alexa Power Generation by Algal Microbial Fuel Cell Along with Simultaneous Treatment of Sugar Industry Wastewater
ISSN: 2155-9821

Journal of Bioprocessing & Biotechniques
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Power Generation by Algal Microbial Fuel Cell Along with Simultaneous Treatment of Sugar Industry Wastewater

Tanisha Manchanda1*, Rashmi Tyagi1, Vijaya Kumar Nalla2, Suman Chahar3 and Durlubh Kumar Sharma4
1Department of Applied Sciences, The NorthCap University, Gurugram, Haryana, India
2Department of Microbiology, Bhaskaracharya College, University of Delhi, Dwarka, New Delhi, India
3International Academy of Environmental Sanitation and Public Health, Vijay Enclave, New Delhi, India
4Centre for Energy Studies, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, India
*Corresponding Author: Tanisha Manchanda, Department of Applied Sciences, The NorthCap University, Gurugram, Haryana, India, Tel: 0124 236 5811, Email: [email protected]

Received Date: Apr 13, 2018 / Accepted Date: Apr 18, 2018 / Published Date: Apr 25, 2018


Algal biotechnology has gained interest world over as it is a better resource in comparison to land biomass. One interesting emerging application is its utilization in microbial fuel cells (MFCs) for the production of electricity while utilizing these algae for the treatment of industrial wastewater besides hydrocarbons and lipid production later. Presently, the potential of two strains of an oil-rich green alga Botryococcus braunii has been studied for its utilization in microbial fuel cells. Photosynthetic algal strains in various media in the cathodic half of the MFCs provided a continuous source of oxygen from their photosynthesis to serve as electron acceptor. This helped in reducing the cost of energy needed for mechanical aeration to enrich the catholyte with oxygen as electron acceptor. These cathodic half cells were combined separately with three different anolytes, firstly Saccharomyces cerevisiae culture alone, secondly, Saccharomyces cerevisiae culture supplemented with the mediator methylene blue (350 mg/L) and thirdly, sugar industry wastewater mixed with activated sludge. Amongst MFCs consisting of various combinations of these catholytes and anolytes, highest values of power density (7.27 μW/cm2) and current density (20.87 μA/cm2) were observed with Saccharomyces cerevisiae culture supplemented with methylene blue as the anolyte and B. braunii in soap industry wastewater as the catholyte. Present study demonstrates the potential of the coupling of cultivation of photosynthetic B. braunii for biodiesel production with the wastewater treatment plant as a sustainable source of electricity in microbial fuel cells.

Keywords: Microbial fuel cell; Botryococcus braunii ; Saccharomyces cerevisiae ; Mediator; Wastewater treatment; Photosynthetic oxygen


Microbial fuel cell is the promising technology for clean power generation and has gained lot of attention in recent years. The algae obtained after MFC utilization can be further used for the production of biodiesel, green diesel or bioethanol [1,2]. Complete utilization of biomass is important for the transition to biofuels and bioeconomy [3]. Microbial fuel cell (MFC) consists of an anodic and a cathodic chamber, separated by a proton exchange membrane (PEM). At anode, organic compounds are oxidized by the microorganisms and electrons move towards cathode through an external circuit and combine with an electron acceptor (mainly oxygen) to generate electricity. At cathode, oxygen is supplied continuously by aeration which is an energy consuming process. In MFCs, mediators can accelerate the process of electron transfer [4]. Recently, the concepts of MFCs have been extended into different technologies such as Bio-Electrochemical Systems (BESs) which produce numerous useful products such as formate, [5] methane [6] and acetate [7]. The microbial desalination cell is the most attractive option as this can be successfully implemented for power generation, treatment of wastewater with the simultaneous desalination of water [8]. MFCs can be used for the production of hydrogen gas [9], powering environmental sensors and digital wrist watch, charging a mobile phone, smartphone and LEDs [10-13]. MFCs are also coupled with solar cells for power production [14] which provides opportunities for utilizing solar energy in this field. Future applications of MFCs may include there use in human systems as well.

Utilization of microalgae in MFCs has gained interest as phototrophic microalgae act as biocatholytes as the oxygen produced by them serves as final electron acceptor, minimizing the energy needed for the aeration at the cathode. Earlier work from author's laboratory was reported on the H2 production from algae and its utilization in carbon fuel cells for power generation [15]. Later work involved the studies of the potential of power generation from algal MFCs [2]. In the present study, potential of two strains of a green colonial hydrocarbon (petroleum oil) rich microalga, Boryococcus braunii , has been analyzed for its application in MFCs while generating biomass for oil production. In these MFCs, green algal strains have been used at the cathode as the source of photosynthetic oxygen as electron acceptor, whereas sugar industry wastewater with activated sludge, S. cerevisiae culture alone and S. cerevisiae with supplementation of methylene blue (350 mg/L) at the anode. This technology has great potential to couple the algal and yeast biomass cultivation for the oil and bioethanol production, respectively, along with wastewater treatment and power generation. Algal biomass obtained from MFCs may be used for the production of biodiesel and for bioethanol production through hydroliquefaction.

Materials and Methods

Experimental organisms

Two strains of B. braunii , Udaisagar strain from Udaisagar lake, Udaipur Rajasthan, India and Loktak strain from Loktak lake, Manipur, India, were isolated by serial dilution method and plating on the solidified Chu-13 medium [16] which were grown and maintained under controlled conditions in an incubator. Saccharomyces cerevisae (Bakers's yeast), obtained from Indian Institute of Technology, Delhi, New Delhi, India was revived by adding 1.3 gm yeast powder in 1 L Sayed et al. medium [17] for 16 h at 27°C temperature in dark.

MFC design and operation

The MFC contained two chambers, an anode and a cathode, each of 250 ml capacity with 6.5 cm diameter, 12 cm length and approximately 30 cm2 area. Both the electrodes were made up of carbon and separated by a proton exchange membrane (Nafion). A digital multimeter (Haqyue) was connected to the system for continuous measurement of voltage (V) and current (I) at the external load of 100 Ω. Peak values of current density (μA/cm2) and power density (μW/cm2) were calculated from the values of V, I and A (area of electrode). In MFCs under study, five sets of catholytes examined separately with the two strains of B. braunii were

• Algal strains in Chu-13 medium;

• Algal strains in Chu-13 medium supplemented with 0.5 M NaCl;

• Algal strains in simulated sugar industry wastewater;

• Algal strains in simulated soap industry wastewater;

• Algal strains in simulated treated sewage water.

Whereas the three anolytes used were, S. cerevisae culture in Sayed et al. medium [17]. S. cerevisae with 350 mg/L methylene blue (MB) as mediator and sugar industry wastewater (198 ml) (obtained from Saraswati Sugar Mills, Yamunanagar-135001, Haryana, India) with activated sludge (22 ml) (obtained from the sewage treatment plant Vasant Kunj, New Delhi, Delhi-110070, India). The experiment was conducted in an incubator under controlled conditions of 27 ± 1°C temperature, 1.2 ± 0.2 klux light intensity and 16L: 8D light:dark cycle to support microalgal growth and photosynthesis for 21 days which is the best harvesting time for B. braunii strains.

The working volume of microbial cultures in both the chambers of MFCs was 220 ml. During operation, the anodic chamber was covered with a black paper to avoid any exposure of light to check the growth of algae [18]. It was made anaerobic by sparging nitrogen gas before the operation and connected to a conical flask filled with distilled water through a rubber tube for the exhaustion of gases formed by the microbial metabolic activities. The mouth of the cathodic chamber was covered with a cotton plug to facilitate aeration. For both B. braunii and S. cerevisae cultures, pH was adjusted to around 7 using 1 N KOH and 1 N HCl, respectively. The schematic diagram of the experimental set up has been shown in Figure 1.


Figure 1: Schematic diagram of the experimental set up of microbial fuel cell with algal growth chamber as the cathode and yeast or sugar industry wastewater with activated sludge as the anode.

Chemical Oxygen Demand (COD) Measurement

COD of the sugar industry wastewater sample in the anodic compartment of MFC was measured on 1st and 21st days of the experiment by APHA (American Public Health Association) standard methods [19].

Simulated wastewater

Simulated treated sewage water and simulated sugar industry wastewater were prepared in the laboratory as per the composition devised by Poddar and Sahu; Yoshioka, Yadav and Yetis [20-23]. Simulated soap industry wastewater was prepared after analyzing the wastewater obtained from soap industry. Calcium, magnesium and nitrogen were analyzed by EDTA titrimetric method and semi-microkjeldahl method, respectively [19]. Phosphorus was analyzed by Olsen's method [24]. Amount of glycerol was added on the basis of the composition given by Israel et al. [25].

Results and Discussion

Earlier work in the author's laboratory was performed on the successful utilization of urine for the generation of power through microbial fuel cell [26]. However, presently it was decided to utilize algae along with sugar and soap industry wastewater in MFCs. Driving aim was to study the use of B. braunii in algal MFCs, boost the growth of algae by utilizing industrial wastewater, to treat the sugar industry wastewater in MFCs, utilize the algae for production of biofuels after its growth in MFCs and above all avoiding the use of costly hydrogen in the fuel cells. Two strains of B. braunii biomass in the cathodic compartment of the microbial fuel cells in five different media (Figure 2) were coupled with the three types of anolytes.


Figure 2: Growth of the two strains of Botryococcus braunii in different media on 21st day.

Significant results of voltage, power and current densities were obtained with all the combinations of catholytes and anolytes (Tables 1 and 2) in MFCs and the best results were obtained with S. cerevisae supplemented with the mediator methylene blue as the anolyte in combination with the five catholytes, respectively under study.

Concentration (g/L)
S. No Component Simulated sugar industry wastewater Simulated soap industry waste water Simulated  treated  sewage  water
1 K2HPO4 0.2 3.421 2.8
2 Copper (CuSO4.7H2O) 0.0333 - -
3 Calcium (CaCl2.2H2O) 1.324 1.785 0.4
4 Magnesium(MgSO4.7H2O) 2.717 0.32 0.2
5 Zinc (ZnSO4.4H2O) 0.00092 - -
6 C12H22O11 (Sucrose) 1.4372 - -
7 C3H9O3 (Glycerol) - 4 -
8 Iron (FeSO4.7H2O) 0.06372 - -
9 Oil 0.012 - -
10 KNO3 - 0.64 0.4
11 NaCl - - 0.7
12 NH4Cl 0.5 - -
13 Vegetable extract - - 11
14 Peptone - - 16

Table 1: Composition of various simulated wastewaters used for the cultivation of B. braunii strains.

Anode Parameter B.braunii in Chu-13 medium B.braunii in Chu-13 with 0.5 M NaCl B.braunii in simulated sugar industry wastewater B.braunii in simulated treated sewage water B.braunii in simulated soap industry wastewater
Udaisagar/Loktak strains
S. cerevisae in Sayed et al. medium with methylene blue (350 mg/L) as mediator Voltage (mV) 334/339 302/307 334/340 342/348 349/356
Power density (µW/cm2) 6.47/6.53 6.18/6.21 6.5/6.59 6.72/6.8 7.21/7.27
Current density (µA/cm2) 16.72/16.83 14.54/14.72 16.78/17.15 18.95/19.13 19.84/20.87
S. cerevisae in Sayed et al.  medium Voltage (mV) 256/260 223/232 259/263 264/267 269/274
Power density (µW/cm2) 2.12/2.24 1.93/1.96 2.15/2.26 2.66/2.71 2.91/3.02
Current density (µA/cm2) 10.23/10.45 9.17/9.39 10.25/10.48 10.78/10.85 10.91/10.93
Sugar industry wastewater with activated sludge Voltage (mV) 261/263 224/230 264/267 268/270 273/278
Power density (µW/cm2) 4.57/4.6 3.23/3.34 4.59/4.64 4.92/4.98 5.24/5.32
Current density (µA/cm2) 13.37/13.78 11.31/11.52 13.39/13.81 14.45/14.68 15.74/15.94

Table 2: Peak values of voltage, current and power densities obtained with various anolytes and catholytes in the microbial fuel cells.

Maximum values of power density (7.27 μW/cm2 with Loktak strain and 7.21 μW/cm2 with Udaisagar strain) and current density (20.87 μA/cm2 with Loktak strain and 19.84 μA/cm2 with Udaisagar strain) were obtained with B. braunii in soap industry wastewater as the catholyte and S. cerevisae with methylene blue as the anolyte. This might be possibly due to the best growth of algal strains in soap industry wastewater of the three catholytes used (Figure 2) and fastening of the transfer of electrons from S. cerevisae to the anode by the mediator methylene blue in the anodic chamber. Higher values of power density (5.32 μW/cm2 with Loktak strain and 5.24 μA/cm2 with Udaisagar strain) and current density (15.94 μA/cm2 with Loktak strain and 15.74 μA/cm2 with Udaisagar strain) obtained with the anolyte sugar industry wastewater with activated sludge may be due to the presence of electrochemically active bacteria in activated sludge possessing the ability to transfer the electrons to the anode [27]. The power density may be further enhanced by using food industry waste or paper industry waste in anodic compartment of microbial fuel cell.

Substantial removal of COD of the anolyte, sugar industry wastewater was also observed (COD value of 2.56 g/L was observed on 1st day whereas significant reduced values obtained on 21st day were 1.21 g/L with B. braunii in Chu-13 medium, 1.04 g/L with B. braunii in Chu-13 medium supplemented with 0.5 M NaCl, 1.42 g/L with B. braunii in simulated sugar industry wastewater, 1.3 g/L with B. braunii in simulated treated sewage water and 1.6 g/L with B. braunii in simulated soap industry wastewater, respectively). Researchers have successfully integrated various green and blue-green algae at the cathode in microbial fuel cells with significant values of current and power densities. Yadav et al. [22] have reported the peak values of current density (149.5 mA/m2) and power density (34.32 mW/m2) at the external load of 220 Ω using blue-green algae assisted cathode with 89.23% COD removal. Powell et al. [28] have reported maximum power density of 0.95 mW/m2. Jia et al. [29] used microbial fuel cell for the removal of organics and bio-electrochemical denitrification and 1.7 mW/m2 was the maximum power density obtained at a current density of 15 mA/m2. Zhang et al. [30] used photomicrobial fuel cell and produced a stable power density of 68.5 mW/m2. Authors feel that there is a great potential of using Botryococcus braunii in MFCs and for treating sugar industry wastewater.


The present study demonstrates the potential of the two strains of Botryococcus braunii as catholytes in MFCs. The MFCs were coupled with the sugar industry wastewater as anolyte which led to its successful treatment during power generation by algal MFCs. There is a future scope of utilizing urine and other industrial wastewater along with different algal species in MFCs. There is also a future scope of increasing power generation in MFCs by optimizing the relevant parameters.


Citation: Manchanda T, Tyagi R, Nalla VK, Chahar S, Sharma DK (2018) Power Generation by Algal Microbial Fuel Cell Along with Simultaneous Treatment of Sugar Industry Wastewater. J Bioprocess Biotech 8: 323. DOI: 10.4172/2155-9821.1000323

Copyright: © 2018 Manchanda T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Select your language of interest to view the total content in your interested language

Post Your Comment Citation
Share This Article
Relevant Topics
Article Usage
  • Total views: 1285
  • [From(publication date): 0-2018 - May 26, 2019]
  • Breakdown by view type
  • HTML page views: 1186
  • PDF downloads: 99