alexa New Bioplastic Material Based on Hyaluronic Acid Hydrocolloid | OMICS International
ISSN: 2155-9554
Journal of Clinical & Experimental Dermatology Research

Like us on:

Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

New Bioplastic Material Based on Hyaluronic Acid Hydrocolloid

Zinoviev EV1, Rakhmatullin RR2 and Almazov IA3*

1Researcher, Research Laboratory (war surgery), Research Centre, Military Medical Academy Named After S.M. Kirov, RF Defence Ministry, St. Petersburg, Russia

2Head, Scientific and Production Laboratory of Cell Technologies, Orenburg State University, Russia

3Clinical Resident, VII Department, Military Medical Academy named after S.M. Kirov, RF Defence Ministry, St. Petersburg, Russia

*Corresponding Author:
Almazov Ilya Alekseevich
Clinical Resident, VII Department
Military Medical Academy named after S.M. Kirov
RF Defence Ministry, St. Petersburg
37a Acad. Lebedev Str., Russia
Tel: +79119244709
E-mail: [email protected]

Received date: January 31, 2014; Accepted date: March 17, 2014; Published date: March 24, 2014

Citation: Zinoviev EV, Rakhmatullin RR, Almazov IA (2014) New Bioplastic Material Based on Hyaluronic Acid Hydrocolloid. J Clin Exp Dermatol Res 5:215. doi: 10.4172/2155-9554.1000215

Copyright: © 2014 Zinoviev EV, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Journal of Clinical & Experimental Dermatology Research

Abstract

Relief of the typical pathological processes and the early activation of regenerative mechanisms are the conditions for the successful wound healing. Reparative potential of epithelial tissues is realized at a balanced migration and proliferation of their cellular elements, synthesis of fibrous proteins and extracellular matrix components, epithelisation. An in-depth assessment was made of the structure of the developed bioplastic material based on hyaluronic acid hydrocolloid (G-Derm) and of its properties, positive in terms of optimizing the reparative mechanisms. Scanning probe microscopy and ultrastructure evaluation of bioplastic material were performed. For investigating the visualized globular structures, homogeneous samples of the material length 101.5 ± 11.2 nm, width 110.3 ± 10.7 nm, height 23.4 ± 3.4 nm were taken. The space between globular formations was 127.2 ± 21.3 nm. It was found that the roughness coefficient of the surface relief (Rq) was 8.7 ± 0.5 nm. The method of fixing the contact angle of water was used to evaluate hydrophilic/hydrophobic properties of biomaterial. Value of the angle was 83°; adhesion coefficient was 99.88 mN/ m2, which characterizes the surface of biomaterial as moderately hydrophilic. Mesenchymal stromal stem cells were cultured on a substrate of bioplastic material based on hyaluronic acid hydrocolloid. In the course of ultrastructural study cell migration into the material was recorded, presence between the interwoven fibrillar fibres of cell layers of an oblong shape 3.7 ± 0.5 mcm wide, which reflects their cooperation with the surface of the developed polymer suitable for the use as a growth substrate in case of biotechnological replacement of tissue defects.

Keywords

Hyaluronic acid; Bioplastic material; G-Derm; Cell carrier; Biomaterial ultrastructure; Mesenchymal stromal stem cells

Introduction

Relief of typical pathological processes (microcirculatory disorders, interstitial oedema, uncontrolled inflammatory reaction, respiratory burst, acidosis, hypercytokinemia) and early activation of regenerative mechanisms are the conditions for successful healing of defects in epithelial tissues of various etiologies. Reparative potential of epithelial tissues is realized at a balanced migration and proliferation of their cellular elements, synthesis of fibrous proteins and extracellular matrix components, epithelisation [1]. For the purpose of plastic reconstruction of epithelial tissue defects as well as for ensuring self-healing of wounds and burns, various bioplastic materials based on synthetic and natural (preferably) polymers are extensively used [2,3]. Determination of the optimal physicochemical, mechanical and biological properties of natural bioplastic materials used for replacement of the epithelial tissue defects is extremely important and relevant in particular in terms of the ability of these polymers to ensure the optimal conditions for skin cells adhesion, migration and proliferation [4,5]. Study of the structural features of biopolymer material is important for biomaterials intended for plastic reconstruction of the epithelial tissue defects [2]. Publications contain information about the evaluation of relief and hydrophilicity/hydrophobicity of a number of natural polymers such as collagen, cellulose, chitosan as well as the materials obtained from resorbable polyhydroxyalkanoates (PHAs) [6]. It is shown that the relief and surface roughness of polymers in the nanoscale range can determine the adhesion, spreading and motion activity of cells and also influence the synthesis of specific extracellular matrix proteins. The presence of the surface potentials, specific features of the surface structure as well as its composition are the necessary characteristic which can affect the viability and proliferation of cells at interaction with bioplastic materials [7-9].

Purpose of Investigation

Experimental study of ultrastructure and biological properties of histoequivalent-bioplastic material based on hyaluronic acid hydrocolloid.

Materials and Methods

The object of study was histoequivalent-bioplastic material based on hyaluronic acid hydrocolloid (G-Derm) produced photochemically (RF patents No. 2425694, 2367476, 2458709, 2481127). Samples of biomaterial were studied by scanning atomic force microscopy (AFM) in contact mode (multimicroscope “SMM-2000”, Open Joint Stock Company OAO “Proton-miet”, and Russia). In the process of scanning cantilevers “MSCT-AUNM” manufactured by “Veeco Instruments Inc.” (United States of America) were used with beam stiffness 0.05 N/m, of a pyramid shape and curvature radius of the probe 10 nm. Quantitative morphometric analysis was performed using regular software for the microscope. Mechanical properties of bioplastic material were studied using AFM in power spectroscopy mode, at which the cantilever deflection is no longer dependent on the distance between the needle tip and the sample surface. For assessing the hydrophilic properties of the material as a parameter integrally characterizing its interaction with the polar solvent (water) the work of adhesion (Wa) was used determined from the evaluation results of the equilibrium limiting wetting angles [2,3]. Five mcl of distilled water (at temperature of 20-21°C.) were applied on the surface of biomaterial; 30 seconds later, photographing was accomplished, and determination of the values of limiting wetting angles ((θ) was carried out at the liquid/solid phase interfaces [2,3]. The work of adhesion was calculated from the formula:

Wα =σ •(1+cosθ) (1)

where σ is the surface tension coefficient of water at 20°C taken as 72.86×0.001 mN/m; θ, value of static limiting wetting angle. Taking into account the porosity and developed relief characterized by high roughness values, the calculation of the adhesion forces on the basis of the limiting wetting angle was carried out taking into account the roughness coefficient (the ratio of true surface area to geometric area) [10].

The results of the investigation were processed by the analysis of variance.

Results and Discussion

It has been ascertained that histoequivalent-bioplastic material based on hyaluronic acid hydrocolloid visualized by scanning AFM has a morphologically homogeneous surface, the surface ultrastructure being represented by globular formations of the same type (Figure 1).

clinical-experimental-dermatology-acid-hydrocolloid

Figure 1: Surface structure of unstructured biomaterial based on hyaluronic acid hydrocolloid. Magnif. ×16,000.

One of the most important characteristics of the images obtained by scanning AFM is 3D geometry of the objects which allows a detailed analysis of their morphology along the drawn profiles and performing morphometry (Figure 2).

clinical-experimental-dermatology-globular-structure

Figure 2: Structure of the profile of globular structure of material surface based on hyaluronic acid hydrocolloid.

In the course of morphometry it was determined that the dimensional parameters of the visualized globular structures of bioplastic material based on hyaluronic acid hydrocolloid were as follows: width 110.3 ± 10.7 nm, height 23.4 ± 3.4 nm. The space between globular formations reached 127.2 ± 21.3 nm. The analysis of the development degree of relief using the parameter of mean square roughness (Rq, deviation of profile points from its midline) showed that the surface of bioplastic material based on hyaluronic acid hydrocolloid was a uniform texture with Rq values about 8.7 ± 0.5 nm (Figure 3).

clinical-experimental-dermatology-surface-material

Figure 3: Results of evaluating mean square roughness of the surface of material based on hyaluronic acid hydrocolloid.

A possibility of adhesion and migration of somatic cells of epithelial tissues on the surface of bioplastic material based on hyaluronic acid hydrocolloid is determined, on the one hand, by its hydrophilic/ hydrophobic properties; and, on the other hand, by microrelief. It is known that the fastening of fibroblasts and keratinocytes is more likely to occur on the surface of material having high surface energy, on a hydrophilic surface. At the same time, basic cellular processes (growth, differentiation, migration) are strongly affected by geometric and dimensional characteristics of the substrate relief [11,12].

In the course of assessing hydrophilic/hydrophobic properties of biomaterial it was established that the value of the contact angle of water was 83°, and Wa force was 99.88 MN/m2, which characterizes the surface of histoequivalent-bioplastic material as moderately wettable [12,13].

Additional visualization of biopolymer surface in registration mode of Wa forces allowed localizing nanoregions with elevated adhesion (Figure 4).

clinical-experimental-dermatology-forces-surface

Figure 4: Distribution structure of Wa forces on the surface of material based on hyaluronic acid hydrocolloid. Scale-500 nm.

When considering the obtained data on physical properties of the developed bioplastic material based on hyaluronic acid hydrocolloid and linking them with a possibility of its use as a substrate when culturing somatic cells it was revealed that the morphological structure of biomaterial was mainly represented by globular formations forming a branched 3D structure with pore complexes from tens to hundreds of nanometres in diameter. Such 3D configuration determines a possibility of free diffusion of tissue fluid through the entire volume of the material. An important feature of this material is the stability of its morphological and mechanical parameters in response to wetting, which appears to be a favourable condition for using the material in the wound healing process.

Evaluating thermodynamic action of water on the surface of histoequivalent bioplastic material based on hyaluronic acid hydrocolloid from the limiting contact angle it was found that the recorded values of adhesion forces characterize it as moderately hydrophilic material. Such a result can be explained by the presence of air in cavities and pores of biomaterial, which prevents initial penetration of water. Thus, hydrophilic/hydrophobic properties of biopolymer appear to be dependent not only on physicochemical properties, but also on its structural features. This was confirmed by direct visualization of biomaterial surface in the adhesion-contact AFM mode. The obtained results point to occurrence on the surface of biomaterial of areas with adhesive properties which are rather large with respect to the visualized area.

When evaluating the cultivation of mesenchymal stromal stem cells on the substrate of histoequivalent-bioplastic material based on hyaluronic acid hydrocolloid by AFM method it was found that the cells of an oblong shape and 3.7 mm wide are detected on its surface (Figure 5a). In-depth study revealed the presence on the cell surface of intertwined fibres of, presumably, secondary and tertiary structure of collagen (Figure 5b).

clinical-experimental-dermatology-surface-biomaterial

Figure 5: Mesenchymal stem cell on the surface of biomaterial: a, 5 mcm scale; b, 2 mcm scale Arrangement of mesenchymal stem cells on the surface of biomaterial points to their migration processes, synthesis of fibrous proteins, active interaction with the substrate surface as well as penetration of hyaluronic acid hydrocolloid into deep layers.

Conclusion

1. Histoequivalent-bioplastic material based on hyaluronic acid polymer (G-Derm) maintains the stability of morphological and mechanical parameters in response to wetting, which appears to be favourable for creating the optimal conditions of humid environment during adhesion and migration of mesenchymal stem cells.

2. Material based on hyaluronic acid hydrocolloid is moderately hydrophilic and the hydrophilic/ hydrophobic properties of biopolymer appear to be dependent on physicochemical and structural properties.

3. After culturing of mesenchymal stromal cells, their presence is recorded in the structure of biomaterial as well as migration and protein-synthetic activity both on the surface, and in the structure of the polymer.

References

Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Relevant Topics

Recommended Conferences

Article Usage

  • Total views: 12369
  • [From(publication date):
    March-2014 - Jun 22, 2018]
  • Breakdown by view type
  • HTML page views : 8546
  • PDF downloads : 3823
 

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7