Reach Us +44-1647-403003
Prevalence of Primary Pulmonary Multi-Drug Resistant Tuberculosis in and around Aligarh Region
ISSN: 2161-0703

Journal of Medical Microbiology & Diagnosis
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Prevalence of Primary Pulmonary Multi-Drug Resistant Tuberculosis in and around Aligarh Region

Maryam Faridi1*, Indu Shukla1, Nazish Fatima1, Sumit Varshney1 and Mohammad Shameem2
1Department of Microbiology, J.N. Medical College AMU, Aligarh, Uttar Pradesh, India
2Department of TB and Respiratory Diseases, J.N. Medical College AMU, Aligarh, Uttar Pradesh, India
*Corresponding Author: Dr. Maryam Faridi, Department of Microbiology, J.N. Medical College AMU, Aligarh, Uttar Pradesh, India, Tel: 0571 522 8770, Email: [email protected]

Received Date: Sep 18, 2018 / Accepted Date: Oct 22, 2018 / Published Date: Nov 05, 2018


Tuberculosis (TB) is one of the most ancient diseases of mankind and has co-evolved with humans for many thousands of years or perhaps for several million years. M. tuberculosis strains that are resistant to the two most potent anti-TB drugs Isoniazid and Rifampicin, are termed as multidrug-resistant TB (MDR-TB) strains. Drug resistance is broadly classified as primary and acquired. Drug resistance in a patient who has never received anti-TB treatment previously or has taken treatment for less than a month is termed as primary resistance. Acquired resistance is the resistance which arises as a result of specific previous treatment. This study was aimed to determine the prevalence of primary MDR-TB in and around Aligarh region by molecular diagnostic method of Line probe assay (LPA). This two year study was carried out in culture and DST Laboratory (RNTCP certified), Department of Microbiology, J.N. Medical College AMU, Aligarh on the sputum samples received of the primary pulmonary tuberculosis suspected patients (according to PMDT guidelines) from the outpatient and inpatient departments of the hospital and from various tuberculosis units in and around Aligarh region from October 2015 to October 2017. Sputum samples were collected from suspected cases of primary pulmonary TB. These samples were subjected to routine microscopy and culture on LJ medium to detect Mycobacterium tuberculosis. Positive cases were subjected to drug sensitivity test by GenoType MTBDRplus Assay. Out of the total 514 samples collected, 265 (51.56%) samples and 326 (63.43%) samples were positive by ZN microscopy and fluorescent microscopy respectively. 312 (60.70%) samples were positive on culture on LJ medium. Total 326 samples which were positive on fluorescent microscopy were subjected to LPA and 47 (9.14%) samples were resistant to both rifampicin and isoniazid, 21 (4.08%) samples were rifampicin mono-resistant and 31 (6.03%) samples were isoniazid mono-resistant.

Keywords: Tuberculosis; Drug resistance; Microscopy 


Tuberculosis is an infectious bacterial disease caused by Mycobacterium tuberculosis which most commonly affects the lungs. It is transmitted from person to person via droplets from the throat and lungs of people with active pulmonary disease [1]. TB is a serious global public health threat. TB is the ninth leading cause of death worldwide and the leading cause from a single infectious agent, ranking above HIV/AIDS [2]. In 2016, there were an estimated 1.3 million TB deaths among HIV negative and an additional 374 000 deaths among HIVpositive people [2]. Globally in 2016, an estimated 4.1% of new cases and 19% of previously treated cases had Multidrug resistant TB [2]. Rapid identification is important for effective treatment and control of MDR-TB. Conventional methods of drug susceptibility testing (DST) include solid media-based methods such as the proportion, absolute concentration, and resistance ratio methods. These can take up to 12 weeks to produce definitive results, leading to prolonged infectiousness [3]. Liquid media-based tests are more rapid, but also costlier and require sophisticated laboratories and trained personnel [3]. Molecular LPA permit rapid diagnosis of TB, isoniazid and rifampin resistance, and clinically relevant non-M. tuberculosis mycobacteria. In LPA assays, DNA or RNA is isolated from culture or direct (i.e., sputum) respiratory samples and then amplified and reverse hybridized onto a nitrocellulose strip with immobilized probes for different mycobacteria or for mutations that confer resistance. These strips can be quickly interpreted using a template, with the entire testing process taking a day or even less. The GenoType MTBDRplus (Hain Lifesciences, Nehren, Germany) identifies rifampin and isoniazid resistance by detecting the most common mutations of the rpoB gene and the katG and inhA genes, respectively.

Materials and Methods

Sputum samples from 514 (n=514) suspected new pulmonary TB patients were collected and subjected to ZN microscopy and fluorescent microscopy and cultured on LJ media. Sputum positive samples were tested by LPA for the presence of M. tuberculosis complex and resistance to isoniazid and rifampicin.


The GenoType MTBDRplus LPA was performed according to the manufacturer’s (Hain Life-science, Nehren, Germany) instructions. Three steps for LPA test include DNA extraction, multiplex polymerase chain reaction (PCR) amplification and reverse hybridization. These steps were carried out in three separate rooms with restricted access and unidirectional workflow. LPA strips were observed and read for the presence of TUB band, amplification control band and conjugation control band and absence of any wild type (WT) band or presence of any mutation (MUT) band. The results were then interpreted as sensitive or resistant to any particular drug.


Out of 514 (n=514) sputum samples 256 (51.56%) samples were positive by ZN microscopy and 326 (63.43%) samples were positive by fluorescent microscopy. 312 (60.70%) samples were culture positive on L J media (Table 1 and Figure 1).

Figure 1: Comparison of ZN smear examination, fluorescent microscopy and mycobacterial culture on LJ medium of MDR-TB suspected patients.

Total no. of patients ZN smear positive no. (%) Fluorescent microscopy positive no. (%) Mycobacterial culture positive no. (%)
514 265 (51.56%) 326 (63.43%) 312 (60.70%)

Table 1: Comparison of ZN smear examination, fluorescent microscopy and mycobacterial culture on LJ medium of MDR-TB suspected patients (n=514).

Out of total 326 samples subjected to LPA, 6 samples were TUB band negative and 320 samples were TUB band positive (Table 2 and Figure 2).


Figure 2: LPA result.

Tub Band Positive Tub Band Negative Total LPA
320 (98.16%) 6 (1.84%) 326 (100%)

Table 2: TUB band of the samples.

The anti-mycobacterial sensitivity profile observed on LPA was such that 21 (4.08%) samples were mono-resistant to rifampicin, 31 (6.03%) samples were mono-resistant to isoniazid and 47 (9.14%) samples were observed to be resistant to both isoniazid and rifampicin (Table 3 and Figure 3).


Figure 3: Anti-mycobacterial sensitivity profile of primary pulmonary tuberculosis suspected patients as detected by LPA.

No. of patients MDR (R+H) (%) Mono-resistant R (%) Mono-resistant H (%) Sensitive (R+H) (%) Total (%)
514 47 (9.14%) 21 (4.08%) 31 (6.03%) 221 (42.30%) 514 (100%)

Table 3: Anti-mycobacterial sensitivity profile of primary pulmonary tuberculosis suspected patients as detected by LPA (n=514).


In our study out of the total 514 samples, 265 (51.56%) were AFB positive and 249 (48.44%) were smear negative. In a similar study by Tripathy et al. reported 47.65% cases were smear positive for AFB which is comparable to our study [4]. Mukherjee et al. in their study found 38.64% samples positive by ZN staining [5].

The present study showed that out of total 514 samples, 326 (63.43%) samples were positive for fluorescent microscopy and 188 (36.57%) were negative. The study clearly indicated that the case detection rate of fluorescent microscopy is remarkably higher than that of ZN microscopy. The other advantages of less eye strain, easy visualization, less time consumption and even detection of low number of bacteria in comparison to ZN method cannot be overlooked also.

In the present study, out of 514 samples 312 (60.70%) samples were positive for culture on LJ media and 183 (35.60%) samples were negative however, 19 (3.70%) samples were dry/contaminated. Kelamine et al. in their study also faced contamination rate of 3% [6]. Singh et al. reported 4.9% contamination in their study [7].

In this study positivity rate of ZN microscopy, fluorescent microscopy and mycobacterial culture positive were 265 (53.53%), 326 (65.85%) and 312 (63.03%) respectively. In a similar study by Laifangbam et al. 44.1%, 71.6% and 70% cases were found positive by ZN, fluorescent microscopy and culture respectively, which is comparable to the data achieved in our study [8]. In another study by Laifangbam et al. positivity rates for ZN microscopy, fluorescent microscopy, and culture were 36.1%, 74.1%, and 72.2%, respectively which is again close to the data of our study however positivity rate of ZN microscopy in our study is quite high [8].

In our study 514 samples were subjected to microscopy, out of 514 samples 326 fluorescent microscopy positive samples were further subjected to LPA. TUB bands were present in 320 (98.16%) samples out of the total 326 samples subjected to LPA and TUB bands were absent in 6 (1.84%) samples. In the present study, on the basis of LPA 47 (9.14%) samples were found to be resistant to both rifampicin and isoniazid, mono-resistant to rifampicin were 21 (4.08%) samples, mono-resistant to isoniazid were 31 (6.03%) samples and 221 samples were sensitive to both isoniazid and rifampicin. Different observations of rates of MDR have been made by LPA at different times. 3.5% of primary MDR-TB was documented by Global TB report 2014 [9]. In 2015 MDR-TB was reported 3.9% in Global TB report [10]. Global TB report 2017 has documented primary MDR at 4.1% [2]. The prevalence of MDR-TB varies between 0.07 to 5.7% across India [11-17]. A study by Desikan et al. showed their 19.1% isolates were MDR, 10.6% were rifampicin mono-resistant, 8.3% were isoniazid mono-resistant and 61.9% strains were found to be pan-sensitive [18]. A similar study by Almeida et al. showed MDR in 30% of new cases [19]. Mathuria et al. in their study reported primary drug resistance of 13.3% in Varanasi, Uttar Pradesh; 7.1% in Sawaimadhopur, Rajasthan; 25% in Buxar, Bihar [20] Gaude et al. reported 11.1% primary drug resistance in their study in Kranataka [21]. Jain et al. reported primary drug resistance of 13.2% in a study in Lucknow [22]. In a similar study by Agwan et al. in Meerut primary MDR came out to be 7.2% which is quite comaparable to our data and they showed, 17.3% rifampicin mono-resistant and 4.5% isoniazid mono-resistant [23].


In this study the prevalence of primary pulmonary multidrug resistant tuberculosis was found to be 9.14% and isoniazid monoresistance was found to be 6.03% and rifampicin monoresistance was reported as 4.08%. Keeping in mind the socio-economic status of the patients seeking medical service in the institute, this much high prevalence of drug resistant TB can be justified however, factor that lead to clustering of data like we were primarily concerned with the TB patients in the institute itself cannot be overlooked.


Citation: Faridi M, Shukla I, Fatima N, Varshney S, Shameem M (2018) Prevalence of Primary Pulmonary Multi-Drug Resistant Tuberculosis in and around Aligarh Region. J Med Microb Diagn 7: 285. DOI: 10.4172/2161-0703.1000285

Copyright: © 2018 Faridi M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Select your language of interest to view the total content in your interested language

Post Your Comment Citation
Share This Article
Recommended Conferences
Article Usage
  • Total views: 2644
  • [From(publication date): 0-0 - Mar 31, 2020]
  • Breakdown by view type
  • HTML page views: 2499
  • PDF downloads: 145