Reach Us +44-1625-708989
Low Sucrose and High Gibberellic Acid Levels Positively Regulates Relative Growth Rate and Organogenesis in D. Membranaceus Munro | OMICS International
ISSN: 2161-1009
Biochemistry & Analytical Biochemistry

Like us on:

Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Low Sucrose and High Gibberellic Acid Levels Positively Regulates Relative Growth Rate and Organogenesis in D. Membranaceus Munro

Jasmine Brar*1, Manju Anand1 and Anil Sood2

1Department of Biotechnology, Thapar University, Patiala, Punjab, India

2Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, Palampur, HP, India.

*Corresponding Author:
Jasmine Brar
Department of Biotechnology
Thapar University Patiala
Punjab-147004, India
E-mail: [email protected]

Received Date: June 24, 2015; Accepted Date: July 07, 2015; Published Date: July 09, 2015

Citation: Brar J, Anand M, Sood A (2015) Low Sucrose and High Gibberellic Acid Levels Positively Regulates Relative Growth Rate and Organogenesis in D. Membranaceus Munro. Biochem Anal Biochem 4:198. doi:10.4172/2161-1009.1000198

Copyright: © 2015 Brar J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Biochemistry & Analytical Biochemistry



Dendrocalamus membranaceus Munro., an economically important edible bamboo has been listed as a priority species requiring conservation by International Union for Conservation of Nature (IUCN) and hence selected for the present investigations. It is opined that the cells of bamboo are undoubtedly totipotent but some vital hormonal and/or nutritional factor or their combination could prove vital for differentiation as the frequency of embryogenesis is lower than that of organogenesis [1]. The role of organic compounds like sucrose and growth regulators like Gibberellic acid (GA3) effects the relative growth rate (RGR) of callus in–turn effecting overall organogenesis and ultimately leading towards somatic embryogenesis. Carbohydrates are transported within the plants mainly in the form of sucrose and the level of sugar within the medium needs to be optimum for proper organogenesis [2]. GA3 was used for enhanced growth and to promote cell elongation during shoot bud differentiation in D. hamiltonii [3,4]. Takeno et al., [5] demonstrated that the relatively high levels of endogenous GA-like substances were required at early stages of embryogenesis followed by a rapid decrease of free GAs. Till date the roles of auxins and cytokinins in organogenesis/embryogenesis were investigated very well, however, we do not have a complete understanding of the involvement of GA3 in organogenesis as its effects vary in different tissues and species and needs further more investigations.

Role of Sucrose

Researchers have claimed that the addition of high concentration of sucrose in the culture media might have inhibitory effect on nutrient uptake by lowering water potential of the medium [6]. Growth rate studies were carried out using various concentrations of sucrose ranging from 1 to 5% as shown in Figure 1a-e and Table 1. Sucrose (1%) with a RGR of 0.46 ± 0.0 mg/g/day with a corresponding increase in Growth Index (5.62 ± 0.50) and Cell Increase Ratio (4.62 ± 0.50) was observed. RGR kept on decreasing as the concentration of sucrose was increased to 5% keeping rest of the medium composition similar. Thus a definite correlation between sucrose concentration and RGR was established confirming the role of sucrose as the main carbon source for providing energy and increasing the biomass. It has also been indicated that sucrose concentration influences the overall growth of callus, browning and its proliferation [7]. In Dendrocalamus asper, Arya et al., [8] found 2% sucrose to be best for callus growth while higher sucrose (6%) concentration retarded the callus growth and its multiplication. Similarly, in B. edulis, Lin et al., [9] used 3-6% sucrose and found that 3% sucrose gave the best response for embryogenic callus proliferation. In contrast, Yeh and Chang [10] found 6% sucrose to be beneficial for callus proliferation in B. beecheyana. In D. membranaceus, best results regarding callus proliferation were obtained using 1% sucrose as also reported by Rao et al., [11] in D. strictus.


Figure 1: Relative growth of callus on different concentrations of sucrose showing highest.

Sucrose % RGR mg/g/day GI CIR Colour of Callus
1 0.46a ± 0.05 5.62a ± 0.50 4.62a ± 0.50 Creamish
2 0.32b ± 0.02 4.25b ± 0.24 3.25b ± 0.24 Creamish+light brown
3 0.24c ± 0.02 3.40c ± 0.23 2.40c ± 0.23 Creamish+light brown
4 0.16d ± 0.02 2.66d ± 0.23 1.66d ± 0.23 Creamish
5 0.11e ± 0.01 2.11e ± 0.16 1.11e ± 0.16 Creamish

Table 1: Effect of sucrose concentrations on relative growth rate (RGR).

Gibberellic acid (GA3)

As the application of GA3 was found to have an effect on the growth rate in many bamboo species, experiments were conducted using varying concentrations of GA3 (1.1 to 2.8 μM) in the media (Figures 2a-2c) alongwith 4.53 μM of 2,4-D. RGR was found to be maximum i.e. 0.63 ± 0.05 mg/g/day when 2.8 μM of GA3 was used (Table 2) with a GI of 7.37 ± 0.50 and a CIR of 6.37 ± 0.50. Our results were in consonance with Gautam et al., [12] where also high concentrations of GA3 (> 5 μM; 1-8 mg/l) positively regulated the growth of undifferentiated callus cells in Matthiola incana.


Figure 2: Growth of callus on a. 1.1 μM of GA3 b. 1.7 μM of GA3 c. 2.8 μM of GA3.

Concentration of GA3 in µM RGR mg/g/day GI CIR
1.1 0.24c ± 0.02 3.46c ± 0.29 2.46c ± 0.29
1.7 0.41b ± 0.03 5.18b ± 0.31 4.18b ± 0.31
2.8 0.63a ± 0.05 7.37a ± 0.50 6.37a ± 0.50

Table 2: GA3 and Relative Growth Rate (RGR).

This is the firstreport in D. membranaceus citing that the optimum concentration of organic supplements and GA3enhances the rates of callus growth and organogenesis. Further, more research needs to be carried out to improve the frequency of plant regeneration and reveal the factorsaffecting plant regeneration)


The authors are thankful to Dr. P.S. Ahuja, Ex-Director, CSIR-IHBT for providing laboratory facilities for carrying out this research work.


Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Relevant Topics

Article Usage

  • Total views: 12643
  • [From(publication date):
    October-2015 - Jan 24, 2019]
  • Breakdown by view type
  • HTML page views : 8838
  • PDF downloads : 3805

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2019-20
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri and Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

ankara escort

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

pendik escort

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2019 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7