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ISSN: 2155-9864
Journal of Blood Disorders & Transfusion
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Prevalence and Trends of Hepatitis B, Hepatitis C and Human Immunodeficiency Viruses among Blood Donors in Islamabad, Pakistan 2005-2013

Hasan Abbas Zaheer1*, Umar Saeed2, Yasir Waheed2, Saira Karimi3 and Usman Waheed1

1Safe Blood Transfusion Programme, Government of Pakistan, Blood Transfusion Services, Pakistan Institute of Medical Sciences, Islamabad, Pakistan

2Atta-ur-Rahman School of Applied BioSciences (ASAB), National University of Sciences and Technology (NUST), Pakistan

3Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan

*Corresponding Author:
Hasan Abbas Zaheer
Blood Transfusion Services
Pakistan Institute of Medical Sciences, Islamabad, Pakistan
Tel: 0092 333 512 38 14
Fax: 0092 51 926 3238
E-mail: [email protected]

Received date: March 21, 2014; Accepte date:d April 29, 2014; Published date: May 07, 2014

Citation: Zaheer HA, Saeed U, Waheed Y, Karimi S, Waheed U (2014) Prevalence and Trends of Hepatitis B, Hepatitis C and Human Immunodeficiency Viruses among Blood Donors in Islamabad, Pakistan 2005-2013. J Blood Disord Transfus 5:217. doi: 10.4172/2155-9864.1000217

Copyright: © 2014 Zaheer HA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Background: Pakistan is a developing country of 190 million people with increased burden of infectious diseases. Hepatitis B virus (HBV), Hepatitis C virus (HCV) and Human Immunodeficiency virus (HIV) are the most important agents responsible for transfusion transmitted viral infections. Blood donors are considered as the healthiest population of a community and screening of HBV, HCV & HIV in blood donors will reflect the true prevalence of these infections in a population.

Material and Methods: The study was conducted from July 2005 to July 2013 at Pakistan Institute of Medical Sciences (PIMS) hospital, Islamabad, Pakistan. 160,376 blood donors from the age 18 years to 60 years were enrolled in this study. Study was approved by the ethical review committee of PIMS hospital. All the samples were screened for Hepatitis B virus surface Antigen (HBsAg), anti-HCV and anti-HIV by fourth generation ELISA. Statistical analyses were carried out by using SPS software version 17.

Results: Among 160,376 donors, 157,920 (98.47%) were replacement donors. The overall seroprevalence of (HBVsAg), anti-HCV and anti-HIV among blood donors were 2.35%, 3.26% & 0.017% respectively. The prevalence of HBV and HCV co-infection was 0.084%.

Conclusion: The prevalence of Hepatitis B, Hepatitis C and Human Immunodeficiency viruses are high in blood donors and should not be acceptable. There is a need to educate masses to increase the number of voluntary donors to meet the country’s requirement of blood donation

Keywords

Hepatitis B virus; Hepatitis C virus; Human Immunodeficiency virus; Blood donors; Pakistan

Introduction

Hepatitis B virus (HBV), Hepatitis C virus (HCV) and Human Immunodeficiency virus (HIV) are three most important agents responsible for Transfusion Transmitted Infections (TTIs), worldwide. Globally, the World Health Organization (WHO) has estimated 2 billion people living with HBV, 200 million people infected with HCV and 33.4 million people infected with HIV [1-4]. The Centre for Disease Control and Prevention, USA (CDC) has reported HBV to be 10 and 100 times more infectious, compared to HCV and HIV respectively [5]. HBV infected patients are more likely to develop Hepatocellular carcinoma (HCC), compared to patients with HCV [6]. In a retrospective study conducted between 1992 and 2006 on 128,726 patients, the mortality rates due to HCV mono infection, HBV mono infection, HBV/HCV co-infection, HIV/HBV co-infection, HIV/HCV co-infection and HIV/HBV/HCV triple infection were 63.7%, 33%, 2.6%, 0.2%, 0.5%, and <0.1% respectively [7].

In Pakistan, HBV infection rate is steadily increasing with an estimated nne million people currently living with HBV [8]. More than ten million people are HCV infected in Pakistan. According to an estimate, approximately 97,000 to 1, 25,000 persons are HIV Positive in Pakistan [9]. The prevalence of HBV, HCV, and HIV in blood donors of Pakistan has been reported to be 2.33%, 3.78%, and 0.06% respectively [10-12]. Pakistan, a developing country with 190 million inhabitants, thus faces a high socioeconomic burden of infectious diseases. This is in addition to high prevalence of anemia, communicable diseases, malnutrition, thalassemia, obstetrical emergencies, traumatic injuries and road traffic accidents. In such circumstances, the demand for blood supply increases, and blood transfusion becomes lifesaving. According to an estimate, 3 million blood donations are carried out each year in Pakistan but unfortunately the country suffers shortage of blood and blood components [13]. In Islamabad, although the government is providing better health facilities to common people, yet due to lack of awareness among general public and non-implementation of health related safety standards at various hospitals, the prevalence of infectious diseases (HBV, HCV and HIV) is progressively increasing.

Due to the imbalance in the supply and demand, the quality of blood transfusion services has been adversely affected. The total number of blood banks is estimated to be 1830 with majority (85.8%) of them in the private sector [13]. Most of the blood transfusion services are confined to hospitals, with no functional separation of the processes into production and utilization while majority of blood donations are offered by replacement blood donors. Screening for transfusion-transmissible infections (TTIs) is a critical part of the process of ensuring blood safety. Unsafe blood transfusion is very costly both from a human and an economic point of view. Inadequate blood supply has led to increase in reliance on replacement donations and in some centers to even paid donations. In addition, the cultural and socio economic factors are associated with reluctance to donate blood as solidarity is still ‘biradari’ based and normally takes the form of family replacement donation with blood being collected “just on time” in a hospital environment with no arrangement for donor or community interface management. In general, this model meets the demand, though at the potential risk of increased TTI infection rate [14]. Reporting of prevalence data on transfusion transmitted diseases among blood donors can provide an insight into the trends towards blood borne infections in blood donor populations and consequently determine safety of the collected blood donations. The aim of our study was to determine trends in prevalence of HBV, HCV and HIV infections during last eight years (2005-2013 years), among blood donors of capital cities (Islamabad and Rawalpindi) of Pakistan.

Materials and Methods

A cross sectional study was conducted during the period of July 2005 to July 2013 among 160,376 health blood donors aged 18 to 60 at the Pakistan Institute of Medical Sciences (PIMS), Islamabad. PIMS is tertiary care teaching hospital that provides healthcare facilities to over 5 million inhabitants of Islamabad-Rawalpindi metropolitan area, adjoining areas and north of the country.

The ethical committee of Pakistan Institute of Medical Sciences (PIMS) approved the study proposal and protocol. Appropriate clinical examination for each participant was conducted while individuals with severe health complications were excluded from study. Informed written consent, medical and socio-demographic histories and donor history questionnaire containing previous history of blood transfusions and risky sexual behaviors of donors were collected from each enrolled participant.

ABO and Rhesus (Rh) blood typing were carried out by using monoclonal blood grouping antiserum; included anti-A, anti-B, anti- AB and anti-D. All donations were tested according to the standardized screening test algorithms for hepatitis B surface antigen (HBsAg), anti-HCV and anti-HIV. Initial screening was performed by fourthgeneration ELISA kits (Bio-kit). All reactive samples were repeated in duplicate. Repeat reactive sera were recognized as confirmed ELISA positive cases. The positive blood units were incinerated. Statistical analysis was carried out via Statistical Package for Social Sciences (SPSS) software version 17. Significance value was evaluated at 0.05 levels with 95% confidence level.

Results

The results of serological screening tests for HBV, HCV and HIV performed within the duration of 2005 to 2013, revealed continuously increasing trend of all infections. Among 160,376 healthy blood donors, 159,770 (99.62%) were males and 606 (0.38%) were females. The replacement blood donors were 157,920 (98.47%) while volunteer blood donors were only 2,456 (1.53%). The overall Seroprevalence of HBsAg, anti-HCV and anti-HIV among blood donors of Islamabad- Rawalpindi metropolitan area, for the duration of last eight years, were 3,776 (2.35%), 5,222 (3.26%) and 27 (0.017%) respectively. HBV and HCV co-infection was observed among 135 participants with an overall prevalence of 0.084%.

For analysis of change in trend, the data was segregated into group A comprising of cases from 2005 to 2009 (n=71,896) and group B (n=88,480) spanning from 2010 till 2013. Analysis revealed a significant increase in prevalence of HBsAg, HCV and HIV respectively. The prevalence of HBsAg rose from 1.856% (1339 out of 71,896) in group A to 2.75% (2,437 out of 88,480) in group B (P<0.001). Similarly significant difference was observed between the two groups for prevalence of HCV and HIV (3.14% vs. 3.35%; P=0.005 for HCV and 0.0069% vs. 0.025%; P<0.001 for HIV) respectively. The prevalence of HBV/HCV co-infection increased from 0.078% in group A to 0.09% in group B, during this period. The Figure 1 shows prevalence of HBsAg, Anti-HCV and Anti-HIV in blood donors during 2005-2013, while Table 1 represents type of donors and gender distribution.

blood-disorders-transfusion-Blood-Donors

Figure 1: Prevalence of HBsAg, Anti-HCV and Anti-HIV in Blood Donors.

 Sr. No Year Total Donors Volunteer (%) Replacement (%) Male (%) Female (%)
1 2005-6 15,213 1,374 (9.03%) 13,839 (90.97%) 14,907 (98.0%) 306 (2.0%)
2 2006-7 19,374 96 (0.50%) 19,278 (99.50%) 19,333 (99.8%) 41 (0.2%)
3 2007-8 18,241 210 (1.15%) 18,031 (98.85%) 18,207 (99.8%) 34 (0.2%)
4 2008-9 19,068 313 (1.64%) 18,755 (98.36%) 19,022 (99.8%) 46 (0.2%)
5 2009-10 19,862 187 (0.94%) 19,675 (99.06%) 19,794 (99.7%) 68 (0.3%)
6 2010-11 21,825 136 (0.62%) 21,689 (99.38%) 21,780 (99.8%) 45 (0.2%)
7 2011-12 23,377 46 (0.20%) 23,331 (99.80%) 23,346 (99.9%) 31 (0.1%)
8 2012-13 23,416 94 (0.40%) 23,322 (99.60%)    23,381(99.85%) 35 (0.15%)
   Total 8 Years 160,376 2,456 (1.53%) 157,920 (98.50%) 159,770 (99.62%) 606 (0.38%)

Table 1: Type of donors and gender distribution.

Discussion

As a result of weak governance and regulation, the transfusion services in the country are not quality assured and standardized. The Blood Transfusion Authorities, regulatory bodies for inspection, licensing and data management, have been notified in all provinces but are still in their formative years. Transfusion transmitted infections are frequently detected among paid blood donors. Many developed nations have outlawed the use of paid blood donors in order to minimize the risk of transfusion-transmitted diseases. Since 1998, Chinese government banned paid blood donations and currently many blood banks are relying on voluntary blood donations. This strategy has significantly contributed in decline of high HCV prevalence from 8.68% (1990) to 3.2% (2010) in general population of China [15]. India, Pakistan’s neighbor has also passed a law in 1998 to ban all paid donations and successfully managed to reduce prevalence of TTIs among blood donors. It has been reported that percentage prevalence of HBV, HCV and HIV in India, among blood donors reduced from 1.62%, 1.85% and 1.16% (in 2004) to 0.92%, 0.52% and 0.21% (in 2009) respectively [16]. Iran, Pakistan’s neighbor has also managed to reduce percentage prevalence of TTIs among blood donors during 2003 to 2005. In Iran, the prevalence of HBV, HCV and HIV was 0.71%, 0.14% and 0.0033% respectively (in 2003), while its prevalence reduced to 0.48%, 0.023% and 0.0031% respectively (in 2005). In Afghanistan, 1, 25,832 blood donors were tested during 1989 to 2005. Among those individuals, 1.76% and 0.63% were positive for HBsAg and Anti-HCV respectively [17].

According to Anwar et al, the prevalence of HBsAg and Anti-HCV was observed 7.94% and 2.79% respectively [18]. This study indicates constantly increasing burden of HBV, HCV and HIV among blood donors during the last eight years. Our study has identified that during the start of study (i.e. during 2005-6), among 15,213 blood donors, the prevalence of HBsAg, Anti-HCV and Anti-HIV were 1.68%, 2.96% and 0% respectively but during 2012-13 the prevalence of HBV, HCV and HIV among 23,416 blood donors increased up to 3.78%, 3.17%, 0.08% respectively. In Pakistan, with an only incipient culture of voluntary donations, a strong reliance on replacement and the lack of systematic screening, the infection risks are increased, as family replacement donors are more likely to transmit transfusion-transmissible infections than are voluntary donors. Replacement blood donors are usually relatives and close friends who may hide their actual health status, in order to save lives of their loved ones [14]. For a nation to reach its basic blood requirements, at least 1% of population should donate blood [19]. It is estimated that average donation rate is only 0.37% and 0.75% in developing and transitional countries, compared to 3% in developed countries. The average number of blood donations per 1000 persons in low-income countries is 10 times lower as compared to the blood donations from developed countries [14,19,20].

Our data indicates gradual decrease in trend of volunteer blood donations during previous eight years. During 2005-6, 1,374 (9.03%) volunteer blood donations were received, but after eight years, the number of voluntary blood donations decreased to 94 (0.40%). Similarly, our data indicates gradual increase in reliance on replacement blood donations. During 2005-6, 13,839 (90.97%) replacement blood donors were enrolled, but during 2012-13, their number increased to 23,322 (99.60%). Current study indicates that during last eight years 98.46% were replacement blood donors, while voluntary blood donors were only 1.53%. This data also suggests that with an increase in replacement blood donations and decreasing trend of voluntary blood donations, the percentage prevalence of transmission transmitted infections (HBV, HCV and HIV) also increases. In Pakistan, limited data on prevalence of HIV has been reported. The prevalence of HBV and HCV among blood donors varies in different regions of Pakistan as shown by Table 2.

Place of Study Year of Study Author HBsAg Positive% Anti-HCV Positive % Population Type References
Lahore 2000 Majed et al. 2.10%   Blood Donors [22]
Lahore 2002 Rahman et al. 2.25% 4.10% Blood Donors [23,24]
Lahore 2005 Sirhindi et al. 3.36% 4.16% Blood Donors [25]
Lahore 2007 Ijaz et al. 1.52% 5.34% Blood Donors [26]
Lahore 2007 Sultan et al. 1.46-2.99% 3.01-4.99% Blood Donors [27]
Lahore 2009 Manzoor et al. 1.70% 7.69% Blood Donors [28]
Lahore 2011 Anwar et al. 2.79% 7.94% Blood Donors [18]
Karachi 2000 Mujeeb et al. 2.21% 0.30% Blood Donors [29]
Karachi 2004 Akhtar et al.   1.80% Blood Donors [30]
Karachi 2006 Mujeeb et al. 4.70% 3.60% Blood Donors [31]
Karachi 2007 Azam et al. 4.50% 4.36% Blood Donors [32]
Karachi 2008 Mujeeb et al. 6.20% 7.50% Blood Donors [33]
Karachi 2008 Nazar et al. 1.71% 2.06% Blood Donors [34]
Karachi 2004-11 Irfan et al. 1.90% 2.61% Blood Donors [35]
Peshawar 2000 Ahmed et al. 1.55%   Blood Donors [36]
Peshawar 2004 Zaidi et al. 1.40% 1.34% Blood Donors [37]
Peshawar 2004 Ahmed et al. 1.90% 2.20% Blood Donors [38]
Peshawar 2007 Farooqi et al. 2.54% 3.21% Blood Donors [39]
Peshawar 2008 Khattak et al.   4.10% Blood Donors [40]
Peshawar 2011 Khan et al. 2.07% 0.89% Blood Donors [41]
Peshawar 2011 Khan et al.   1.89% Blood Donors [42]
Rawalpindi 2002 Mumtaz et al. 5.86% 6.21% Blood Donors [43]
Rawalpindi 2002 Khattak et al. 3.30% 4.00% Blood Donors [44]
Rawalpindi 2006 Chaudhary et al. 2.45% 2.52% Blood Donors [45]
Rawalpindi 2006 Bhatti et al. 2.16%   Blood Donors [46]
Rawalpindi 2007 Khan et al. 2.31% 4.87% Blood Donors [47]
Bahawalpur 2002 Fayyaz et al. 7.53%   Blood Donors [48]
Bahawalpur 2006 Fayyaz et al. 2.69% 2.52% Blood Donors [49]
Quetta 2003 Ali et al.   1.87% Blood Donors [50]
Quetta 2007 Khan et al. 4.80% 1.80% Blood Donors [51]
Multan 2004 Mehmood et al. 3.37% 0.27% Blood Donors [52]
Islamabad 2004 Asif et al. 2.51% 5.14% Blood Donors [53]
Islamabad 2009 Waheed et al. 1.92% 3.31% Blood Donors [54]
Skardu 2006 Aziz 8.40% 1.10% Blood Donors [55]
Liaquetpur 2006 Khan et al. 5.96% 0.07% Blood Donors [56]
Swat 2006 Ahmad 1.11% 2.23% Blood Donors [57]
South Punjab 2007 Jehangir et al. 4.93% 4.06% Blood Donors [58]

Table 2: Prevalence of HBV and HCV among blood donors of different cities of Pakistan.

According to six different studies from Lahore (between 2000- 2011), the prevalence of HBV among blood donors varied from 1.46% to 3.36%, while the prevalence of HCV among blood donors ranged from 3.01% to 7.94%. Similarly according to six different studies from Karachi (between 2000- 2008), the prevalence of HBV among blood donors varied from 1.71% to 6.20%, while HCV prevalence ranged from 0.30% to 7.50%. Seven different reports from Peshawar (between 2000- 2011) indicated percentage prevalence of HBV, among blood donors, ranging from 1.40% to 2.54%; while HCV prevalence varying from 0.89% to 3.21%. Five different reports from Rawalpindi (between 2002- 2007) indicated HBV and HCV prevalence to be 2.16%-5.86% and 2.52%-6.21% respectively.

According to a World Bank report published in 2012, females are 49.19% (i.e. approximately 50%) of the total Pakistani population [21]. In Pakistani society, large proportion of females are confined to house settings and therefore, are comparatively less exposed to risk factors associated with HBV, HCV and HIV as compared to males. Our results show that among 160,376 individuals who donated blood, only 0.38% were females. Currently, an inadequate surveillance system for sexually transmitted infections exists with inadequate identification of subpopulations where deadly viral pathogens are secretly propagating. One of the possible ways towards harm reduction in Pakistan is by supporting community organizations and NGOs [59]. World Health Organization advocates voluntary non-remunerated blood donors (VNRBD) to achieve blood safety. Provision of safe and adequate blood is the responsibility of the state and steps have to be taken to promote VNRBD awareness campaigns in Pakistan. The process of blood safety system reform was initiated in 2008by the government with the support of the German government through the platform of Safe Blood Transfusion Program (SBTP).

The SBT Program has drafted the Blood Safety Act 2013, which will provide uniform, up to date and comprehensive blood safety framework throughout the country and has been endorsed by all Health Departments. The new act promotes the concept of VNRND and proposes a centralized model of blood transfusion services in which blood donations and screening will be a task of Regional Blood Centers and hospitals will not be required to collect blood.

To promote the culture of voluntary blood donations the SBTP and PIMS Blood Bank have lately been involved in developing close collaboration with the university student‘s community in Islamabad. This initiative involves the formation of a Network of university based Blood Donor Organizations (BDOs) and providing them technical guidance and support. The aim of this exercise will be to make Islamabad the first city in Pakistan to have complete reliance on voluntary blood donors with gradual phasing of the practice of forcing the family members and friends of the patients to provide blood donations. This model can then be applied to other cities in a step-wise manner. We hope this project envisages strategies to promote VNRBD and also work in other priority areas to achieve the ultimate objective of blood safety. Improvements in the donor management practices would lead to increase in VNRBD, blood safety and reduction in recurrent costs of a blood center.

Acknowledgement

Mr. Bilal Ahmed Tareen, Manager Blood Transfusion Services, Pakistan Institute of Medical Sciences (PIMS), Islamabad.

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