56

Table 1: Accuracy (% recovery) of Naproxen.

Repeatability: The repeatability was done on 3 replicates of each concentration of the standard solution.

Linearity: The linearity plots were obtained over a concentration range of 15 μL to 40 μL to encompass the expected concentration in measured samples. The average peak areas were plotted against respective concentration. The linearity of the proposed method was studied by using the calibration curves (Figure 1) for the determination of the coefficient of correlation and intercept values (Tables 2 and 3).

Conc. of Standard Standard ID Peak Area Mean
15 µg/mL A A1 2942222 2941321.67
A2 2936694
A3 2945049
20 µg/mL B B1 3513865 3528324.00
B2 3524865
B3 3546242
25 µg/mL C C1 4098615 4091748.67
C2 4087587
C3 4089044
30 µg/ml D D1 4630074 4650964.00
D2 4659300
D3 4663518
35 µg/mL E E1 5104615 5197315.00
E2 5244524
E3 5242806
40 µg/mL F F1 5801346 5784360.00
F2 5753372
F3 5798362

Table 2: Linearity Details of Naproxen sodium.

Standard ID Concentrations Peak area (Mean)
A 15 µg/mL 2941321.67
B 20 µg/mL 3528324.00
C 25 µg/mL 4091748.67
D 30 µg/mL 4650964.00
E 35 µg/mL 5197315.00
F 40 µg/mL 5784360.00

Table 3: Linear regression data for naproxen sodium calibration curve.

Limit of Detection and Limit of Quantification: The limit of detection (LOD) was and limit of quantification (LOQ) were determined as per ICH guidelines. The limit of detection (LOD) was 10 ng/mL and limit of quantification (LOQ) was 15 ng/mL (Table 4).

Spiked conc. Conc. observed Accuracy (%RSD)
20 20.381 101.90 1.48
10 10.202 102.02 2.72
5 4.93124 98.62 2.06
1 1.014 101.4 1.35
0.5 0.49878 99.75 0.21

Table 4: Determination of naproxen in tablet dosage form by suggested HPLC method.

Precision: Precision was determined through both reproducibility and repeatability. It is defined as the degree of agreement among individual test results where the method is applied repeatedly to multiple readings. Precision is determined by interday and intraday precision. Precision is done by applying the data of three different concentrations on consecutive three days throughout the linearity range thrice in a day. The relative standard deviation for the response factor was calculated. The %RSD value measured during assessment of precision was <2% for naproxen sodium indicating that the method is precise as per ICH guidelines (Tables 5 and 6).

Spiked conc. Conc. observed Accuracy (%RSD)
20 20.212 101.06 1.4
10 10.43 104.3 2.3
5 4.80 96 1.89
1 1.23 123 1.24
0.5 0.54 108.2 2.15

Table 5: Determination of naproxen in human plasma by suggested HPLC method.

Spiked conc. Conc. observed Accuracy (%RSD)
20 20.421 102.1 2.1
10 9.464 94.6 0.87
5 5.023 100.46 1.02
1 0.985 98.5 1.13
0.5 0.49 98 0.912

Table 6: Determination of naproxen in rabbit plasma by suggested HPLC method.

Sample analysis: To quantify the amount of drug in the finished tablet dosage form, the sample solution of naproxen sodium was analyzed by the proposed method. The contents were determined by the use of calibration curves and the formula is given by,


Where, Y=peak area of the analyzed sample; m=slope of the analyzed sample; x=concentration of the analyzed sample and c=intercept of calibration curve.

System suitability: System suitability was conducted in order to confirm the effectiveness of the chromatographic system. Fr this purpose, different suitability parameters such as retention time, tailoring factor, capacity factor (k), selectivity factor (α), resolution, number of theoretical plates were checked by repeatedly injecting the solution of naproxen sodium. The values are given in Table 7.

Validation parameter Naproxen
Recovery (%) 98.56
Repeatability (%RSD) 1.564
Precision range 0.87-2.87
Interday (n=3) 1.79
Intraday (n=3) 1.95
Limit of detection (ng/mL) 10
Limit of quantification (ng/mL) 15

Table 7: System suitability parameters.

Robustness: The robustness of the method was determined by minor deliberate changes in experimental conditions such as column oven temperature (± 2°C), flow rate of mobile phase (± 0.2 mL/min), composition of mobile phase (± 1). The data obtained after the minor changes in the experimental conditions did not affect the recoveries, peak area and the retention time which showed that the method is robust.

System stability: Stability was tested in an auto sampler for 0, 6, 12 and 24 hr and triplicates of low and high control samples were analyzed and deviation was calculated. Long term stability was tested for two weeks by taking low and high-quality control samples in triplicate. The mean concentration of sample was taken and compared with the samples taken at 0 hr [22] (Figure 4).


Figure 4: Plasma concentration time plot in different healthy volunteers following oral administration of prepared naproxen sodium 275 mg tablets.

Application of the Methods

This method was a part of an extensive research, “Establishment of in-vitro in-vivo correlation (IVIVC) studies for developed naproxen sodium tablet formulations”. The method was successfully applied to the in-vitro in-vivo evaluation of the pharmaceutical formulations of naproxen in animal models as well as in human plasma. In pharmaceutical development, it is important to employ in-vitro release to select formulations that can provide the adequate therapy. Drug release kinetics provide valuable data on the structural behavior of the drug and its release pattern. The developed method was successfully employed for in-vitro evaluation and pharmacokinetic data of naproxen sodium following IV administration of this drug. The drug content of each sample was determined and the pharmacokinetic parameters were determined by the use of the software.


A reverse phase HPLC method of Naproxen sodium has been developed in tablet dosage form and validated as per ICH guidelines, USP and FDA by using mobile phase of acetonitrile and water in a ratio of 50:49:1 at pH 7.8 using 4.6 mm × 15 cm column that contains 5 μm packing L1 at ambient temperature. The flow rate was 1 ml/ min and the eluent was determined at a wavelength of 254 nm. The retention time of naproxen was 4.699 min (Figure 2). Precision was reflected by mean %RSD of 1.564 which showed the sensitivity of the developed method. A simple extraction procedure was implemented resulting in good recovery of both drug and the internal standard. All the analyte was separated and well resolved from less than 7 minutes. Various experimental conditions such as column over temperature, pH of the mobile phase, detector wavelength, mobile phase ratio and flow rate were optimized for quantitative analysis of naproxen sodium in spiked human plasma and pharmaceuticals and biological samples. The current method is simple, economic, accurate, precise, specific, and rapid and will be conveniently applied for routine analysis in pharmaceutical dosage form, spiked human plasma and biological samples with slight modification in extraction procedures.


Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Recommended Conferences

Article Usage

  • Total views: 206
  • [From(publication date):
    June-2017 - Jul 21, 2017]
  • Breakdown by view type
  • HTML page views : 188
  • PDF downloads :18

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version