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Scorpine-Like Peptides

Karen Luna-Ramírez1, Juana Maria Jiménez-Vargas2 and Lourival D Possani2*

1Department of Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology, Winchester Str. 2, 35394 Giessen, Germany

2Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca 62210, México

Corresponding Author:
Lourival D Possani
Departamento de Medicina Molecular y Bioprocesos
Instituto de Biotecnología, Universidad Nacional Autónoma
de México, Avenida Universidad, 2001, Colonia Chamilpa Apartado
Postal 510-3, Cuernavaca 62210, México
Tel: +52-77-73121709
Fax: +52-77-73172388
E-mail: [email protected]

Received date: April 05, 2016; Accepted date: May 07, 2016; Published date: May 09, 2016

Citation: Luna-Ramírez K, Jiménez-Vargas JM, Possani LD (2016) Scorpine-Like Peptides. Single Cell Biol 5:138. doi:10.4172/2168-9431.1000138

Copyright: © 2016 Luna-Ramírez K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Scorpine-like peptides are intriguing and unique compounds of scorpion venom. They possess two well-defined regions that confers them bi-functionality. The N-terminal region is similar to scorpion antimicrobial peptides lacking disulfide bridges, whereas the C-terminal region contains six cysteines forming three disulfide bridges that tightly bind the peptide. Scorpine-like peptides have shown activity against bacteria (i.e. B. subtilis, K. pneumoniae, P. aeruginosa), fungi and also as potassium channel blockers. Additionally, they have been successful in controlling malaria and some types of viruses.

Keywords

Scorpion venom; Peptides; Amino acids; Antimalarial activity

Introduction

Scorpion venoms are complex mixture of peptides with a variety of pharmacological functions, specially targeting membrane proteins and interfering with the membrane permeability for Na+, K+, Ca+2 and Cl- in excitable and non-excitable cells [1,2]. Scorpion venoms also contain enzymatic and cytolytic compounds. In general, scorpion venom compounds can be classified in two groups: disulfide-bridged peptides (DBP) that represent neurotoxins [3] and non-disulfide-bridged peptides (NDBP) [4]. Interestingly, scorpine, a compound isolated from Pandinus imperator venom [5], contains a region belonging to the DBP and another belonging to the NDBP. Scorpine was shown to display antibacterial and anti-parasitic activities, but also to modify normal function of potassium ion channels. Scorpine-like peptides have been discovered in other species of scorpions, for example in Hadrurus gertschi [6,7], Tityus costatus [8], Opisthacanthus cayaporum [9], Pandinus cavimanus [10], Euscorpiops validus [11], Urodacus yaschenkoi [12], Opistophthalmus carinatus [13], Heterometrus laoticus [14] and Vaejovis species [15].

Structural Properties and Bioactivity of Scorpine-Like Peptides

The scorpine-like peptides belong to the third group of β-K+ channel specific toxins (β-KTxs) and were first called ‘orphan peptides’ because they showed contrasting pharmacological activity due to their bi-functionality [6,13,16]. The N-terminal region of scorpine-like peptides have cytolytic or antimicrobial activity like the insect cecropins (20% identity) with an alpha-helical structure that moves freely [16,17], while the C-terminal region has K+ channel blocking activity and it is tightly folded by three disulfide bridges exhibiting a “cysteine stabilized α/β motif” (CS-α/β) [18]. The C-terminal domain is characterized by a conserved sequence: (x)3CxA(x)5GxCxHC(x)3ExKxGxCHGTKCKC GxPLSY(x)1-4; obviously containing 3 disulfide bridges and following completely the typical Cys pattern of invertebrate defensins discussed by Froy and Gurevitz [17] (Figure 1). This segment is responsable for the activity on potassium channels (Table 1).The biological activity of some of these peptides have been investigated (Table 1).

Scorpion species Name Length (aa) Biological effects Reference**
Pandinus imperator Panscorpine 75 Active against bacteria (B. subtilis and K. pneumoniae) P56972, [5]
Heterometruslaoticus Heteroscorpine-1 76 Active against B.subtilis, K.pneumoniae and P.aeruginosa. P0C2F4, [14]
Opistophthalmuscarinatus Opiscorpine-1 76 The short synthetic peptide (20-54aa) has antifungal activity against F. culmorum(IC50= 8.8 µM), F. oxysporum(IC50= 10 µM) and bacteria P. aeruginosa, E. coli. Q5WR03, [13]
Opiscorpine-2 76 Active against fungi and bacteria* Q5WR01
Opiscorpine-3 76 Active against fungi and bacteria* Q5WQZ7
Opiscorpine-4 76 Active against fungi and bacteria* Q5WQZ9
Pandinuscavimanus Pcascorpine1 73 Active against fungi and bacteria* H2CYP8,[10]
Opisthacanthuscayaporum OcyC7_fragment 53 Active against fungi and bacteria* C5J891, [9]
Hadrurusgertschi HgeScplp1 76 HgeScplp1 has antibacterial activity against B.subtilis. Also it has hemolytic and cytolytic activities on oocytes and erythrocytes.
The short peptide (29-76aa) blocks Kv1.1 (IC50=185 nM) potassium channels. Shows a weak hemolytic activity
Q0GY40, [6,7]
HgeScplp2 84 Active against fungi and bacteria* P0C8W5, [7],
Urodacusyaschenkoi Uroya_1 76 Active against fungi and bacteria* L0G8Z0, [12]
Uroya_2 83 Active against fungi and bacteria*
Vaejovisintrepidus ViScplp1 78 Active against fungi and bacteria* [15]
ViScplp2 78 Active against fungi and bacteria*
ViScplp3 85 Active against fungi and bacteria*
Vaejovispunctatus VpScplp1 78 Active against fungi and bacteria*
Euscorpiopsvalidus Ev37 78 Selectively inhibits Kv1.3 channel (IC50=0.95 µM). P0DL47, [11]
Vaejovismexicanus VmScplp1 77 Active against fungi and bacteria* [15]
VmScplp2 84 Active against fungi and bacteria*
VmScplp3 85 Active against fungi and bacteria*
Vaejovissubscristatus VsScplp1 59 Active against fungi and bacteria*
Tityuscostatus  Tco41.46-2 68 Active against bacteria and may block Kv channels* Q5G8A6,[8]

Table 1: List of scorpine-like peptides obtained from the venom of various scorpions.

single-cell-biology-scorpine-like

Figure 1: Multiple sequence alignment and phylogenetic analysis. A). All known scorpine-like peptipes were aligned with GENEIOUS. Conserved residues on all sequences are in red and conservative replacements are in blue. The N-terminal domain includes residues from 1-43 whereas the residues 44-87 belong to the C-Cαβ domain. Table 1 shows the accession numbers of each sequence to exception of peptides ViScplp1, ViScplp2 and ViScplp3 isolated from Vaejovis intrepidus; VpScplp1 from Vaejovis punctatus; VmScplp1, VmScplp2 and VmScplp3 from Vaejovis mexicanus; VsScplp1 from Vaejovis subcristatus, which were reported in Quintero-Hernández (2015). The percentage of identity was determined with LALIGN v.36.3.5 and the E-value with PSI-BLAST. B). The phylogenetic tree of scorpine-like peptides was constructed with the Neighborjoining algorithm using GENEIOUS Software. Tree shows 22 scorpines grouped in three main clades. First clade similar to Heteroscorpine-1 grouping all the Opiscorpines, Pcascorpine and Panscorpine. Second clade consists of scorpines similar to Hge-scorpine-1, short-chain-scorpine, having a few Vaejovis scorpine-like-peptides and one from Urodacus. The last clade contains largechain- scorpines similar to Hge-scorpine-2 grouping the remaining Vaejovis scorpine-like peptides and the long scorpine from Urodacus.

Antimicrobial and antiviral activity

Other examples of antimicrobial effect were reported. For instance, Opiscorpine showed anti-fungal activity against Fusarium oxysporum, a pathogen causing Fusarium wilt in many plants [13]. HgeScplp1 shows cytolytic activity at 200 nM in oocytes and erythrocytes and also inhibit the growth of B. subtilis at 2 μM [16]. In contrast, the recombinant version of the scorpine-like Ev37 did not show any antimicrobial or hemolytic activity at 20 and 10 μM concentrations [11]. It remains to be shown whether the folding of the recombinant peptide is the correct one, necessary for activity. Scorpine was also demonstrated to affect viral replication in cell culture containing Dengue virus [19].

Antimalarial activity

Scorpine-like peptides have been used to control malaria, in special Scorpine, which has been recombinantly expressed in Anopheles gambie cells showing antibacterial activity against B. subtilis and K. pneumonia, at 5 and 10 μM, respectively. It also produced 98% mortality in sexual stages of Plasmodium berghei (ookinetes) at 15 μM and 100% reduction in P. falciparum parasitemia at 5 μM [19]. Moreover, the overexpression and secretion of Scorpine into the hemolymph from transgenic mosquitoes reduced sporozoite counts by 98% just a few days after a Plasmodium-infected blood meal, suggesting that it could be a powerful weapon for combating malaria [19,20]. Interesting results were obtained by heterologous expression of Scorpine in a strain of Metarhizium anisopliae fungus that can parasite mosquitoes. A fusion protein made with a repeat of peptides [SM1]8 with Scorpine directs the product to the salivary glands of the mosquitoes [20]. Infection with these transgenic fungi reduces 98% of Plasmodium sporozoites in the salivary gland. The expression of those genes shortened the time taken to reduce transmission of malaria compared to wild type M. anisopliae and can reduce transmission rates even when the mosquito has an advanced infection. M. anisopliae can infect A. gambiae, A. arabiensis, A. funestus and other populations of mosquitoes. This transgenic strategy could have a wider impact. It could potentially be used to tackle other diseases spread by mosquitoes and other insects [20].

Modulation of activity of potassium channels

The C-terminal region of HgeScplp1 blocks Kv1.1 channel currents (IC50 88 nM) [16] while the recombinant version Ev37 is able of inhibit the Kv1.3 channel current showing an IC50 value of 1 μM [11].

Mechanism of Action

The mechanism of action of scorpine-like peptides on microbes probably is similar to that described for the scorpion-AMPs. The latest are generally defined by containing 2-9 positively charged lysine or arginine residues plus hydrophobic amino acids. These residues confer physicochemical properties permitting interactions with microbial membranes and enabling their typically broad-spectrum antimicrobial activity by directly disrupting the membrane [21]. The membrane is disrupted in several stages; first the electrostatic interaction of AMPs with the polyanionic surface of lipopolysaccharide on the bacterial membrane causes an increase in surface area that weakens the bilayer. This alteration leads to pore formation causing the cell lyses. The disruption can be explained by diverse models (barrel stave, carpet model or torodial model) but merely forming a pore that creates an aqueous channel in the microbe membrane that leads to the loss of polarization, loss of intracellular cellular contents, disturbance of membrane function from lipid redistribution and finally death [21,22].

In the antimalarial activity both domains may be involved, the cytolytic activity of the N-terminal and the blocking effect of the C-terminal on potassium channels, although there are few studies on the subject but the mechanism of action has not been elucidated [20,23].

Concluding Remarks

Scorpine-like peptides are interesting compounds of scorpion venom that might aid against the fight towards multi-resistant drug bacteria, contribute to the understanding of potassium ion channels, control viral replication and help in combating malaria. The N-terminal region of scorpine-like peptides might provide antimicrobial and antifungal activities, whereas the C-terminal region might provide novel potassium channel modulators. The critical aspect would be to cleave the peptide in such a way to create (1) a potent AMP with the linear region of scorpine-like peptides and on the other hand, (2) a specific ion channel modulator that contains the motifs that provide the bioactivity.

Scorpines have shown promising antimalarial activity. By producing transgenic mosquitoes that express recombinant scorpines, the malaria parasite transmission can be interrupted. This effect is due to the bi-functionality of scorpine-like peptides. However, our understanding of the mechanism of action of scorpine-like peptides towards parasites and viruses remains unclear. All these evidences make scorpine-like peptides attractive compounds for further research and development into drug leads.

Acknowledgements

Partially supported by grants from SEP-CONACyT No. 237864 and DGAPAUNAM IN203416 to LDP.

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Review summary

  1. Junaid Khan
    Posted on Aug 18 2016 at 12:20 pm
    The article presents a great review on a class of poorly studied toxin present in the scorpion venoms. Scorpine-like peptides are bi-functional molecules showing antimicrobial activity (N-terminal region) and the blocking effect on potassium channels (C-terminal region).The review is concise but addresses the main structural and functional aspects known to date on this class of toxins. The paper presents an analysis of the similarities of the primary structures of proteins classified as scorpine-like, highlighting the conserved amino acids, which are probably also important for their functions. The article will help in the development of novel studies aiming at the biotechnological applications of this class of toxins.
 

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