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Phosphotransfer Protein Ypd1p is Essential for Fludioxonil Action in Phytopathogenic Fungi | OMICS International
ISSN: 2165-8056
Fungal Genomics & Biology
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Phosphotransfer Protein Ypd1p is Essential for Fludioxonil Action in Phytopathogenic Fungi

Stefan Jacob*


Institut für Biotechnologie und Wirkstoff-Forschung gGmbH, Kaiserslautern, Germany

*Corresponding Author:
Stefan Jacob
Institut für Biotechnologie und Wirkstof f-Forschung gGmbH
Erwin Schrödinger Str .56, 67663 Kaiserslautern, Germany
Tel: +496313167221
E-mail: [email protected]

Received date: May 09, 2016; Accepted date: June 21, 2016; Published date: June 28, 2016

Citation: Jacob S (2016) Phosphotransfer Protein Ypd1p is Essential for Fludioxonil Action in Phytopathogenic Fungi .Fungal Genom Biol 6:140. doi:10.4172/2165-8056.1000140

Copyright: ©2016 Jacob S. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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Membranes of living cells are permeable to water but not for many dissolved particles. Consequently, cells have to cope with osmotic pressure in the environment. Most of the physiological processes within cells are extremely sensitive towards such changes. Microorganisms, especially fungi, cannot escape unfavourable changing environments, therefore relay on sensitive mechanisms to percept, transform and regulate the responses to these signals by modulating their cellular physiology. In this context, signaling pathways play a crucial role maintaining cellular homeostasis and one example is the high osmolarity glycerol (HOG) signaling pathway responsible for osmoregulation in fungi.

This pathway comprises a sensory phosphorelay system linked to a transferring MAPK cascade, and is well known in the model yeast Saccharomyces cerevisiae . In pathogenic fungi the HOG pathway differs accordingly, i.e in the number of histidine kinases in the phosphorelay system, and is yet not well understood. In the research article “High osmolarity glycerol (HOG) signalling in Magnaporthe oryzae : Identification of MoYPD1 and its role in osmoregulation, fungicide action, and pathogenicity” [1], we present the first functional analysis of an YPD1 -homolog in filamentous phytopathogenic fungi to date. We found that MoYpd1p is involved in osmoregulation and pathogenicity. MoYpd1p is also involved in the mode of action of the commercial fungicide fludioxonil. Thereby, we improved knowledge about the mechanism of action of fludioxonil by addressing the question, why the group III histidine kinase MoHik1p should be the target protein, although directed single gene inactivation of MoHIK1 is not lethal in M. oryzae .

The schemes of the HOG pathway presented in the manuscript display the difference between the modes of action of fludioxonil and single gene inactivation of the gene MoHIK1 . In case of an inactivation of the target protein MoHik1p , the phosphorylation pattern of the phosphorelay system may be maintained by MoSln1p and MoYpd1p or even by unknown factors, and the mutant strain is viable (Figure 1 left). The proposed mechanism of action of fludioxonil could be assumed to be an interference of fludioxonil with MoHik1p , leading to the inhibition of MoSln1p .


Figure 1:Comparison of the HOG signaling pathway in the Magnaporthe oryzae mutant strain ΔMohik1 (left) and the WT strain (right) upon treatment with fludioxonil. After a directed gene inactivation of MoHik1p the phosphorylation pattern of MoYpd1p and MoSsk1p can be partially rescued by MoSln1p. The response regulator MoSsk1p is phosphorylated under normal environmental conditions and the MAPK cascade MoSsk2p -MoPbs2p-MoHog1p is inactive. By contrast, fludioxonil treatment of the WT leads to the inhibition of MoHik1p and a subsequent dephosphorylation of all the signaling components of the phosphorelay system resulting in a complete dephosphorylation of MoSsk1p . Therefore, the MAPK cascade MoSsk2p-MoPbs2p-MoHog1p is hyperactivated, which results in cell death.

Therefore, phopshotransfer via MoYpd1p to MoSsk1p is inactivated. Consequently, MoSsk1p is dephosphorylated and MoHog1p hyperactivation causes cell death (Figure 1 right).


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