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Immunopathological Diagnosis of Mucous Membrane Pemphigoid (MMP) | OMICS International
ISSN: 2165-7920
Journal of Clinical Case Reports
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Immunopathological Diagnosis of Mucous Membrane Pemphigoid (MMP)

Pejcic A1, Zivkovic V2, Pesic Z3, Obradovic R1, Awan K4, Dencic T2, Minic I1*, Mirkovic D1 and Zarev M3

1Department of Periodontology, Medical Faculty, University of Nis, Serbia

2Institute for Pathology, Medical Faculty, University of Nis, Serbia

3Department for Maxillo Facial Surgery, Medical faculty, University of Nis, Serbia

4Department of Oral Medicine and Diagnostic Sciences, College of Dentistry, King Saud University, Riyadh, Saudi Arabia

Corresponding Author:
Ivan Minic
Department of Periodontology
Medical Faculty, University of Nis, Serbia
Tel: +381643004883
E-mail: [email protected]

Received date: May 20, 2016; Accepted date: June 22, 2016; Published date: June 27, 2016

Citation: Pejcic A, Zivkovic V, Pesic Z, Obradovic R, Awan K, et al. (2016) Immunopathological Diagnosis of Mucous Membrane Pemphigoid (MMP). J Clin Case Rep 6:825. doi:10.4172/2165-7920.1000825

Copyright: © 2016, Pejcic A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Background: Mucous membrane pemphigoid (MMP) is a serious, autoimmune, blistering disorder which primarily involves the mucous membranes and that can result in blindness and other complications as a result of scarring of mucous membranes. The oral cavity and the eye are most frequently involved. Since extension of the lesion into pharynx causes dysphagia and progressive ocular lesions may cause blindness, early and valid diagnosis is very important. Diagnosis of MMP is a problem because patients afflicted are elderly, the disease is chronic, and is often in an advanced stage when the diagnosis is established and requires aggressive therapy for control. Oral and dental care is important in all phases of the diagnosis and treatment of the patient with mucous membrane pemphigoid. Early recognition and diagnosis are important for early treatment, improving survival and for limiting the complications of therapy. Methods: Here we present a case of mucous membrane pemphigoid with onset at age 49 in patient who manifested severe periodontal disease and showed the lesion only on the mucous membrane of the gingiva. We describe how we made a differential diagnosis from other diseases which also accompany similar lesions. Results: We examined the clinical manifestations, blood test results, histopathologic findings of the affected tissue, and immunological findings in relation to autoimmunity. Conclusion: We believe that the diagnostic strategy described in the present study will be quite informative for making rapid and definite diagnoses of similar cases.

Keywords

Mucous membrane pemphigoid; Diagnosis; Therapy; Immunopathology; Histopathology

Introduction

Mucous membrane pemphigoid (MMP) is a heterogeneous group of blistering autoimmune bullous disorder of the skin and mucosae and unknown etiology [1,2]. Intraoral manifestations of MMP feature the formation of vesiculobullous lesions that eventually rupture, leading to pseudo membrane covered irregular-shaped ulcerations. The presence of these often painful oral lesions may hinder oral hygiene efforts resulting in increased plaque accumulation and may increase the risk of developing periodontal disease [3]. The success of any treatment depends on a proper and correct diagnosis. However, MMP frequently presents diagnostic problems because the clinical symptoms often resemble other diseases showing desquamated gingivitis and histological examination of biopsies cannot always differentiate MMP from these diseases [4]. Since the oral membrane is most frequently involved, periodontitis have an opportunity to meet MMP patients before a definite diagnosis is made. Since oral lesions may progress if untreated, a definite diagnosis is an important component in the overall program of therapy for MMP patients [5]. We aimed to develop consensus–based recommendations for medical communication among various health care professional, to improve accuracy of diagnosis and treatment and to facilitate future investigations for mucous membrane pemphigoid.

Case Report

A 49-year-old Serbian woman was in good health but with spontaneous bleeding from gingiva and painful desquamation of the gingiva. A year and a half before she visited her dentist but her problem was not resolved. After that, a gingival biopsy was taken, however, the histology showed only non-specific gingivitis. She received oral cavity irrigation and topical application of corticosteroid for about 3 months. Clinical symptoms did not improve and she was referred to us, to Dental Clinic in Nis at the Department of Periodontology and oral medicine for further examination and treatment. Clinical oral examination revealed redness, epithelial desquamation, swelling and vesiculation of the gingiva. The diagnosis of periodontal disease was made based on the medical history, clinical findings and results of the radiographic examination (Figure 1).

clinical-case-reports-Clinical-condition-redness

Figure 1: Clinical condition with redness, desquamation and swelling of the gingiva.

The results of laboratory examinations were within normal ranges and data not shown.

The biopsy of the gingival tissue was taken in the Department for Maxillofacial surgery at Dental Clinic in Nis and sent to the Institute of Pathology, Medical faculty in Nis. Histopathological examination revealed separation of the epithelium from the basement membrane, but not within the epithelium and sub epidermal bulla with fibrin net and inflammatory infiltrate. Massive infiltrates were observed in the connective tissues (lymphocytes, polymorphonuclear leukocytes and plasma cells) (Figures 2 and 3).

clinical-case-reports-epithelium-basement

Figure 2: Separation of the epithelium from the basement membrane, but not within the epithelium (HEx200).

clinical-case-reports-Bullous-pemphigoid–sub

Figure 3: Bullous pemphigoid–sub epidermal bula with fibrin net and inflammatory infiltrate (Hex200).

Immunological examination for differential diagnosis of autoimmune disease was performed at the Central laboratory for immunological examination at Medical faculty in Nis. By indirect immunofluorescence, the patient’s IgG reacted with the basement membrane zone of human gingiva sections (Figure 4).

clinical-case-reports-Presence-IgG-deposits

Figure 4: Presence of IgG deposits (indirect immunofluorescence, original magnification x400).

Based on the clinical findings and the results of immunological examination, diagnosis of this patient was MMP with advanced periodontitis. Treatment of MMP consisted of systemic and topical administration of corticosteroid (Dexamethasone at a dosage of 1.5 mg daily and tapered to 0.5 mg daily). In addition to steroid therapy, periodontal treatment was performed with conventional conservative therapy. Several months later, following therapy, the gingival appearance improved dramatically.

Discussion

Mucous membrane pemphigoid is a subepithelial vesiculobullous disorder mainly of late middle age; it has a slight predilection for women [6]. Whereas mucous membrane pemphigoid was formerly considered a single entity, it is now quite evident that a number of subepithelial vesiculobullous disorders may produce similar clinical pictures and also that a range of variants of mucous membrane pemphigoid exists, with antibodies directed against various hemidesmosomal components or components of the epithelial basement membrane. Mucous membrane pemphigoid, a chronic systemic autoimmune blistering disease that affects any mucous membrane, is characterized by linear deposition of IgG, IgM, IgA or C3 along the epithelial basement membrane that leads in most mucosal tissue to progressive scar formation [7,8].

Diagnosis and management of immune-mediated sub-epithelial blistering diseases on clinical grounds alone are impossible; a full history, general and oral examinations, and biopsy with immune staining are now invariably required, sometimes supplemented with other investigations. Most patients with mucous membrane pemphigoid affecting the mouth manifest desquamative gingivitis, a fairly common complaint typically seen in women who are middleaged or older [4,9]. Oral vesicles and erosions may also occur, and there can be a positive Nikolsky sign. Some patients have lesions of other stratified squamous epithelia, presenting as conjunctival, nasal, esophageal, and laryngeal, vulval, penile, or anal involvement. Apart from improving oral hygiene, immunomodulatory-in particular, immunosuppressive-therapy is typically required to control oral lesions in mucous membrane pemphigoid. No single treatment regimen reliably controls all these disorders.

The present case exhibited desquamative gingivitis and severe periodontal disease. The differential diagnosis of MMP includes Pemphigus vulgaris, Stevens-Johnson syndrome, Erythema multiform, Paraneoplastic pemphigus and Pseudopemphigoid,as and oral viral infection [3,10,11]. Based on the clinical findings and patient`s history, pemphigus vulgaris, paraneoplastic pemphigus and pseudopemphigoid were suspected. Differential diagnosis among these diseases is especially important. In MMP, oral mucosal lesions frequently extend into the pharynx and esophagus causing a sore throat and dysphagia, and severe ocular involvement nay cause blindness.

Histopathological examination using tissue sections of gingiva revealed separation of the epithelium from the basement membrane zone. These findings suggested the possibility of pemphigoid, but not pemphigus. Early in disease, infiltration of T lymphocytes, plasma cells, polymorphonuclear leukocytes and occasional eosinophils are present. In the acute phase, there are numerous polymorphonuclear leukocytes, and in the later phases, sub-epithelial fibrosis ensues. In order to make a definite diagnosis, indirect immunofluorescence was performed. The patient`s IgG reacted with the basement membrane zone of human gingiva sections [12]. Medical treatment is a multidisciplinary approach that is essential in the management of MMP. The choice of agents for treatment of MMP is based upon the sites of involvement, clinical severity, and disease progression.

Because MMP is a systemic disease, topical therapy alone is insufficient. Evaluation of the clinical picture-combined with the severity of inflammation, location and rapidity of progression-should direct selection of anti-inflammatory medical treatment. For patients with severe, rapidly progressive disease, the treatment should consist of prednisone (1 mg to 1.5 mg per kg body weight per day) and cyclophosphamide or azathioprine [13]. The most common side effects of cyclophosphamide include myelosuppression and hemorrhagic cystitis, whereas azathioprine most commonly causes gastrointestinal discomfort, although reversible hepatic toxicity can occur in 2% of patients. Patients need to be carefully monitored by their internist for these complications. For less progressive disease, dapsone (50 mg to 200 mg per day) can be considered as an alternative initial therapy [14]. Dapsone treatment should be avoided in sulfa-allergic patients. Other promising treatments include mycophenolate mofetil combined with dapsone and steroid, intravenous immunoglobulin, and biologics such as rituximab and daclizumab. Immunosuppressive treatment is usually continued 2 to 3 years into remission [15,16].

Topical treatments are often used as adjunctive therapy in the treatment of MMP, and occasionally as monotherapy in patients with limited oral disease [17]. Successful treatment of oral lesions has been reported with the use of topical steroids, tacrolimus, and cyclosporine [18,19]. Topical therapy of MMP involving the oral mucosa can be augmented with specialized application devices and administration at bedtime when oral secretions are relatively low. The patients diagnosed with MMP appear to be no more at risk an increased progression periodontal disease. Oral care includes appropriate dental hygiene, referral for dental care, monitoring for candidosis, and topical anesthetics for pain. Soft, bland foods may cause less injury to eroded mucosa when disease is flaring. Fortunately, oral disease does not commonly result in functionally significant scarring.

Conclusions

In this case, based on the clinical findings and the results of immunological examination, we diagnosed that this patient has MMP accompanied with advanced periodontitis. The initial oral findings and diagnosis and treatment in this case of mucous membrane pemphigoid are presented. Histologic and immunologic studies were undertaken in this case to confirm the clinical diagnosis. Early diagnosis and aggressive local and systemic therapy are critical to long-term visual outcome in cases of mucous membrane pemphigoid. The treatments prescribed illustrate that topical steroids are effective, but in this case systemic steroid therapy with or without other immunological active drugs is required. The diagnosis of MMP remains a challenge as patients may present to a variety of specialists, depending upon their initial symptomatology, and due to the diverse clinical manifestations.

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