alexa Physico-Chemical Properties of Before and After Anaerobic Digestion of Jatropha Seed Cake and Mixed With Pure Cow Dung | OMICS International
ISSN: 2157-7048
Journal of Chemical Engineering & Process Technology
Like us on:
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Physico-Chemical Properties of Before and After Anaerobic Digestion of Jatropha Seed Cake and Mixed With Pure Cow Dung

Nisha Yadav1, Rajdeo Kumar1*, Laxmi Rawat1 and Sarita Gupta2
1 Forest Ecology and Environment Division, Forest Research Institute, Dehradun, India
2 CSWCRTI, Dehradun, India
Corresponding Author : Rajdeo Kumar
Forest Ecology & Environment Division
Forest Research Institute, Dehradun-248006 (Uttarakhand), India
Tel: 8591473231
E-mail: [email protected]
Received January 08, 2014; Accepted February 10, 2014; Published February 14, 2014
Citation: Yadav N, Kumar R, Rawat L, Gupta S (2014) Physico-Chemical Properties of Before and After Anaerobic Digestion of Jatropha Seed Cake and Mixed With Pure Cow Dung. J Chem Eng Process Technol 5:186. doi: 10.4172/2157-7048.1000186
Copyright: © 2014 Yadav N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related article at
DownloadPubmed DownloadScholar Google

Visit for more related articles at Journal of Chemical Engineering & Process Technology

Abstract

In this study, the biogas production potential of Jatropha seed cake (JSC) was assessed in the laboratory. The physico-chemical analysis data Total solids (TS%), Volatile solids (VS%), Organic carbon (OC%), Total nitrogen (N%), Total phosphorus (P%), Total potassium (K%), Moisture content (MC%) of the cakes confirmed that JSC is feasible of anaerobic digesters. By blending, JSC with cow dung in different ratios, the feedstock of the biogas digesters was prepared. From the nine treatments to analyze the changes in physico-chemical parameters of the fresh and digested slurry of moisture content is highest after digestion Treatment control-2, 15.8% and lowest before digestion (12.2%). But before digestion, Treatment control-2, TS is 98.2% and VS is 93.12% higher than after digestion. The organic nutrients content Treatment control-2 is C%, 27.37%, N% 2.67 and K % 1.98 of BD is higher than AD but P% 1.078% thus the phosphorous content had increased during the digestion process.

Keywords
Jatropha seed cake; Pure cow dung; Anaerobic digestion; Total solid; Volatile solid; Organic nutrient content
Introduction
Jatropha curcas is a perennial shrub to small evergreen tree of up to 6m height, adapted to all kinds of soil and does not demand any special nutritive regime [1]. It has been introduced in Africa and Asia and is now cultivated worldwide. In India, it was believed to be introduced by the Portuguese settlers during 16th century. It is a multipurpose, deciduous, small tree reported to be cultivated in drier regions of central and western parts of India. Recently, it has also been introduced in the northern and southern states of India. The plant is widely distributed and fits easily into agriculture system in the form of hedges, windbreak and erosion barrier or as a source of fire wood [2].
In recent years there are growing concerns about the utility of Jatropha curcas as a source of biofuel plantations in order to generate alternate source of energy. Jatropha seed cake is used as a fertilizer but the literature and data regarding the efficacy of improving crop yield or conditioning soil is limited. Jatropha curcas, commonly known as physic nut, belong to the euphorbiaceous family. It is a hardy plant, thrives on degraded land and requires limited amount of nutrients and water. The seeds contain about 300-350 gkg-1 oil, which can be used as a fuel directly or, in its trans esterified form, as a substitute for diesel. Large scale planting of jatropha has taken place or is planned in India, china, Madagascar, Myanmar and many other developing countries, with the aim of using the oil as biodiesel [3] in these countries the oil is produced from whole seeds using a screw press. The seed has a hard, black outer shell and kernel range from 350 to 400 gm.kg-1 and from 600 to 650 gm.kg-1 respectively.
The seed cake left as a by-product after oil extraction by screw press can contain as much as 500-600 gm.kg-¹ indigestible shells. The generation of biogas from these cakes would be a best solution for its efficient utilization. Anaerobic digestion has been considered as wasteto- energy technology, and is widely used in the treatment of different organic wastes for example: organic fraction of municipal solid waste, sewage sludge, food waste, animal manure, etc. Anaerobic treatment comprises of decomposition of organic material in the absence of free oxygen and production of methane, carbon dioxide, ammonia and traces of other gases and organic acids of low molecular weight. Anaerobic digestion provides an alternative option for energy recovery and waste treatment.
Materials and Methods
Statistics
The included Mean, Standard Error analysis was done using SPSS package and (Minitab 13 for windows software package) where used to analyze the Physico-chemical parameters VS, TS of feedstock and digestate, result of jatropha seed cake.
Experimental apparatus setup
Nine treatments in three replications were taken for the study. The treatment, T1 contained Jatropha seed cake (JSC) alone and the subsequent treatments consisted of cow dung (CD) and Jatropha seed cake in 1:1,1:2, 1:3, 1:4, 1:5, 2:1 and 3:1 for T2, T3, T4, T5, T6, T7 and T8 respectively. T9 with cow dung alone was taken as the control. The experiment was conducted during 84 days constant temperature around 35 ± 1°C for optimization of the biogas yield.
Hydraulic retention period
The optimum period for the economical gas production in batch fermentation depends largely on pattern of daily gas production and the pattern of changes in the calorific value of gas produced i.e. CH4 content. The study period in the experiment was 84 days.
Inoculum
The inoculum for the anaerobic digestion of non-edible oil seed cakes and cow dung will be prepared by composting the fresh cow dung under anaerobic condition for a period of five days in ambient temperature. The fresh cow dung contains active micro-organisms. A pinch of urea will be added in different treatments to supplement the nutrients so that it enhances the growth and multiplication of consortium of micro-organisms.
Anaerobic batch digestion assembly
Laboratory studies will be conducted by using 2L capacity conical flasks, and graduated cylinders, as batch digesters for carrying out the anaerobic digestion. The feed stocks will be diluted with the requisite amount of water so as to make 7-10% total solid concentration. The gas generated will be collected in inverted calibrated gas collection jar filled with water which will be partly immersed in water bath.
Measuring yield and quality of biogas
The gas production is measured by water displacement method whereas the quality, which is the percentage of methane from the biogas, is estimated by the displacement of sodium hydroxide, with a process held one next to the other. The gas volume produced in the anaerobic reactor was captured in a bottle filled with water, which was kept under pressure. When a gas bubble entered the bottle with water, the gas replaced the water, which was then forced out of the bottle into an empty bottle. The volume of water in the measuring cylinder thus resembled the gas production in the reactor. The displaced water is collected and then using a measuring cylinder its volume would be calculated. The displaced water indicates the total volume of biogas produced. Note that the gas coming out of the digester is stored in the displaced bottle. Adding back the displaced water to the displacement bottle would push out the biogas stored before; and passing it through 5% NaOH solution. The CO2 from the biogas would be retained in the solution whereas the methane would displace its equivalent volume of NaOH. Collecting the displaced solution and measuring its volume using a measuring cylinder would give the volume of methane from the produced biogas [4]. Hence, it would be possible to estimate the percentage of methane in the biogas, using the following simple equation.
Quantitative analysis of biogas yield
Cumulative yields of biogas (expressed in litres/kg of dry matter) from JSC and its admixtures with cow dung and the trends in biogas production are shown in Table 1.
The rate of biogas production from JSC was observed to be rapid as compared to cow dung during the second week of fermentation, the rate of production in the mixtures of cow dung and JSC increased substantially and biogas yield was faster than that from treatment with cow dung alone. JSC alone produced biogas of 76.5 L/kg dry matter (dm) within 14 days and there after its production attained steady pace. The trend pointed out a gradual increase to a maximum between 14 and 21 days, and then the gas production is maintained at a high level to the end of the experiment [5]. The total period taken for digestion was 84 days, beyond which the gas yield were more or less stopped as observed for all the treatments except CD:JSC in 2:1. But the treatment, CD: JSC in 2:1 ratio showed a steady increase in biogas generation throughout the study period and yielded the highest amount of biogas i.e. 500.15 L/kg dry matter followed by CD: JSC in 3:1, generating 420.7 L/kg dm. All the treatments except treatment with Jatropha seed cake alone have shown biogas potential higher than CD and produced 263, 256.34, 237.82, 273.54 and 276.94 L/kg dm of biogas by CD:JSC in 1:3,1:2,1:1,1:4 and 1:5 respectively. Compared to 184.34 L/kg from cow dung. This higher yield may be due to the synergistic action of microorganisms from the co-digestion process.
Sample preparation and feed solution
Jatropha curcas seed was collected from Dehradun. The seed cake was stored in a plastic bag at constant temperature around 35 ± 1°C and was blended prior to experiment. After blending the seed cake were mixed with tap water at ratios of JSC alone, 1:1, 1:2, 1:3, 1:4, 1:5, and 2:1, 3:1, CD alone by weight to create slurry.
Estimation of Volatile Solids, Total Solids, Moisture Content, Organic Carbon, Total Nitrogen, Total Potassium, Total Phosphorus
Total solid (TS)
The total solid content of the Jatropha seed cake (JSC) and cow dung (CD) can be determined by the procedure given by APHA [6]. A 10 g of freshly collected sample has to be taken in a disc and placed inside a hot air oven at 105°C for one hour. Then it is taken out and cooled to room temperature in desiccators and weighed. The percent of total solids is computed using the formula.
Where,
A= Mass of empty clean and oven dried silica crucible, g
B= Mass of silica crucible + sample, g
C= Mass of silica crucible + sample after oven drying, g
Volatile solids (VS)
The volatile solid content of sample can be determined by adopting the following procedure furnished by APHA [6]. A knows mass of residue obtained from the determined of the total solids is placed in a silica crucible and ignited in a muffle furnace at 550 ± 500°C for 30 minutes. Then the crucible is taken out of the muffle furnace, partially cooled in air, kept in desiccators for few minutes and then weighed. Volatile solids is computed using the formula given below,
Where,
A= Mass of empty clean and oven dried silica crucible, g
B= Mass of silica crucible + sample, g
C= Mass of silica crucible + sample after ignition, g
Moisture content
The moisture content will be determined using AOAC [7] method, about 15g of sample will be weighed in converted dish previously dried at 98-100°C, cooled in desiccator and weighed soon after reaching room temperature. Cover will be loosened and heated at 98-100°C to constant weight. At the end of drying, cover will be immediately tightened on dish, transferred to desiccator and weighed soon after reaching room temperature.
ΔSB = weight of dish and sample before drying.
ΔSA = weight of dish and sample after drying.
Carbon content
0.1g seed cake powder into a 500ml conical flask. Add 10ml of 1N potassium dichromate solution. Add 20 ml sulphuric acid and mix by gentle rotation for 1 minute taking care to avoid throwing seed cake powder up onto the sides of the flask. Let stand for 30minutes and dilute to 200 ml with distilled water. Add 10ml phosphoric acid, 0.2 g ammonium fluoride and 10 drops diphenylamine indicator. Titrate with 0.5 N ferrous ammonium sulphate solutions until the colour changes from dull green to a turbid blue. Add the titrating solution drop by drop until the end point is reached when the colour shifts to a brilliant green and prepare and titrate a blank in the same manner.
% Organic Matter=10[1(S/B)] × 0.67
% Organic Matter=10[1(S/B)] × 0.67
B=blank titration
Nitrogen content
Nitrogen content will be determined using (Kjeldahl method, 1950) half a gram powdered sample will be digested with concentrated H2SO4 in presence of digestion mixture (CuSO4 + K2SO4 + selenium powder in 20:10:1) till the digest give clear green colour. The digested sample will be further diluted carefully with distilled water to known volume. Then a known amount of aliquot will be transferred to distillation unit (Micro Kjeldahl apparatus) and liberated ammonia will get trapped in boric acid containing mixed indicator. Later, it will be titrated against standard H2SO4 and the amount of ammonia liberated indicator.
Potassium content
The potassium content of the samples will be estimated using the standard procedure as suggested by flame photometer method [8] which is explained below. For 10 ml of dried sample, 50 ml of 0.01 N ammonium acetate will be added. The sample gets leached with an additional 50 ml of ammonium acetate and made up the volume to 100 ml. The standard of potassium is fed to the flame photometer and the readings were noted down using K-filter. The procedure was repeated thrice and the average values are reported.
Phosphorus content
Plant and seed samples were digested with diacid mixture of HNO3 and HClO4 in the ratio of 9:4 and the extract was made to a definite volume. Total phosphorous was determined by vanado molybdate phosphoric acid yellow colour method at 730 nm [8].
Feedstock compositions of cow dung mixed with jatropha seed cake with different ratio were prepared as shown in Table 2.
Results and Discussion
In this study analysis of the different ratio of treatment composition of JSC and with CD showed that Table 3. The physico-chemical properties of the feedstock composition of the substrates before and after digestion in terms of moisture content, VS%, and TS% etc. Were analyzed and presented above Table 3.
Total solids (TS)
Different ratio of seed cake wastes were diluted and total solids of all treatments seed cake and cow dung which were held at for example one treatment TC-2, 98.2% showed a decrease after the retention period of 84 days which were found to be only 93.67%. Thus there was a 40% decrease in the TS% and thus indicated that the continuous feeding for digestion process is very small efficient in removing the total solids. Total solids are very important as far as the process is concerned. According to Abdulkarim et al. [9] at some point in the increase of the percent TS, no further rise in the volume of the biogas is obtained. Balsam [10] and Zennaki et al. found that the optimum solid content obtained for biogas production is in the range 7- 9%. Furthermore, Baserja [11] reported that the process was unstable below a total solids level of 7% (of manure) while a level of 10% caused an overloading of the digester. Itodo and Awulu [12] showed that slurries of higher TS concentrations were more acidic than that of lower TS concentrations.
Volatile solids (VS)
Volatile solids reduction occurred in the slurry during the digestion. The VS fed on an average basis into the digester were found to be all treatment for example one treatment like TC-2, 93.12%. The VS found after the digestion process was found to be 83.31%. Thus there was about 12% decrease in volatile solids. Volatile solids are the solids that are lost on ignition of the dry solids at 55°C centigrade. VS are responsible for biogas production. Since seed cake waste contains 93.12% of volatile solids thus it has a great potential of biogas production and can be used easily and potentially as a raw material for biogas production.
Moisture Content (MC)
The moisture content of the digestate increased from all treatment for example one treatment TC-2, 12.2% to 15.8% thus there was an increase (Table 3). According to Sadaka and Engler [13], water content is one of very important parameter affecting anaerobic digestion of solid wastes. There are two main reasons i.e., (a) Water make possible the movement and growth of bacteria facilitating the dissolution and transport of nutrient and (b) water reduces the limitation of mass transfer of non-homogenous or particulate substrate. In general, the moisture content of the digestate increased with increase in the amount of VS and TS reduction. The moisture content to be maintained for the degradation depends upon the type of the waste. In the present study final moisture content was at 15.8%.
Organic carbon content (OC%)
Organic carbon content reduction occurred in the slurry during the digestion. The OC fed on an average basis into the digester were found to be all treatment for example one treatment like TC-2, 27.37%. The OC found after the digestion process was found to be 24.78%. Percent carbon content in the feedstock was found to 24.80 in cow dung slurry and 48.80 in the JSC waste slurry. The reduction of C by anaerobic processes was therefore probably limited to the production of organic acids, H2 and CO2 by facultative bacteria [14]. Thus continuous process of feeding is also efficient in removing carbon content. The carbon compounds are converted to the CH4 and other gases like CO2. According to Richard and Wilkie [15,16], anaerobic bacteria do not or very slowly degrade lignin and some other hydrocarbons. In other word, the higher lignin content will lower biodegradability of waste. As food waste was used, it contains enough carbohydrates and less cellulose and lignin content, thus the removal of carbon content was fast.
Macronutrients content
In this study the amount of macronutrients content like K, N and P% which were present at the start of the digestion P is 0.066%, K is 0.86% and N is 1.890% of the waste. The macronutrients content were again analysed at the end of the study period (84 days) and it were found to be P is 1.078%, K is 0.78% and N is 1.24%. Thus the macronutrients content like phosphorus content had increased, potassium content had decreased and nitrogen content had decreased during the digestion process. Increase and decrease in the quality of macronutrient were recorded in the slurry; (Table 4). Phosphorous, Nitrogen and Potassium a macronutrient is very important as far as plant growth is considered. It is present in every living cell. It is very important to enhance process stability and maintain a stable operation for anaerobic digestion of municipal solid waste [17].
Conclusion
The supplementation of JSC to cow dung up to 75 per cent can be advantageously used for the economic biogas generation from a total solid concentration of 8-10 per cent. This shows that the mixing of these two materials in different proportions enhanced the gas production by utilizing more of the complex substrates than when used alone. The effect may be due to the synergistic action of a variety of cellulolytic and hydrolytic bacterial species in the breakdown of raw material [18]. The decline in gas production in treatment with JSC alone may be due to high lignin content. It is worth mentioning that slurry made of 2: 1cow dung and JSC was found the best among the various treatments in biogas generation. The admixtures of cow dung and JSC is a viable option for the energy production and for efficient management of jatropha seed cake which was otherwise creates environmental problems.
Acknowledgements
Authors are thankful to the Director FRI for his kind permission to publish this research article. We also acknowledged the help of Mr. Rajendra kumar for arrangement of all necessary chemicals, reagents etc.
References


















Figures at a glance

image   image   image   image
Table 1   Table 2   Table 3   Table 4
Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Recommended Conferences

  •  Annual Congress and Expo on Biofuels and Bioenergy
    April 16-17, 2018 Dubai, UAE
  • World Bioenergy Congress and Expo
    July 02-04, 2018 Berlin, Germany
  •  International Conferenceand Expo on Oil and Gas
    August 09-10, 2018 Madrid, Spain
  • International Conference on Chemical Engineering
    September 17-18, 2018 Vancouver, Canada

Article Usage

  • Total views: 12538
  • [From(publication date):
    March-2014 - Feb 22, 2018]
  • Breakdown by view type
  • HTML page views : 8609
  • PDF downloads : 3929

Review summary

  1. Amos Jacob Oladunni
    Posted on Jul 26 2016 at 9:53 pm
    this is a good job well done. am having a little problem after reading through experimental work. does it mean cowdung produce more biogas compare to jatropha cake? if yes I will like to know why and also the reason why jatropha itself is always stress being a very good source of biogas. I perform a similar project using the same substrates but I got reversed answer and other references also gave a different answer from yours. am an undergraduate student of university of Ilorin. Nigeria working on the same as my final year project. thanks
 

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version