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Journal of Tropical Diseases & Public Health
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Prevalence and Risk Factors of Strongyloides Stercoralis Infection in Selected Tea Garden of Sylhet, Bangladesh

Muhammed Hossain1*, Md. Shafiul Alam2, Maisha Khair2, Md. Abu Sayeed3 and Md. Jamal Uddin Bhuiyan1

1Department of Parasitology, Faculty of Veterinary and Animal Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh

2Parasitology Research Group, Center for Communicable Diseases, International Center for Diarrhoeal Disease Research, Bangladesh

3Department of Epidemiology and Public Health, Faculty of Veterinary and Animal Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh

*Corresponding Author:
Muhammed Hossain
Department of Parasitology
Faculty of Veterinary and Animal Sciences
Sylhet Agricultural University
Sylhet-3100, Bangladesh
Tel: 880 167 306475
E-mail: [email protected]

Received Date: January 29, 2016; Accepted Date: February 06, 2016; Published Date: February 13, 2016

Citation: Hossain M, Alam MDS, Khair M, Sayeed MDA, Bhuiyan MJU (2016) Prevalence and Risk Factors of Strongyloides Stercoralis Infection in Selected Tea Garden of Sylhet, Bangladesh. J Trop Dis 4:206. doi:10.4172/2329-891X.1000206

Copyright: © 2016 Hossain M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Background: Strongyloidiasis infection is partially an asymptomatic infection and the diagnosis of patent infection is difficult using conventional parasitological methods. The residents of tea garden community of Sylhet, Bangladesh were robustly tested.
Method: The collected stool samples were tested by Harada mori culture for the presence of larval stage of Strongyloides stercoralis and to reaffirm the same samples were subjected for conventional PCR, using primer sets deigned to amplify partial ribosomal DNA of S. stercoralis genome. Finally data analysis was performed by Logistic Regression procedure using STATA 13 (College Station, Texas 77845 USA) and Pearson λ2 test, with consideration of P <0.05 as an indication of significant.
Result: A total of 300 stool samples freshly collected and examined among those 18 (06.00%) samples were found positive for S. stercoralis in Harada mori culture. In amplification of DNA extracted from raw samples and culture fluid of positive sample, the conventional PCR detected S. stercoralis 38 (12.67%) positive. There were 6 samples positive in Harada mori culture but did not show any response in sophisticated PCR techniques it might be due to low burden of infection. Periodic anthelmintic does not taking OR= 3.946(95% CI 1.369-11.375; P=0.011) and does not wash feet coming from out OR= 5.158(95% CI 1.656-16.068; P=0.005) significantly associated with Strongyloidiases infection.
Conclusion: This study confirmed that S. stercoralis is prevalent in the tea garden community of Sylhet identified by both parasitological and molecular methods. The preventive measures by deworming are warranted. Public health education regarding properly periodic anthelmintic taking, wash feet coming from out and personal hygiene are also additional required elements.

Keywords

Strongyloides stercoralis; Prevalence; Risk factors; Harada mori culture; PCR

Introduction

The threadworm Strongyloides stercoralis is a common intestinal nematode affects 30-100 million people worldwide [1,2]. S. stercoralis is only parasite of soil transmitted helminths (STH) group which can cause auto infection and thus ultimately lead to high parasite intensity specifically in immune-compromised individuals [3-5]. Strongyloidiasis is endemic in areas where sanitation conditions are poor and where the milieu is warm and humid [6] such as Asia, Africa, Southeast Asia, Bangladesh, Central and South America [7-9]. Coprological and recent serological serological studies, in slum areas of Dhaka, ensured its continued existence in Bangladesh [6,10]. Severe complication with clumsy infection of strongyloidiasis may lead to substantial mortality as high as 87% [8]. Paucity of information is available on prevalence of S. stercoralis infection on most of these setting [11]. Confirmation of Strongyloides infection by coproglogical examination is difficult because of irregular excretion of the parasite especially in chronic cases; prevalence of infection thus underestimated [9,12]. Widely used diagnostic procedures, such as direct fecal smear, Baermann technique and Koga agar plate are not satisfactory when used in single stool samples [13,14]. The detection of larvae of Stgongyloides in the stool is an evidence of infection [8]. The diagnostic methods such as direct fecal smear, Harada mori culture [15,16] have been used to detect larvae in stool but the exact sensitivity of these diagnostic approaches is debated [17]. Most of the infections may remain asymptomatic [18-20] but diarrhea and abdominal pain are the most common symptoms [21,22]. The common dermatological aspects of chronic strongyloidiasis are itching and rash [23]. There is scarcity of information on S. stercoralis infection in rural setting of tea garden community though few work available in Dhaka slum [10,24]. The objectives of this study conducted in tea garden community of Sylhet to ascertain the prevalence and plausible determinants for strongyloidiasis including socio demographic and household factors based on Harada mori culture and molecular techniques.

Materials and Methods

Study area

The study areas of Sylhet district located 315 km south east from capital city Dhaka were selected for this study as it is the poorest area of Bangladesh. Sylhet is located at 24.8917°N and 91.8833°E. It has 86074 units of house hold and total area 323.17 km2; [25]. This district is occupied by high proportion of ethnic minorities, stingy household condition, and poor road condition, no prohibition for preventive and curative measures.

Ethical Consideration

Before commencement of the study, ethical clearance was obtained from the SAU Ethical Review Board. A consent form was provided to each study subject together with stool containers a day before the day of data collection. Parents were asked to sign the consent forms if they agreed on their children to be involved in the study.

Data collection

The study was conducted for a period of 12 months starting from June 2014 to May 2015. Before enrollment of the participants the verbal consent of the parents or legal guardians was taken. Data were collected by following structured questionnaire approved by ICDDR’B, Dhaka, Bangladesh.

Collection of stool sample

Supply and collection of stool pot: During each phase of study appropriately labeled plastic stool container for the collection of stool specimen was provided to the parents. The label of the stool pot had the subject’s name, date of sample collection, identification number and the name of the areas. They were instructed on how to collect and put stool in the container at the toilet. The next day morning the stool pot collected directly from the participant’s guardians with making proper questionnaire. The specimens were packed in a cool box with ice packs and transported by a vehicle to the Parasitology Laboratory, Sylhet Agricultural University, Faculty of Veterinary and Animal Science, Sylhet, Bangladesh and for molecular detection sent to parasitology laboratory of International Center for Diarrhoeal Disease Research Bangladesh (ICDDR’B), Dhaka, Bangladesh.

Analysis of stool specimens

Direct smear method and Harada mori culture: The stool samples collected from each participant were examined direct saline smear for the presence of parasitic eggs. The every stool sample were cultured at 25-28°C in incubator for ten days and examined from five days onward up to ten days to evaluate the presence of larvae in culture fluid [26]. Any sample shows positive either in direct smear or Harada mori culture was considered as positive sample. The extracted DNA from filariform larvae was used as control DNA for molecular analysis.

Extraction of genomic DNA

For the isolation of DNA from positive culture water, QIAGEN DNeasy Blood & Tissue Kit (QIAGEN, Hilden, Germany) was used. The culture positive water was vortexed and centrifuged then diluted by filtered PBS. 200 μl of sample was taken into 1.5 ml micro-centrifuge tubes and 20 μl Proteinase K was added. The tubes were vortexed by kept for incubation for 10mins at room temperature. 200 μl of buffer ATL was added to the tubes and mixed well. The samples were incubated in a water bath at 56°C for 1 hour. 200 μl of 100% Et-OH was added to the tubes. The samples were then transferred to the spin columns. The spin columns were centrifuged at 8,000 rpm for 1 min. Collection tubes were changed. 500 μl of AW1 buffer was added to the columns. Again centrifuged at 8,000 rpm for 1 min. The liquid was removed from the collection tubes and placed back. 500 μl of AW2 buffer was added to the columns. Centrifuged at 14,000 rpm for 3 min. The columns were placed in a fresh micro-centrifuge tube. 60 μl of AE buffer (elution) was added. Centrifuged at 14,000 rpm for 2 min. The columns were discarded and the DNA was ready.

Conventional PCR

Purified DNA template was used for amplification in a DNA thermal cycler using a species specific primer set as described by [27]. Positive and negative controls were systematically incorporated in each PCR run. Forward (SSF: 5´ATC GTG TCG GTG GAT CAT TC 3´) and reverse (SSR: 5´CTA TTA GCG CCA TTT GCA TTC 3´) primer pair was used and target 114bp gene. PCR reactions were performed using the following reaction mixture. 2 μl of purified DNA template was used for the PCR with 3 μl of each primer, 2.5 μl of 10X Taq buffer (New England Biolabs Inc.), 0.5 μl of 10 mM dNTPs (GENE Mate), 2 μl of 25 μM MgCl2 and 0.2μl of Taq DNA Polymerase (New England Biolabs Inc.) In total volume of 25 μl reaction mixture. The cycle conditions for the PCR (40 cycles) step with an initial denaturation period of 5 min at 95ºC were: denaturation at 95°C for 40 sec, annealing at 50°C for 40 sec, extension at 68°C for 20 sec and final extension for 8 min to insure that all product were full-length. The amplified PCR products were analyzed immediately by electrophoresis on a 2.0% agarose gel (Sigma -Aldrich Inc., USA).

Electrophoresis

The amplified PCR products were analyzed immediately by electrophoresis on a 2.0% agarose gel (Sigma -Aldrich Inc., USA). The gel was stained with ethidium-bromide and visualized under UV transillumination (GelDoc®, Biorad, USA). The sizes of the PCR products were estimated using 100 base pairs (bp) DNA ladder marker (Sigma- Aldrich Inc., USA).

Statistical analysis

Statistical analysis was performed by Logistic Regression procedure using STATA 13 (College Station, Texas 77845 USA) and the level of significance was considered as P<0.05. In the univariable analysis, λ2 test done for risk factors associated with infection status. In the multivariable logistic regressions, variables with a P-value of ≤0.20 in the univariable analysis were included as predictors. ORs and 95% CI were reported. P-values <0.05 were regarded as significant.

Results

Study participants

Of 300 participants 59.00% male and 41.00% female were enrolled in this study out of five tea garden areas. Only 40.00% of the participants had primary education whereas majority of the participants 60.00% had not received primary education. The primary education completed possesses 10.56% (95% CI 06.476-15.992) infection and illiterate participant’s 15.83% (95% CI 09.8102-23.616) infection.

Prevalence of S. stercoralis infection

Of 300 tested sample only 38 cases found positive with S. stercoralis infection. The prevalence of strongyloidiasis is decreasing with the increase of ages though there is increase in the age group 21-30 years. From all ages group prevalence was higher in female 13.82% (95% CI 08.262-21.204) than male 11.86% (95% CI 07.496-17.562) with S. stercoralis infection.

Foot ware is a factor for S. stercoralis infection because having no foot ware showing higher prevalence than having foot ware. The prevalence is higher in winter season 18.97% (95% CI 12.283-27.293) than rainy 15.38% (95% CI 07.632-26.478) and summer 05.04% (95% CI 01.872-10.651). The crowd house have higher rate of infection than small family (Tables 1- 4).

Characteristics Frequency Total no. of sample Percentage
Participants Schooling   300  
Primary 104   34.67
Secondary 16   05.33
Not applicable 180   60.00
Mother’s schooling   300  
Primary 63   21.00
Secondary 03   01.00
Not applicable 234   78.00
Father’s schooling   300  
Primary 86   28.67
Secondary 72   24.00
Not applicable 142   47.33

Table 1: Educational status of the participants and parents in tea garden community.

Level of occupation Frequency Total no. of sample Percentage
Father’s occupation   300  
Unemployed 11   03.67
Day laborer 182   60.67
Service 80   26.67
Tea garden worker 9   03.00
Other 18   06.00
Mother’s occupation   300  
Housewife 167   53.33
Service 1   00.33
Business 132   44.00

Table 2: Occupational status of the parents in tea garden community.

Other demographic information Frequency Total no. of sample Percentage
Age group   300  
0-10 242   80.67
11-20 19   06.33
21-30 13   04.33
31-40 12   04.00
41-up 14   04.67
Sex group   300  
Male 177   59.00
Female 123   41.00
Name of the areas   300  
Khadim tea estate 47   15.67
Burjan tea estate 79   26.33
Lakkatora tea estate 65   21.00
Malnichara tea esstate 63   21.67
Daldali tea estate 46   15.33
Category of weight  (kg)   300  
0-20 186   62.00
21-40 65   21.67
41-up 49   16.33
Income of house   300  
≤5000 103   34.33
>5000 197   65.67
No of People living in house   300  
≤4 145   48.33
≥5 155   51.67
Name of the season   300  
Rainy 65   21.67
Winter 116   38.67
Summer 119   39.67

Table 3: Other demographic information of tea garden community.

Points Total no. of tested Total no. of positive Prevalence  (95% confidence Intervals)
Season      
Rainy 65 10 15.38 (07.632-26.478)
Winter 116 22 18.97 (12.283-27.293)
Summer 119 06 05.04 (01.872-10.651)
Age      
≤10 242 32 13.22 (09.223-18.151)
11-20 19 01 05.26 (00.133-26.028)
21-30 13 02 15.38 (01.920-45.447)
31-40 12 01 08.33 (00.210-38.479)
≥41 14 02 14.29 (01.779-42.812)
Areas      
Khadim tea estate 47 12 25.53 (13.944-40.349)
Burjan tea estate 79 13 16.46 (09.063-26.494)
Lakkatora tea estate 65 05 07.69 (02.544-17.045)
Malnichara tea estate 63 08 12.69 (05.645-23.496)
Daldali tea estate 46 - -
Sex      
Male 177 21 11.86 (07.496-17.562)
Female 123 17 13.82 (08.262-21.204)
People in house      
≤4 127 18 14.17 (08.621-21.471)
≥5 135 20 14.81 (09.290-21.948)
Have foot ware      
No 05 02 40.00 (05.274-85.336)
Yes 257 36 14.01 (10.007-18.862)
Work in bare foot      
No 48 07 14.58 (06.070-27.764)
Yes 214 31 14.49 (10.059-19.927)

Table 4: Prevalence of Strongyloides stercoralis infection in tea garden community.

Overall, in examination by conventional PCR, 38 out of 300 stool samples were found positive for S. stercoralis where by Harada mori culture for the same samples the detected positive cases was 18 (Table 5).

Name of the test Harada Mori Culture Total
  Positive ­­­­Negative  
Conventional PCR Positive 12 26 38
Negative 6 256 262
Total 18 282 300

Table 5: Comparison of the results of conventional PCR and Harada mori culture examinations for detection of Strongyloides stercoralis infection in single stool samples.

Risk factor assessment for S. stercoralis infection

The study participants corresponding data were analyzed by λ2 test for univariable analysis and found several factors significantly associated with strongyloidiasis infection. The climatic factors such as season (P=0.004) and areas (P=0.003) were significantly associated.

Other factors such as periodic anthelmintic therapy, monthly family income, rubbing hand after toilet, toilet floor and using shoes in toilet are contributing tools for strongyloidiasis infection (Table 6). On the other hand sex, fathers occupation, household floor, disposal of stool, use of disinfectant cleaning toilet, possession of foot ware and working in bare foot are not significantly associated with Strongyloidiasis infection (Table 7).

Characteristics Positive n=38 (%) Negative n=262 (%) P-value
Season      
Rainy 10 (15.38) 55 (84.62)  
Winter 22 (18.97) 94 (81.03)  
Summer 06 (05.04) 113 (94.96) 0.004
Areas      
Khadim tea estate 12 (25.53) 35 (74.47)  
Burjan tea estate 13 (16.46) 66 (83.54)  
Lakkatora tea estate 05 (07.69) 60 (92.31)  
Malnichara tea esstate 08 (12.70) 55 (87.30)  
Daldali tea estate 0.00 46 (100.0) 0.003
Sex      
Male 21 (11.86) 156 (88.14)  
Female 17 (13.82) 106 (86.18) 0.616 (NS)
Weight      
<10 21 (11.29) 165 (88.71)  
11-20 11 (16.92) 54 (83.08)  
>21 06 (12.24) 43 (87.76) 0.449 (NS)
Participant’s schooling      
Not applicable 19 (15.83) 101 (84.17)  
Primary 19 (10.56) 161 (89.44) 0.050
Father’s occupation      
Unemployed 02 (18.18) 09 (81.82)  
Day laborer 24 (13.19) 158 (86.81)  
Service 05 (06.25) 75 (93.75)  
Tea garden worker 02 (22.22) 07 (77.78)  
Other 05 (27.78) 13 (72.22) 0.100 (NS)
How many time pass stool      
Two 22 (26.83) 60 (73.17)  
One 16 (07.34) 202 (92.66) <0.001***
Monthly family income      
≤5000 19 (18.45) 84 (81.55)  
>5000 19 (09.64) 178 (90.36) 0.030
Receive treatment      
No 26 (20.80) 99 (79.20)  
Yes 12 (06.86) 163 (93.14) <0.001***
Treatment 4 month interval      
No 34 (19.43) 141 (80.57)  
Yes 04 (03.20) 121 (96.80) <0.001***
Household floor      
Mud 38 (13.82) 237 (86.18)  
Semi-cemented 0.00 24 (100.0)  
Cemented 0.00 01 (100.0) 0.138 (NS)
Toilet floor      
Mud 20 (09.95) 181 (90.05)  
Bamboo 18 (18.18) 81 (81.82) 0.044
Where dispose stool      
Around house 24 (11.37) 187 (88.63)  
Jungle/Tea garden 14 (15.73) 75 (84.27) 0.300 (NS)
Rub hand after toilet      
No 28 (10.94) 228 (89.06)  
Yes 10 (22.73) 34 (77.27) 0.030
Material for rubbing hand      
Soap 01 (25.00) 03 (75.00)  
Ash 12 (19.35) 50 (80.65)  
Soil 15 (07.89) 175 (92.11)  
other 10 (22.73) 34 (77.27) 0.012
Use disinfectant toilet cleaning      
No 13 (56.76) 61 (43.24)  
Yes 29 (36.73) 197 (63.27) 0.308 (NS)
Type of material washing hand      
Soil 13 (22.41) 35 (74.47)  
Ash 13 (06.67) 182 (93.33)  
Soap 12 (25.53) 45 (77.59) <0.001***
Wash feet using toilet      
Never 30 (14.25) 182 (85.85)  
Not always 04 (05.13) 74 (94.87)  
Always 04 (40.00) 06 (60.00) 0.004
Have foot ware      
No 02 (28.57) 05 (71.43)  
Yes 36 (12.29) 257 (87.71) 0.200 (NS)
Use shoe going to school      
Never 22 (10.58) 186 (89.42)  
Rarely 12 (27.27) 32 (72.73)  
Most time 04 (08.33) 44 (91.67) 0.005
Work in bare foot      
No 07 (12.73) 48 (87.27)  
yes 31 (12.65) 214 (87.35) 0.988 (NS)
Wash feet coming from out      
Not always 22 (17.19) 106 (82.81)  
Always 16 (09.30) 156 (90.70) 0.042

Table 6: Invariable analysis of the factors associated with Strongyloides stercoralis infection in tea garden community of Sylhet.

Name of the variable Odds ratio  (95% Confidence Intervals) P-value
Season    
Rainy 3.424 (1.183-9.905) 0.023
Winter 4.407 (1.716-11.320)  
Summer 1  
Participants Schooling    
Not applicable 2.923  (1.096-7.793) 0.032
Primary 1  
Income    
≤5000 2.540 (0.970-6.649) 0.051
≥5000 1  
Receive anthelmintics    
No 3.946 (1.369-11.375) 0.011
Yes 1  
Receive anthelmintic 4 month interval    
No 3.812 (1.079-13.464) 0.038
Yes 1  
Toilet floor    
Mud 2.710 (1.035-7.101) 0.042
Bamboo 1  
How many time pass stool a day    
Two 3.645 (1.385-9.593) 0.009
One 1  
Wash feet using toilet    
Never 12.33 (2.449-62.100) 0.043
Not always 3.049 (1.038-8.959)  
Always 1  
Type of material washing hand    
Soil 3.431 (1.422-8.276) 0.006
Ash 02.80 (1.231-6.367)  
Soap 1  
Going to School    
Never 5.556 (1.959-15.753) <0.001***
Rarely 5.000 (0.937-26.683)  
Most time 1  
Wash feet coming from out    
Not always 5.158 (1.656-16.068) 0.005
Always 1  

Table 7: Multivariable analysis of Strongyloides stercoralis infection in Tea garden community of Sylhet.

Discussion

Of three hundred stool samples of tea garden community of Sylhet were screened by Harada mori Culture and Conventional PCR found prevalence of S. stercoralis infection by copro culture 06.00% (95% CI 03.83-09.28) and conventional PCR 12.67% (95% CI 09.37- 16.91) which is almost double than copro culture. These reports have coherence with [28,29] but contracted with recent study in Dhaka City [10]. The difference of the infection rate is also statistically significant. The previous reports from Thailand revealed that the prevalence of S. stercoralis infection varied widely and ranging between 07.60% and 30.30% [10,30,31]. This is the first time study on Strongyloidiases in tea garden community of Sylhet and there is paucity of information about it. The present study showed female (13.82%) participants have higher prevalence of S. stercoralis infection than male (11.86%) which is also supported by [32]. The winter season disclosed highest percentage of infection whereas rainy stood second and summer stood lowest infection. This variation of infection might be due to the environmental factors stimulating development of the parasitic larvae. The Khadimnagar Tea garden’s hygienic condition was very poor compared to other tea garden and its parasitic load were highest but one novel findings of our study is Daladali tea garden is free from S. stercoralis infection.

The elderly persons had higher prevalence than the young participants in our study which is supported by another study in Cambodia [11].

The risk factors of strongyloidiasis increased with illiteracy OR= 2.923(95% CI 1.096-7.793, P= 0.032) which is similar to the finding of [32] in Ethiopia. The climatic factor such as rainy season OR= 3.424(95% CI 1.183-9.905, P= 0.023) associated with infection than other season. Periodic anthelmintic taking reduces the infection rate but when break in the chemotherapy of four month interval occurs then high rate of infection OR= 3.812(95% CI 1.079-13.464, P= 0.038) revealed. The family income significantly associated with Strongyloidiases infection because the low income family gets more infection owing to their poor family status and unable to keep them in hygienic condition. One study in elderly of Brazil Showed significantly association of family income with Strongyloidises infection [33].

Personal hygiene wash feet using toilet, coming from out significantly associated with Strongyloidiasis infection. Types of material for washing hand significantly contribute to the infection, use of soil 3.431 fold (1.422-8.276), use of ash 2.8 fold (1.231-6.367) risk for S. stercoralis infection. Muddy toilet floor OR= 2.710(95% CI 1.035-7.101, P= 0.042) contribute times higher strongyloidiases than Bamboo floor because the muddy floor provides suitable condition for the development of the infective larvae. When these infective larvae get contact with the intact skin then penetrate the skin initiate its infection in the host body. Washing practices of feet coming from out was important factors for Strongyloidases infection, the participants who did not washed feet get infection OR= 5.158(95% CI 1.656-16.068, P=0.005.

The lack of correlation between copro-culture and Molecular techniques for detection of S. stercoralis infection in our study population might indicate that the two methods are detecting different percentages of infected individuals. Those who were not detected positive by copro-culture showed positive in molecular techniques. There were 18 samples positive in copro-culture and 38 samples were positive in molecular techniques. Nevertheless, our study shows that S. stercoralis infection remains prevalent in Bangladesh particularly in tea garden community.

Conclusion

We can conclude that S. stercoralis infection was highly prevalent in the tea garden community of Sylhet and it does not depend on whether the individual was institutionalized or not. Therefore early diagnosis through specific methods is warranted in asymptomatic elderly in order to prevent the risk of hyper-infection or disseminated infection, thus avoiding high mortality.

Acknowledgements

The Authors thankful to International Center for Diarrhoeal Disease Research Bangladesh (ICDDR’B), Dhaka, Bangladesh for their logistic supports and providing laboratory facilities. We also thank the Department of Parasitology, Sylhet Agricultural University, Sylhet. We also acknowledge the contribution of the study participants.

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