Rhodium Citrate Associated with Maghemite Nanoparticles Causes DNA Fragmentation Independently of Caspases 3 and Mediated by Reactive Oxygen Species

Breast cancer is the most common cancer among women, excluding non-melanoma skin cancer. Research efforts have been directed towards the development of more efficient drugs against this disease, such as metal complexes, which have been widely studied. These compounds can intercalate in DNA bases and impair DNA transcription and replication, leading to cell death. Cell death can also be associated with early induction of reactive oxygen species (ROS) production by cells treated with this kind of metal complex. Nevertheless, the use of these compounds is limited because of their systemic toxicity. In this regard, the use was proposed of dirhodium citrate [Rh2(H2cit)4] associated with magnetic nanoparticles (NPs), which are carriers that may work in decreasing systemic toxicity. We compared cell viability effects of free Rh2(H2cit)4, Rh2(H2cit)4-loaded maghemite NPs [Magh-Rh2(H2cit)4] and maghemite nanoparticles loaded with citrate (Magh-cit), on MCF-7 breast cancer cells and MCF-10A non-tumor and non-tumorigenic epithelial cells by MTT assay. Furthermore, we examined whether the NPs induce cell death by apoptosis in a cell line without caspase 3 expression (MCF-7). This feature was demonstrated by quantification of ROS through labeling cells with DCFDA, DNA fragmentation studies analyzed with a flow cytometer, release of cytochrome C from mitochondria assays, and effector caspases activation analysis (revealed by FLICA) as visualized by confocal microscopy. Our results confirmed that rhodium citrate was less cytotoxic in its free form than when associated with the tested drug delivery system. Moreover, Magh-Rh2(H2cit)4 NPs and Magh-cit NPs induced apoptosis cell death mediated by ROS and independently of caspase 3 expression. *Corresponding author: Sônia Nair Báo, Institute of Biological Sciences, Department of Cell Biology, University of Brasília (UnB), Brazil, Tel: +556131073300; E-mail: snbao@unb.br Received June 06, 2015; Accepted July 20, 2015; Published July 30, 2015 Citation: Chaves NL, Lopes CAP, Carneiro MLB, Souza ARD, Silva MOD, et al. (2015) Rhodium Citrate Associated with Maghemite Nanoparticles Causes DNA Fragmentation Independently of Caspases 3 and Mediated by Reactive Oxygen Species. J Nanomed Nanotechnol 6: 312. doi:10.4172/2157-7439.1000312 Copyright: © 2015 Chaves NL, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Introduction
Since metal compounds exhibit antiarthritic, antibacterial, anti-hypertensive, antidepressant and anticancer properties, these therapeutic agents have come into routine clinical use. Similarly to cisplatine, a metal compound with anticancer activity, rhodium (II) citrate has emerged as a potential antitumor agent [1]. Nevertheless, severe side effects of these treatments [2] remain a limitation and have stimulated research towards the development of delivery systems in order to improve specificity.
Nanocarriers have the potential to improve the therapeutic index of currently available drugs by increasing drug efficacy, lowering drug toxicity, and achieving steady state therapeutic levels of drugs over an extended period of time [3,4]. The association of rhodium (II) citrate with magnetic nanoparticles has the potential to increase the specificity of the cytotoxic action, since these complexes can be guided or transported to a target by the use of an external magnetic field [5,6]. This approach can also allow its use to produce magnetic hyperthermia [7][8][9]. This more specific action may have special applications in breast cancer therapy, because adverse effects of the current treatments and remission risk are prominent issues. Furthermore, the high mortality rates and severity of this problem make novel therapy investigations urgent [10][11][12].
Metals or reactive oxygen species (ROS) generated by metals in the cells can directly initiate the intrinsic apoptotic pathway at the mitochondria or indirectly trigger this pathway by p53 activation as a result of DNA damage induced by metal or ROS [13,14]. The p53 activation leads to the expression of Bax and Noxa, two proapoptotic proteins which induce an increase in mitochondrial permeability [15]. In this circumstance, cytochrome C is released into the cytosol from damaged mitochondria and binds to Apaf1, resulting in caspases activation [16,17].
Apoptosis regulation defects are the main factors that contribute to carcinogenesis and cancer progression. These problems also figure prominently in the resistance to chemotherapy, radiotherapy, hormonal therapy and immune-based treatments [18,19]. Currently, it is recognized that many efficient chemotherapeutics act by triggering any of the apoptosis pathways [20]. Depending on the involvement of different caspases activation mechanisms, cell death by apoptosis can be roughly divided into two categories: (1) receptor mediated 'extrinsic' apoptosis (death ligands with caspase 8 activation) and (2) mitochondria-mediated 'intrinsic' apoptosis (cytochrome C release and caspase 9 activation) [21]. Both types of apoptosis induction pathway converge to the activation of the same effector caspases, mainly caspase 3, in order to complete the apoptotic process [16,22].

Nanomedicine & Nanotechnology
Caspase 3 has been considered essential for certain processes associated with the dismantling of the cell and the formation of apoptotic bodies. This caspase is activated after barrier mitochondrial membrane rupture [23]. By the proteolysis of substrates, caspase 3 mediates some of the typical morphological and biochemical changes associated with apoptosis, such as the cleavage of a-fodrin, gelsolin, kinase-associated rho-1 (ROCK-1) and p21-activated kinase 2 (PAK2), which contribute to membrane blebbing [24], cleavage of the inhibitor of caspase-activated DNAse (ICAD) and lead to the pattern of DNA fragmentation typically observed in apoptosis [25,26].
The MCF-7 cells do not express caspase 3. Despite this, MCF-7 cells can exhibit morphological changes consistent with apoptosis, including DNA fragmentation, following treatment with a variety of agents and conditions [27]. Thus, it was suggested that caspase 3 may not be essential for DNA fragmentation during apoptosis in some instances [28,29]. It has already been suggested that rhodium citrate could induce apoptosis in MCF-7 cells [30]. However, this induction was not confirmed when the association of rhodium citrate with maghemite NPs was employed, and the mechanism of this process is not clear.
In the present study, we investigated cell death by apoptosis induced by rhodium (II) citrate loaded-maghemite nanoparticles in MCF-7 breast cancer cells, with special consideration for the dependence of caspase 3 and ROS as mediators.

Cell culture
The human breast cancer cell line MCF-7 was obtained from the American Type Culture Collection (ATCC) and cultured in Dulbecco's modified Eagle's medium containing 1% (v/v) penicillin-streptomycin . The cells were maintained in a 5% CO 2 humidified incubator at 37°C. At 85% confluence, cells were harvested using 0.25% trypsin (Gibco, Life Technologies, Carlsbad, CA, USA) and were subcultured in 75 cm 2 flask or 6, 12 or 96-well plate according to the experimental procedures. The cells were allowed to attach to the surface for 24 hours prior to the treatments. The Rh 2 (H 2 cit) 4, Magh-Rh 2 (H 2 cit) 4 and Maghcit nanoparticles were suspended in cell culture medium, diluted to the defined concentrations and then added to the attached cells for the time required in each experiment.

Nanoparticles characterization
The diameter distribution and surface charge of the nanoparticles were analyzed by using the Zetasizer Nano ZS machine (Malvern, Malvern,UK).
The assay was performed at 25°C with nanoparticles from the stock solutions [Magh-Rh 2 (H 2 cit) 4 with 2×10 16 iron particles/ml and Maghcit with 2.13×10 16 iron particles per ml at the dilution of 1:1000 in ultrapure water.

Transmission electron microscopy
Morphology and size of the nanoparticles were evaluated by transmission electron microscopy (TEM). The stock solutions of Magh-Rh 2 (H 2 cit) 4 and Magh-cit were diluted 1:100 and deposited on 300-mesh grids coated with formvar-carbon film and dried at room temperature. Subsequently, nanoparticles were observed under a JEM-1011 transmission electron microscope (JEOL, Tokyo, Japan) operating at 100 kV, and the images were acquired by an Ultrascan camera (Gatan, Pleasanton). Nanoparticles (n=120) were measured by using the Image Pro-Plus 5.1 software, and data were adjusted by log normal distribution in a Gaussian curve in order to obtain the modal diameter.

MTT assay
In order to understand how cells respond to Magh-Rh 2 (H 2 cit) 4 nanoparticles, MCF-7 cells were incubated for 24 h with various concentrations of this compound. Furthermore, the tumor cells were also exposed to free rhodium citrate and maghemite nanoparticles at the same concentrations as those in Magh-Rh 2 (H 2 cit) 4 . The MCF-10A non-tumor cells were used to assess effects of the treatments on normal cells.
Viability of MCF-7 and MCF10-A cells after exposure to NPs and Rh 2 (H 2 cit) 4 was evaluated by the MTT assay according to the manufacturer's recommendations (Invitrogen, Life Technologies, Carlsbad, CA, USA). Briefly, 1×10 4 cells/well were seeded in 96-well plates and exposed to the treatments at the concentrations of 50 μM, 200 μM, 300 μM (rhodium citrate) or 3 mM, 12 mM and 19 mM (iron) for 24 h. All experiments were repeated at least three times in triplicates. Results were expressed as percent of viability (%V) according to the following formula (blank discounted): %V = absorbance (cells + medium + NPs) -absorbance (medium + NPs). After cell treatment, the medium was removed from each well and replaced with new medium containing MTT (15 μL of MTT solution at 5 mg/ml and 135 μL of culture medium) for two and a half hours at 37°C in humidified atmosphere with 5% CO 2 . The resulting formazan product was dissolved in 200 μL of dimethyl sulfoxide (DMSO, Sigma-Aldrich, St Louis, MO, USA). Afterwards, the supernatants of each sample were transferred to a fresh 96-well plate (to avoid the interference of nanoparticles that did not enter the cells and accumulate at the bottom of the wells) and absorbance was measured by a spectrophotometer (SpectraMax M2, Molecular Devices, Sunnyvale, CA, USA) at the 595 nm wavelength.

Laser scanning confocal microscopy analyses
Cytochrome C location, DNA fragmentation assessment by TUNEL and caspases 6 and 7 detection were performed by using laser scanning confocal microscopy. MCF-7 cells (2×10 5 ) were seeded onto glass coverslips placed in the bottom of 24-well plates and treated with Magh-Rh 2 (H 2 cit) 4 (300 μM) and Magh-cit (19 μM Iron). Afterwards, for DNA fragmentation and cytochrome C location analyses, cells were washed twice with PBS, fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in 0.1% sodium citrate. Thereafter, for DNA fragmentation evaluation, cells were incubated with fluorescein-tagged dUTP in the presence of terminal deoxynucleotidyl transferase (Roche Applied Science, Indianapolis, IN, USA) for one hour in the dark. In the other examination, MCF-7 cells were blocked by incubation for 2 h in PBS buffer containing 3% fetal bovine serum, incubated with anti-cytochrome C (1:200; Invitrogen, Life Technologies, Carlsbad, CA, USA) for two hours, washed with PBS and incubated with a secondary antibody conjugate (Alexa-fluor 488 from Invitrogen, Life Technologies, Carlsbad, CA, USA) and red mitotracker (Invitrogen, Life Technologies, Carlsbad, CA, USA) for cytochrome C location analysis. The cells were also incubated with DAPI (0.5 g/ml) for nuclei staining. For the caspases 3/7 and 6 activity assay, the apoptosis detection kits from Immunochemistry Technologies (Bloomington, MN, USA) were used according to the manufacturer's instructions. These caspase detection probes are comprised of an affinity peptide inhibitor sequence, a fluoromethyl ketone (FMK) moiety that facilitates an irreversible binding event with the activated caspase enzyme, and a fluorescent tag reporter. The cells were incubated with the Fluorescent Labeled Inhibitor of Caspases 3/7 (FLICA-SR) and caspase 6 (FLICA-FAM) and maintained for 1 h at 37°C in a CO 2 incubator. Subsequently, the Hoechst stain was used to label the nuclei (200 μg/ml). The cells were washed twice (wash buffer) and fixed with a formaldehyde fixative solution (1:10) in wash solution. Coverslips were then mounted onto microscope slides using an anti-fade mounting medium, and incubated at 4°C in the dark. All assays described above were analyzed on a laser scanning confocal microscope Leica TCS-SP5 (Leica, Mannheim, DE).

Measurement of reactive oxygen species (ROS)
Reactive oxygen species generation was measured by staining MCF-7 cells with 5-(and-6) carboxy-2′,7′-dichlorodihydrofluorescein diacetate (DCFDA, Sigma-Aldrich, St Louis, MO, USA). Cells were exposed to Magh-Rh 2 (H 2 cit) 4 (300 μM) or Magh-cit (19 μM iron) for 1.5 h, then medium was discarded and cells were washed with PBS and incubated with 20 μM DCFDA in culture medium without phenol red under low-light conditions. The cells were incubated for 30 min at 37°C and the fluorescence emission (excitation at 488 nm and fluorescence emission at 535 nm) from DCFDA was analyzed by using a plate reader (SpectraMax M2, Molecular Device) and laser scanning confocal microscope Leica TCS-SP5 (Leica). Hydrogen peroxide (100 mM for 20 min) was used as positive control in order to validate the protocol.

Statistical analysis
Values are expressed as the mean ± standard error of the mean. Each value is the mean of at least three independent experiments with each group. Comparisons of treatments against controls, the effects of exposure time and concentration of maghemite nanoparticles were made by using two-way analysis of variance (ANOVA) followed by Bonferroni's post hoc test. One-way analysis of variance (ANOVA) followed by Tukey's multiple post hoc comparison were used to evaluate intracellular reactive oxygen species production. All analyses were performed with the GraphPad Prism (v. 4) software. The asterisks (*) indicate that values are significant (*p<0.05, **p<0.01 and ***p<0.001).

Cytotoxicity induced by Magh-Rh 2 (H 2 cit) 4 , Magh-cit nanoparticles and Rh 2 (H 2 cit) 4
A decrease was observed in cell viability induced by the drug and nanoparticles in a dose-dependent manner (

Estimation of intracellular ROS induced by Magh-Rh 2 (H 2 cit) 4 and Magh-cit nanoparticles
In order to evaluate the oxidative stress induced by maghemite nanoparticles, the ROS production was quantified in treated tumor cell samples. After one and a half hours, a significant increase was observed in ROS production in MCF-7 cells induced by Magh-Rh 2 (H 2 cit) 4 treatment, as compared to the control samples and samples treated with Magh-cit. The ROS quantification was estimated by DCFDA fluorescence intensity (Figure 4).

Magh-Rh 2 (H 2 cit) 4 and Magh-cit nanoparticles alter mitochondrial membrane potential causing cytochrome C release
This analysis aimed to determine cytochrome c activation and translocation in MCF-7 cells. In cell samples treated with 10 nM paclitaxel for two hours ( Figures 5A-C), the cytochrome C labeling (green) predominated over the mitochondrial tag (red). This feature is better shown in the overlay images ( Figure 5C). This pattern suggests that the proteins are no longer confined within mitochondria. In untreated cell samples ( Figures 5D-F), cytochrome C labeling coincides with mitochondria labeling, showing a merged fluorescent yellow in the overlay images ( Figure 5F). The yellow color means that cytochrome C localization is the same as that of the mitochondria. In the groups of cells treated with 300 μM Rh 2 (H 2 cit) 4 -loaded maghemite NPs [Magh-Rh 2 (H 2 cit) 4 ] (Figures 5G-I) or 0.019 mol/L (iron) maghemite nanoparticles loaded with citrate (Magh-cit) for 24 hours, the results were similar to those already described for the positive control comprising cells treated with paclitaxel. Therefore, it is suggested that both of these test treatments were able to induce the release of cytochrome C into the cytoplasm in MCF-7 cells (Figures 5J-L).

Magh-Rh 2 (H 2 cit) 4 and Magh-cit induced caspases 6 and 7 activation in MCF-7 cells
Effector caspases 6 and 7 were present in active forms after 12 hours of treatment with Magh-Rh 2 (H 2 cit) 4 and Magh-cit ( Figures 6A-I). The fluorescent labeling of caspases was accompanied by the occurrence of pyknotic nucleus (revealed by DAPI labeling), which is another key feature observed in apoptotic cells (Figures 6A-I). The control cells (untreated) showed no labeling of activated caspases. When both caspases were labeled simultaneously in the cells treated with the two compounds, it was observed that the two caspases are activated at the same time.

Magh-Rh 2 (H 2 cit) 4 and Magh-cit induced apoptosis with DNA fragmentation in MCF-7 cells
In order to analyze whether Magh-Rh 2 (H 2 cit) 4 and Magh-cit nanoparticles promote cell death with DNA fragmentation in MCF-7 cells, despite the absence of activated caspase 3, flow cytometry and laser scanning confocal microscopy of TUNEL-labeled cells were performed. In the flow cytometry analysis, it was observed that the nanoparticles induced DNA fragmentation in MCF-7 cells ( Figures 7A and B), which was significantly higher than that in the control cells after 36 hours of the stimulus with 300 µM Magh-Rh 2 (H 2 cit) 4 or 19 mM (iron) Maghcit. However, no statistical difference was observed between these two treatments, which displayed TUNEL labeling profiles similar to that of the positive control ( Figure 7C).

Discussion
In this work, we confirmed that the association of maghemite nanoparticles and rhodium citrate enhances their effects on breast cancer cells in a more specific way. This enhancement process observed with drug association to nanostructures is widely described by other authors [31][32][33]. Moreover, even in the absence of the associated drug, maghemite nanoparticles containing only citrate can promote damage to tumor cells, once again in a specific manner as compared   to normal breast epithelial cells. However, these nanoparticles (Maghcit) form precipitates in culture medium (DMEM) earlier than Magh-Rh 2 (H 2 cit) 4 . The formation of aggregates in media is an obstacle to the employment of magnetic nanoparticles for in vitro and in vivo tests [34,35]. Thus, Magh-cit was less stable than Magh-Rh 2 (H 2 cit) 4 for our analyses.
It has been suggested that the physicochemical properties of Rh 2 (H 2 cit) 4 -loaded maghemite NPs should be promising for use in cancer treatment [30]. The metal complexes analyzed, in the present work, were shown to be anionic. This characteristic can be related to a high affinity for cellular membrane in a few cationic sites, similarly to what may occur in the adsorption of anionic ferritin [36][37][38][39].
The monodispersity feature observed in the nanoparticles of this work was also found in other studies on the characterization of maghemite nanoparticles without using any size selection tool [40,41]. This feature is a key property for the production of nanoparticles for biomedical applications. Polydispersity indexes like those recorded in this analysis (monodisperse), reflect the narrow distribution of nanoparticle sizes and homogeneity, indicating a high level of complex diameter control.
In the cytotoxicity evaluation, we observed that free Rh 2 (H 2 cit) 4 at concentrations of up to 300 µM did not significantly reduce breast cancer cells' viability. Carneiro and co-workers [30] obtained similar results for the same complex and concentrations after 24 and 48 hours' treatments in MCF-7 cells (viability of 85% and 73%, respectively). However, when combining Rh 2 (H 2 cit) 4 with maghemite nanoparticles, a higher reduction in tumor cell viability was observed than with the free complex. The number of non-viable cells was higher in tumor cells (MCF-7) than in normal cells (MCF-10A), particularly at the concentrations of 200 µM and 300 µM (after 24 hours). Similarly, in our work, which compared 50 µM rhodium citrate free or associated with nanoparticles (3×10 15 iron particles/ml), the association with maghemite nanoparticles enhanced the cytotoxic action of rhodium citrate. Moreover, for the same iron concentration, there have not been reports of viability reduction promoted by maghemite nanoparticles functionalized with citrate only (Magh-cit) for the strains tested for 24, 48 and 72 hours [30]. In the present study, a cytotoxic action of this nanoparticle was demonstrated, which was higher in MCF-7 cells than in the untreated cells after 24 hours of treatment at a concentration of 19 mM iron (2.4×10 16 particles of iron/ml). However, this cytotoxicity was not statistically different from the values found for Magh-Rh 2 (H 2 cit) 4 and, as already mentioned, (Magh-cit) was less stable in culture medium, hindering its application. The cytotoxicity promoted by Magh-cit in MCF-7, in this study, could be attributed to the greater number of iron particles/ml (2.4×10 16 ) found in our compound compared to the nanoparticles used in the study by Carneiro and colleagues [30].
Even being biocompatible, compounds containing iron can be associated with cellular cytotoxicity mediated mainly by redox mechanisms that cause oxidative stress [42,43]. The tests for ROS production by tumor cells treated with our metal compounds showed that oxidative stress may be the initial signal that induces cell death after one and a half hours of treatment. On the other hand, there was not   any increased production of ROS in Magh-cit treated cells as compared to the control sample and the other groups, indicating that these two nanoparticles can induce cell death by different mechanisms, or that Magh-cit is not being internalized by the cell in the same manner as Magh-Rh 2 (H 2 cit) 4 . The size of the nanoparticles or clusters formed by them is strongly associated with cellular uptake and death induction mechanisms [44][45][46]. Studies of colloidal stability of these kinds of nanoparticles showed changes in stability and a tendency for the formation of clusters associated with pH and ion concentration in the culture medium [47]. Although a general description is still missing, it is believed that the colloidal destabilization of Magh-cit in biological fluids results from the adsorption of biological molecules, but also from the exchange and/or the removal of the protecting layer. As a result, the original coating is not sufficient to offset the van der Waals attraction, which leads to aggregation, as demonstrated in the study by Safi and colleagues [48].
Another candidate for the cellular damage caused by the nanoparticles is the loading component, citric acid. Citrate is formed by condensation of acetyl-coenzyme A and oxaloacetate and plays a key role in the regulation of glycolysis. Thus, it may be related to cytotoxic activity in tumor cells that exhibit an increased anaerobic glycolysis [49,50]. In accordance with the findings of Zhang and co-workers [51], who reported that citrate may exert a cytotoxic effect on mesothelioma MSTO-211H cultured cells, this molecule can be important not only to functionalize nanoparticles, but also to exert a synergistic effect on the cytotoxicity in tumor cells.
Finally, we also found that rhodium citrate associated with maghemite nanoparticles induces death in breast cancer cells with active caspases and DNA fragmentation. From our cell model, it was easy to determine that such cell death occurs independently of caspase 3 activation. Nevertheless, other effector caspases may be involved in this process [52]. The compounds Magh-Rh 2 (H 2 cit) 4 and Magh-cit at the concentrations of 300 µM (rhodium citrate) and 19 mM (iron), respectively, induced cytochrome C release after 24 hours. This event is characteristic of apoptotic cell death via the mitochondrial pathway. The activation and dispersion of cytochrome C is an initial stage of the apoptosis process, which results from the alteration of the mitochondrial membrane potential [53]. Although the type of tumor cell tested (MCF-7) does not express caspase 3, which characterizes it as a cell resistant to apoptosis [54], apoptosis in MCF-7 cells has already been described, induced by several compounds, including other DNA damaging agents. Xue and colleagues [55] observed that staurosporine induces apoptosis in this cell type, but not all mitochondrial changes inherent to this process were always present [56].
It was shown that cytochrome C release could be independent of caspases activation [57]. It was further noted that caspase 8 can also cleave Bid (BH3 domain-containing proapoptotic members of the Bcl-2 family) to their truncated form (tBid). Translocation of tBid to mitochondria induces mitochondrial release of cytochrome C and subsequent caspase 9 activation, thus amplifying the death signal [58]. Our model excludes any relationship between the mechanism involved in cytochrome C release from mitochondria and caspase 3 activation induced by Magh-Rh 2 (H 2 cit) 4 and Magh-cit.
In addition to the cytochrome C release, caspases (6 and 7) activation, another apoptosis marker, was found following treatment for 12 hours with 300 µM Magh-Rh 2 (H 2 cit) 4 and 19 mM (iron) Magh-cit. The caspases, proteases of the cysteine family, are the main components of the execution phase of the programmed cell death. The initiator caspases (2, 8, 9 and 10) are first induced to oligomerization, leading to autocatalytic activity and subsequent cleavage of effector caspases [53]. In the pathway initiated by caspase 9, cytoplasmic cytochrome C triggers apoptosome formation, which is a multi-protein complex containing cytochrome C, dATP, Apaf-1 (Apoptotic protease-activating factor 1) and pro-caspase 9 [58,59]. It has already been reported that besides cytochrome C release from mitochondria, effector caspase activation (caspase 7) was also observed after ultraviolet radiation treatment of MCF-7 cells [60]. Since MCF-7 cells were induced morphologically and physiologically to cell death by apoptosis, it is suggested that there is an alternative pathway to apoptosis initiation. It has been proposed that initiator caspases with long pro-domains, such as caspases 8 or 9, can lead to the induction of apoptosis by activating effector caspases other than caspase 3, such as caspase 7 [61,62]. Along with caspase 7, another important component of the apoptosis cascade is caspase 6, which was also identified in its active form in this work. Caspase 6 is responsible for cleaving nuclear lamins, thus triggering cell death [63]. Inhibition of caspase 6 protects MCF-7 cells from apoptosis induced by the anti-mitotic agent neocarzinostati [64]. Therefore, identification of activated effector caspases 6 and 7 in our studies allows us to consider them important candidate mediators of apoptotic changes resulting from treatment with Magh-Rh 2 (H 2 cit) 4 and Magh-cit. Nevertheless, this hypothesis requires confirmatory testing, such as analysis of these events after inhibition of these caspases.
Nuclear DNA degradation in nucleosomes is a characteristic of cell death by apoptosis. This process occurs in response to various apoptotic stimuli in a wide variety of cell types [65]. Molecular characterization of this process has identified a specific DNAse (CAD, caspase-activated DNase), which cleaves the chromosomal DNA in a caspase-dependent manner. CAD is synthesized with the help of ICAD (inhibitor of CAD), which functions as a specific chaperone for CAD. This DNAse is found complexed with ICAD in cell proliferation. When cells are induced to apoptosis, caspases, especially caspase 3, cleaves ICAD, which leads to the dissociation of the CAD-ICAD complex and the release of CAD to cleave the chromosomal DNA [26,66]. Ultraviolet radiation, staurosporine, tumor necrosis factor (TNF) and several other DNA damaging agents are listed as inducers of apoptosis in MCF-7 cells [67,68]. However, in these studies, death occurred in the absence of DNA fragmentation. According to Jänicke [54], caspase 3 activation is essential to the occurrence of this particular event. Conversely, in our studies, maghemite nanoparticles loaded with rhodium citrate or only citrate were able to induce DNA fragmentation in MCF-7 cells as observed in DNA analysis by flow cytometry, which was complemented by morphological analyses provided by the TUNEL assay. It was also demonstrated that DNA fragmentation occurred by the TUNEL labeling method in this cellular type, after treatment with Pbox-6, a member of pyrrolo-1,5-benzoxazepines [27]. These investigators demonstrated that DNA fragmentation was suppressed by caspase 7 inhibition, suggesting that, in the absence of caspase 3, another effector caspase may be the primary mediator of DNA fragmentation. This may also be the explanation for the mechanism of DNA fragmentation induced by Magh-Rh 2 (H 2 cit) 4 and Magh-cit in MCF-7 cells. Liang and colleagues [64] also observed DNA fragmentation in MCF-7 cells after neocarzinostatin treatment. Using a number of caspase inhibitors with overlapping specificities, specific chromogenic enzyme substrates and an antibody specific for activated caspase 7, this group concluded that, for the treatment tested in this strain, the occurrence of apoptosis is dependent on caspases 9, 7 and 6.
Besides the hypothesis of other effector caspases as mediators of DNA fragmentation, some studies support the induction of apoptosis by a caspase-independent pathway [69,70]. In tests with resveratrol (RES), a chemopreventive molecule, DNA fragmentation was observed in MCF-7 cells, even with the inhibition of caspases, thus suggesting a caspase-independent mechanism of apoptosis. This process is mediated by Bcl-2, which promotes a loss of mitochondrial membrane potential and increased production of reactive oxygen species (ROS) [70]. This pathway is a plausible hypothesis to explain the mechanism of death caused by Magh-Rh 2 (H 2 cit) 4 in MCF-7 cells, since these latter events were also evidenced in the present study.

Conclusion
The pattern of cell death features in the absence of caspases demonstrated in our work suggests an atypical activation of apoptosis in MCF-7. Therefore, the mechanisms of DNA fragmentation, as well as apoptosis signaling cascade events induced by Magh-Rh 2 (H 2 cit) 4 and Magh-cit must be further investigated in order to elucidate their cytotoxic action. The data generated by this work may contribute to the development of these nanoparticles as increasingly effective compounds for the treatment of breast cancer. Further studies are being conducted in our lab in order to better understand the mechanism involved in cell death induced by Magh-Rh 2 (H 2 cit) 4 and Magh-cit.