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In situ localization of Chlamydia trachomatis and Chlamydial Heat Shock Protein 60 in Endometrial Curettage Tissue of Recurrent Spontaneous Aborters | OMICS International
ISSN: 2157-7099
Journal of Cytology & Histology

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In situ localization of Chlamydia trachomatis and Chlamydial Heat Shock Protein 60 in Endometrial Curettage Tissue of Recurrent Spontaneous Aborters

Sangita Rastogi1*, Chanchal Yadav1 and Banashree Das2

1Microbiology Laboratory, National Institute of Pathology (ICMR), Sriramachari Bhawan, Safdarjung Hospital Campus, Post Box No. 4909, New Delhi-110029, India

2Department of Obstetrics and Gynecology, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi-110029, India

*Corresponding Author:
Dr. Sangita Rastogi
Microbiology Laboratory
National Institute of Pathology (ICMR)
Sriramachari Bhawan, Safdarjung Hospital Campus
Post Box No. 4909, New Delhi-110029, India
Tel: +91-09810217260
E-mail: [email protected]

Received Date: August 24, 2015 Accepted Date: September 08, 2015 Published Date: September 10, 2015

Citation: Rastogi S,Yadav C, Das B (2015) In situ localization of Chlamydia trachomatis and Chlamydial Heat Shock Protein 60 in Endometrial Curettage Tissue of Recurrent Spontaneous Aborters. J Cytol Histol 6:373. doi:10.4172/2157-7099.1000373

Copyright: © 2015 Rastogi S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Description

In India, genital infection with Chlamydia trachomatis is a major health problem in women because of high prevalence (upto 81%). During pregnancy, chlamydial infection may cause various perinatal complications including spontaneous abortion [1,2].

Also, immunity to chlamydial heat shock protein 60 (chsp60) is associated with upper genital-tract infection and presence of chsp60 may regulate process of abortion by stimulating macrophage function [2]. Hence, aim of this study was to investigate whether C. trachomatis is associated with spontaneous abortion in patients with prior history of three or more spontaneous abortions, viz.: Recurrent Aborters (RA). We used immunohistochemical approach to localize chlamydial infection within the endometrial curettage tissue (ECT) in women with spontaneous abortion. Immunohistochemical analysis confirmed C. trachomatis in ECT (11/ 100) (Figure 1). Also, double staining was done for In situ localization of macrophages (CD 68) and chsp60 (within the macrophages) in ECT (Figure 2). chsp60 was co-localized within macrophages in (72.7%) infected RA. Serum level of IgG antibodies to chlamydial heat shock protein-60 (chsp60) was determined by using commercial kit as per manufacturer's recommendations (Medac, Germany). Among the Chlamydia-positive RA, 63.6% (7 of 11) showed presence of serum chsp60 antibodies. Results suggest definite need for C. trachomatis screening in women experiencing spontaneous abortion to prevent recurrent miscarriage.

cytology-histology-in-situ-localization

Figure 1: In situ localization of elementary body of Chlamydia trachomatis in the endometrial curettage tissue from patient undergoing recurrent spontaneous abortion (400X).

cytology-histology-dual-staining

Figure 2: Dual staining for chlamydial heat shock protein 60 (red colour) and macrophages (CD 68; brown colour) in the endometrial curettage tissue from Chlamydia trachomatis -infected patient experiencing recurrent spontaneous abortion (200X).

Acknowledgements

This work was supported by research project grant from Life Sciences Research Board, Defence Research & Development Organization, Government of India, New Delhi, India.

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