alexa Possible Role of AMPK/SIRT1 Signaling on Energy Balance in Geniposide- Treated INS-1 Cells | Open Access Journals
ISSN: 2161-0444
Medicinal Chemistry
Like us on:
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Possible Role of AMPK/SIRT1 Signaling on Energy Balance in Geniposide- Treated INS-1 Cells

Lixia Guo1, Chunyan Liu2, Fei Yin1,2 and Jianhui Liu1,2*

1Chongqing Key Lab of Catalysis and Functional Organic Molecules, Chongqing Technology and Business University, Chongqing, PR China

2College of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, PR China

*Corresponding Author:
Prof. Jianhui Liu
College of Pharmacy and Bioengineering
Chongqing University of Technology
Chongqing, 400054, PR China
Tel: 862362563190
E-mail: [email protected]

Received date: January 11, 2016; Accepted date: January 20, 2016; Published date: January 25, 2016

Citation: Guo L, Liu C, Yin F, Liu J (2016) Possible Role of AMPK/SIRT1 Signaling on Energy Balance in Geniposide-Treated INS-1 Cells. Med chem 6:033-038. doi:10.4172/2161-0444.1000319

Copyright: © 2016 Guo L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Medicinal Chemistry

Abstract

Our previous work showed that in pancreatic INS-1 cells, geniposide exerted a contrary role on both glucosestimulated insulin secretion (GSIS) and glucose uptake and metabolism in the presence of low and high glucose. But the molecular mechanisms are presently not well understood. In the present study, we design to probe the role of AMP-activated protein kinase (AMPK) and NAD+- dependent deacetylase sirtuin-1 (SIRT1) on geniposide regulating GSIS, and analyze the interaction between AMPK and SIRT1 in pancreatic β cells. Our results indicate that geniposide induce the phosphorylation of AMPK and enhance the expression of SIRT1 in the presence of low concentration of glucose, but in the presence of high concentrations of glucose, geniposide played a contrary role on those. Furthermore, Compound C (an AMPK inhibitor) and Ex527 (a potent and selective inhibitor of SIRT1) prevent the effects of geniposide on glucose uptake, ATP production and GSIS in INS-1 cells. Taken together, our findings suggest that AMPK/SIRT1 are associated with the role of geniposide on energy balance in INS-1 cells.

Keywords

AMP-activated protein kinase (AMPK); Geniposide; Glucose stimulating insulin secretion (GSIS); NAD+- dependent deacetylase sirtuin-1 (SIRT1)

Introduction

AMP-activated protein kinase (AMPK), known as a “metabolic master switch”, is one of the essential players in cellular energy regulation adapting cellular demands to nutritional and metabolic variations [1]. Usually, in pancreatic β cells, nutrient uptake and glucose metabolism increases the ratio of ATP/ADP leading to closure of the K+/ATP channel. Accumulation of K+ inside the cell leads to membrane depolarization that in turn opens voltage-sensitive calcium channels and calcium ion influx. Calcium triggers exocytosis of the readily releasable pool of insulin granules docked at the plasma membrane resulting insulin secretion [2]. In this process, AMPK may control several of these events to regulate insulin secretion, in especial including glucose metabolism. Activation of AMPK results in the inhibition of energyconsuming pathways and activation of ATP-producing pathways [3,4]. So, pharmacological stimulation of the enzyme exerts pleiotropic beneficial effects on metabolic homeostasis including muscle glucose uptake, fatty acid oxidation, decrease of hepatic gluconeogenesis, inhibition of lipolysis and lowering of triglyceride concentration [5].

Furthermore, the increasing data shows that AMPK and s NAD+- dependent deacetylase sirtuin-1 (SIRT1) can activate each other, which raises the possibility that they are components of a cycle [6-8]. SIRT1 is widely expressed in mammalian tissues [9,10], including pancreas (β cells), liver, skeletal muscle, adipose tissue, brain, and endothelium, and it is an NAD+-dependent histone/protein deacetylase whose activity is also regulated by nutrient. A change in energy state or activation of AMPK leads to activation of SIRT1, perhaps by increasing NAD+ or the NAD/NADH ratio [7] and/or the activity of Nampt [8]. SIRT1 then deacetylates and activates LKB1, which in turn activates AMPK. Glucose metabolism and calorie regulation by AMPK/SIRT1 sensing network have been shown to improve insulin sensitivity [2], decrease ectopic lipid deposition [11,12], and prevent pancreatic β-cell damage [13]. So, AMPK/SIRT1 have emerged as an new target to treat metabolic diseases such as type 2 diabetes and obesity [14,15].

Our previous studies have shown that geniposide enhanced glucose stimulated insulin secretion (GSIS) in the presence of low or moderately high concentrations of glucose in pancreatic β cells [16,17]. However, geniposide exerted an acute inhibitory effect on GSIS when the cells were challenged with a high concentration of glucose (over 25 mM). We also observed that, the effects of geniposide on GSIS were associated with glucose uptake and metabolism by regulating the expression of pyruvate carboxylase [17]. These are intriguing results suggest a potential role of geniposide in regulating GSIS, at least in part, was a result of glucose metabolism, which was a key process for insulin release. However, the mechanisms of geniposide regulating glucose uptake and metabolism in INS-1 cells need to be further investigated.

Mounting evidence shows that AMPK/SIRT1 are the key players of glucose metabolism [18-20]. In the present study, beside to probe the role of AMPK/ SIRT1 on geniposide regulating GSIS in pancreatic β cells, Compound C, a specific AMPK inhibitor, and Ex 527, a specific SIRT1 inhibitor, were used to explore the role of AMPK and SIRT1 on geniposide regulating GSIS in pancreatic β cells.

Materials and Methods

Materials

“Compound C” (6-[4-(2-piperidin-1-yletoxy)-phenyl)]-3- pyridin-4–yl–pyrrazolo [1,5-a]pyrimidine), an AMPK inhibitor, and Ex 527, a potent and selective SIRT1 inhibitor, were purchased from Sigma-Aldrich (St Louis, MO, USA). Rat/Mouse insulin ELISA kit was obtained from Millipore Corporation (Billerica, MA, USA). Specific antibodies against phospho-AMPK, AMPKα1/2, SIRT1, and antimouse and anti-rabbit HRP-conjugated antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

Cell culture

INS-1 cells, a rat pancreatic β-cell line, was purchased from CCTCC (China Center for Type Culture Collection). The cells were cultured in RPMI medium 1640 with 11 mM glucose and supplemented with 10% fetal bovine serum (FBS), 10 mM HEPES, 100 U/ml penicillin, 100 μg/ ml streptomycin, 2 mM Lglutamine, 1 mM sodium pyruvate and 50 μM mercaptoethanol under 5% CO2 and 95% air at 37°C.

Assay of glucose uptake and ATP level

To determine the effect of geniposide on glucose uptake and metabolism, INS-1 cells were seeded onto 6-well plates. After overnight incubation, the cells were washed once with KRBB and starved for 2 hours in KRBH buffer. And then, an AMPK inhibitor Compound C, or a SIRT1 inhibitor Ex 527 were added respectively in the presence or absence of 10 μM geniposide with KRBB containing 5.5 or 33 mM of glucose. After incubated for 20 minutes, the buffer was collected for the measurement of glucose concentration, which was used to calculate glucose uptake as reported previously 17. The cell lysates were used to determine ATP content. Glucose concentration in the buffer was measured using a glucose assay kit according to protocol supplied by the manufacturer (Bioversion, Mountain View, CA). The content of ATP in cell lysates was measured using ATP bioluminescence assay kits according to the manufacturer’s instructions (Roche, Mannheim, Germany).

Immunoblotting

After treated with geniposide in the presence of different concentrations of glucose, INS-1 cells were washed with cold PBS and lysed in a lysis buffer containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 0.1% sodium dodecyl sulfate, protease inhibitors (aprotinin, 30 μg/ml; leupeptin, 4 μg/ml; pepstatin, 2 μg/ml; and phenylmethyl sulfonyl fluoride, 10 μg/ml), 1 mM Na3VO4, and 2.5 mM Na4P2O7. Lysates were sonicated for approximately 10 s, protein concentrations were determined by a BCA protein assay kit (Beyotime, Shanghai, China). The samples were stored at -80°C until use. An aliquot of 10-20 μg protein from each cell extract was loaded on a 10% SDS-PAGE gel. After electrophoretic separation, proteins were transferred to polyvinylidene difluoride (PVDF) membrane. Primary and secondary antibodies were diluted in a blocking solution and incubated with the membrane for indicated times as described previously [21]. Excess antibody was washed off with 20 mM Tris-buffered saline containing Tween-20 (TBST, 20 mM Tris, 150 mM NaCl and 0.1% Tween 20; pH 7.5). Immunoreactivity was detected using enhanced chemiluminescence (ECL) western blotting kit (Amersham Pharmacia Biotech AB, Uppsala, Sweden). Bands were analyzed by densitometric scanning using the Quantity One software (Bio-Rad, Hercules, CA, USA).

Statistical analysis

All statistical analyses were conducted using the software of Origin version 8.0 (OriginLab Corporation, MA). Data were analyzed using one-way ANOVA followed with the Tukey’s post-doc test or twoway ANOVA followed with the Bonferroni’s post-hoc test for the differences among the treatment means, where P<0.05 was considered significant. Results are presented as means ± SD from at least three times experiments.

Results

Effects of geniposide on the phosphorylation of AMPK and the expression of SIRT1

In this study, we determined the effect of geniposide on the phosphorylations of AMPK and the expression of SIRT1 in the presence of low (5.5 mM) and high (33 mM) concentrations of glucose. As shown in Figure 1A, comparing with the control at 30-minute time point, 10 μM geniposide treatment significantly increased the phosphorylated level of AMPK protein to 3.39 ± 0.42 folds (p<0.01) in the presence of 5.5 mM glucose. But in the presence of 33 mM glucose, after a shortterm increasing, geniposide suppressed the phosphorylation of AMPK distinctively. As shown in Figure 2B, comparing with the control at 60-minute time point, the phosphorylated level of AMPk decreased to 42% in geniposide-treated INS-1 cells (p<0.01).

medicinal-chemistry-geniposide-phosphorylation-AMPK

Figure 1: Effects of geniposide on the phosphorylation of AMPK in presence of 5.5 (A) or 33 (B) mM glucose in INS-1 cells. After INS-1 cells were treated with 10 μM geniposide for indicated times, the cells were washed once with PBS, and equal lysates were separated with SDS-PAGE. The phosphorylation of AMPK was probed with western blotting. Data are shown as mean ± SD from three independent experiments. *P<0.05, **P<0.01 vs vehicle.

medicinal-chemistry-Geniposide-concentration-glucose

Figure 2: Geniposide regulated the expression of SIRT1 in the presence of low and high concentration of glucose in INS-1 cells. A: Treatment with 10 μM geniposide increases the protein levels of SIRT1 in response to 5.5 mM of glucose in INS-1 cells, **P<0.01 vs vehicle. B: Treatment with 10 μM geniposide decreases the protein levels of SIRT1 in response to 33 mM of glucose in INS-1 cells, **P<0.01 vs vehicle. Data are means ± SD from at least three independent experiments.

Similarly, the level of SIRT1 was significantly increased to 2.32 ± 0.25 folds (p<0.01) in cells treated with 10 μM geniposide comparing with the control (Figure 2A). But in the presence of 33 mM glucose, geniposide dramatically inhibited the expression of SIRT1. As shown in Figure 2B, comparing with the control at 24-hour time point, treatment with 10 μM geniposide decreased the protein level of SIRT1 to 40% (p<0.01).

Interaction of AMPK and SIRT1 in geniposide-treating INS- 1 cells

To explore the interaction of AMPK and SIRT1, we firstly determined the effect of Compound C (an AMPK inhibitor) on the expression of SIRT1, as shown in Figure 3, we observed that, after treatment with 10 μM geniposide for 24 hours, the protein level of SIRT1 was increased to 1.68 ± 0.21 folds in the presence of 5.5 mM glucose (p<0.05), but in the presence of 33 mM glucose, the expression level of SIRT1 was inhibited to about 62% compared to the control. Furthermore, preincubation with 50 μM Compound C, an AMPK inhibitor, decreased the expression of SIRT1 induced by geniposide both in the presence of 5.5 and 33 mM glucose in INS-1 cells.

medicinal-chemistry-glucose-inhibitor-geniposide

Figure 3: Effects of Compound C on the expression of SIRT1 in presence of 5.5 (A) or 33 (B) mM glucose in INS-1 cells. After INS-1 cells were treated with 50 μM Compound C, a specific inhibitor for AMPK for 30 min, 10 μM geniposide was added into the INS-1 cells and continued to culture for 24 h. After that, the cells were washed once with PBS, and equal lysates were separated with SDS-PAGE. The expression of SIRT1 was probed with western blotting. Data are shown as mean ± SD from three independent experiments. **P<0.01 vs control, and ##P<0.01 vs the group of geniposide alone.

At the same time, we also measured the influence of Ex 527, a potent and selective inhibitor of SIRT1, on the phosphorylation of AMPK, the results suggested that treatment with Ex 527 significantly inhibited the phosphorylation of AMPK induced by geniposide both in the presence of 5.5 and 33 mM glucose in INS-1 cells (Figures 4A and 4B).

medicinal-chemistry-phosphorylation-glucose-inhibitor

Figure 4: Effects of Ex 527 on the phosphorylation of AMPK in presence of 5.5 (A) or 33 (B) mM glucose in INS-1 cells. After INS-1 cells were treated with 1 μM Ex 527, a specific inhibitor for SIRT1 for 30 min, 10 μM geniposide was added into the INS-1 cells and continued to culture for 60 min. After that, the cells were washed once with PBS, and equal lysates were separated with SDS-PAGE. The phosphorylation of AMPK was probed with western blotting. Data are shown as mean ± SD from three independent experiments. *P<0.05, **P<0.01 vs control, and ##P<0.01 vs the group of geniposide alone.

Effect of AMPK/SIRT1 on geniposide regulating glucose uptake and intracellular ATP level

Mounting evidence show that glucose uptake and ATP content play a critical role on GSIS [1]. Our previous data suggested that geniposide regulating GSIS were associated with its role on glucose uptake and metabolism [17]. To further probe the mechanisms of geniposide regulating GSIS, we determined the influence of Compound C and Ex 527 on the uptake and metabolism of glucose in geniposide-treating INS-1 cells, the results revealed that, similar with our previous work, geniposide accerlated the uptake and metabolism of glucose in the presence of 5.5 mM glucose, but exerted a contrary role on that in the presence of 33 mM glucose. Furthermore, both Compound C and Ex 527 could suppress the effect of geniposide on glucose uptake (Figure 5A) and the production of ATP (Figure 5B) induced by geniposide both in the presence of 5.5 and 33 mM glucose in INS-1 cells.

medicinal-chemistry-geniposide-regulating-glucose

Figure 5: Effects of AMPK/ SIRT1 on geniposide regulating glucose uptake (A) and ATP level (B). INS-1 cells were seeded onto 6-well plate. After overnight incubation, the cells were washed once with KRBB and starved for 2 hours in KRBH buffer. Then, Compound C or Ex 527 incubated for 30 min respectively before starved for 20 minutes in the presence or absence of 10 μM geniposide with KRBB containing 5.5 or 33 mM of glucose. After 20 minutes of incubation, glucose concentration in the buffer was measured and the uptake of glucose was determined by the difference of glucose concentrations in the buffer after incubation relative to pre-incubation. The intracellular content of ATP was measure in cell lysates using ATP bioluminescence assay kits according to the manufacturer’s instructions. Data are means ± SD from three representative experiments (n=3, three wells for each replicate). *P<0.05, **P<0.01 vs the same glucose concentrations without geniposide. &P<0.05, &&P<0.01 vs the same glucose concentrations with geniposide.

Discussion

Hyperinsulinemia, which is a condition in which there are excess levels of insulin circulating in the blood than expected relative to the level of glucose, is associated with hypertension, obesity, dyslipidemia, and glucose intolerance, and often seen in people with early stage type 2 diabetes mellitus [22]. Recently Lamontagne et al. reported troglitazone exerted a direct action on β cell to reduce insulin secretion in vitro as pioglitazone [23]. At present, it has been popularly Accepted that hyperinsulinemia can cause over insulin secretion and insulin resistance and that lowering insulin secretion in hyperinsulinemic individuals may be beneficial [24].

In our previous study, we reported that geniposide exerted a contrary role on GSIS in the presence of low and very high (over 25 mM) concentrations of glucose by regulating the level of pyruvate carboxylase, a critical enzyme associated with the metabolism of glucose [16,17,25], which suggests that geniposide might be a promising agent to prevent or delay hyperinsulinemia or glucotoxicity under high glucose burden. But unfortunately, the mechanisms of geniposide regulating the uptake and metabolism of glucose need to be further clarified.

AMPK is a major cellular energy sensor and a master regulator of metabolic homeostasis in eukaryotes that maintains energy stores and enhances aerobic metabolism [7]. A large number of physiological processes have been shown to stimulate AMPK, including conditions that lead to alterations of the ATP/AMP ratio and calcium concentrations, which regulates lipid and glucose metabolism through direct phosphorylation of its substrates [5]. In this study, we found that geniposide could regulate the phosphorylation AMPK both in the presence of low (5.5 mM) and very high (33 mM) concentrations of glucose in INS-1, a pancreatic β cells. Furthermore, as shown in Figure 5, pre-incubation with Compound C, an AMPK inhibitor, prevented the effects of geniposide on the uptake of glucose and intracellular ATP production, which is a key driving factor for insulin release, in INS-1 cells.

Additionally, because SIRT1, an NAD-dependent deacetylase, that acts as a master metabolic sensor of NAD+, played an important role in mitochondrial biogenesis and glucose homeostasis [6,7]. We also determined the effects of geniposide on the expression of SIRT1 in INS- 1 cells. The results showed that geniposide could regulate the expression of SIRT1 in the presence of low (5.5 mM) and very high (33 mM) concentrations of glucose. Moreover, unlike the sustained activation in presence of 5.5 mM glucose, p-AMPK was fleetly decreased in the presence of 33 mM glucose, and SIRT1 expression also showed the same trend. All these observations revealed that AMPK/SIRT1, as the central regulators of cellular metabolism, were intimately linked with energy balance regulated by geniposide in presence of different concentrations of glucoses.

Furthermore, to analyze the interaction of AMPK and SIRT1 during the process of geniposide regulating glucose uptake and metabolism, Ex527 and Compound C were used in this study, the results demonstrated that Ex527 could prevent the role of geniposide on the phosphorylation of AMPK, and Compound C could prohibit the expression of SIRT1 induced by geniposide both in the presence of low and very high concentrations of glucose in INS-1 cells. So we have observed a close correlation between AMPK phosphorylation and SIRT1 level, indicating that the persistently increased the phosphorylations of AMPK and the expression of SIRT1 by geniposide in response to low glucose indicates a sequence of events in which geniposide increased the phosphorylation AMPK and the expression of SIRT1, accelerated the uptake and metabolism of glucose, promoted the production of ATP, and ultimately improved insulin secretion in pancreatic β cells, but when the cells challenged by very high concentrations of glucose, geniposide exerted a contrary role. The possible way of geniposide regulating energy balance in INS-1 cells was summarized in Figure 6.

medicinal-chemistry-geniposide-metabolic-expenditure

Figure 6: A sketch of geniposide adjusting the metabolic network of energy expenditure.

Collectively, the data presented here provide novel and in-depth evidence for the role of geniposide on pancreatic β-cells glucose and energy homeostasis. Due to the capacity of geniposide to preserve pancreatic β-cells from exhaustion resulted from prolonged and over insulin secretion under high glucose burden [17], geniposide might be used as a novel small molecular for the treatment of hyperinsulinemia, and delayedthe developmental progress of type 2 diabetes.

Acknowledgements

This work was supported by grants from Natural Science Foundation of China (81373459), Innovation of Science and Technology Leading Talent in Chongqing (CSTC2014kjcxljrc0009), Chongqing Natural Science Foundation (CSTC, 2014jcyjA10048), Innovative Research Team Development Program at the University of Chongqing (No KJTD201314), the Natural Science Startup Foundation of Chongqing Technology and Business University (1352009) and the open project of Key Laboratory of Natural Medicine Research of Chongqing Education Commission (1456032, CQCM-2015-08).

References

Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Article Usage

  • Total views: 8002
  • [From(publication date):
    January-2016 - Oct 21, 2017]
  • Breakdown by view type
  • HTML page views : 7934
  • PDF downloads :68

Review summary

  1. Faidah
    Posted on Sep 29 2016 at 6:55 pm
    Manuscript is very well organised. Significant contribution.
 

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords