alexa Histological Studies of the Effects of Aeromonas salmonicida on the Tissues in Brachymystax lenok | Open Access Journals
ISSN: 2157-7099
Journal of Cytology & Histology
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Histological Studies of the Effects of Aeromonas salmonicida on the Tissues in Brachymystax lenok

Shaowu Li1, Gefeng Xu2, Di Wang1, Zhenbo Mou1 and Tongyan Lu1*

1Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, PR China

2College of Animal Science and Technology, Northeast Agricultural University, Harbin, PR China

*Corresponding Author:
Tongyan Lu
Heilongjiang River Fisheries Research Institute
Chinese Academy of Fishery Sciences
Harbin, PR China
Tel: +86-451-84604803
E-mail: lutongyan@hotmail.com

Received Date: November 22, 2014; Accepted Date: November 24, 2014; Published Date: November 26, 2014

Citation: Li S (2014) Histological Studies of the Effects of Aeromonas salmonicida on the Tissues in Brachymystax lenok. J Cytol Histol 5:i107. doi:10.4172/2157-7099.1000i107

Copyright: © 2014 Li S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Introduction

Furunculosis caused by Aeromonas salmonicida occurred frequently in farm-raised Brachymystax lenok recently and affected the healthy development of cold-water fish aquaculture in China. In this study, the effect of a pathogenic strain A. salmonicida (LN1301) on four tissues (including liver, spleen, kidney and intestine) of fish was investigated. LN1301 was originally recovered from liver of diseased juvenile Brachymystax lenok with septicemia signs. Artificial infection proved that LN1301 was highly virulent to fish when challenged by wounded immersion or intraperitoneal injection. Healthy juvenile Brachymystax lenok (body weight of 90g-100g) were used for virulence assays and observation of histological changes. The experimental fish were randomly assigned into treatment group (n=3) and control group (n=3). The fish in the treatment group was intraperitoneal injected with LN1301 strain at a dosage of 1×106 CFU and the control fish received equal amounts of distilled water. The fish which exhibited clinical signs were then sacrificed and the tissues were taken carefully and fixed quickly into the Bouin’s solution for routine histological procedures. The results indicated that there were obvious histological changes in several tissues of LN1301 infected fish. As shown in Figure 1A, hepatic cells degenerated, cytoplasm became loosened, lots of lymphocytes infiltrated, amounts of erythrocytes increased in in liver. Regarding the spleen, parenchyma cells dissolved, nucleus enlarged and the cytoplasm became loosened (Figure 1B). In the kidney of diseased fish, epithelial cells appeared hydropic degeneration and separated from the basement membrane. Glomeruli was swelled and arranged in disorder with the increasing of the cystic cavity gap (Figure 1C). There was no obvious boundary among the layers of intestinal mucosa (Figure 1D).

cytology-histology-tissues

Figure 1: Photomicrographs of a section of four tissues of Brachymystax lenok challenged by A. salmonicida strain LN1301. A: Liver (400×); B: Spleen (400×); C: Kidney (200×); D: Intestine (400×).

Acknowledgement

This work was supported by grants from Special Scientific Research Funds for Central Non-profit Institutes, Chinese Academy of Fishery Sciences (2014A06XK05) and National "Twelfth Five-Year" Plan for Science & Technology (2012BAD25B10).

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