Seasonal Variation in the Pigment Content of Dominant Macrophytes from Wular lake, Kashmir Himalaya, India

Aquatic macrophytes which are considered to be the photosynthetic organisms of freshwater habitats are central to the function of fresh water ecosystems [1]. Macrophytes are a potential source of food and fodder for humans and bovine population, besides serving as a base of aquatic food chain [2-6]. In order to evaluate the food potential of macrophytes and estimation of their nutritional value, the knowledge of their chemical composition is of paramount importance [5,7,8]. It is well known that under the influence of abiotic factors such as light, temperatute, sediment composition and water chemistry, macrophytes can modify their metabolic responses (photosynthesis and growth rates) and levels of proximate constituents [9-12] thus necessitating the determination of biochemical ingredients of the tissues produced under each ecological condition. Light and temperature act together in affecting growth, morphology, photosynthesis, chlorophyll composition and reproduction of macrophytes [13-18]. The biological functions such as photosynthesis, physiology, etc. in macrophytes are dependent on chlorophyll a (chl a), a key photosynthetic pigment. Consequently, changes in chl a can indicate plant growth [19]. or disturbances from stressors [20]. It is believed that the distinct variation in pigment content as well as specific features of the pigment complex ensure adaptation of macrophytes to light conditions in water stratum [17,18,21].

lying between 34°16´24.67˝ -34° 20´26.26˝ N latitudes and 74° 33´41.42˝ -74o 44´02.90˝E longitudes of Kashmir Valley. The oxbow type lake is mono-basined, elliptical in shape and is of fluviatile in origin, formed by the meandering of River Jhelum. Various rivers and streams which contribute their might draining to the Wular lake are River Jhelum, Erin Nallah, Madhumati Nallah, Ferozepur Nallah, Sukhnag Nallah, Ningal Nallah, Ajus Nallah, etc. and the only exit (outlet) of the lake is River Jhelum. The macrophyte dominant lake has well developed littoral zone. Extended littorals in general, are dominated by (i) reeds like Phragmites australis, Typha angustata and Sparganium ramosum, (ii) rooted floating-leaf types like Nymphoides peltatum, Trapa natans and Nymphaea mexicana, and (iii) freefloating types dominated by Salvinia natans, Azolla sp. and Lemnids, all growing together intermixed and forming a complex physiognomy. The slightly deeper zone has abundant growth of submergeds like Ceratophyllum demersum, Potamogeton crispus and Potamogeton lucens forming meadows at various places. The littorals towards the north-eastern side are densely populated with Salix plantation which is eating the vitals of the lake.

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Maximum temperature was recorded in summer (25.3 ± 7.9°C) and minimum in autumn (14.4 ± 7.3°C). Ammonia and Total Phosphorus exhibit same seasonal patterns with autumn depicting higher values (123 ± 46.7 and 194 ± 61.1) and summer depicting lower values (69 ± 32.7 and 124 ± 53.9), respectively. The seasonal mean values of nitrate in the present investigation varied between 299.8 ± 179.7 µg/l and 482.6 ± 214.8 µg/l in summer and spring respectively. In contrast maximum transparency values (0.9 ± 0.3 m) were obtained in autumn against a minimum (0.5 ± 0.1 m) being recorded in spring (Table 1).

Seasonal variation in pigment content
Perusal of data on the pigment content of macrophytes revealed significant difference for Chlorophyll-a, Chlorophyll-b and Total Chlorophyll in the different groups of macrophytes collected from Wular lake. The levels of Chlorophyll-a, the primary photosynthetic pigment in macrophytes in the present study varied from 1.5 ± 0.1 µg/ml to 3.7 ± 0.5 µg/ml. The values were highest in Ceratophyllum demersum (3.7 ± 0.5 µg/ml) and lowest in Azolla sp. (1.5 ± 0.1µg/ml). The Chlorophyll-b content recorded its highest value in Ceratophyllum demersum where the data observed (1.7 ± 0.5 µg/ml) was succeeded by Potamogeton crispus with a value of (1.6 ± 0.5 µg/ml). The lowest Chlorophyll-b values were obtained in Azolla sp. having a value of (0.3 ± 0.2 µg/ml).The Total Chlorophyll content also recorded its highest value in Ceratophyllum demersum (4.1 ± 0.5 µg/ml) followed by Lemna minor with a value of 4.0 ± 0.7 µg/ml, decreasing to a lowest of 2.1 ± 0.9 µg/ml in Azolla sp. ( Table 2).
The macrophytes collected during different seasons exhibited distinct seasonal profile in the pigment content. Significant changes were seen in all the macrophytes except that of Ceratophyllum demersum and Potamogeton crispus where slight fluctuations were noticed. In the present work, maximum pigment content was observed during summer season, the period of active growth characterized by optimum nutrient load and temperature. The lowest levels of Chlorophyll-a and Chlorophyll-b were obtained during the period of less vigorous growth in autumn, and they increased considerably during the period of sprouting and early growth stages of macrophytes in spring and were highest in summer. However, relative content of Total Chlorophyll in the macrophytes under study was notably lower in spring season than in autumn (Figures 1-4).
In general submerged macrophytes represented in the present study by Ceratophyllum demersum and Potamogeton crispus were characterised by high pigment content.While as lower pigment levels were obtained in emergents and rooted-floatings. Chlorophyll-a and Chlorophyll -b and Total Chlorophyll showed highly significant positive correlation (r=0.96, 1.00 and 0.95, P<0.05) with water temperature and negatively correlated with nutrient salts such as nitrate, ammonia and total phosphorus. However, transparency showed a non-significant positive correlation (r=0.23, P=0.05) with Total Chlorophyll and and negative correlation with Chlorophyll-a and Chlorophyll-b (Table 3).

Discussion
The analysis of our data revealed some distinct trends in the pigment content in the fresh water macrophytes sampled during different seasons. In the present work, maximum pigment content of the macrophytes was observed during summer season; the period characterized by optimum nutrient load and water temperature.
Chlorophyll-a and Chlorophyll-b contents were significantly lower in autumn, Total chlorophyll was the only exception. The trend may be attributed to the positive role of water temperature in promoting locations within the Wular lake, in order to determine the chlorophyll content of these macrophytes ( Figure 1). These aquatic plants were harvested in a lush -green condition using quadrants of 0.25 m 2 in an area [22,23] from different areas within each stand to form three representative samples for each species. The harvested materials were placed in polyethylene bags and transported to the laboratory in cool boxes (temperature 4°C) where they were incised to include only leaves. These were then washed thoroughly and moisture was drained before being analyzed for chlorophyll content.
Chlorophyll content (Chl a, Chl b and Total Chlorophyll) of macrophytes was determined Spectrophoto-metrically, in acetone extracts prepared from fresh samples using standard methods [24,25]. Acetone extract of the macrophytes was prepared by macerating 1 g fresh weight of plant leaves with 80% aqueous acetone using a mortar and pestle. The decanted suspension was centrifuged for 3 minutes at 1320 r.p.m. After centrifugation, the upper green clear solution was decanted from the colorless residue and then made up to 10ml with 80% acetone in 10 ml test tubes. The solution was then subjected to centrifugation at 10,000 r.p.m for 10 minutes. The absorbance of the solution was determined using a spectrophotometer (Systronics-116) at wavelengths of 665 and 649 nm respectively. The results were expressed as µg/ml.

Analysis of hydrological parameters
Surface water samples were collected from all the selected stations in clean TARSON bottles for analysis of hydrological parameters like surface water temperature, transparency, nitrate, ammonia and phosphate on seasonal basis. The temperature of each water sample were measured at the field by means of a mercury thermometer, while as transparency in water was measured with Secchi disc. Other water quality parameters such as nitrate, ammonia and phosphate, which require photometric measurements, were done according to the standard methodology outlined in APHA [26] by using model spectrophotometer (Systronics-116) on the same day in Aquatic Ecology Laboratory, Department of Environmental Science, University of Kashmir-190006, India.

Statistical analysis
All the data were expressed in terms of mean ± S.D (standard deviation) and range. In addition, Pearson correlation coefficient (r) was also computed between the pigment content of the macrophytes and environmental parameters. Descriptive statistics were calculated using StatSoft Statistica 7.0 statistics software.     an increase in the pigment content in their chloroplast. Our results further gain support from the findings of [17,32] who also reported higher pigment content in submerged aquatic plants. The free floating macrophytes represented in our study by Azolla sp. and Lemna minor contain higher pigment levels in contrast to emergent and rootedfloating macrophytes is due to their presence of homogenous type of mesophyll structure [14] [17] suggested that within individual plants, total chlorophyll can vary significantly in response to light, with greatest chlorophyll concentration at the basal (darkest) portion of the plant. The normal life activity is ensured by low pigment content in plants adapted to high radiation. One of the advantages in having the low pigment content is that it prevents cell damage caused by photooxidation [17]. This regularity was observed for desert and highmountain plants existing under conditions of high light intensity [31].

Conclusion
It is evident from the study that different groups of macrophytes showed distinct profile in their seasonal pigment content. The results obtained in our study establish that physico-chemical parameters have profound influence on the pigment content of aquatic plants.
Significant correlation values computed among the environmental parameters and pigment content suggest that the abiotic parameters seem to have potential role in photosynthetic pathways of macrophytes.
greater chlorophyll concentration and photosynthesis [13][14][15][16]. The summer fall in the amounts of nutrients (nitrate, ammonia and total phosphorus) is attributed to the greater photosynthetic activity of macrophytes especially submergeds leaving very small quantities of such nutrients in water [27]. This is confirmed from the significant positive correlation between Chlorophyll-a, Chlorophyll -b and Total Chlorophyll and water temperature and negative correlation with nutrients (nitrate, ammonia and total phosphorus).The lower quantity of chlorophyll pigment in macrophytes in other seasons reflects their adaptation to decreased water temperature [28]. Our results are in accordance with the published data [29,30]. Thus the active period of pigment synthesis in macrophytes coincides with the increase in water temperature and decrease in nutrient load.
The macrophytes as a whole were characterized by low chlorophyll pigment content per fresh weight basis in comparison to the terrestrial plants of middle latitudes [31]. The comparative analysis of data ( Table 2) showed appreciable variations in the pigment content among different groups of macrophytes with different degree of leaf submergence (emergent, floating, free floating and submerged). In emergent and rooted-floating macrophytes, the low chlorophyll pigment content might be attributed to their existence at high irradiance [17]. In rooted-floating macrophytes the leaves are perpendicular to incident radiation. As a consequence of which they contain lower pigment content compared to submerged aquatic plants. In submerged macrophytes, the leaves are almost vertically oriented [17]. As a result, they do not shade each other resulting in higher pigment content in them. In the submerged leaves, in contrast to the emergent and floating ones, an increase in the photosynthetic rate of a single chloroplast as well as their existence in water under low light conditions results in