Department of Chemistry, College of Natural Science, Arbaminch University, Arbaminch, Ethiopia
Received Date: September 04, 2015; Accepted Date: September 05, 2015; Published Date: September 12, 2015
Citation: Fenta AD, Kidanemariam AA (2015) Profile of Essential and Non- Essential Metals in Soil and in Khat (Catha Edulis Forsk) Leaves Cultivated in Southern Region, Ethiopia. Chem Sci J 6:107. doi:10.4172/2150-3494.1000107
Copyright: © 2015 Fenta AD, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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This study was conducted with the objective of determining the quantity of selected essential and nonessential metals; Co, Mg, Ca, Cu, Mn, Cr, Cd, Fe, Zn and K in the leaf and supporting soil of Khat (Catha edulis Forsk). Samples of three Khat types (Konso, Gidole and Koyra) and soils from their root zone collected from three different sites in southern Ethiopia region were analyzed by flame atomic absorption spectrometry. K in soil and Khat sample were also determined by flame atomic emission spectroscopy. Known weight of oven-dried Khat samples were wetdigested using 2 mL of (69-72%) HNO3 and 2 mL of (70%) HClO4 for 130 min at variable temperatures (120-268°C). Soil was digested by the procedure, 0.5 g samples of soil were digested with concentrated nitric acid, concentrated hydrochloric acid and hydrogen peroxide, using Kjeldahl digestion block under reflux condenser for 3 h at 300°C. Both the edible portion of Khat leaves and the soils of the study farms showed similar accumulation patterns to some extent, in their contents of the studied macro and micronutrients. Regression analysis and pearson correlation (r) results show a positive correlation Coffecient (R2) values ranging from 0.281 up to 0.991 and for pearson correlation (r) values ranging from 0.244-0.951. Although regressions based on the pooled data from the three Khat types are not adequately correlated with total metal soil levels, better fits were obtained when regression models were used for Konso Khat separately. However, no strong correlations between the leaves and other variables are evident except for Ca and Mg metal contents which have r values of 0.971 and 0.991 respectively with (p<0.01) levels. The concentrations of the metals were also compared with recommended maximum permissible limits and some international reports.
Fresh leaves of Khat (Catha edulis Forsk) are customarily chewed by the inhabitants of East Africa and Southern Arabia to attain a state of stimulation. Its young leaves and stem tips contains higher proportions of cathine and cathinone, which are responsible for much of the stimulant effect of Khat. Khat chewing is highly prevalent in East African and some Middle Eastern countries. Its use is both a social and a culture-based activity and it is said to enhance social interaction. On average, almost 70% of households in Yemen and 50% in Djibouti use Khat . It was reported in a Somalia-based study that approximately 36.4% of people had chewed Khat and more than 30% of Ethiopians have been reported to use Khat . The overall prevalence of Khat chewing in various regions of Ethiopia is well documented; among upper secondary school students 64.9%, in a rural community in southern Ethiopia 50%, at a university located northern Ethiopia, Axum 28.7%, and in the Eastern Ethiopia 42.2% [3-5].
Studies on chemical composition of plant materials are growing as a result of ongoing developments in agriculture, nutrition, environmental studies, biogeochemical surveying and mineral prospects. All these developments have led to increased demands for analysis of complicated biological matrices. Various reports have discussed the potential health implications of trace metals in various plants and fruits, Cd, Pb, Ni and Al, hereinafter referred to as toxic heavy metals and may pose a serious threat to plant, animal, human and environmental health, because they are not degradable by bioprocess and remain in environment and passes to food chain. It is globally accepted that some heavy metals such as Fe, Zn, Cu, and Mn; hereinafter referred to as micro-elements; are essential for healthy growth and development within certain permissible limits. Based on the effect of heavy metals on consumers, different organizations have proposed maximum permissible limits of the metals in edible vegetation, wastewater, and soils [6-8].
In addition to plant materials, the analysis of soil is an important diagnostic tool for identifying nutrient deficiency or excess. Soil analysis has the advantage over the plant analysis as well as visual deficiency symptoms in indicating the extent of nutrient deficiency or their requirement for crops prior to seeding. The identification of macro and micro constituent, as well as contaminants and the establishment of hazards levels are only possible if the soil composition is known by using reliable and comparable chemical measurements . Further, air, food and water pollution is widely anticipated and reported in industrialized and densely populated areas. In countries like Ethiopia, there is not enough industry to cause substantial pollution. The major source of contamination comes from agricultural activities, such as the application of fertilizers and pesticides. Hence, an increasingly important aspect of food quality should be to control the concentrations of trace metals in food.
Now days, important progress has been made in understanding the pharmacological and social effects of Khat. However, less attention has been paid to the chemical profile in Khat and their role in distributing trace element levels in human tissues and body fluids.
Few papers has been reported on essential and non-essential metals of Ethiopian Khat more specifically [2-4,7]. However, correlations of elemental concentrations of Khat with its supporting soil are scarce in the literature. As this issue has not been much studied in Ethiopia the main aim of this study was to assess the profile of selected essential and nonessential metals in Khat leaves cultivated in southern region, Ethiopia with its supporting soil.
Reagents and chemicals
An acid mixture of 1:1 conc. HNO3 (69% LR, Breckland Scientific Supplies, UK) and conc. HClO4 (69.0-72%, Blulux, Laboratory reagent) was used for digestion Khat samples. Concentrated H2SO4 (98%, laboratory reagent, LOBA, India) and Selenium powder (99%, Breckland Scientific Supplies, UK) were used in the optimization for Khat digestion process. A solution of LaCl3•7H2O (99.9%, Blulux, Laboratory Pvt. Ltd) was used in the determination of Ca and Mg. (NH4)2Fe(SO4)2•6H2O (99%) and K2Cr2O7 (99.5%) (Blulux, Laboratory reagent), conc. Orthophosphoric acid (85-88%, Blulux, Laboratory reagent), and bariumdiphenyl amine sulphonate (BDH chemicals Ltd, England) solutions were used to determine soil organic matter. HCl, NaOH, CaCO3 powder and phenolphthaline indicator solution were used for the determination of CaCO3 percent content of the soil. KCl (99.5%, Analytical reagent, CDH (P) LTD, India) was used for soil acidity determination.
A water deionizer (Elgalan Instrument, UK) was used to produce deionized water. Digital analytical balance (Explorer, Ohaus, Model E11140, Switzerland) with ± 0.0001 g precision were used for all measurements of samples. The samples of Khat and soil were digested in a digestion tube using Kjeldahl digestion block. Atomic flame absorption spectrophotometer (Model 210/211 VGP Buck Scientific AAS VER 3.74) was used to determine metal concentration in the samples. Flame atomic emission spectroscopy (Ciba Corning Diagnostics Scientific Instruments M410, UK) was used to determine K metal concentration in the aforementioned samples. Digital pH meter (ELE International PQ qualab) was used to determine the pH of soil samples after stirring by a magnetic stirrer (Hanna instruments, model H1200, UK).
Description of the study area
Gamo gofa zone is a zone in the Ethiopian southern nations, nationalities, and peoples region (SNNPR). It is found at 6.2500° N latitude and 37.0000° East Longitude. It covers a total surface area of 12581.4 square kilometer. Gamo gofa is bordered with on the southwest by debub (South) Omo and the Basketo special woreda, on the northwest by Konta special woreda, on the north by Dawro and Wolayita, on the northeast by the Lake Abaya which separates it from the Oromia Region, and on the southeast by the Amaro special woreda. The administrative center of Gamo Gofa is Arba Minch. A map and detail of the study area is given in Figure 1. Oil crops, cotton, cattle fattening, dairy farm, sheep and goat fattening, apiculture, ostrich farm are common agricultural practices exercised in the area.
Collection and analysis of samples
Samples of Khat leaves and supporting soils were gathered from different sites of Gamo Gofa zone of Southern region of Ethiopia which are well known in producing, marketing Khat. Samples of different Khat types and its supporting soil from farms of local farmers were collected in Konso, Gidole and Koyra Sites. The three varieties of Khats were distinguished by the local farmers in the area based on their narcotic effect, exciting rate and colors. In order to avoid any confounding differences in the environmental factors-such as type of soil and fertilizers, irrigation system and humidity-that may affect the chemical composition of the Khat leaves, the samples were collected from different places of each region and pooled together. The upper recently mature leaves free of physical damage and injury from insects and of fungal infestation were selected for this study. The Soil samples were collected from surface soils close to the each Khat varieties (0-30 cm depth) and brought to the laboratory wrapped in polyethylene bags. In the laboratory, the Khat leaves were washed with deionised water. The leaves were dried at room temperature. The dried samples of Khat and soil were then separately grinded to fine size using two sequential millers; Mill one (2.00 mm mesh size) and Mill two (1.00 mm mesh size). The powders were subsequently stored in clean polyethylene bags until digestion. To avoid cross contamination, the stainless steel grinding system of the millers were washed with acetone after or before each grinding cycle.
Optimization of digestion procedure
To select an optimum procedure for digestion, parameters like digestion time, reagent volume, volume ratio of reagents, and digestion temperature were optimized by varying one parameter at a time and keeping the others constant. Parameters giving clear solution at lower temperature, requiring minimum reagent volume and digestion time were selected as an optimum procedure for digestion of Khat and soil samples.
Digestion of Khat samples
The samples were digested following the procedure recommended by the AOAC, 1990. About 1.0 g of ground and meshed Khat leaf samples in a clean dish were dried over night at 105°C in an oven and then cooled in desiccator. 0.5 g portions were weighed and transferred into digestion tubes. 4 mL of conc.1:1 HNO3 and HClO4 were added and swirled carefully to moisten the Khat sample and left to stand for 2 hr. The digestion tubes were placed in a Kjeldahil block and heated between 120-268°C for about 130 min in variable temperature. The tubes were removed and cooled to room temperature. To this, 45 ml of deionized water was added and mixed thoroughly. This solution was left standing to cool at room temperature, mix again, filtrated on a 100 ml volumetric flask, bringing to volume with distilled-deionized water and stored in a plastic bottles for the determination of metals. Triplicate digestions were carried out for each bulk sample.
The samples were analyzed after digestion for their metal contents of Ca, Fe, Mn, Cu, K, Zn, Co, Cr, Mg, Pb and Cd by the Flame Atomic Absorption/Emission Spectrometer after an external calibration using aqueous calibration standards prepared from stock standard solutions of the respective elements in absorbance mode. Where, K in the sample digest was determined by emission mode flame photometer in order to avoid the ionization interference. Further, for Ca and Mg metals, excess of lanthanum was added to avoid the formation of refractory compounds that would depress the signal.
Digestion of soil samples
A digestion method reported by ref.  was used for the digestion of the soil samples after making a slight modification on the procedure to obtain clear solutions of the digest. 1.0 g of soil was accurately weighted and transferred into 100 mL round bottom flask and moistened with 1 mL of distilled deionized water 10 mL of 1:3 mixtures of conc. HNO3 and HCl was added to the flask and kept for hours until it got stabilized. Then, digested on Kjeldahl digestion block under reflux condenser for 2 hr at 140°C. The digest was left to stand for 30 min to cool down to room temperature, then about 50 mL of distilled deionized water was added to flask, filtered through Whatman No. 1 filter paper in to 100 mL volumetric flask and made up to the mark with rinsing the digestion flask. The solutions were used for the analysis of the total soil metal concentrations for Ca, Fe, Mn, Cu, K, Zn, Co, Cr, Mg, Pb and Cd by the Flame Atomic Absorption/Emission Spectrometer. The concentration of K was determined in emission mode of the spectrophotometer. Furthermore, CaCO3 Content, organic matter content and pH of the soil samples were analyzed in a suspension of a 1:1 soil: water mixture.
Method detection limit
To determine method detection limit, replicate analyses for all blank samples were performed, and the pooled standard deviation of the seven reagent blanks was calculated. The detection limits were obtained by multiplying the pooled standard deviation of the reagent blank by three. The results clearly show that the calibration curves with good correlation coefficients and lower method detection limits were obtained during the analysis.
Evaluation of analytical method
The efficiency of the optimized procedure was evaluated using recovery experiment, i.e., by the procedure as follow: 0.5 mg of K, 0.1 mg of Ca, 0.1 mg of Mg which contains a total of 1.5 mL solution were spiked at once into a first digestion tube which has known weight Khat leaf sample and 0.5 mg of Fe, 0.5 mg of Mn, 0.5 mg of Cu which has a total volume of 1.8 mL were spiked into a second digestion tube containing the same Khat sample. 0.75 mg of Zn and 0.2 mg of Cd were spiked in to a third digestion tube. The rest for 0.1 mg of Pb , 0.1 mg Cr and 0.5 mg Co were spiked into a fourth digestion tube. Each sample was determined for their respective spiked metals. Recovery test was also performed for soil samples using the same procedure. Each recovery test for both samples was performed in triplicate. The percentage recoveries of each sample are within the acceptable range.
Due to the absence of certified reference material for leaves and soil sample in the laboratory, the efficiency of the optimized procedure was checked by adding known concentration of each metal in Khat leaves and supporting soil samples. The spiked and non-spiked samples were digested and analyzed in similar condition. Then the percentage recovery of the analyte was calculated by ref. :
Where, CM = concentration of metal of interest.
As shown in Table 1, the results of percentage recoveries for the studied metal nutrients in Khat leaves and supporting soil were all between 91 to 108% and 90 to 110% respectively except for recovery percent of Ca which gives a value of 112% in the leave. Therefore, this verifies that the optimized digestion procedure was valid (good accuracy) for Khat leaves and soil sample analysis.
|Elements||Conc. in the sample (mg/kg)||Amount added (mg)||Concentration in thespiked sample||Amount recovered (mg)||Recovery (%)|
|Ca||2.683 ± 0.02mg/g||0.1||2.795 ± 0.02 mg/g||0.112||112 ± 7.10|
|Fe||741.67 ± 0.13||0.5||742.21 ± 0.23||0.54||108 ± 2.00|
|Mg||3.98 ± 0.09mg/g||0.1||4.079 ± 0.12 mg/g||0.099||99 ± 5.21|
|Cr||7.5 ± 0.5||0.1||7.5097 ± 1.20||0.097||97 ± 4.00|
|K||6.99 ± 0.08 mg/g||0.5||7.47 ± 0.04||0.48||96 ± 2.00|
|Cd||2.12 ± 0.02||0.2||2.30 ± 0.01 mg/g||0.189||94.5 ± 3.00|
|Zn||167.21 ± 3.81||0.75||167.9 ± 6.50||0.699||93.2 ± 6.11|
|Pb||ND||0.1||0.09 ± 0.003 mg/g||0.092||92 ± 2.20|
|Mn||36.02 ± 0.29||0.5||36.5 ± 0.40||0.46||92 ± 1.01|
|Cu||19.23 ± 1.10||0.2||19.41 ± 0.89||0.182||91 ± 2.40|
|Co||1.57 ± 0.07||0.1||2.49 ± 0.21||0.456||91.78 ± 0.81|
Table 1: Recovery test for the optimized procedure of Khat leave sample.
Levels of selected essential and non-essential metals in Khat leaf samples
In the present study the concentration of nine essential (K, Ca, Mg, Mn, Fe, Cu, Cr, Zn and Co) and two non-essential (Pb and Cd) metals in Khat leave samples of the three varieties (Konso, Gidole and Koyra) were quantified.
As can be seen from the data in Table 2, there is a wide variation in concentration of macro and micronutrients within and among the Khat varieties. The most abundant metals among the macro elements was K followed by Mg and Ca whereas Fe contents of the Khat leaves was the predominant among the tested micronutrient heavy metals followed by Zn, Mn, Cu and Cr. It can be deduced from the levels of all the metals in the studied Khat types of all varieties, that the concentrations of the macro and micro nutrient metals followed similar trend for all the sample sites. In ref. [3,12] has reported the ranges for essencial and nonessencial metals of different Ethiopian Khat. In addition, ref.  has reported the nutrient concentrations in the Kenyan Khat leaves. The pattern of concentration of elements in most of Khat varieties obtained by ref. , were in the order: Ca>Mg>Fe>Mn>Zn>Cu>Cr>Co, while Cd and Pb were below the method detection limit of his analysis ref.  has also reported that the macro nutrient metal concentrations ranges; K (10.3-30.3 mg/g), Ca (9.44-33.2 mg/g) and Mg (1.14-7.19 mg/g). The higher level of K in the studied Khat plants according to aforementioned people were due to the fact that nutrient elements such as N, P, K, S and Mg are highly mobile in plant tissue and translocated from old leaves to young leaves. It is also reported that a higher concentration of Ca and Mg in Khat samples and suggested that, this is due to a broad range of Ca-bearing minerals in soil and water which are usually abundant and can easily be absorbed by the plant.
|Ca(mg/g)||3.00 ± 0.12||2.81 ± 0.03||3.12 ± 0.02|
|Fe(mg/kg)||676.67 ± 3.65||573.33 ± 5.62||741.67 ± 7.12|
|Mg(mg/g)||4.530 ± 0.11||3.980 ± 0.09||4.576 ± 0.01|
|Cr(mg/kg)||4.09 ± 0.29||2.75 ± 0.32||1.52 ± 0.13|
|K(mg/g)||15.37 ± 0.48||8.73 ± 0.04||13.79 ± 0.32|
|Cd(mg/kg)||2.12 ± 0.02||*ND||*ND|
|Zn(mg/kg)||136.423 ± 1.81||167.21 ± 3.80||202.64 ± 2.21|
|Mn(mg/kg)||85.29 ± 1.36||36.02 ± 0.29||27.206 ± 0.31|
|Cu(mg/kg)||16.9 ± 1.00||22.5 ± 1.41||20.19 ± 0.53|
|Co(mg/kg)||1.57 ± 0.07||0.324 ± 0.05||*ND|
Table 2: Average concentration (mean mg/kg, mg/g ± SD, n=3) of metals in Khat leaf samples.
Iron was the most abundant metal in Khat varieties among the tested heavy metals of all the sample sites ranging between (573.33 ± 5.62-741.67 mg/kg) where the highst value is found for koyra Khat and the lowest value for Gidole Khat. The highest level of Fe in Khat plant is consistent with the highest amount of organic matter in all soils of Khat farms. According to ref. [7,9] availability of iron to plant increases with organic matter contents of the soil. Iron reduction was greatly increased with the application of organic matter and the longer the period of submergence, the greater the amount of water soluble plus exchangeable iron.
The amount of zinc in Khat samples varies from 137-203 mg/ kg. The highest being for Koyra Khat and lower in Konso Khat. The concentrations of Mn, Cu and Cr obtained are given as follows: Mn (27.206-85.29 mg/kg), Cu (16.9-22.5 mg/kg) and Cr (1.52- 4.09 mg/kg).
Moreover, the concentrations of toxic metals Pb and Cd in the studied Khat plant leaves were too low to be detected except for Cd present in Konso Khat. This may be due to the fact that there are insufficient factories in the studied areas to cause substantial pollution. As people may have some health problems which may restrict the consumption of electrolytes, knowing the level of these elements in samples consumed is critical. The toxic metals should be thoroughly surveyed in order to take any remedial actions. Control authorities in the national government positions should be aware of such issues and take appropriate measures.
Levels of essential and non-essential metals soil samples
In Table 3 shows the mean concentration values of each metal in each soil sample with its respective relative standard deviation (RSD) in dry-weight basis. It was shown that the macro, micro and toxic metal contents analyzed in the Khat farm soils varied significantly from site to site. Among the macro elements, K content of the soils was higher within a range of (7.26 -12.06) mg/g followed by Mg (0.82-2.477) mg/g and Ca (0.51-1.02) mg/g. Fe from micro elements is the predominant metal with in the concentration range of (12.24-37.2) mg/g. These may be in good agreement with the analysis raised by ref. [8,12] soils with low pH contain high amount of Fe and Al oxides. This is indicative of presence of excess amount of hematite (Fe2O3). Further, Iron makes up about 5 percent by weight of the earth`s crust and must be present in all soils. Common minerals of Fe are Olvine [(Mg,Fe)2SiO4], Pyrite (FeS), Siderite (FeCO3), hematite (Fe2O3), goethite (FeOOH), magnetite (Fe3O4) and ilmenite (FeTiO3) . Iron is absorbed by the plant as Fe2+ and it can be transported to root surfaces as iron chelates.
|Ca(mg/g)||1.02 ± 0.06||0.73 ± 0.03||0.51 ± 0.07|
|Fe(mg/g)||12.24 ± 0.61||17.67 ± 0.71||37.24 ± 0.86|
|Mg(mg/g)||2.477 ± 0.10||0.82 ± 0.07||1.12 ± 0.02|
|Cr(mg/kg)||65.2 ± 5.67||43.68 ± 4.02||18.11 ± 1.02|
|K(mg/g)||12.06 ± 0.62||7.26 ± 0.29||7.29 ± 0.34|
|Cd(mg/kg)||0.6 ± 0.07||0.2 ± 0.04||ND|
|Zn(mg/kg)||176 ± 2.00||123 ± 1.00||141 ± 3.21|
|Mn(mg/g)||0.447 ± 0.045||0.587 ± 0.018||1.25 ± 0.09|
|Cu(mg/kg)||17.5 ± 0.62||49 ± 2.12||31.2 ± 2.31|
|Co(mg/kg)||1.314 ± 0.08||0.895 ± 0.05||0.187 ± 0.01|
Table 3: Average metal concentrations (mean mg/kg, mg/g ± SD, n=3) in the soils of selected Khat growing farms.
The lowest value of K was found in soil derived from the roots zones of Gidole Khat. While the highest concentration of K in the soil of Konso sample site was recorded. The highest amount of Ca contents in the soils derived from the root zones of Konso Khat, is highly consistent with its soil CaCO3% values ranging from 2.94 to 3.8%. As can be seen from Table 3, the levels of concentrations for metals Mn, Zn, Cu and Cr are in the range 0.447-1.25 mg/g, 123-176 mg/kg, 17.49-49 mg/kg and 18.11-65.2 mg/kg respectively. The average concentration of Mn in the earth`s crust is 1000 ppm. There are a number of minerals which contain Mn in sufficient quantity namely, pyrolusite (MnO2), hausmannite (Mn3O4), manganite (MnOOH), rhodochrosite (MnCO3). It is the second highest amount among the micronutrients in the studied soil next to Fe. However, the amount of Mn in Khat leave is present in lower amount relative to its value in soil. According to ref. [3,4,6,7,9]. This is due to the fact that the pH of the studied soil farms ranges from 6.17-8.11. Soils with pH less than 5.5 may contain a large amount of Mn2+in the water soluble and exchangeable form. With increasing soil pH, Mn2+is converted into its higher oxides (Mn3+ and Mn4+) which are insoluble in water and therefore, unavailable to plants.
On the other hand, Cd and Co was detected in most of the analyzed soils of farm lands except in koyra farm. The level of the toxic heavy metal Cd ranges from not detected to 0.6 mg/kg. The level of Pb, the other tested heavy metal, in the soils of all studied farms was found to be below the detection limit of the method used in this study. Comparing the metal concentration in soil with guidelines for soils showed that all the metal concentration is below the guidelines for soils. This implies that the investigated farm soils are more or less free from heavy metal contamination.
The soil pH of the farms ranged from 6.17-8.11. Which categorizes the soils from weakly acidic to weakly alkaline. As shown in Table 4, soils in Koyra had lower pH than those from konso or Gidole. The acidic condition in soil may be due to application of NPK fertilizers. The increased use of nitrogenous fertilizers generally increases soil acidity . Soil pH is one of the most influential parameters that control conversion of immobile solid-phase metal forms to more mobile and/or bio-available solution-phase forms. The solubility of heavy metals is generally greater as pH decreases within the pH range of normal agricultural soils (approximately pH 5.0 to 7.0) [12,15]. Therefore high pH values of soils in this study could have accounted for the low transfer of metals from soil to plants.
|pH(H2O) at 26°C||7.94 ± 0.11||8.11 ± 0.02||6.97 ± 0.07|
|pH(KCl) at 26°C||5.97 ± 0.31||6.97 ± 0.02||5.83 ± 0.02|
|Organic matter (%)||2.16 ± 0.13||2.96 ± 0.11||4.22 ± 0.09|
|CaCO3 (%)||2.94 ± 0.20||3.6 ± 0.08||0.765 ± 0.12|
Table 4: CaCO3, Organic matter content and pH of the Khat growing farms.
Amount of organic matter
Organic carbon of the soil was determined by using Walkely and Black method by dichromate oxidation technique . Soil organic matter is oxidized under standard conditions with potassium dichromate in concentrated sulfuric acid. A measured amount of K2Cr2O7 is used in excess of that needed to destroy the organic matter and the excess is determined by titration with ferrous ammonium sulfate or ferrous sulfate solution, using 1,10-phenanthroline ferrous sulfate indicator to detect the first appearance of unoxidized ferrous ion [16,17]. The organic carbon content of the soil samples were ranged from 2.16% in Konso to 4.22% in Koyra. The higher amount of organic matter content of the Koyra soil is probably due to the farmers’ use of debris of plant tissues locally known as compost which has higher amount of organic carbon [7,15].
Further, the calcium carbonate content of the soil was determined by acid neutralization method. In this method, soil is treated with excess of standard HCl to decompose carbonates. The excess acid is back titrated with 0.1N NaOH after filtration. From the amount of acid required to neutralize the carbonate, the CaCO3 equivalent is calculated. The amount of CaCO3 determined is generally higher because some of the acid is used up by exchangeable Ca and Mg, sodium carbonate if present and possibly also by reaction with primary minerals .
The result from Table 4 reveals, calcium carbonate content of the soil was high in Gidole which is 3.6% and low in Koyra which is 0.765%. This is consistent with the results of the soil acidity in which the moderately alkaline soil of Gidole contains large amount of CaCO3 content, while the acidic soil of Koyra has negligible amount of values of CaCO3 content.
Comparison of the concentration of identified metals in soil and Khat leaves
To check associations of the same metal in soil with leaves as well to check whether the ions of one kind present in the soil, either facilitate or interfere with the uptake of the other kind of ions, Pearson Correlation coefficient was used. Thus, Pearson product moment correlation coefficient was calculated. Based on the results of r- value, the studied soils were found that correlation coefficients in Koyra and Gidole Khat were lower and, in some cases, not significant, either at 0.05 or at 0.01 level. Some heavy metal concentrations (Fe, Cr, Zn and Mn) in Khat were negatively correlated with soil pH. Soil pH is the dominant factor controlling Khat metal uptake. This may be because bioavailability of metals increased with decreasing soil pH. Agronomic practices that reduce soil acidification may be helpful in minimizing the bioavailability of heavy metals in soil, thus resulting in reduced plant uptake and accumulation in plant clones.
The increasing value of Ca contents in Khat leaves are in accordance with increasing values of CaCO3% contents in soil. Interactions among the coexisting elements occurring in the root surface and within the plants also affect their uptake and accumulation in plants. Metals interact with other metals in the uptake process by the plants. This is because of some metals have similar chemical properties. For example, Mn shows properties of both the alkaline earth cations such as Mg2+and Ca2+and the heavy metals (Zn, Fe). Therefore, it is noted that these ion species affect the uptake and translocation of Mn in the plants. According to ref. [17,18] with increasing application of potassium the content of Fe and Cu decreases in plant tissues. Therefore, the interaction of K with Fe and Cu is antagonistic. K is negatively interacted with both Cu and Fe in Khat leaves. Zn in this study interacts negatively with that of Cu and Mn content in the Khat leaves. The above reports showed that increasing the Zn content decreases the toxic effect of Mn and Cu, thus implying a mutual antagonistic effect.
Generally, positive correlations were observed between total metals in soil and Khat leaves metal content for all the elements studied. Correlation coefficients were ranging 0.244-0.951. However, no strong correlations between the leaves and other variables are evident except for Ca and Mg metal contents for soil and Khat leaves. This can lead to the probable conclusion that the concentration of metals in the soil measured by the total metal determination method cannot be used as a predictor of metal concentration in Khat leaves [19-23]. This may be due to the fact that total metal contents of a soil contains all exchangeable metals, carbonate bound metals, oxides bound metals, organic matter bound metals and residues, while metals which are bioavailable for plant uptakes are found in the exchangeable metals. Additionally, Regression analysis was conducted between total metals in soil and Khat leave metal contents to examine their correlation. The total metal values in soil and Khat leaf metal contents fit a linear regression model for all metals studied. However, there was a certain difference in the predicting accuracy among the metals, as R2 ranged from 0.281 for Mn to 0.991 for Mg. almost all the regression values lie around 0.4 which shows that their correlation is not as much stronger.
Khat is clearly an important cash crop in Ethiopia well-known for its foreign currency earnings. Nonetheless, it is little understood and given no development and research attention as yet. This study has helped underline the considerable level of sophistication that characterizes the cultivation and marketing of Khat in eastern Ethiopia. In this study the levels of metal in edible portion of Khat leaves and their respective soil samples collected from southern region of Ethiopia were analyzed for their contents of Ca, Fe, Mn, Cu, K, Zn, Co, Cr, Mg, Pb and Cd using flame atomic absorption spectrometer. The optimized wet digestion method for the analysis of samples was found efficient for all the metals and it was evaluated through the recovery experiment and a good percentage recovery of 90 to 110% was obtained for all the metals identified.
Based on the results of this study, higher amounts of K and Fe among the determined macro- and micronutrient metals respectively for both Khat leaves and soils. Heavy metals Cr and Cu were found to be comparatively at lower levels in most of the soil and Khat samples. Concentrations of K, Ca and Mg in the edible leaves of Khat plants decreased in the order: K>Mg>Ca. While for the soils the order is; Fe>K>Mg. Heavy metal contents in all Khat samples could be arranged in descending order: Fe>Zn>Mn>Cu>Cr. While for soil samples the order is Fe>Mn>Zn>Cu>Cr. The levels of Cd and Pb in all Khat samples were too low to be detected by the method used in this study. However, their absence in the studied Khat plants of the farms may not necessarily guarantee the nonexistence of these toxic metals in the different commercial Khat plants available in markets. Further, the total metal values in soil and Khat leaf essential and non-essential metal contents fit a linear regression model for all metals studied. However, there was a certain difference in the predicting accuracy among the metals, as R2 ranged from 0.281 for Mn to 0.991 for Mg.
Finally, the overall results of this study suggest that there were significant variations in the level of some elements between the Khat varieties which could be attributed to different factors such as age of the harvest Khat, geographical and climatical variation, difference in physicochemical nature of the soil, and different agricultural practices among Khat cultivars.
The authors are grateful to the Department of Chemistry, College of Natural Sciences, Arbaminch University, Arbaminch, Ethiopia for proving laboratory facilities. We are thankful to our students (graduating class of 2014) for helping in sample collection and laboratory work.