Received Date: August 24, 2015 Accepted Date: September 08, 2015 Published Date: September 10, 2015
Citation: Rastogi S,Yadav C, Das B (2015) In situ localization of Chlamydia trachomatis and Chlamydial Heat Shock Protein 60 in Endometrial Curettage Tissue of Recurrent Spontaneous Aborters. J Cytol Histol 6:373. doi:10.4172/2157-7099.1000373
Copyright: © 2015 Rastogi S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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In India, genital infection with Chlamydia trachomatis is a major health problem in women because of high prevalence (upto 81%). During pregnancy, chlamydial infection may cause various perinatal complications including spontaneous abortion [1,2].
Also, immunity to chlamydial heat shock protein 60 (chsp60) is associated with upper genital-tract infection and presence of chsp60 may regulate process of abortion by stimulating macrophage function . Hence, aim of this study was to investigate whether C. trachomatis is associated with spontaneous abortion in patients with prior history of three or more spontaneous abortions, viz.: Recurrent Aborters (RA). We used immunohistochemical approach to localize chlamydial infection within the endometrial curettage tissue (ECT) in women with spontaneous abortion. Immunohistochemical analysis confirmed C. trachomatis in ECT (11/ 100) (Figure 1). Also, double staining was done for In situ localization of macrophages (CD 68) and chsp60 (within the macrophages) in ECT (Figure 2). chsp60 was co-localized within macrophages in (72.7%) infected RA. Serum level of IgG antibodies to chlamydial heat shock protein-60 (chsp60) was determined by using commercial kit as per manufacturer's recommendations (Medac, Germany). Among the Chlamydia-positive RA, 63.6% (7 of 11) showed presence of serum chsp60 antibodies. Results suggest definite need for C. trachomatis screening in women experiencing spontaneous abortion to prevent recurrent miscarriage.
This work was supported by research project grant from Life Sciences Research Board, Defence Research & Development Organization, Government of India, New Delhi, India.
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