Polyphenols Nanoencapsulation for Therapeutic Applications
Received Date: Mar 09, 2016 / Accepted Date: Apr 28, 2016 / Published Date: May 06, 2016
Natural polyphenols are valuable compounds present in plants, fruits, legumes, chocolate, tea, wine and marine organisms possessing scavenging properties towards radical oxygen species. These abilities make polyphenols interesting either for the treatment of various diseases like inflammation and cancer or for anti-ageing purposes in cosmetic formulations. Unfortunately, such compounds lack in long-term stability, are very sensitive to light, and often present a low water solubility and poor bioavailability. To overcome these limitations and enhance polyphenols therapeutic applications, nanotechnology-based delivery systems have been developed, and among all, nanoencapsulation represented a promising strategy. This review described a recent overview of physicochemical nanoencapsulated polyphenols focusing on the most representative molecules such as resveratrol, quercetin, epigallocatechin-3-gallate, and curcumin.
Keywords: Nanotechnology; Polyphenols; Drug delivery; Bioavailability
Polyphenols (PPH) are a large family of ubiquitous and varied molecules in the form of secondary metabolites of all vascular plants and several marine organisms. These natural compounds range from simple molecules to complex structures that have in common the presence of benzenic cycles bearing one or several hydroxy functions. These active principles play an important role in growth, reproduction, resistance to pathogens, predators and diseases . In particular, these phytochemicals contribute importantly to the color and organoleptic properties of plants while, in the case of marine organisms, they act in the antioxidative response of microalgae and cyanobacteria against UV exposure . Indeed, Klejdus et al.  showed that several classes of flavonoids, such as isoflavones, flavanones, flavonols and dihydrochalcones are found in microalgae and cyanobacteria despite of the fact that these organisms are evolutionary more primitive than terrestrial plants . In medicine, polyphenols contribute to the promotion of health and reduction in the risk of common chronic diseases  (Figure 1).
Several studies show an inverse correlation between the consumption of polyphenols and the risk of major illness such as cancer, cardiovascular diseases, type 2 diabetes mellitus, neurodegenerative diseases and osteoporosis . Such relationship is due to the PPH activity as potent effectors of biologic processes associated with the pathogenesis of human diseases. These effects also result from the ability of PPH to interact with proteins, enzymes, and membrane receptors modulating their activity in a specific way . Among their properties, the strong free radical scavenging action mainly due to PPH ability to donate hydrogen atoms or electrons is probably the most studied [6,7]. Antioxidant effect of polyphenols can be achieved by several mechanisms of action such as molecular complexation with pro-oxidant proteins, chelation of metal ions or direct trapping of Reactive Oxygen Species (ROS). Moreover, polyphenols are recognized also for their ability to beneficially affect inflammation (e.g. by edema inhibition), to upregulate detoxification pathways or to modulate cell‐signal transduction [8,9]. While PPH appear to have a dynamic interaction with gut microbiota, their efficacy in promoting health and reducing the risk of chronic diseases is dependent on their systemic bioavailability and metabolism. Generally, polyphenols have low bioavailability due to many intrinsic and extrinsic factors, including their chemical structure and molecular weight, low hydrosolubility, low stability in the gastrointestinal environment, extensive phase II metabolism and rapid elimination [10,11]. As a consequence, clinical applications of PPH are limited. To avoid these drawbacks, nanodelivery systems able to maintain the structural integrity of the bioactive molecules have been developed [12-14]. Numerous nanoencapsulation methods have been developed based on physical (e.g. spray drying), physicochemical (e.g. ionic gelation, hydrophobic interactions, etc.) and chemical (e.g. in situ polymerization) principles. This review highlights the recent progress in the strategies for physicochemical nanoencapsulation of epigallocatechin-3-gallate, resveratrol, quercetin, ellagitannin, ellagic acid, phlorotannins, oleuropein, hydroxytyrosol and curcumin chosen as most representative examples of polyphenols molecules.
Classification of Polyphenols
Thousands of polyphenolic compounds have grouped together in various classes, depending to the basic chemical skeleton variations, such as degrees of oxidation, hydroxylation, methylation, glycosylation and the possible connections to other molecules (Table 1 and Figure 2). A simple sorting divides polyphenols in four classes: flavonoids (including the subclasses anthocyanidins, catechins, flavones, flavonols, flavanones and isoflavones), coumarins, stilbenes and tannins, although other constituents, like chalcones and lignans exhibit polyphenolic structures. Flavonoids are C15 compounds with the structure C6-C3-C6 in which two benzene rings are linked together by a group of three carbons. The arrangement of the C3 group determines how the compounds are classified. Typical flavonoids have A-, B- and C-ring typically depicted with the A-ring on the left-hand side.
|Polyphenols||Chemical Structure||Examples||Sources(List is not Exhaustive)|
|Onions, broccoli, blueberries, red wine, tea|
|Parsley, celery, millet, wheat,
skin of citrus
|Grapefruit, orange, lemon, tomatoes, mint|
|Leguminous plants, soya|
|Apricot, cherry, grape, peach, apple, green and black tea, red wine, cider|
|Red wine, aubergines, cabbage, beans, onions, radishes, fruit in general|
|Coumarins||Ombelliferone, Aesculetin, Scopoletin||Tonka bean, chestnut, Melilotus
officinalis, Angelica officinalis
|Isocoumarins||Isocoumarin||Tonka bean, chestnut, Melilotus
officinalis, Angelica officinalis
|Tannins||Ellagitannin, Ellagic acid and Phlorotannins||Plants (pomegranate) and brown algae|
Table 1: Main classes of Polyphenols with structures and sources.
The A-ring originates from the condensation of three malonyl- CoA molecules, and the B-ring originates from p-coumaroyl-CoA. The A-ring in most of flavonoids is either meta-dihydroxylated or meta-trihydroxylated. Substituents on A- and B- ring, along with the arrangement of the ring C-, differentiate the anthocyanidins, catechins, flavones, flavonols, flavanones and isoflavones. Coumarins, having C6- C3 skeleton with an oxygen heterocycle as part of the C3-unit, include isocoumarins, with a similar structure but reversed position of the oxygen and carbonyl groups within the oxygen heterocycle. Stilbenes, with a C6-C2-C6 structure, chemically are diarylethene, featured with a central ethane double bond substituted with phenyl groups on each carbon atoms of the double bond. Tannins comprise a group of compounds with a wide diversity in structure that is characterized by the ability to bind and precipitate proteins and to form complex molecules. Typically, these molecules require at least 12 hydroxyl groups and five phenyl groups to function as protein binders. Tannins are classified in three subgroups: condensed tannins, hydrolysable tannins and complex tannins, according to the type of linkage between simple units .
Polyphenols Bioavailability and the use of Nano Vectors
Polyphenols low bioavailability is mainly due to the low absorption in the human gastrointestinal (GI) tract following consumption, extensive biotransformation within the gut and rapid clearance from the body (Figure 3) . In particular, many PPH are available as glycosylated compounds, and this form diminish their diffusion across barriers in the GI tract . Other polyphenols cannot be absorbed from enterocytes because they are unstable in the acidic condition of the stomach and in the alkaline status of the small intestine . Moreover, PPH are extensively transformed via phase II pathways, predominately methylation, glucuronidation and sulfation in the enterocytes of the small intestine, and then further metabolized in the liver, facilitating their quick excretion . A number of strategies have been used to increase the chemical stability or permeability of these species. These approaches usually rely upon the addition of chemical additives such as reducing agents to maintain the structure, the use of dissolving agents to increase solubility , the interaction with inhibitors of phase I and/or II enzymes to escape biotransformation , the addition of complementary ingredients such as lipids or protein , the conjugation with promoiety groups . More recently, encapsulation in nanovectors such as cyclodextrins, matrix systems, solid dispersions and liposomes has emerged as a novel strategy to improve poyphenols delivery, distribution and bioactivity . Such systems differ for the internal structure (core-shell-like or matrix) and the physical state of the encapsulated active substances. Moreover, the effective use of nanoparticles as drug delivery systems require polyphenol encapsulation efficiency (EE) of, at least, sixty per cent (the percentage of drug entrapment efficiency is calculated according to the formula: Experimental drug loading/Theoretical drug loading x100).
Systems used for Nanoencapsulation
Nanotechnology is a science involving the formation of particles with diameters ranging from 1 to 1000 nm and for which the end product exhibits properties or phenomena attributable to its dimensions . Nowadays, nanoencapsulation is an effective approach to improve solubility, minimize degradation process, reduce toxicity, and control the active absorption and biological response of polyphenols. Nanoencapsulation refers to several methods based on chemical, physical, and physiochemical principles. Chemical nanoencapsulation (e.g. interfacial and in situ polymerization methods) requires the polymerization of monomers at the interface of two immiscible substances through the addition of a cross-linker in the external phase . Physical processes (e.g. air-suspension method, pan coating, spray drying, spray congealing, micro-orifice system, etc.) involve the interaction of the vector material with the molecules to be encapsulated when both are aerosolized or atomized . Finally, physiochemical processes (e.g. coacervation, phase separation, complex emulsion,meltable dispersion and nanoprecipitation) form stable nanometer size drug nanosuspensions or nanoparticles through particle size reduction approaches . The process versatility combined with the ability to increase loading capacities, persistence at the target sites and permeation and retention effect, make physiochemical methods interesting approaches to enhance PPH pharmacologic action [13,14,26]. Recently, several studies analyze nanoparticle-mediated delivery of polyphenols, based on biodegradable and biocompatible polymers able to encapsulate polyphenols in nanostructures such as cyclodextrins, nanospheres, nanocapsules, solid lipid nanoparticles, liposomes and micelles (Figure 4) [27-46].
Cyclodextrins (CD) are a group of structurally related natural products formed during bacterial digestion of cellulose. They are structured as cyclic oligosaccharides consisting of (α-1,4)-linked α-Dglucopyranose units with lipophilic central cavity and hydrophilic outer surface. Specifically, the hydroxyl functions are orientated to the exterior and the central cavity is lined by the skeletal carbons and ethereal oxygens of the glucose residues. The natural α-, β- and γ- cyclodextrins (αCD, βCD and γCD) consist of six, seven, and eight glucopyranose units, respectively. These molecules have a limited aqueous solubility . Effective NanoDDS are obtained using watersoluble cyclodextrin derivatives such as hydroxypropyl βCD and γCD, randomly methylated β-cyclodextrin (RMβCD), and sulfobutylether β-cyclodextrin sodium salt (SBEβCD) . CD form complexes with molecules through inclusion into the CD cavity via van der Waals connections, hydrophobic interactions or hydrogen bonds. This complexation is enhanced when CD and drug have opposite charge and the temperature is low [29,30]. Another type of nanosized vectors are nanospheres (NS), structured with hydrophobic chains forming the inner part of the spheres and hydrophilic portions oriented on the surface. These NanoDDS have homogeneous solid matrices in which the polymer chains arrange in a “frozen” state phase-separated from the bulk solution . Nanospheres allow a fine tuning of their properties through the use of different shell materials such as Poly-lactic acid (PLA), Poly-glycolic acid (PGA), Poly-lactic-co-glycolic acid (PLGA), poly ε-caprolactone (PCL), Chitosan (CS), Polyethylene glycol (PEG) and Eudragit (anionic copolymers based on methacrylic acid and methyl methacrylate). These polymers are widely used for the preparation of NS due to their biocompatibility and biodegradability . Drugs are dissolved, entrapped, encapsulated, chemically bound or adsorbed to the constituent polymer matrix in order to be effectively delivered to the site of action . Nanocapsules (NC) have similar composition but exhibit a core-shell structure in which the drug is confined within a cavity surrounded by a polymer membrane . Nanocapsules can carry the active substance also on their surfaces or imbibed into the layer . The cavity contains the active substances both in liquid or solid form . Solid Lipid nanoparticles (SLNs) are vectors composed of high melting point lipids, as solid core, coated by aqueous surfactants. Examples of core lipids are fatty acids, acylglycerols and waxes, whereas phospholipids, sphingomyelins, bile salts and sterols are utilized as stabilizers . SLNs have high biocompatibility, high bioavailability, physical stability, protection abilities of incorporated labile drugs from degradation, excellent tolerability, prevention of problems related with multiple routes of administration, avoidance of the use of organic solvents during the preparation and absence of problems concerning large scale production and sterilization [38,39]. However, common disadvantages of SLNs are particle growth, unpredictable gelation tendency, uncertain diffusion of the drug within the lipid matrix of the vector and unexpected dynamics of polymorphic transitions . Liposomes (LS) are drug delivery systems that form spontaneously by hydration of lipid powder in aqueous medium produced from cholesterols, non-toxic surfactants, sphingolipids, glycolipids, long chain fatty acids and membrane proteins . Liposomes have particle sizes ranging from 30 nm to several micrometers and consist of one or more lamellaes (phospholipidic bilayer membranes) surrounding aqueous units where the polar head groups are oriented in the pathway of the interior and exterior aqueous phases . Self-aggregation of polar lipids is not limited to conventional bilayer structures, but they may self-assemble into various types of colloidal particles depending on molecular shape, temperature, and dispersion conditions . Micelles (MC) are colloidal dispersions, with particle size ranging between 5 to 100 nm that form spontaneously at certain concentration and temperature values from amphiphilic agents. MC formation is driven by the decrease of free energy in the system because of the removal of hydrophobic fragments from the continuous phase, and the reestablishing of hydrogen bond network in water. Moreover, additional energy results from the formation of Van der Waals bonds between hydrophobic blocks in the core of the formed micelles. Hydrophobic fragments of amphiphilic molecules form the core of micelles, while hydrophilic portions the shells . MC possesses high stability, good biocompatibility, and is able to solubilize a broad variety of poorly soluble pharmaceuticals . Examples of amphiphilic agents used to form MC are Pluronic (Plu), Poly (ethylene glycol) (PEG), Poly (D,Llactide- co-glycolide) (PLGA) and Polycaprolactone (PCL) .
Nanoparticles as Potential Delivery Systems of Polyphenols
Epigallocatechin-3-gallate is a water-soluble flavanol found predominately in green tea leaves (Camellia sinensis) (Figure 5) that acts in the prevention of some forms of cancer, cardiovascular diseases, type 2 diabetes mellitus and osteoporosis. Epigallocatechin-3-gallate is highly susceptible to degradation in the intestinal milieu and via oxidative processes. Efforts to promote the intake, enhance the stability, and increase bioavailability of EGCG are directed to incorporate this flavanol into nanosized delivery vectors (Table 2).
|Nanovectors||Type of Delivery Systems||References|
|Polyester nanoparticles||Poly(L-lactide)-poly(ethylene glycol), (PLA-PEG) nanoparticles|||
|PLGA-PEG nanoparticles functionalized with the prostate-specific membrane antigen (PSMA) inhibitor on the surface|||
|PLGA biodegradable nanoparticles|||
|Serum albumin nanoparticles||Bovine serum albumin (BSA) nanoparticles|||
|Carbohydrate matrix||Carbohydrate matrix composed of maltodextrin (60%) and gum arabic (40%)|||
|Chitosan nanoparticles||[53, 54]|
Table 2: Nano delivery systems for epigallocatechin gallate.
Siddiqui et al.  reported the encapsulation of EGCG in poly (L-lactide)-poly (ethylene glycol), (PLA-PEG) nanoparticles and assessed its efficacy against human prostate cancer (PC3) cells both in vitro and in vivo. The results showed that encapsulated EGCG retained its biological effectiveness with an over 10-fold dose advantage form exerting its efficacy in the inhibition of PC3 proliferation. Moreover, PLA-PEG nanoparticles were biocompatible and permitted the control of the time and rate of polymer degradation. Epigallocatechin-3-gallate was also incorporated in bovine serum albumin (BSA) nanoparticles (with a mean particle size of 200 nm) and their effect evaluated against PC3 cells . In this work, PC3 cells lethality was positively correlated with the nanoparticles uptake amount. The specific targeting to prostate cancer cells was obtained also with Poly (lactide-co-glycolide)- Poly(ethylene glycol) (PLGA-PEG) nanoparticles encapsulating EGCG and functionalized with the prostate-specific membrane antigen (PSMA) inhibitor . Other PLGA-based nanovectors for EGCG were synthesized by Italia et al.  with a loading efficiency of 70 % and high antioxidant efficiency in vivo. These nanoparticles, given by oral administration, acted 3 times more quickly of solutions of free epigallocatechin-3-gallate administered parenterally. A further nanosized delivery systems encapsulating epigallocatechin gallate is constituted by carbohydrate matrix composed of maltodextrin (60%) and gum arabic (40%) with EE of 85% . These particles were able to inhibit steps of the tumorigenesis process. Smith et al.  immobilized EGCG on lipid-coated nanoparticles with a bioavailability, after encapsulation, increased twice-fold compared to that of the free form. Epigallocatechin gallate inside the membrane preserved its antioxidant activity and blocked the production of hepatocyte growth factor (HGF) from cancer cell lines MBA-MD-231. On these bases were prepared also gelatin nanoparticles loaded with EGCG with an interesting inhibitory effect on HGF-induced cell scattering . Finally, epigallocatechin- 3-gallate was encapsulated into chitosan nanoparticles (sizing 165 nm and exhibiting a zeta potential of 33 mV) by Dube et al.  in order to evaluate the ability of chitosan tripolyphosphate nanoparticles to increase EGCG stability and bioavailability. They found that EGCGchitosan nanoparticles incubated in alkaline solution took more time to degrade to 50% of the initial level, compared to pure epigallocatechin. Moreover, Dube et al. found that chitosan tripolyphosphate EGCG exhibited a 1.8-fold greater absorption rate than the same dose of free EGCG in ex vivo mice experiments and that chitosan NP increased the relative oral bioavailability by 1.5-fold compared to the same dose of free EGCG in an in vivo absorption experiment on mice .
Quercetin is a semi-lipophilic flavonol ubiquitous in plants (Figure 6). In medicine, dietary supplementation of QC is promoted for prevention and treatment of cancer and this active principle is now largely utilized as a nutritional supplement and as a phytochemical remedy for a variety of diseases like diabetes, obesity, circulatory dysfunction, inflammation and mood disorders. Quercetin has a strong antioxidant activity which potentially enables it to quench free radicals from forming resonance-stabilized phenoxyl radicals but, like most flavonoids, it is extensively transformed via phase II metabolism for elimination from the body . Nanosized vectors encapsulating quercetin were developed to improve its oral bioavailability and to enhance its antioxidant and anti-inflammatory action (Table 3).
|Nanovectors||Type of Delivery Systems||References|
|Solid lipid nanoparticles||SLNs composed of soya lecithin, Tween-80 and PEG|||
|Acrylic nanoparticles||Aminoalkyl methacrylate copolymers nanoparticles|||
|Polyester nanoparticles||PLGA nanoparticles||[60 - 63]|
Table 3: Nano delivery systems for Quercetin.
Li et al.  reported the synthesis of solid lipid nanoparticles of 155 nm of size composed of soya lecithin, Tween-80 and PEG that encapsulate QC, with EE of 91%. These nanoparticles were able to increase the relative oral bioavailability of quercetin by 5.7 fold as compared to the free form. Lipid-coated Nano capsules were reported by Barras et al. , with a solubility 100 times higher respect to free quercetin, stable for more than ten weeks and with no degradation product being detected. Wu et al.  prepared nanosized QC delivery systems with aminoalkyl methacrylate copolymers sized 82 nm and with encapsulation efficiency of 99%. They resulted in a in vitro radical scavenging activity of quercetin nanoparticles toward di(phenyl)- (2,4,6-trinitrophenyl)iminoazanium (DPPH) radicals and superoxide anion enhanced by 883- and 1377- fold as compared to the free form. However, in recent years, an increasing number of works reported the synthesis of PLGA nanoparticles able to encapsulate QC. Indeed, Pool et al.  reported that quercetin encapsulated within PLGA displayed a more potent antioxidant action against peroxyl radicalinduced lipid peroxidation and a greater capacity for chelating activity toward transient metals than free QC. Ghosh et al.  evaluated the antioxidant activity of a single pre-treatment of quercetin encapsulated into PLGA nanoparticles, administered orally to female rats, in the protection against injury from ROS produced in liver and brain tissue subsequent to a subcutaneous injection of arsenite. QC-PLGA nanoparticles provided full protection against the arsenite induced ROS injury, while free quercetin was ineffective. The same group reported a synergy between QC and meso-2,3-dimercaptosuccinic acid (a hydrophilic arsenic chelator), encapsulated together into PLGA nanoparticles, in the protection against arsenic induced damages . In a rat model, Chakraborty et al.  examined the potency of a single dose of quercetin-PLGA nanoparticles administered orally prior to alcohol induced gastric ulcer in the protection against oxidative damages, demonstrating that QC-PLGA nanoparticles prevented 90 % of alcohol-induced ulceration as compared to the 20% of the free quercetin. QC encapsulated into PLA nanoparticles had increased antioxidant activity along with a slow and total release after 72 h, useful for potential therapeutic applications . Similarly, Wu et al.  synthesized Eudragit-polyvinilalchool quercetin-loaded nanoparticles with particle size of 85 nm, good polydispersity, drug loading of around 99% and enhanced antioxidant activity. Lastly, QC and myricetin in 2-(hydroxy-propyl)-ß-cyclodextrines (HP-ß-CD) had improved bioavailability .
Resveratrol, chemically known as 3,5,4’-trihydroxystilbene, is a naturally occurring polyphenol produced by a wide variety of plants in response to injury, UV Irradiation, ozone exposure and fungal attack (Figure 7) . This polyphenol is an antioxidant , antiinflammatory , anticancer , cell cycle inhibitor , antiaging , neuroprotector  and cardioprotector  agent with application in the treatment of obesity and diabetes . Moreover, it is used to stabilize polyester films for packaging and potential biomedical applications . However, resveratrol has rapid and extensive metabolism  that affects its body distribution and bioavailability. Further, there is a significant person-to-person variability in drug absorption and metabolic processes depending on the hepatic function and on the metabolic activity of the local intestinal microflora . To overcome these problems, in the recent years, several nano-drug delivery systems were synthesized (Table 4).
|Nanovectors||Type of Delivery Systems||References|
|Liposomes||Cholesterol, dicetyl phosphate and lecithin liposomes|||
|Soy phosphatidylcholine liposomes|||
|Liposomal resveratrol formulation for intravenous administration|||
|Proliposomal formulation containing distearoylphosphatidyl choline (DSPC)|||
|Polyester nanoparticles||PEG-PCL nanoparticles|||
|Eudragit RL 100 nanoparticles|||
|Carbohydrate matrix||Carboxymethyl chitosan nanoparticles|||
|Solid Lipid nanoparticles||Stearic acid and Phospholipon® 90G SLNs|||
|Cetylpalmitate and polysorbate 60 SLNs|||
|Glycerylbehenate-based solid lipid nanoparticles|||
|Cyclodextrins||Cyclodextrins based nanosponges|||
Table 4: Nano delivery systems for Resveratrol
A good example is the liposomal formulation containing RE, with a good encapsulation efficiency of around 70% . PEG-PCL resveratrol nanoparticles had enhanced loading efficiency and higher cytotoxicity to malignant glioma cells, compared with free resveratrol, and a good cellular uptake occurring by endocytosis . Singh and Pai  reported sustained release of trans-resveratrol from orally administered PLGA nanoparticles (drug encapsulation efficiency more than 78%, with a particle size of about 170 nm). RE was detected in the rat plasma for up to 4 days with higher concentration in the systemic circulation and in the organs rich in mononuclear phagocyte system. The same authors encapsulated resveratrol in Eudragit RL 100 nanoparticles with a drug incorporation efficiency of 84% and the average size 180 nm. The in vivo studies of these vectors, in a rat model, showed that nanoparticles exhibited prolonged plasma levels up to 16 h, maintaining drug levels in the liver, spleen, heart, lungs, kidney and brain up to 24 h in comparison with free drug being cleared within 6 h . Zu et al.  reported carboxymethyl chitosan nanoparticles as potential carrier for resveratrol. These nanoparticles (sized 155 nm and showing an encapsulation efficiency of 44%) improved the solubility of resveratrol, thereby greatly affecting the antioxidant activity of the drug. In vivo biodistribution study indicated higher body localization of drug loaded carboxymethyl chitosan nanoparticles, in comparison to free resveratrol solution in phosphate buffered saline (PBS) . Additionally, were synthesized resveratrol loaded Solid Lipid nanoparticles with a controlled release profile, due to an initial burst release of 40% caused by the active principle associated with the particle shell, and a subsequent prolonged release of the drug located in the lipid matrix. In this system, the efficiency of the cellular uptake depended on the molecular interactions with the biological membrane organization, lipid rafts and the actin cytoskeleton invaginations for the receptor mediated entrance. Moreover, SLNs were able to carry RE to the nuclear target site . Resveratrol loaded SLNs were also prepared by Pandita et al.  with a drug incorporation efficiency of 89% and average diameter of 134 nm. The drug delivery system showed prolonged release in vitro up to 120 h in a Wistar rat model, enhancing plasma bioavailability compared to free drug suspension. Other resveratrol-SLNs were produced using Polysorbate 60 as surfactant, with an entrapment efficiency of 70% and the particle size ranged between 150 and 250 nm: the drug release in simulated gastric fluid (SGF) was of 8% at 3 h . Jose et al.  checked the brain targeting ability of glyceryl behenate-based solid lipid nanoparticles to utilize the potent anticancer properties of resveratrol. The in vivo biodistribution study using Wistar rats demonstrated that these SLNs significantly increased the brain concentration of RE. Moreover, these DDS had lower distribution to other tissues, proving the targeting abilities of this system. Resveratrol loaded soy phosphatidylcholine liposomes were synthesized by Pando et al.  to investigate the ex vivo percutaneous absorption of RE. Results indicated high cutaneous accumulation and low transdermal delivery of the drug. Moreover, Coimbra et al.  designed liposomal resveratrol formulation for intravenous administration. The encapsulation process gave protection against trans–cis isomerization, with 70% trans-resveratrol still present after 16 min of UV light exposure compared to 10% when resveratrol is exposed in its free form. In addition, in vivo, intravenous injection of 5 mg/kg body weight of resveratrol in nude BALB/c female mice with subcutaneous head and neck squamous cell carcinoma led to a significant reduction in tumor volume . Basavaraj et al.  produced resveratrol-loaded proliposomal formulation with entrapment efficiency of 20–23% and zeta potential of −22 mV containing distearoyl phosphatidyl choline (DSPC) with or without cholesterol. Faster drug release was observed in formulation without cholesterol and the release increased as the amount of DSPC in the formulation was enhanced. Lu et al.  produced resveratrol loaded micelles in which polycaprolactone (PCL) constituted the hydrophobic core and poly (ethylene glycol) (PEG) was the hydrophilic shell of micelles. This system showed a protective effect on adrenal gland PC12 cells against superoxide-induced damage during the phenomenon of oxidative stress. Finally, cyclodextrins-resveratrol complexes were used to increase the concentration of the polyphenol in aqueous solution, while maintaining its biological activity. For example, spherical CDbased nanosponges showed increasing solubility and stability, together with good drug encapsulation efficiency, compared to free RE .
Ellagitannin (ET), Ellagic acid (EAC) and Phlorotannins (PHT)
Ellagitannin, Ellagic acid and Phlorotannins are examples of the phenolic class of tannins, widely diffused in plant and marine organisms (Figure 8). In fact, ET and EAC are most present in the pomegranate (Punica granatum), a fruit-bearing shrub that originated in the region from Iran to northern India, while phlorotannins are isolated from algae. Ellagitannin and Ellagic acid decrease heart disease risk factors, affect LDL oxidation, macrophage oxidative status, foam cell formation [88,89] provoke the reduction of systolic blood pressure by inhibiting serum angiotensin-converting enzyme  and prevent cancer. These tannins show free-radical scavenging properties [91,92] and selectively inhibit the growth of breast, prostate, colon, lung tumors, and skin cancer [93,94]. Similar properties are found in phlorotannins, present in brown algae, formed by the polymerization of phloroglucinol. Specifically, the phlorotannins eckol, phlorofucofuroeckol A, dieckol and 8,8’-bieckol are isolated from the Laminariaceous brown algae Eisenia bicyclis (Kjellman) Setchell, Ecklonia cava Kjellman and Ecklonia kurome Okamura . All these tannins have limited therapeutic applications due to low water solubility, inadequate permeability , poor absorption and instability . Nanosized drug delivery systems mask the active substances inside the nanoparticulate network, thus preventing degradation and increasing bioavailability (Table 5).
|Nanovectors||Type of Delivery Systems||References|
|Carbohydrate matrix||Gelatin nanoparticles incorporating partially purified pomegranate (PPE) ellagitannins|||
|Polyester nanoparticles||PLGA nanoparticles|||
|Liposomes||Lecithin unilamellar vesicles|||
Table 5: Nano delivery systems for Tannins.
For example, gelatin nanoparticles incorporating partially purified pomegranate (PPE) ellagitannins showed a good loading efficiency and the capacity of inducing a late stage of apoptosis and necrosis in the human promyelocytic leukemia cells (HL-60) . Similar results were obtained with Chitosan nanoparticles containing ellagic acid  and with Ellagic acid-loaded PLGA nanoparticles . In particular, Ellagic acid-Chitosan nanoparticles were spherical shaped with an average particle size of 176 nm, showing a drug-encapsulation and loading-efficiency of 94% and 33%, respectively. The in vitro drug release profile in the PBS medium showed sustained release of EAC from chitosan nanoparticles. Further, the therapeutic efficacy of Ellagic acid-Chitosan nanoparticles in human oral cancer cell line (KB) exhibited significant cytotoxicity in KB cells in a dose-dependent manner with a very low IC50 value compared to the free EAC .Ellagic acid-loaded PLGA nanoparticles had a rapid initial release of EAC in pH 7.4 phosphate buffer, followed by a slower sustained release . Further, the authors tested the influence of the stabilizers DMAB and PVA on the size, loading efficacy, release kinetics in PBS, stability, cytotoxic activities and in situ intestinal permeability of these nanoparticles . Same study was conducted also on PLGA-PCL ellagic acid nanoparticles . Both resulted on improved tannin bioavailability with potential therapeutic application due to the oral administration of smaller quantity of nanoparticles, compared to the free drug. Shirode et al.  synthesized poly (D,L-lactic-co-glycolic acid)–poly(ethylene glycol) (PLGA–PEG) nanoparticles, with an average diameter of 150-200 nm, loaded with pomegranate extract or with the individual polyphenol components (e.g. punicalagin or ellagic acid). Synthetized nanoparticles showed a 2- to 12-fold enhanced antiproliferative effect on MCF-7 and Hs578T breast cancer cells compared to free extracts . Similarly, brown algal phlorotannins were encapsulated in lecithin unilamellar vesicles prepared by the extrusion method. These nanovectors maintained their activity on lipid peroxidation inhibition and radical scavenging activities and presented the advantage of the improved stability .
Oleuropein (OR) and Hydroxytyrosol (HYT)
Oleuropein and Hydroxytyrosol are phenolic compounds naturally present in olive fruits and leaves (Figure 9) . Indeed, olive leaf extracts are rich in oleuropein, demethyloleuropein, oleuroside, verbascoside, non-glycosidic secoiridoids and ligstrosides as well as several flavonoids and biflavonoids. Also olive oil vegetation water (or olive mill waste water), obtained by centrifugation or sedimentation of the olive oil, contains these organic polyphenols. Such compounds have several pharmacological properties, including antioxidant, anti-inflammatory, anti-atherogenic, anticancer, antimicrobial, antiviral and cardioprotective effects. Oleuropein and hydroxytyrosol exhibit similar free radical-scavenger ability, but with different action mechanisms. In vitro studies, in a model system consisting of dipalmitoylphosphatidylcholine/linoleic acid unilamellar vesicles (DPPC/LA LUVs) and a water-soluble azo compound as a free radical generator (LP–LUV test)  revealed that hydroxytyrosol can serve as scavenger of aqueous peroxyl radicals near the membrane surface, while oleuropein acts also as a scavenger of chain-propagating lipid peroxyl radicals within the membranes. The pharmacologic activity of these active principles is enhanced by the use of nanotechnology as reported in Table 6.
|Nanovectors||Type of Delivery Systems||References|
|Serum albumin nanoparticles||Magnetic bovine serum albumin (BSA) nanoparticles|||
|Carbohydrate matrix||Chitosan nanoparticles|||
|Chitosan nanoparticles incorporated into an aqueous cream||[107,108]|
|Polyester nanoparticles||mPEG-PLGA nanoparticles|||
|Micelles||whey protein concentrate (WPC) and pectin complexes|||
Table 6: Nano delivery systems for Oleuropein and Hydroxytyrosol.
Haghighi et al.  developed oleuropein-magnetic bovine serum albumin (BSA) nanoparticles with an initial burst and a sustained release. Hussain et al.  administered chitosan co-loaded hydrocortisone (HC) and hydroxytyrosol nanoparticles to provide additional anti-inflammatory and antioxidant benefits in the treatment of atopic dermatitis (AD). The co-loaded nanoparticles showed different particle sizes, zeta potentials, loading efficiencies, and morphology, when the pH of the chitosan solution was increased from 3.0 to 7.0. Moreover, they significantly increased the permeation of the drugs and showed higher epidermal and dermal accumulation of HC compared to commercial hydrocortisone formulations. In vivo studies resulted in the efficient control of transepidermal water loss, intensity of erythema and dermatitis index . Similarly, a patent by Katas used  hydrocortisone and hydroxytyrosol loaded chitosan nanoparticles as local treatment of atopic dermatitis. Such nanovectors gave reduced side effects, increased efficiency of delivery, antibacterial properties and enhanced transepidermal penetration. Moreover, Siddique et al.  produced HC-HT co-loaded chitosan nanoparticles (HC– HT CSNPs) of 235 nm in size and with zeta potential +39.2 mV incorporated into aqueous cream (vehicle) to treat atopic dermatitis. This formulation was investigated in vivo, in albino Wistar rats, for acute dermal toxicity, dermal irritation and repeated dose toxicity. The results proved that HC–HT CS nanoparticles did not cause neither skin irritation, nor adverse-effect with respect to body weight, organ weight, feed consumption, blood hematological and biochemical, urinalysis, and histopathological parameters, when the HC–HT CS were used for 28 days at a dose of 1000 mg/body surface area per day. This study demonstrated that nanoencapsulation significantly reduced the toxic effects of HC. Qingxia Guan et al.  investigated the therapeutic efficiency of monomethoxy polyethylene glycol-poly (lactic co-glycolic acid) (mPEG-PLGA) nanoparticles co-loaded with syringopicroside and hydroxytyrosol prepared using a nanoprecipitation method. In particular, they analyzed the parameters of in vivo pharmacokinetics, biodistribution, fluorescence, endomicroscopy and cellular uptake. This vector (92 nm with a narrow polydispersity and a negative zeta potential of -24.5 mV) showed an encapsulation efficiency of ~ 33% and drug loading of 12%, that allows persisting drug plasma concentrations while the nanoparticles moved gradually into the cell, thereby increasing the available quantity. The in vitro effect resulted in the liver hepatocellular carcinoma (HepG2.2.15) cells proliferation inhibition . Olive leaf extract containing oleuropein and hydroxytyrosol was encapsulated in β-CD, increasing the water solubility and antioxidant capacity of the encapsulated polyphenols . Similarly, López-García et al.  studied the complexation of hydroxytyrosol with β-CD or HP-β-CD. Only β-CD appeared to be very strong photo-protectors of polyphenolic compounds subjected to ultraviolet radiation (λ=254 nm). Finally, Mohammadi et al. showed that water/oil/water emulsion with whey protein concentrate (WPC) and pectin complexes of olive leaf phenolic compounds result in a high stability and controlled release of the encapsulated compounds .
Curcumin is the principal polyphenol of the curcuminoid class of the Indian spice Curcuma Longa Linn., a plant typically grown and used in Southeast Asia (Figure 10) . Curcumin lowers cholesterol, reduces platelet aggregation, inhibits the proliferation of cancer cells and improves digestion by increasing the flow of bile from the gallbladder . Moreover, it has immunomodulatory, antiangiogenic and neuroprotective actions, antioxidant and anti-inflammatory abilities and the capacity to modulate the activity of several key transcription factors, cytokines, growth factors, kinases and other enzymes . The activity of CU is correlated with the inhibition of the inducible nuclear factor kappa B (NF-kB) activation, and the suppression of cancer cell proliferation . In addition, the clinical efficacy for rheumatoid arthritis, psoriasis, and postoperative inflammation of Curcumin is due to the reduction of NF-kB, cyclooxygenase 2 (COX2) and proinflammatory cytokines . However, the pharmacological potential of CU is hampered by its low solubility, bioavailability, and stability . In fact, Curcumin is sparingly soluble in water at acidic or neutral pH and slightly soluble under alkali conditions. For another CU is unstable in the gut and trace amounts of curcumin that pass through the gastrointestinal tract are rapidly degraded . Nanotechnology permits to enhance the bioavailability of CU through the encapsulation of the drug in the hydrophobic domains of various types of nanovectors (Table 7).
|Nanovectors||Type of Delivery Systems||References|
|Serum albumin nanoparticles||Human Serum Albumin nanoparticles||[120,121]|
|Micelles||Polyethylene glycol (PEG)-derivatized FTS (S-trans, trans-farnesylthiosalicylic acid)-based nanomicellar system|||
|Diblock Copolymer Micelles made of oleoyl chloride and methoxy polyethylene glycol 2000|||
|Carbohydrate matrix||Chitosan–starch (BDMCA–CS) nanocomposite|||
|Polyester nanoparticles||PLGA nanoparticles|||
|Poly-(allylamine hydrochloride) (PAH), poly-(sodium 4-styrenesulfonate) (PSS) and chitosan nanocapsules|||
|Solid Lipid nanoparticles||Dioctyl sodium sulfosuccinate and Poloxamer 188 SLNs|||
|Transferrin-mediated solid lipid nanoparticles||[37,38]|
Table 7: Nano delivery systems for Curcumin.
β-Cyclodextrin-curcumin (CD-CUR) nanovectors inhibited telomerase gene expression in breast cancer cells. CD-CUR lowered the gene expression of telomerase more than free curcumin in breast cancer cells (T47D) in a time and dose dependent manner . Similarly, Human Serum Albumin (HSA) possessed two affinity sites for curcumin  and literature studies revealed that Curcumin- HSA complexes had greater therapeutic effect than free CU, with no observable toxicity . Self-assembled micelles containing Curcumin spontaneously formed in aqueous phase using Pluronic (Plu), poly(ethylene glycol) (PEG), Poly (D,L-lactide-co-glycolide) (PLGA), or polycaprolactone (PCL) with improved drug stability [44,122]. For example, Chen et al.  formulated curcumin in polyethylene glycol (PEG)-derivatized FTS (S-trans, trans-farnesylthiosalicylic acid)-based nanomicellar system. This nanovector had small size of around 20 nm. The nanomicellar curcumin demonstrated enhanced cytotoxicity towards several cancer cell lines in vitro. Moreover, intravenous application of curcumin-loaded micelles led to a significantly more effective inhibition of tumor growth in a syngeneic mouse breast cancer model (4T1.2) than free Curcumin. Similarly, Alizadeh et al.  synthesized diblock copolymer micelles made of oleoyl chloride and methoxy polyethylene glycol 2000 effective in inhibiting mammary and hepatocellular carcinoma cells proliferation in vitro. Moreover, tumor growth in micelles-treated mice was significantly suppressed and/or almost completely stopped at the end of the treatment . Also, polymeric nanoparticles were structurally effective for embedding the water-insoluble curcumin. As the micelles, the most commonly used polymers included polylactic acid, polyglycolic acid, copolymer lactic acid/glycolic acid, polyethylene glycol, chitosan or a blend of these . For example, bis-demethoxy curcumin analog loaded Chitosan– starch (BDMCA–CS) nanocomposite particles were developed by Subramanian et al.  by ionic gelation method. The entrapment efficiency and drug loading capacity were high and the in vitro-drug release showed a slow and sustained diffusion controlled release of the drug. Curcumin loaded PLGA nanoparticles; described by Verderio et al.  released intracellularly CU in a time and dose dependent manner at low drug concentration, yielding cell proliferation inhibition by G2/M cell arrest. Poly-(allylamine hydrochloride) (PAH), poly-(sodium 4-styrenesulfonate) (PSS) and chitosan nanocapsules loaded with curcumin were fabricated by Goethals et al. around a solid core/mesoporus (SC/MS) structure. These nanovectors resulted in high cytotoxicity in breast cancer MCF-7 cells due to effective curcumin loading and low particle aggregation . Examples of liposomal delivery systems of CU were Diacyl phosphocholine liposomes and coated or uncoated flexible liposomal systems. Diacyl phosphocholine nanovectors had six molecules of phosphatidylcholine binding one molecule of Curcumin . The drug was inserted into the membrane in a transbilayer orientation, anchored by hydrogen bonding to the phosphate group of phospholipids , increasing drug bioavailability. Similarly, Silica-coated flexible liposomes loaded with curcumin (CUR-SLNs) and curcumin-loaded flexible liposomes (CUR-FLs) without silica-coatings had higher bioavailability compared to that of free curcumin suspensions . Other particularly suitable nanocarriers were Solid-lipid nanoparticles: Tiyaboonchai et al.  used Dioctyl sodium sulfosuccinate and Poloxamer 188 SLNs in a microemulsion technique to produce drug delivery systems sized less than 450 nm and showing up to 70% incorporation efficiency of curcuminoids. The same type of nanovectors were developed for the treatment of Alzheimer’s disease by Kakkar and Kaur , and further improved by Mulik et al. , demonstrating how transferrinmediated solid lipid nanoparticles containing curcumin had significant increases in apoptosis, cytotoxicity, ROS, and cellular uptake compared to a curcumin solubilized surfactant solutions and curcumin-loaded solid lipid nanoparticles, in breast cancer.
Polyphenols are among the most powerful active compounds synthesized by plants and marine organisms, and show a unique combination of chemical, biological and physiological activities. However, their limited stability and/or solubility, often combined with a poor bioavailability, have to be resolved in order to make these compounds able to answer growing demands in human health. In this review, the results of recent studies implementing various delivery techniques applied to polyphenolic compounds confirmed that nanoencapsulation is an interesting means to improve their activity. The various reported research revealed that physicochemical nanoencapsulation provided a significant protection against drastic conditions such as oxidation and thermal degradation, thereby contributing to increase the shelf life of the active ingredients. Furthermore, nanoparticles are also able to control the release, change the physical properties of the initial material, and improve the bioavailability of the polyphenolic compound. Future developments must be aimed to complete the characterization of absorption, distribution, metabolism and elimination behavior of nanosized systems carrying the polyphenols in order to potentiate their therapeutic applications.
We thank Dr. Orsolina Petillo for editing the manuscript and for administrative assistance. This work was supported by Progetto PON—“Ricerca e Competitività 2007–2013”—PON01_01802: “Sviluppo di molecole capaci di modulare vie metaboliche intracellulari redox-sensibili per la prevenzione e la cura di patologie infettive, tumorali, neurodegenerative e loro delivery mediante piattaforme nano tecnologiche”, PON01_02512: “Ricerca e sviluppo di bioregolatori attivi sui meccanismi epigenetici dei processi infiammatori nelle malattie croniche e degenerative”, PON03_00106: “Materiali Avanzati per la Ricerca ed il comparto Agroalimentare, Laboratorio Pubblico-Privato, MAReA” and PRIN 2012 (prot. 201288JKYY): “Nanotecnologie per variare i programmi di sviluppo osseo nella parete vasale per la prevenzione e trattamento delle patologie associate alla calcificazione ectopica arteriosa”.
- Beckman C (2000) Phenolic-storing cells: Keys to programmed cell death and periderm formation in wilt disease resistance and in general defense responses in plants? Physiological and Molecular Plant Pathology 57: 101-110.
- Klejdus B, LojkovÃ¡ L, Plaza M, SnÃ³blovÃ¡ M, StÄ›rbovÃ¡ D (2010) Hyphenated technique for the extraction and determination of isoflavones in algae: ultrasound-assisted supercritical fluid extraction followed by fast chromatography with tandem mass spectrometry. J Chromatogr A 1217: 7956-7965.
- Liu RH (2003) Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am J ClinNutr 78: 517S-520S.
- Pandey KB, Rizvi SI (2009) Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev 2: 270-278.
- McManus JP, Davis KG, Beart JE, Gaffney SH, Lilley TH, et al. (1985) Polyphenol interactions. Part 1.Introduction; some observations on the reversible complexation of polyphenols with proteins and polysaccharides. J. Chem. Soc Perkin 2: 1429–1438.
- Perron NR, Brumaghim JL (2009) A review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell BiochemBiophys 53: 75-100.
- Kim HS, Quon MJ, Kim JA3 (2014) New insights into the mechanisms of polyphenols beyond antioxidant properties; lessons from the green tea polyphenol, epigallocatechin 3-gallate. Redox Biol 2: 187-195.
- Nijveldt RJ, van Nood E, van Hoorn DE, Boelens PG, van Norren K, et al. (2001) Flavonoids: a review of probable mechanisms of action and potential applications. Am J ClinNutr 74: 418-425.
- Fraga CG, Galleano M, Verstraeten SV, Oteiza PI (2010) Basic biochemical mechanisms behind the health benefits of polyphenols. Mol Aspects Med 31: 435-445.
- Scalbert A, Williamson G (2000) Dietary intake and bioavailability of polyphenols. J Nutr 130: 2073S-85S.
- D'Archivio M1, Filesi C, VarÃ¬ R, Scazzocchio B, Masella R (2010) Bioavailability of the polyphenols: status and controversies. Int J MolSci 11: 1321-1342.
- Etheridge ML, Campbell SA, Erdman AG, Christy LH, Susan MW, et al. (2013) The big picture on nanomedicine: the state of investigational and approved nanomedicine products. Nanomedicine: Nanotechnology, Biology, and Medicine 9:1-14.
- Acosta E (2009) Bioavailability of nanoparticles in nutrient and nutraceutical delivery. Current Opinion in Colloid and Interface Science 14: 3-15.
- Nair HB, Sung B, Yadav VR, Kannappan R, Chaturvedi MM, et al. (2010) Delivery of antiinflammatorynutraceuticals by nanoparticles for the prevention and treatment of cancer. BiochemPharmacol 80: 1833-1843.
- Grotewold E (2005) The Science of Flavonoids, Springer, New York, NY, pp:274.
- Milbury PE1, Vita JA, Blumberg JB (2010) Anthocyanins are bioavailable in humans following an acute dose of cranberry juice. J Nutr 140: 1099-1104.
- Bermudez-Soto M, Tomas-Barberan F, Garcia-Conesa M (2007) Stability of polyphenols in chokeberry (Aroniamelanocarpa) subjected to in vitro gastric and pancreatic digestion. Food Chemistry 102: 865–874.
- Ader P, Wessmann A, Wolffram S (2000) Bioavailability and metabolism of the flavonol quercetin in the pig. Free Radic Biol Med 28: 1056-1067.
- Brand W, Padilla B, van Bladeren PJ, Williamson G, Rietjens IM (2010) The effect of co-administered flavonoids on the metabolism of hesperetin and the disposition of its metabolites in Caco-2 cell monolayers. MolNutr Food Res 54: 851-860.
- Lesser S, Cermak R, Wolffram S (2004) Bioavailability of quercetin in pigs is influenced by the dietary fat content. J Nutr 134: 1508-1511.
- Lam W, Kazi A, Kuhn D, Chow LMC, Chan ASC, et al. (2004) A potential pro-drug for a green tea polyphenol proteasome inhibitor: evaluation of the peracetate ester of (-)epigallocatechingallate [(-)-EGCG]. Bioorganic and Medicinal Chemistry 12: 5587–5593.
- Musthaba SM, Baboota S, Ahmed S, Ahuja A, Ali J (2009) Status of novel drug delivery technology for phytotherapeutics. Expert Opin Drug Deliv 6: 625-637.
- Pinto Reis C, Neufeld RJ, Ribeiro AJ, Veiga F (2006) Nanoencapsulation I. Methods for preparation of drug-loaded polymeric nanoparticles. Nanomedicine 2: 8-21.
- Wais U, Jackson AW, He T, Zhang H (2016) Nanoformulation and encapsulation approaches for poorly water-soluble drug nanoparticles. Nanoscale 8: 1746-1769.
- Merisko-Liversidge E, Liversidge GG (2011) Nanosizing for oral and parenteral drug delivery: a perspective on formulating poorly-water soluble compounds using wet media milling technology. Adv Drug Deliv Rev 63: 427-440.
- Groneberg DA, Giersig M, Welte T, Pison U (2006) Nanoparticle-based diagnosis and therapy. Curr Drug Targets 7: 643-648.
- Szejtli J (1998) Introduction and General Overview of Cyclodextrin Chemistry. Chem Rev 98: 1743-1754.
- Eastburn SD, Tao BY (1994) Applications of modified cyclodextrins. BiotechnolAdv 12: 325-339.
- Stella VJ, He Q (2008) Cyclodextrins. ToxicolPathol 36: 30-42.
- Tros de Ilarduya MC, MartÃn C, GoÃ±i MM, MartÃnez-OhÃ¡rriz MC (1998) Solubilization and interaction of sulindac with beta-cyclodextrin in the solid state and in aqueous solution. Drug DevInd Pharm 24: 301-306.
- Singh A, Garg G, Sharma PK (2010) Nanospheres: A Novel Approach For Targeted Drug Delivery System. International Journal of Pharmaceutical Sciences Review and Research 5: 84-88.
- Shive MS, Anderson JM (1997) Biodegradation and biocompatibility of PLA and PLGA microspheres. Adv Drug Deliv Rev 28: 5-24.
- Guterres SS, Alves MP, Pohlmann AR (2007) Polymeric nanoparticles, nanospheres and nanocapsules, for cutaneous applications. Drug Target Insights 2: 147-157.
- Anton N, Benoit JP, Saulnier P (2008) Design and production of nanoparticles formulated from nano-emulsion templates-a review. J Control Release 128: 185-199.
- Khoee S, Yaghoobian M (2009) An investigation into the role of surfactants in controlling particle size of polymeric nanocapsules containing penicillin-G in double emulsion. Eur J Med Chem 44: 2392-2399.
- Radtchenko I, Sukhorukov G, MÃ¶hwald H (2002) A novel method for encapsulation of poorly water-soluble drugs: precipitation in polyelectrolyte multilayer shells. Int J Pharm 242: 219-223.
- Kakkar V, Kaur IP (2011) Evaluating potential of curcumin loaded solid lipid nanoparticles in aluminium induced behavioural, biochemical and histopathological alterations in mice brain. Food ChemToxicol 49: 2906-2913.
- Mulik RS, MÃ¶nkkÃ¶nen J, Juvonen RO, Mahadik KR, Paradkar AR (2010) Transferrin mediated solid lipid nanoparticles containing curcumin: enhanced in vitro anticancer activity by induction of apoptosis. Int J Pharm 398: 190-203.
- Pandita D, Kumar S, Poonia N, Lather V (2014) Solid lipid nanoparticles enhance oral bioavailability of resveratrol, a natural polyphenol. Food Research International 62: 1165-1174.
- Neves AR, LÃºcio M, Martins S, Lima JL, Reis S (2013) Novel resveratrol nanodelivery systems based on lipid nanoparticles to enhance its oral bioavailability. Int J Nanomedicine 8: 177-187.
- Li L, Braiteh FS, Kurzrock R (2005) Liposome-encapsulated curcumin: in vitro and in vivo effects on proliferation, apoptosis, signaling, and angiogenesis. Cancer 104: 1322-1331.
- Li L, Ahmed B, Mehta K, Kurzrock R (2007) Liposomal curcumin with and without oxaliplatin: effects on cell growth, apoptosis and angiogenesis in colorectal cancer. Mol Cancer Ther 6: 1276-1282.
- Pando D, Caddeo C, Manconi M, Fadda AM, Pazos C (2013) Nanodesign of olein vesicles for the topical delivery of the antioxidant resveratrol. J Pharm Pharmacol 65: 1158-1167.
- Chen Y, Zhang X, Lu J, Huang Y, Li J, et al. (2014) Targeted delivery of curcumin to tumors via PEG-derivatized FTS-based micellar system. AAPS J 16: 600-608.
- Amit Singh GG, Sharma PK (2010) Nanospheres: A novel approach for targeted drug delivery system. International Journal of Pharmaceutical Sciences Review and Research 5: 84-88.
- Yow HN, Routh AF (2006) Formation of liquid core–polymer shell microcapsules. Soft Matter 2: 940-949.
- Siddiqui IA, Adhami VM, Bharali DJ, Hafeez BB, Asim M, et al. (2009) Introducing nanochemoprevention as a novel approach forcancer control: Proof of principle with green tea polyphenol epigallocatechin-3-gallate. Cancer Research 69: 1712-1716.
- Zu YG, Yuan S, Zhao XH, Zhang Y, Zhang XN, et al. (2009) [Preparation, activity and targeting ability evaluation in vitro on folate mediated epigallocatechin-3-gallate albumin nanoparticles]. Yao XueXueBao 44: 525-531.
- Sanna V, Pintus G, Roggio AM, Punzoni S, Posadino AM, et al. (2011) Targeted biocompatible nanoparticles for the delivery of (-)-epigallocatechin 3-gallate to prostate cancer cells. J Med Chem 54: 1321-1332.
- Italia JL, Datta P, Ankola DD, Kumar MNVR (2008) Nanoparticles enhance per oral bioavailability of poorly available molecules: Epigallocatechingallate nanoparticles ameliorates cyclosporine induced nephrotoxicity in rats at three times lower dose than oral solution. Journal of biomedical nanotechnology 4: 304–312.
- Rocha S, Generalov R, Pereira MDC, Peres I, Juzenas P, et al. (2011) Epigallocatechingallate-loaded polysaccharide nanoparticles for prostate cancer chemoprevention. Nanomedicine 6: 79–87.
- Smith A, Giunta B, Bickford PC, Fountain M, Tan J, et al. (2010) Nanolipidic particles improve the bioavailability and a-secretase inducing ability of epigallocatechin-3-gallate (EGCG) for the treatment of Alzheimer’s disease. International Journal of Pharmaceutics 389: 207–212.
- Shutava TG, Balkundi SS, Vangala P, Steffan JJ, Bigelow RL, et al. (2009) Layer-by-Layer-Coated Gelatin Nanoparticles as a Vehicle for Delivery of Natural Polyphenols. ACS Nano 3: 1877-1885.
- Dube A, Ng K, Nicolazzo J, Larson I (2010) Effective use of reducing agents and nanoparticle encapsulation in stabilizing catechins in alkaline solution. Food Chemistry 122: 662–667.
- Dube A, Nicolazzo JA, Larson I (2010) Chitosan nanoparticles enhance the intestinal absorption of the green tea catechins (+)-catechin and (-)-epigallocatechingallate. Eur J Pharm Sci 41: 219-225.
- D'Andrea G (2015) Quercetin: A flavonol with multifaceted therapeutic applications? Fitoterapia 106: 256-271.
- Li H1, Zhao X, Ma Y, Zhai G, Li L, et al. (2009) Enhancement of gastrointestinal absorption of quercetin by solid lipid nanoparticles. J Control Release 133: 238-244.
- Barras A, Mezzetti A, Richard A, Lazzaroni S, Roux S, et al. (2009) Formulation and characterization of polyphenol-loaded lipid nanocapsules. Int J Pharm 379: 270-277.
- Wu TH, Yen FL, Lin LT, Tsai TR, Lin CC, et al. (2008) Preparation, physicochemical characterization, and antioxidant effects of quercetin nanoparticles. Int J Pharm 346: 160-168.
- Pool H, Quintanar D, de Dios Figueroa J (2012) Antioxidant effects of quercetin and catechin encapsulated into PLGA nanoparticles. Journal of Nanomaterials 1-12.
- Ghosh A, Mandal AK, Sarkar S, Panda S, Das N (2009) Nanoencapsulation of quercetin enhances its dietary efficacy in combating arsenic-induced oxidative damage in liver and brain of rats. Life Sci 84: 75-80.
- Ghosh S, Dungdung SR, Chowdhury ST, Mandal AK, Sarkar S, et al. (2011) Encapsulation of the flavonoid quercetin with an arsenic chelator into nanocapsules enables the simultaneous delivery of hydrophobic and hydrophilic drugs with a synergistic effect against chronic arsenic accumulation and oxidative stress. Free Radic Biol Med 51: 1893-1902.
- Chakraborty S, Stalin S, Das N, Choudhury ST, Ghosh S, et al. (2012) The use of nano-quercetin to arrest mitochondrial damage and MMP-9 upregulation during prevention of gastric inflammation induced by ethanol in rat. Biomaterials 33: 2991-3001.
- Kumari A, Yadav SK, Pakade YB, Singh B, Yadav SC (2010) Development of biodegradable nanoparticles for delivery of quercetin. Colloids Surf B Biointerfaces 80: 184-192.
- Mercader-Ros MT, Lucas-Abellán C, Fortea MI, Gabaldón JA, Núñez-Delicado E (2010) Effect of HP-ß-cyclodextrinscomplexation on the antioxidant activity of flavonols. Food Chemistry 118: 769-773.
- Goswami SK, Das DK (2009) Resveratrol and chemoprevention. Cancer Lett 284: 1-6.
- de la Lastra CA, Villegas I (2007) Resveratrol as an antioxidant and pro-oxidant agent: mechanisms and clinical implications. BiochemSoc Trans 35: 1156-1160.
- de la Lastra CA, Villegas I (2005) Resveratrol as an anti-inflammatory and anti-aging agent: mechanisms and clinical implications. MolNutr Food Res 49: 405-430.
- Ulrich S, Wolter F, Stein JM (2005) Molecular mechanisms of the chemopreventive effects of resveratrol and its analogs in carcinogenesis. MolNutr Food Res 49: 452-461.
- Joe AK, Liu H, Suzui M, Vural ME, Xiao D, et al. (2002) Resveratrol induces growth inhibition, S-phase arrest, apoptosis, and changes in biomarker expression in several human cancer cell lines. Clin Cancer Res 8: 893-903.
- Bastianetto S, Zheng WH, Quirion R (2000) Neuroprotective abilities of resveratrol and other red wine constituents against nitric oxide-related toxicity in cultured hippocampal neurons. Br J Pharmacol 131: 711-720.
- Bradamante S, Barenghi L, Villa A (2004) Cardiovascular protective effects of resveratrol. Cardiovasc Drug Rev 22: 169-188.
- Baur JA, Pearson KJ, Price NL, Jamieson HA, Lerin C, et al. (2006) Resveratrol improves health and survival of mice on a high-calorie diet. Nature 444: 337-342.
- Agustin-Salazar S, Gamez-Meza N, Medina-Jua`rez LA, Soto-Valdez H, Cerruti P (2014) From nutraceutics to materials: effect of resveratrol on the stability of polylactide. ACS Sustainable Chemistry and Engineering 2: 1534–1542.
- Walle T, Hsieh F, DeLegge MH, Oatis JE Jr, Walle UK (2004) High absorption but very low bioavailability of oral resveratrol in humans. Drug MetabDispos 32: 1377-1382.
- Vitaglione P, Sforza S, Galaverna G, Ghidini C, Caporaso N, et al. (2005) Bioavailability of trans-resveratrol from red wine in humans. MolNutr Food Res 49: 495-504.
- Caddeo C, Teskac K, Sinico C, Kristl J (2008) Effect of resveratrol incorporated in liposomes on proliferation and UV-B protection of cells. Int J Pharm 363: 183-191.
- Junfei Shao XL, Lu X, Jiang C, Hu Y, Li Q, et al. (2009) Enhanced growth inhibition effect of Resveratrol incorporated into biodegradable nanoparticles against glioma cells is mediated by the induction of intracellular reactive oxygen species levels. Colloids and Surfaces B: Biointerfaces 72: 40-47.
- Singh G, Pai RS (2014) Optimized PLGA nanoparticle platform for orally dosed trans-resveratrol with enhanced bioavailability potential. Expert Opin Drug Deliv 11: 647-659.
- Singh G, Pai RS (2014) In-vitro/in-vivo characterization of trans-resveratrol-loaded nanoparticulate drug delivery system for oral administration. J Pharm Pharmacol 66: 1062-1076.
- Zu Y, Zhang Y, Wang W, Zhao X, Han X, et al. (2016) Preparation and in vitro/in vivo evaluation of resveratrol-loaded carboxymethyl chitosan nanoparticles. Drug Deliv 23: 981-991.
- Teskac K, Kristl J (2010) The evidence for solid lipid nanoparticles mediated cell uptake of resveratrol. Int J Pharm 390: 61-69.
- Jose S, Anju SS, Cinu TA, Aleykutty NA, Thomas S, et al. (2014) In vivo pharmacokinetics and biodistribution of resveratrol-loaded solid lipid nanoparticles for brain delivery. Int J Pharm 474: 6-13.
- Coimbra M, Isacchi B, van Bloois L, Torano JS, Ket A, et al. (2011) Improving solubility and chemical stability of natural compounds for medicinal use by incorporation into liposomes. Int J Pharm 416: 433-442.
- Basavaraj S, Betageri GV (2014) Improved oral delivery of resveratrol using proliposomal formulation: investigation of various factors contributing to prolonged absorption of unmetabolized resveratrol. Expert Opin Drug Deliv 11: 493-503.
- Lu X, Ji C, Xu H, Li X, Ding H, et al. (2009) Resveratrol-loaded polymeric micelles protect cells from Abeta-induced oxidative stress. Int J Pharm 375: 89-96.
- Ansari KA, Vavia PR, Trotta F, Cavalli R (2011) Cyclodextrin-based nanosponges for delivery of resveratrol: in vitro characterisation, stability, cytotoxicity and permeation study. AAPS PharmSciTech 12: 279-286.
- Aviram M, Rosenblat M, Gaitini D, Nitecki S, Hoffman A, et al. (2004) Pomegranate juice consumption for 3 years by patients with carotid artery stenosis reduces common carotid intima-media thickness, blood pressure and LDL oxidation. ClinNutr 23: 423-433.
- Esmaillzadeh A, Tahbaz F, Gaieni I, Alavi-Majd H, Azadbakht L (2004) Concentrated pomegranate juice improves lipid profiles in diabetic patients with hyperlipidemia. J Med Food 7: 305-308.
- Aviram M, Dornfeld L (2001) Pomegranate juice consumption inhibits serum angiotensin converting enzyme activity and reduces systolic blood pressure. Atherosclerosis 158: 195-198.
- Kulkarni AP, Mahal HS, Kapoor S, Aradhya SM (2007) In vitro studies on the binding, antioxidant and cytotoxic actions of punicalagin. J Agric Food Chem 55: 1491-1500.
- Mertens-Talcott SU, Jilma-Stohlawetz P, Rios J, Hingorani L, Derendorf H (2006) Absorption, metabolism, and antioxidant effects of pomegranate (Punicagranatuml.) polyphenols after ingestion of a standardized extract in healthy human volunteers. J Agric Food Chem 54: 8956-8961.
- Kim ND, Mehta R, Yu W, Neeman I, Livney T, et al. (2002) Chemopreventive and adjuvant therapeutic potential of pomegranate (Punicagranatum) for human breast cancer. Breast Cancer Res Treat 71: 203-217.
- Albrecht M, Jiang W, Kumi-Diaka J, Lansky EP, Gommersall LM, et al. (2004) Pomegranate extracts potently suppress proliferation, xenograft growth, and invasion of human prostate cancer cells. J Med Food 7: 274-283.
- Shibata T, Ishimaru K, Kawaguchi S, Yoshikawa H, Hama Y (2008) Antioxidant activities of phlorotannins isolated from Japanese Laminariaceae. Journal of Applied Phycology 20: 705-711.
- Bala I, Bhardwaj V, Hariharan S, Kumar MN (2006) Analytical methods for assay of ellagic acid and its solubility studies. J Pharm Biomed Anal 40: 206-210.
- Lei F, Xing DM, Xiang L, Zhao YN, Wang W, et al. (2003) Pharmacokinetic study of ellagic acid in rat after oral administration of pomegranate leaf extract. J Chromatogr B AnalytTechnol Biomed Life Sci 796: 189-194.
- Li Z, Percival SS, Bonard S, Gu L (2011) Fabrication of nanoparticles using partially purified pomegranate ellagitannins and gelatin and their apoptotic effects. MolNutr Food Res 55: 1096-1103.
- Arulmozhi V, Pandian K, Mirunalini S (2013) Ellagic acid encapsulated chitosan nanoparticles for drug delivery system in human oral cancer cell line (KB). Colloids Surf B Biointerfaces 110: 313-320.
- Bala I, Bhardwaj V, Hariharan S, Kharade SV, Roy N, et al. (2006) Sustained release nanoparticulate formulation containing antioxidant-ellagic acid as potential prophylaxis system for oral administration. J Drug Target 14: 27-34.
- Sonaje K, Italia JL, Sharma G, Bhardwaj V, Tikoo K, et al. (2007) Development of biodegradable nanoparticles for oral delivery of ellagic acid and evaluation of their antioxidant efficacy against cyclosporine A-induced nephrotoxicity in rats. Pharm Res 24: 899-908.
- Shirode AB, Bharali DJ, Nallanthighal S, Coon JK, Mousa SA, et al. (2015) Nanoencapsulation of pomegranate bioactive compounds for breast cancer chemoprevention. Int J Nanomedicine 10: 475-484.
- D'Andria R, Di Salle A, Petillo O, Sorrentino G, Peluso G (2013) Nutraceutical, cosmetic, health products derived from olive. Present and future of the Mediterranean olive sector. Options méditerranéennes SERIES A: Mediterranean Seminars, CIHEAM.
- Saija A, Trombetta D, Tomaino A, Lo Cascio R, Princi P, et al. (1998) In vitro evaluation of the antioxidant activity and biomembrane interaction of the plant phenols oleuropein and hydroxytyrosol. International Journal of Pharmaceutics 166: 123-133.
- Haghighi SM, Ebrahimpourmoghaddam S, Purkhosrow A, Mohammadi S, Nekooein AA, et al. (2012) Magnetic Bovine Serum Albumin-based nanoparticles as potential controlled release drug delivery systems. Proceedings of the 4thInternational Conference on Nanostructures (ICNS4), Kish Island, I.R. Iran.
- Hussain Z, Katas H, Amin MCI, Kumolosasi E, Buang F, et al. (2013) Self-assembled polymeric nanoparticles for percutaneous co-delivery of hydrocortisone/hydroxytyrosol: An ex vivo and in vivo study using an NC/Nga mouse model. International Journal of Pharmaceutics 444: 109–119.
- Katas H, Amin MCI, Sahudin S, Buang F (2013) Chitosan-based skin-targeted nanoparticle drug delivery system and method.
- Siddique MI, Katas S, Amin MCI, Ng SF, Zulfakar MH, et al. (2015) Minimization of Local and Systemic Adverse Effects of Topical Glucocorticoids by Nanoencapsulation: In Vivo Safety of Hydrocortisone–Hydroxytyrosol Loaded Chitosan Nanoparticles. Journal of Pharmaceutical Sciences 104: 4276-4286.
- Guan Q, Sun S, Li X, Lv S, Xu T, et al. (2016) Preparation, in vitro and in vivo evaluation of mPEG-PLGA nanoparticles co-loaded with syringopicroside and hydroxytyrosol. J Mater Sci Mater Med 27: 24.
- Mourtzinos I, Salta F, Yannakopoulou K, Chiou A, Karathanos VT (2007) Encapsulation of olive leaf extract in beta-cyclodextrin. J Agric Food Chem 55: 8088-8094.
- López-García MA, López O, Maya I, Fernández-Bolaños JG (2010) Complexation of hydroxytyrosol with ß-cyclodextrins. An efficient photoprotection. Tetrahedron 66: 8006–8011.
- Mohammadi A, Jafari SM, Assadpour E, FaridiEsfanjani A (2016) Nano-encapsulation of olive leaf phenolic compounds through WPC-pectin complexes and evaluating their release rate. Int J BiolMacromol 82: 816-822.
- Yadav VR, Aggarwal BB (2011) Curcumin: a component of the golden spice, targets multiple angiogenic pathways. Cancer BiolTher 11: 236-241.
- Rasyid A, Rahman AR, Jaalam K, Lelo A (2002) Effect of different curcumin dosages on human gall bladder. Asia Pac J ClinNutr 11: 314-318.
- Menon VP, Sudheer AR (2007) Antioxidant and anti-inflammatory properties of curcumin. AdvExp Med Biol 595: 105-125.
- Bhattacharyya S, Mandal D, Sen GS, Pal S, Banerjee S, et al. (2007) Tumor-induced oxidative stress perturbs nuclear factor-kappaB activity-augmenting tumor necrosis factor-alpha-mediated T-cell death: protection by curcumin. Cancer Res 67: 362-370.
- Jurenka JS (2009) Anti-inflammatory properties of curcumin, a major constituent of Curcuma longa: a review of preclinical and clinical research. Alternative Medicine Review 14: 141-153.
- Anand P, Kunnumakkara AB, Newman RA, Aggarwal BB (2007) Bioavailability of curcumin: problems and promises. Mol Pharm 4: 807-818.
- Kazemi-Lomedasht F, Rami A, Zarghami N (2013) Comparison of inhibitory effect of curcumin nanoparticles and free curcumin in human telomerase reverse transcriptase gene expression in breast cancer. Adv Pharm Bull 3: 127-130.
- Pulla Reddy AC, Sudharshan E, AppuRao AG, Lokesh BR (1999) Interaction of curcumin with human serum albumin--a spectroscopic study. Lipids 34: 1025-1029.
- Kim TH, Jiang HH, Youn YS, Park CW, Tak KK, et al. (2011) Preparation and characterization of water-soluble albumin-bound curcumin nanoparticles with improved antitumor activity. Int J Pharm 403: 285-291.
- Sahu A, Kasoju N, Goswami P, Bora U (2011) Encapsulation of curcumin in Pluronic block copolymer micelles for drug delivery applications. J BiomaterAppl 25: 619-639.
- Alizadeh AM, Sadeghizadeh M, Najafi F, Ardestani SK, Erfani-Moghadam V, et al. (2015) Encapsulation of curcumin in diblock copolymer micelles for cancer therapy. Biomed Res Int 2015: 824746.
- Mayol L, Serri C, Menale C, Crispi S, Piccolo MT, et al. (2015) Curcumin loaded PLGA-poloxamer blend nanoparticles induce cell cycle arrest in mesothelioma cells. Eur J Pharm Biopharm 93: 37-45.
- Subramanian SB, Francis AP, Devasena T (2014) Chitosan-starch nanocomposite particles as a drug carrier for the delivery of bis-desmethoxycurcuminanalog. CarbohydrPolym 114: 170-178.
- Verderio P, Bonetti P, Colombo M, Pandolfi L, Prosperi D (2013) Intracellular drug release from curcumin-loaded PLGA nanoparticles induces G2/M block in breast cancer cells. Biomacromolecules 14: 672-682.
- Goethals EC, Shukla R, Mistry V, Bhargava SK, Bansal V (2013) Role of the templating approach in influencing the suitability of polymeric nanocapsules for drug delivery: LbLvs. SC/MS. Langmuir. 29: 12212–12219.
- Began G, Sudharshan E, UdayaSankar K, AppuRao AG (1999) Interaction of curcumin with phosphatidylcholine: A spectrofluorometric study. Journal of agricultural and food chemistry 47: 4992-4997.
- Barry J, Fritz M, Brender JR, Smith PE, Lee DK, et al. (2009) Determining the effects of lipophilic drugs on membrane structure by solid-state NMR spectroscopy: the case of the antioxidant curcumin. Journal of the American Chemical Society 131: 4490-4498.
- Li C, Zhang Y, Su T, Feng L, Long Y, et al. (2012) Silica-coated flexible liposomes as a nanohybrid delivery system for enhanced oral bioavailability of curcumin. International Journal of Nanomedicine 7: 5995-6002.
- Tiyaboonchai W, Tungpradit W, Plianbangchang P (2007) Formulation and characterization of curcuminoids loaded solid lipid nanoparticles. International Journal of Pharmaceutics 337: 299-306.
Citation: Conte R, Calarco A, Napoletano A, Valentino A, Margarucci S, et al.(2016) Polyphenols Nanoencapsulation for Therapeutic Applications. J Biomol Res Ther 5: 139. Doi: 10.4172/2167-7956.1000139
Copyright: © 2016 Conte R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Select your language of interest to view the total content in your interested language
Share This Article
- Total views: 10304
- [From(publication date): 6-2016 - Oct 23, 2018]
- Breakdown by view type
- HTML page views: 9915
- PDF downloads: 389