alexa HPLC Analysis of Penicillins in Veterinary Drugs | Open Access Journals
ISSN : 2153-2435
Pharmaceutica Analytica Acta
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HPLC Analysis of Penicillins in Veterinary Drugs

Samanidou V* and Evaggelopoulou EN

Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece

*Corresponding Author:
Samanidou V
Laboratory of Analytical Chemistry
Department of Chemistry
Aristotle University of Thessaloniki
54124 Thessaloniki, Greece
Tel: +30231997698
Fax: +302310997719
E-mail: [email protected]

Received date: March 18, 2015; Accepted date: March 19, 2015; Published date: April 26, 2015

Citation: Samanidou V, Evaggelopoulou EN (2015) HPLC Analysis of Penicillins in Veterinary Drugs. Pharm Anal Acta 6:e174. doi: 10.4172/2153-2435.1000e174

Copyright: ©2015 Samanidou V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Penicillins are beta (β)-lactam antimicrobial which are used against various organisms by inhibiting the synthesis of the peptidoglycan layer of bacterial cell walls. Broad-spectrum penicillins include ampicillin (AMP), penicillin G (PG), penicillin V (PV), oxacillin (OXA), cloxacillin (CLO) and dicloxacillin (DICLO). They act as bactericides being effective against Gram-positive and Gram-negative bacteria, but not very effective against Pseudomonas. Their chemical structures are shown in Figure 1.


Figure 1: Chemical structures of penicillins

An HPLC method was determined and validated for the simultaneous determination of penicillins in veterinary drugs, using an LC-10ADVP HPLC pump with Photodiode Array Detector, in compliance with data acquisition software LabSolutions-LC solutions by Shimadzu. Separation was performed on an Inertsil, C8,250 × 4 mm, 5 μm analytical column purchased from MZ-Analysentechnik (Mainz, Germany). Mobile phase consisted of CH3COONH4 0.05M and acetonitrile at a volume ratio 85:15 remaining isocratic for 5 min and changing to 40:60 in the next 20 min, was delivered at a flow rate of 1 mL/min. The method was applied to the analysis of commercially available pharmaceuticals.

Two veterinary drugs were analysed: ORBENIN L.A. by Pfizer Italia Srl (Latina, Italy), which contains 200 mg cloxacillin per 3 g of product and CLOXALENE PLUS by FatroS.p.A. (Bologna, Italy) containing 11 g ampicillin and 5 gdicloxacillin per 1 mL of product [1-4].

Accuracy of the method for analysed veterinary drugs ranged from -4-9.3% expressed as relative error. A representative chromatogram is shown in Figure 2.


Figure 2: Chromatogram of Cloxalene Plus injection (4.4 ng/μ Lampicillin (5.882 min) and 2 ng/μL dicloxacillin (21.603 min) in presence of lamotrigine as internal standard (14.133 min)


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