alexa Hypotony as a Hazard of Trabeculectomy with Mitomycin C | OMICS International
Journal of Eye Diseases and Disorders
Like us on:
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Hypotony as a Hazard of Trabeculectomy with Mitomycin C

Elizabeth McElnea*, Emer Henry and John Stokes

Ophthalmology Department, Waterford Regional Hospital, Ardkeen, Co. Waterford, Ireland

*Corresponding Author:
Elizabeth McElnea
Specialist Registrar in Ophthalmology MB BCh BAO
Mater Misericordiae University Hospital, Eccles Street, Dublin 7, Ireland
Tel: 00 353 86 8528074
E-mail: [email protected]

Received date: October 27, 2015; Accepted date: December 10, 2015; Published date: December 17, 2015

Citation: McElnea E, Henry E, Stokes J (2015) Hypotony as a Hazard of Trabeculectomy with Mitomycin C. J Eye Dis Disord 1:102. doi: 10.4172/jedd.1000102

Copyright: © 2015 McElnea E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Journal of Eye Diseases and Disorders


This report describes the difficulties faced in the management of a case of hypotony following trabeculectomy with mitomycin C in an attempt to emphasize the importance of this common complication and highlight the options available for its treatment.

Case Report

An eighty two year old Caucasian lady with primary open angle glaucoma attended eye clinic. She was using guttate latanoprost 50 g/ml, Brimonidine 2 mg/ml and combined Dorzolamide 20 g/ml and Timolol 5 mg/ml.

This lady was myopic with right eye manifest refraction spherical equivalent of -2.00 and left eye manifest refraction spherical equivalent of -8.00 dioptres. The left eye was amblyopic as a result of this anisometropia. This lady had had bilateral uncomplicated cataract extractions by phacoemulsification with intraocular lens implantation and subsequently bilateral neodymium yttrium aluminium garnet or Nd: YAG laser posterior capsulotomies.

This patient had a medical history of pulmonary tuberculosis and pulmonary fibrosis. She also had aortic valve regurgitation and osteoarthritis. Her regular medications were Digoxin 125 μg, Bumetanide 1mg and Lisinopril 1mg daily. She utilized supplementary oxygen for 16 hours daily.

At her examination the visual acuity from this lady’s right and better eye was 6/12 and that from her left eye 6/60 on a Snellen chart. Her intraocular pressures were 20 mmHg bilaterally. Both optic discs were cupped with approximate cup: disc ratios of 0.8. At Humphrey visual field testing progressive visual field losses was noted in this lady’s right eye as can be seen from Figure 1. Consequently glaucoma surgery was planned.


Figure 1: Humphrey Visual fields from our patient’s right eye showing glaucomatous visual field losses progressing over a three year period.

This patient subsequently underwent right trabeculectomy wherein three sponges soaked in mitomycin C 0.3 mg/ml were applied beneath a fornix based conjunctival flap for one minute prior to the creation of a 4 × 3 mm limbal based rectangular scleral flap and a 0.4 × 0.4 mm sclerotomy. The scleral flap was closed with two 10/0 Nylon sutures. The patient’s conjunctiva was noted to be thin and friable and was closed with considerable difficulty at the end of the procedure. This lady was admitted following her surgery, the poor vision from her left, un-operated eye, preventing her safe discharge back to her home.

On the first postoperative day this lady could perceive only hand movements from her right eye. She was noted to now fix with her amblyopic left eye. Her right anterior chamber was shallow. No bleb leak was identifiable. The bleb while shallow superiorly had extended significantly nasally, as can be seen from Figure 2. Choroidal effusions had developed in all four quadrants of the posterior segment. One such effusion can be seen at B-scan ultrasound in Figure 3.


Figure 2: Colour photograph of our patient’s right eye displaying an avascular and thin irregularly sutured conjunctiva at the 12 o’clock position (A) and a large nasal bleb (B).


Figure 3: B-scan ultrasound depicting a large superior choroidal fold in our patient.

A pressure pad was placed on this lady’s right eye for twenty four hours following which it was removed and a large diameter (18 mm) soft contact lens applied and treatment with guttate prednisolone acetate 1% and chloramphenicol four times daily and guttate atropine 1% twice daily commenced.

This lady subsequently showed signs of initially anterior and subsequently posterior chamber inflammation with anterior chamber cells and flare and vitreous cells and snowballs. Further, this patient’s anterior chamber failed to spontaneously reform and so was reformed in the operating theatre using an ophthalmic viscoelastic device six days postoperatively.

Unfortunately this lady’s eye again became hypotonous and the opinion of a glaucoma specialist from another unit was sought. It was advised that guttate prednisolone acetate should be administered only once daily and consideration be given to the placement of conjunctival compression sutures, the injection of autologous blood beneath the bleb or reformation of this lady’s anterior chamber using sulphur hexafluoride (SF6) gas.

Following the reduction in this lady’s steroid therapy as suggested the large nasal bleb began to decrease in size. This patient’s intraocular pressure began to increase and her visual acuity improved. This lady was discharged to her own home when the visual acuity from her right eye reached 6/12 on a Snellen chart exactly one month after her initial admission.

Unfortunately two months later she returned to our eye casualty service describing a reduction in visual acuity from her right eye. At her examination the vision from this eye was 6/120 and she was noted to again preferentially fix with her ambylopic left eye. The intraocular pressure of the right eye was 4 mmHg. An increase in vitreous chamber activity since the time of her last review was noted. Prednisolone 60 mg daily was prescribed and tapered by 10 mg every week thereafter. Esomeprazole 40 mg, calcium 500 mg and vitamin D3 400 international units were also commenced.

This lady’s intraocular inflammation subsided, and her intraocular pressure and visual acuity increased. At this lady’s last review her right visual acuity was 6/12 as pre-operatively and her intraocular pressure 18 mmHg.


Trabeculectomy creates a fistula for the drainage of aqueous humour from the anterior chamber to the sub-conjunctival space from where it is subsequently absorbed by the surrounding tissues. It is the most commonly performed operation for glaucoma [1]. Trabeculectomy has been found to achieve desired intraocular pressures and to prevent the progression of visual field losses in 80%-90% of those undergoing the procedure [2].

The addition of the use of the anti-fibrotic drugs 5-fluoruracil and mitomycin C to trabeculectomy further improved post-operative intraocular pressure control a href="#3" title="3">3] with the result that adjunctive intraoperative treatment with drugs such as these has become popular not only in eyes at high risk for trabeculectomy failure e.g. eyes with a history of failed glaucoma surgery, aphakic eyes, African-American or Asian eyes and in eyes of patients requiring very low intraocular pressures e.g. those with normal tension glaucoma but also in eyes at relatively low risk for trabeculectomy failure undergoing primary trabeculectomy as in the case of the patient we have described here [1].

Mitomycin C inhibits Tenon’s capsule and conjunctival fibroblast proliferation [4]. It was first used in ophthalmology, topically, after pterygium excision, to prevent pterygium recurrence [5]. Its ability to modify the eye’s healing response following glaucoma surgery enables a patent filtration fistula to be established. A wide variation in the concentration e.g., 0.02 mg/ml-0.5 mg/ml, the duration of exposure e.g., 1-7 minutes and the area to which it is exposed e.g., conjunctiva +/-scleral flap for mitomycin C has been described for filtration surgery.

Histological studies have shown that mitomycin C produces a conjunctival bleb that has a thinner and more irregular epithelium overlying a largely avascular and acellular stroma [6]. Such work also noted breaks in the basement membrane of the basal layer of the conjunctiva and suggested that these areas facilitated aqueous drainage from the surface of the bleb [7]. Indeed the effects of mitomycin C on the conjunctiva following trabeculectomy are clinically evident in the appearance of the bleb created which is more commonly described as thin and avascular [6]. Aqueous humour can frequently be seen to ‘ooze’ from the conjunctiva of blebs created with mitomycin C after fluorescein application over the bleb [8].

One complication of glaucoma surgery which has been made all the more frequent by the use of adjunctive antimetabolites is postoperative low intraocular pressure [9,10]. Ocular hypotension or hypotony may be defined as an intraocular pressure less than 6 mmHg. The hyposecretion of aqueous humour and/or the excessive filtration of aqueous humour is responsible. Either can arise after trabeculectomy for several reasons which have been outlined in Table 1.

Over-filtration Loose closure of the scleral flap
Bleb leak
Hypo-secretion of aqueous humour Ciliary body shutdown accompanying ocular inflammation
Ciliochoroidal detachment
Formation of cyclodialysis cleft.
Mitomycin C ciliary body toxicity

Table 1: Causes of hypotony after trabeculectomy.

Ocular hypotension post-trabeculectomy is common and may not affect vision. The term ‘hypotony’ however is often used to imply that vision is reduced as a result of low intraocular pressure. Prolonged and/or profound hypotony may be responsible for a number of structural ocular changes which are shown in Table 2. Many of these are irreversible should they occur and so have the potential to permanently, negatively, impact on vision.

Complications of Hypotony Corneal oedema secondary to corneal de-compensation
Ciliochoroidal effusion producing choroidal detachment
Hypotonousmaculopathy i.e. a low intraocular pressure associated with changes at the fundus inclusive of cystoid macular oedema, chorioretinal folds, vascular tortuosity and/or optic nerve head oedema.
Neuronal atrophy
Phthisis bulbi

Table 2: Complications of hypotony.

Occasionally as is depicted in figure 4 a vicious cycle of hypotony, blood–aqueous barrier breakdown, ocular inflammation and ciliary body shut-down which causes a further reduction in aqueous humour production and results in even more profound hypotony can develop [11]. Indeed this is what we hypothesize happened in the case we have described here.


Figure 4: Hypotony post-trabeculectomy. A vicious circle.

The influence of post-surgical inflammation on the development of hypotony has not been widely explored. Eyes with a past history of uveitis or previous intraocular surgery as here can develop hypotony secondary to the growth of a cyclitic membrane over the ciliary body. Both iridectomy which causes a break-down of the blood aqueous barrier and poor irrigation of the anterior chamber during trabeculectomy can precipitate postoperative ocular inflammation. Further, the management of hypotony secondary to ciliary body dysfunction due to prolonged and/or severe ocular inflammation is particularly challenging, because the damaged ciliary body cannot be stimulated either medically or surgically [11].

Observation may be all that is required in the vast majority of cases of ocular hypotension/hypotony as many are often self-limiting. In one study 70% of cases of hypotony post trabeculectomy were of less than two weeks duration [12]. The treatment of hypotony becomes more urgent if it is profound, if hypotonous maculopathy which, as already mentioned, may be irreversible, develops, if the anterior chamber flattens resulting in lens-corneal touch which can promote cataract progression or if there is poor vision from the fellow eye as in the case we describe here.

Options for the management of hypotony following trabeculectomy are outlined in Table 3. Ideally treatment will eliminate hypotony while preserving filtration function and maintaining, over the long-term, a desirable intraocular pressure [13]. Initial ‘conservative’ treatment is with topical atropine to prevent pupil block and aqueous suppressants such as topical ß-blockers or oral Acetazolamide which promote healing of the surgically created fistula by temporarily reducing aqueous flow through it [13].

Non-surgical Pressure patch
Large diameter contact lens
Aqueous suppressants e.g. acetazolamide, topical ß-blockers
Cyanoacrylate glue
Injection of autologous blood around or beneath the bleb
Injection of autologous platelets beneath the bleb
Nd:YAG or Argon laser
Trichloroacetic acid abrasion
Surgical Reformation of the anterior chamber with air, viscoelastic, SF6 or C3F8 either alone or in combination
Reformation of the posterior camber with heavy liquid
Compression sutures
Scleral flap re-suture
Bleb excision and re-creation with Tenon’s capsule and/or conjunctiva
Bleb repair with sclera, pericardium, amniotic membrane, autologous conjunctival graft, conjunctival flap, cyanoacrylate glue

Table 3: The Treatment of hypotony after trabeculectomy.

If the above measures prove ineffective temporary tamponade of the conjunctiva by a pressure patch [14] or a large diameter soft bandage contact lens [15] can similarly encourage healing in the area of the fistula. The use of such non-surgical methods is relatively easy and significant complications are rare, but these methods may fail. Additionally, many of the studies describing their use pre-dates the regular use of anti-fibrotic agents and so we are left unsure of their success in treating hypotony following trabeculectomy augmented with agents such as mitomycin C.

In the case we describe here imminent lens-corneal touch necessitated re-formation of the anterior chamber. This can be accomplished with air16 but air often escapes from the anterior chamber into the bleb. Additionally, the placement of air in the anterior chamber can cause endothelial cell loss [17]. We used an ophthalmic viscoelastic device; Healon (Abbott Medical Optics, Illinois United States of America) but as it mixed well with the aqueous fluid it was rapidly cleared from the eye in the excessive aqueous outflow and its effect on the form of the anterior chamber only temporary as a result.

The injection of longer-standing gases such as those used most commonly in retinal surgery for the tamponade of retinal breaks; sulphur hexafluoride (SF6) 18 and perfluoropropane (C3F8) [19], for reformation of the anterior chamber following glaucoma surgery has also been described. These gases are not water soluble and expand at high concentrations in an aqueous medium [20] like that of the anterior chamber to form a single bubble that is helpful in both reforming the anterior chamber and sealing the fistula. The main sideeffect of the use of these gases is the development of lens opacities at the area of gas-lens contact [21].

Compression sutures may be placed through the conjunctiva +/−the scleral flap [22]. Compression sutures that are passed through the conjunctiva alone only affect the area of conjunctiva compressed and so may be insufficient in cases such as ours where there is diffuse conjunctival chemosis.

The injection of autologous blood beneath or around an overfiltering bleb may also be an acceptable treatment for hypotony that occurs secondary to excessive filtration after trabeculectomy with adjunctive anti-fibrotic therapy [23]. Blood is useful in that it can affect large areas of conjunctiva. As the goal of injecting blebs with autologous blood is to reduce bleb function the most obvious danger is the creation of a non-functioning bleb [24].

Viscoelastic may be injected into the anterior chamber before the blood to reduce the passage of blood into the anterior chamber and the creation of hyphaema [25]. It is well-known however that occlusion of the trabecular meshwork with a viscoelastic agent may acutely increase intraocular pressure. In glaucomatous eyes, such intraocular pressure spikes can cause a deterioration in visual acuity [26]. Indeed, with or without the prior injection of viscoelastic, patients, particularly those with already advanced visual field losses, need close monitoring after autologous blood injection because viscoelastic in the anterior chamber angle and/or blood flowing under the scleral flap may cause elevations in their intraocular pressure. Intra-bleb autologous platelet injection has also been used effectively for the treatment of bleb overfiltration in at least one patient [27].

Surgical intervention, for which a variety of options exist as can be seen from Table 4, many involving revision of the bleb and/or scleral flap, may eventually be required. The ideal time for such intervention and the ideal surgical technique however remains the subject of ongoing debate.

Confusion exists too regarding the association of postoperative hypotony with later bleb failure. In several series, early i.e. within one month, post-trabeculectomy hypotony was associated with reduced bleb survival time over five years [28,29]. There may be several reasons for this. Tamponade of over-filtering blebs can produce a flat bleb which may promote adhesion formation between the roof and the base of the bleb, thus detrimentally affecting bleb evolution and increasing the risk of later bleb scarring and failure. Hypotony in the early postoperative period can result in an increased breakdown of the blood aqueous barrier as already described. This can in turn, provide for the release of inflammatory mediators into the aqueous and through the filtering bleb into the surrounding tissues. Excessive postoperative inflammation can accelerate wound healing, causing scarring with eventual trabeculectomy failure [30]. When visual acuity has been restored to eyes with hypotonous maculopathy, it has often been achieved by increasing the intraocular pressure to higher than normal levels [31] which defeats the purpose of the initial trabeculectomy and so is of itself undesirable. Finally, bleb-related infection which is associated with eventual filtration failure may occur with greater frequency in dysfunctional blebs.

The management of the case we describe here was challenging. Many cases of hypotony resolve without intervention. In one review of 699 trabeculectomies performed with mitomycin C only nine cases of hypotony in which bleb revision was required were identified. However of those cases of post-surgical hypotony that develop hypotonous maculopathy close to 50% permanently lose vision [12]. The choice of observation or intervention when the better seeing eye of a patient is affected by hypotony is a difficult one to make. Taking the route of conservative treatment in the case we describe here meant our patient spent a very protracted period of time in hospital. Reducing this patient’s topical steroid treatment as suggested, we hoped, would allow for healing of the over-filtering fistula but may well have provided for an increase in intraocular inflammation which could, via its effects on the ciliary body, exacerbate hypotony.


Prolonged and intractable post-surgical hypotony is rare but the loss of vision which may accompany it is devastating for the patient particularly when he/she may have poor vision from their fellow eye. Further, it may be that even if hypotony resolves over the short term, there persists an increased risk for filtration failure over the longer term. As with much in ophthalmology, prevention is often better than cure and so anti-fibrotic agents should be used as adjuncts in glaucoma surgery judiciously and due attention given to the meticulous closure of both the scleral flap and conjunctiva during trabeculectomy.

Reasons for Case Selection

• With the ongoing increase in the life expectancy of patients and an anticipated increase in the size of our aged population we will be increasingly made to consider the appropriateness of trabeculectomy with or without adjunctive anti-metabolites in relatively older patients as in the case described here.

• Hypotony is the most common complication of trabeculectomy with mitomycin C. Its significance is however often overlooked. Herein we hope to emphasize the relatively frequent occurrence of hypotony after trabeculectomy; up to 72% of trabeculectomies in one series [12], and highlight its possible adverse effects on longerterm surgical outcome.

• While hypotony is common that which requires surgical intervention is uncommon. We wish to describe some of the ‘lesser known’ ways in which hypotony can be treated.

• This case indicates that there is much room for improvement in our ability to provide timely, appropriate, convenient follow-up care for our glaucoma patients.

Lessons Learned

The post-trabeculectomy complication described here may have been prevented by the more judicious use of mitomycin C in this patient e.g., the application of mitomycin C could have been omitted completely, a lower concentration of the drug could have been utilized, or the duration of exposure of the conjunctiva to this agent reduced.

The social circumstances of the lady we describe in this report necessitated her prolonged in-patient care while she regained her vision postoperatively. In considering the most appropriate care plan for individual glaucoma patients such circumstances should undoubtedly be given more weight.

Patience is a virtue. Management need not always be active. Watchful waiting is often most appropriate.


Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Recommended Conferences

Article Usage

  • Total views: 8377
  • [From(publication date):
    October-2016 - Mar 25, 2018]
  • Breakdown by view type
  • HTML page views : 8284
  • PDF downloads : 93

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version