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Prevalence of Bovine Trypanosomosis in Didessa Woreda, Oromiya Region, Ethiopia
ISSN: 2157-7579

Journal of Veterinary Science & Technology
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Prevalence of Bovine Trypanosomosis in Didessa Woreda, Oromiya Region, Ethiopia

Netsa Bekele1, Abriham Kebede2* and Endalu Mulatu3
1College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia
2School of Veterinary Medicine, College of Medical and Health Science, Wollega University, Ethiopia
3College of Agriculture, Metu University, Bedelle, Ethiopia
*Corresponding Author: Abriham Kebede, School of Veterinary Medicine, College of Medical and Health Science, Wollega University, PO Box: 395, Ethiopia, Tel: +251-917-095-077, Email: [email protected]

Received Date: Nov 22, 2017 / Accepted Date: Jan 05, 2018 / Published Date: Jan 06, 2018

Abstract

A cross sectional study was conducted at Didessa Woreda, Oromiya region, south west Ethiopia. The study was carried out from November, 2011 to April 2012 on indigenous cattle breed managed under mixed corop-livestok production system, to determine the prevalence of bovine trypanosomosis. This study employs parasitological survey by the use of Buffy coat examination, thin smear and hematological study. The overall prevalence of bovine trypanosomosis in the present study was 5.47%. The predominant species recovered was Trypanosome congolense (61.9%) followed by Trypanosome vivax (23.8%) the Trypanasome brucei (9.5%). Mixed infection due to Trypanosome congolense and Trypanosome vivax (4.7%) was also recorded in the study. Discrepancy in the prevalence of Trypanosome infection was recorded in the different age groups, between sex and different body conditioned animals, but the difference was not statically siginificant (p>0.05). The mean PCV of parasitemic animals was significantly lower (21.15=4.675) than the parasitemic animals (24.316=4.93) (p<0.05). Although the present study came up with low prevalence of bovine trypanosomiasis in the stydy area, the potential impact of this disease on production and productivity of cattle shall not undermined. Therefore, sustainable community based tsetse and trypanosomsis control program should be implemented.

Keywords: Didessa; Ethiopia; Prevalence; Trypanosomiasis

Introduction

Trypanosomosis is disease cussed by unicellular parasites (trypanosome) found blood and other tissue of vertebrates; including live stock, wild life and people [1]. It is a serious disease in domestic live stock causing a significant negative impact on food production and economic growth in many parts of the world, particularly in subsaharan Africa [1-3]. Its epidemiology and impact on live stock production are largely determined by the prevalence, and distribution of the disease and its vectors in the affected area [4].

This disease is transmitted mainly by tsetse flies (cyclically), biting flies (mechanically) and by other means of transmission [3,5]. The most important species that infected cattle include Trypanosome congolense . T. brucei and T. vivax . Mechanically transmission is particularly important in relation to T. vivax and T. evansi particularly on the fringe of tsetse areas. It can occur in the presence of biting files of genus Tabanus. Haematopia, chrysopas and stomaxys [6].

Tsetse flies ingest Trypanosome in blood or lymph node while feeding on the host. The Trypanosome undergoes a cycle of development and multiplication in digestive tract of the fly until the infective metacyclic trypanosomes (metatrypanosome) are produced [3]. They undergo a transformation losing their typical Trypanosome or trypomastogote and metacyclic Traypanosomes which are infective froms of the host [7]. African trypanosomes lose infectivity for mammals when they enter the tsetse fly gut and must complete their development cycle with differentiation to the metacyclic stage before infective parasites can be transmitted in the tsetse saliva [8]. Approximately 30% of the total cattle population in African continent and about 50 million people are exposed to animal trypanosomosis and human sleep sickness respectively [9].

Tsetse flies (Glosina) inhabit wide range of habitats covering over 10 million km2 representing 37% of the African continent and affecting 37 countries including Ethiopia [10]. In Ethiopia tsetse flies are confined to the southern and western regions. Between the longitude 330 and 380 E and latitude 50 and 120 N. tsetse fly infested areas lies in the low land of and also in the river valley of Abay (Blue Nile), Baro, Akobo, Ghibe, Didessa and Omo [11]. Currently about 220,000 km2 area is infested with tsetse flies normally Glossina fuscipes, Glossina tachnoides, Glossina pallidipes, Glossina marsitans, Glossina Longipennis [12]. About 15-20 percent of the low land believed to be suitable for livestock production by one or two species of the tsetse flies [13].

Bovine trypanosomosis is a serious constraint to agricultural production in extensive tsetse infested areas of Ethiopia low land [14]. Trypanosomosis is prevalent in two main regions of Ethiopia i.e., the North West and the south west regions [6]. In Ethiopia, Trypanosomosis is one the most important disease limiting live stock productivity and agricultural development due to its high prevalence in the most arable and fertile land of south west part of the country following the grater basins of Abay, Omo, Ghibe, Didessa and Baroo with a high potential for agriculture [12].

The most important Trypanosome species affecting live stock in Ethiopia are T. congolense, T. vivax, T. brucei, in cattle, sheep and gaot, T. evansi in camel, and T. equiperdum in horse [6]. In Giemsa stained blood smears the species are distinguished by their size, shape, location, the size of kinetoplast, position of nucleus and the attachment and length of flagellum. Trypanosomes move actively and progress by movement of the undulating membrane and the free flagellum when present [3]. Trypanosomes are characteristically leaf like in shape; they are a single flagellum and attached to the organism by undulating membrane [15].

Animal caused by trypanosomosis is manifested anemia, generalized enlargement of superficial glands, loss of body condition, fever, and loss of appetite [7]. In recent years a number of drugs effective against cattle trypanosomosis have been introduced for both curative and prophylactic use [5,15]. Curative treatment is the most effective in herds that are inspected at regular intervals [3]. Control and strategies in the trypanosomosis concentrate on vector control, parasites control with chemotherapy and chemoprophylaxis and use their inherent trypano tolerant in some breeds of animals [6].

The economic burden of trypanosomosis is not only due to the direct losses resulting from mortality, morbidity and infertility of the infected animals but also it is due to the indirect losses like exclusion of live stock and animal; power based crop production from the huge fertile tsetse infested areas [16]. In Ethiopia about 5.5 million heads of cattle are exposed to the risk of trypanosomosis. This disease reduces meat and milk production of animals recovering from it. In addition to this some drugs are costly to treat animals that are diseased. Despite bovine trypanosomosis, have impacts on cattle production and prevalence of the disease in the study area.

Therefore the objective of this research was:

• To estimate the prevalence of bovine trypanosomosis in Didessa Woreda.

• To determine the species of trypanosomes that affect bovine species in study area.

• Determining PCV values in parasitemic and non-parasitemic animals.

Materials and Methods

A number of materials were required for both study of the disease and writing of this thesis. The list of the materials were sides, cover slips, PCV reader, pippet or stick, Bunsen burner, haematocrit clay, haematocrit centrifuge, microscope and equipment used for blood collection, (needle, needle holder, test tubes and haematocrit tube, the reagent used for laboratory test like iodine, methyl alcohol, giemsa stain, methyl blue stain and others.

Study area

The study area was located in oromia region, Illu Ababor Zone at Didessa woreda. Dembi is the administrative center of Didessa district. This district is bordered by Gechi Borecha distirict in north, Limmu Korsa in east, Sattama in west and Gumay in south and found at south west of Ethiopia at the distance of about 420 km from Addis Ababa. The topography of this district is characterized by plateaus of central and western plains of Didessa valley. The main river for this district is Didessa River and the tributaries Mulade, Asha and Dibo. Didessa is the smallest district in Ilu Ababor Zone with area of 615 km2. The elevation varies in this area from 1360-2340 meter above sea level. The annual mean temperature for most part of the district is 13°C-28°C and annual rain fall is about 900-1000 mm. the climatic condition of the area includes: Dega, woina Dega and kola cover 16%, 64%, and 20% of the district respectively. The lands used for cultivation are cultivated land 4719.57 hectar, grazing and 9848.45 hectare, forest 10682.7 hectare and the other 724 hectare [17].

Study population

Bovine with any age and either sex in Dinge Beraha and Busi Settkement area were selected for the collection of samples. There are a total number of 21,867 bovine species which is greater in number than other species of animals in that area [17]. A total of 384 cattle were selected with 192 from each peasant association. The cattle selected for sample collection were indigenous zebu cattle in Ethiopia.

Study design

A cross sectional type of study was conducted to determine the current prevalence of bovine trypanosomosis in the study area. The selected cattle were categorized according to their body condition (good, medium and poor), sex (female and male) and age (<year, 2-3 year and >3).

Sample size and sampling method

The simple random sampling technique was applied to collect from the ear vein. The sample size can be determined based on the study type and sampling method for investigation, 95% confidence interval, 5% desired absolute precision and 50% average prevalence and 38.4 cattle were sampled by the following formula [18].

N=(1.96)2 Pexp(1-Pexp)/D2

Where n=required sample size

Pexp=expected prevalence

D2=desired absolute precision

Study methodology

Buffy coat technique: A small blood was collected from an ear vein using heparinized microhaematocrit capillary tube. A haematocrit tube with a whole blood sample and end was sealed with haematocrit clay. The tube was centrifuged at 12000 revolutions per minute for five minutes. After centrifugation Trypanosome were usually found in or just above the buffy coat layer. The capillary tube was cut using a diamond tipped pen 1 mm bellow the Buffy coat to include the upper most layers of the red blood cells and 3 mm above to include the plasma. The content of capillary tube was expressed on to side, homogenized on to clean side and covered with cover slip. The slide was under x40 objective x10 eye piece for the movement of the parasites [19].

Thin blood smear: Blood drop of blood from a micro haematocrit capillary tube the side applied to clean side and spread by using another clean side at angle of 450, air dried and fixed for 2 minutes in methyl alchohol, then immersed in Giemsa stain (1:10 solution) for 50 minutes [20] drained and washed of excess stain using distilled water, allowed to dry by standing up right on the rock and was examined under microscope with oil immersion objective lens. In Giemsa stained smears the species were distinguished by their size, shape, location and size of the kinetoplast, position.

Measuring of packed cell volume: Blood samples were obtained by puncturing marginal car vein with lancet and collected directly in to a capillary tube. The capillary tubes were placed in micro haematocrit centrifuge with sealed end outer most. The tube was loaded symmetrically to ensuring good balance after screwing the rotators cover and closing the centrifuge lid, the specimens were allowed to centrifuge at 12,000 revolutions per minute for 5 minutes. Tubes were then placed in a haematocrit and readings were expressed as a percentage of packed cells to the total volume of whole blood. Animals with PCV<24% were considered to be anemic.

Body condition scoring: The body condition was characterized good, medium and poor according to Nicholson and Butter worth [21].

Data management and analysis: Row data individual animals and parasitological examination were inserted in to micro soft excel spread sheets to create a data base and transferred to SPSS version 17.0 software program for data analysis.

Results

Out of the total 364 cattle examined 21(5.47%) cattle were positive for trypanosomosis, the prevalence of trypanosomosis were not statistically siginificant (P>0.05) between different sex (P=0.611), age (p=0.676) or body condition score (p=0.4) of the animals as indicated in Tables 1-3 respectively. From the total infected animals 13 (61.9%), 5 (23.8%), 2(9.5%) and 1(4.76%) were infected with T. congolense, T. vivax and with mixed infection of T. congolense and T. vivax respectively (Table 4).

Sex Frequency Percent Trypanosome infected Rate of infection (%) P- value
Female 203 52.9 10 4.9 0.611
Male 181 47.1 11 6.07  
Total 384 100 21    

Table 1: Prevalence of bovine Trypanosome with sex in Didessa woreda.

Age Frequency Percent Trypanosome infected Prevalence P – value
(<1) 8 2.1 0 0  
(2-3) 92 24 5 5.43 0.67
>3 284 74 16 5.63  
Total 384 100 21    

Table 2: Prevalence of bovine Trypanosome with age in Didessa woreda.

Body score Frequency Numbers of animal infected with trypanosome Prevalence P-value
Good 69 3 3(4.30 0.04
Medium 150 6 6(4)  
Poor 165 12 12(7.27)  

Table 3: Prevalence of bovine Trypanosome with Body condition in Didessa woreda.

Species Total Frequency Percent
T. brucei 21 2 9.52
T. congolense 21 13 61.9
T. vivax 21 5 23.8
Mixed (T. congolense and T. vivax) 21 1 4.76

Table 4: Prevalence of bovine Trypanosome species in Didessa woreda.

The mean PCV of the animals infected with trypanosomes (21.857 ≠ 4.67) was significantly lower (p<0.032) than the average PCV of the animals that were parasitologically negative was (24.316 ≠ 4.93) (Table 5).

Condition No. Examined (PCV<25%) (PCV>25%) Mean pcv%+sd p-value
Parasitemic 21 (5.47%) 17 (80.95%) 4 (19.05%) 21.857 ≠ 4.675 0.03
Aparasitmeic 363 (84.5%) 173 (47.66%) 190 (52.34%) 24.316 ≠ 4.93  
Total 384 190 (49.48%) 194 (50.52%)    

Table 5: Mean packed cell volume and standard deviation of infected and non-infected animal cattle in Didessa woreda.

Discussion

The result of the present study revealed an overall trypanosomosis of 5.45%. This finding was lower than the previously reported prevalence rate of 23% in western Ethiopia, 21% in Metekal district, 18.5% in Arba-minchi district, 17.5% in upper Dideesa valley areas, and 11.7% in Abay Basin north western Ethiopia of tsetse infested regions [22-26]. The lower prevalence in the current study might due to the low sensitivity of the parasitological diagnostic method, the uncontrolled use of trypanocidal drugs, application of relatively well designed method of tsetse control and treatment, expansion of cultivation in the area which in directly affects flies distribution and awareness of the people towards the control and treatment of the disease were improved. Even though, the data was not collected during rainy season, [27] revealed that there is significantly high infection rate following the months with high rain due the emergency of biting flies t high rate. This implies that the low prevalence of trypnosomosis in this study may be related with decrease in fly population during dry season.

The finding of this revealed that the majority of the infection was due to T. congolense (61.9%) followed by T. vivax (23.8%) and T. bruecei (9.5%). Mixed infection of T. congolense and T. vivax was also prevalent (4.76%). The higher proportion of T. cogolense infection study area was in agreement with Trypanosome species prevalence data from other tsetse infested regions of Ethiopia, where the T. congolense is the most prevalent species in cattle [28]. The ratio of T. congolense, T. vivax and T. brucei was 2.6: 1:0.4 indicating high Trypanosome infection due to T. conogolense. The percentage specious distribution in our finding was similar to Nigatu his co-workers finding in Abay Basin (T. congolense (66.1%) followed by T. vivax (20.8%) [24]. The predominant species of T. congolense compared to T. vivax and the development of better immune response to T. vivax infected animals [29,30].

The prevalence of infection between sex categories was 6.07% in male and 4.9% in female animals. However, there was no significance difference between sex groups (p=0.611).

There no significance difference observed in age groups in the study groups in the study period but relatively higher rates observed in adult (>3 years) animals when compared to young animals. This may due to exposition adult animals for the tsetse fly [31]. When they are freely grazing and also may be due to immune suppression as a result of stress factors such as lactation and when they are travelling along distance through tsetse challenges are for drafting purpose in males. The low prevalence in young animals may also be due to the natural protection to some extent by maternal antibodies [32].

Although higher infection rate was observed in poor body conditioned animals as compared to good and medium, in the present study no statistically different was observed this groups (p=0.4). Similar results were reported from Tselemty woreda, Western Tigtay, Northern Ethiopia [33]. PCV is the most reliable indicator of anemia in trypanosomosis [34,35]. In our study Trypanosome infection results in a significant decline in PCV, this is in agreement with previous finding that are reported by different authors at different time [36-37].

The mean PCV values of studied animals was significantly (p<0.05) varying between parasitemic (21.857 ≠ 4.857%) and a parasitaemic (24.316 ≠ 4.93%). This result was in agreement with the previous result by Sinishaw et al. [27]. The appearance of parasitologically negative animals with PCV values of less than the threshold values (25%) may be due to the inadequacy of detection method used or delayed recovery of anemic situation after current treatment with trypanocidal drugs; and the occurrence of positive animals with PCV of greater than 25% might it be thought of recent infection. Trypanosome infection and mean values obtained in this study in the parasitaemic animals was found to be highly associated. Similar results were also reported by different authors in southern, north western and south western Ethiopia [24,30,38]. It was generally accepted that the mean PCV in affected by many factors (helmenthiasis, tick born disease and Nutritional imbalance) other than trypanosomosis. However, these factors are likely to affect both trypanosomosis positive and negative animals [39].

Conclusion and Recommendation

The overall prevalence of bovine Trypanosome infection is study area was lower than the previous report but it is important disease that affects the health as well as productivity of cattle in Didessa district. There was no statically significant difference between sex, ages, and body condition. The mean PCV of parasitemic animals due to T. Congolese, T. vivax, and T. brucei and mixed was significantly lower than a parasitemic animal.

Although, the present study low prevalence of bovine trypanosomosis in the study area potential impact of T. congolense, T. vivax, T. burucei infection on production and productivity of cattle shall not be undermined. Based above conclusion the following recommendations are recommended:

• Sustainable community based tsetse and trypanosomosis control program should be implemented.

• Major concern should be given to the identification of trypanosomes species with the highest prevalence and control measure should be targeted accordingly.

• Training and advising the society how to control the vector of the disease should be practiced.

• Use of the trypano-tolerant breeds of animals in the highly prevalent area should also be recommended.

Competing Interest

The authors declare that they have no competing interest.

Acknowledgement

We would also like to express our gratitude to Jigjiga University and people of Didessa district for supporting us to conduct this research.

References

Citation: Bekele N, Kebede A, Mulatu E (2018) Prevalence of Bovine Trypanosomosis in Didessa Woreda, Oromiya Region, Ethiopia. J Vet Sci Technol 9: 503. DOI: 10.4172/2157-7579.1000503

Copyright: © 2018 Bekele N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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