alexa Next Generation Sequencing Technologies: A Short Review | Open Access Journals
ISSN 2469-9853
Journal of Next Generation Sequencing & Applications
Like us on:
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Next Generation Sequencing Technologies: A Short Review

Tarek Hamed Attia1* and Maysaa Abdallah Saeed2
1Pediatric Department, Faculty of Medicine, Zagazig University, Egypt
2Tropical Medicine Department, Faculty of Medicine, Zagazig University, Egypt
Corresponding Author : Tarek Hamed Attia
Professor of Pediatrics, Zagazig University
Zagazig, Zagazig University
Faculty of Medicine, Pediatric Department, Egypt
Tel: 00201276099800
E-mail: [email protected]
Received: January 25, 2016 Accepted: February 9, 2016 Published: February 12, 2016
Citation: Attia TH, Saeed MA (2016) Next Generation Sequencing Technologies: A Short Review. Next Generat Sequenc & Applic S1:006. doi:10.4172/2469-9853.S1-006
Copyright: © 2016 Attia TH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Journal of Next Generation Sequencing & Applications

Abstract

When the editorial board invited me to write this short review, I remembered my old dream since thirty years ago, is it possible for mankind to live without more health burden? Scientists can answer a lot of difficult questions like this one. The science is growing very fast and next generation sequencing technologies are currently the hottest topic in the field of human and animal genomics researches. In this mini review, I have highlighted some basic knowledge about next generation sequencing technologies and its value for human health.

Keywords
Sequencing technologies; Exome; Genomic captures; Mitochondria
Introduction
The science is growing very fast and next generation sequencing technologies are currently the hottest topic in the field of human and animal’s enomgics researches [1]. Early efforts at sequencing genes were painstaking, time consuming, and labor intensive. This situation began to change during the mid-1970s, when researcher Frederick Sanger developed several faster, more efficient techniques to sequence DNA. Indeed, Sanger's work in this area was so ground breaking that it led to his receipt of the Nobel Prize in Chemistry in 1980 [2].
Next-generation sequencing (NGS) is a type of DNA sequencing technology that uses parallel sequencing of multiple small fragments of DNA to determine sequence [3]. In contrast to Sanger sequencing, the speed of sequencing and amounts of DNA sequence data generated with NGS, which is considered a “high-throughput technology”, are exponentially greater, and are produced at significantly reduced costs [4]. Third-generation sequencing uses parallel sequencing similar to NGS, but unlike NGS, third-generation sequencing uses single DNA molecules rather than amplified DNA as a template. Thus, thirdgeneration sequencing potentially eliminates errors in DNA sequence introduced in the laboratory during the DNA amplification process [5,6].
The starting material that provides a template for clinical NGS is double-stranded nuclear DNA. This can be obtained from a variety of cell types. In some cases, the DNA is further modified in the laboratory to remove non-coding regions when exome sequencing is performed [7,8].
NGS can be used to sequence every nucleotide in an individual’s DNA, or limited to smaller portions of the genome such as the exome or a preselected subset of genes [9,10]. Many second generation HTNGS platforms, are commercially available and the race for more additional platforms are continuously on the horizon [1]. The interpretation of the result of clinical NGS laboratories will reported as pathogenic, likely pathogenic, likely benign, benign and of unknown clinical significance [11]. The clinician and patient must be aware that the likelihood of receiving a result of “uncertain significance” is reasonably high, because the clinical implications of many variants are unknown [12].
As defined by the National Society of Genetic Counsellors, genetic counselling is the process of helping people understand and adapt to the medical, psychological and familial implications of genetic contributions to disease. This process helps to understand chance of disease occurrence or recurrence and provide platform for education about inheritance [13]. Consideration of NGS as a clinical tool for genetic diagnosis is appropriate in individuals for whom sequencing of a single gene is unlikely to provide a diagnosis especially in diseases with genetic heterogeneity [14,15]
NGS opens the entire spectrum of genomic alterations for the genetic analysis of complex traits. The main challenge in the fast growing HT-NGS technologies, is to cope with the analysis of vast production of sequencing database through advanced bioinformatics tools [1,16].
And Now, What is the Future NGS Technologies?
In the near future, HT-NGS technologies with ultra-deep sequencing of genomic DNA will be very helpful for medical purpose like analysis of the disease etiology and development in new drugs [1,17,18]. These researches will be of great value for genetic improvement of animal health and productivity [19].
HT-NGS technologies is a promising tool in the analysis of chromatin immunoprecipitation coupled to DNA microarray (ChIPchip) or sequencing (ChIP-seq), RNA sequencing (RNA-seq), whole genome genotyping, de novo assembling and re-assembling of genome, genome wide structural variation, mutation detection and carrier screening, detection of inherited disorders and complex human diseases, DNA library preparation, paired ends and genomic captures, sequencing of mitochondrial genome and personal genomics [1].
At the end, in the future we have a lot of hopes and a lot of questions.
Do we have the ability to control the pathophysiology of the diseases by understanding in depth their genetic background?
Do we have the ability in the near future to control cancer and prevent its occurrence?
Do we have the capacity in the near future to prolong life expectancy of mankind and control aging?
Finally, I still have a dream, which is an old one, and the answer will come in the near future with the help of sequencing technologies.
References
Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Relevant Topics

Recommended Conferences

Article Usage

  • Total views: 10301
  • [From(publication date):
    specialissue-2016 - Nov 21, 2017]
  • Breakdown by view type
  • HTML page views : 9735
  • PDF downloads : 566
 

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords