alexa Serologic Investigation of Pneumococcal Immunization of Autologous Hematopoietic Stem Cell Recipients | OMICS International
ISSN: 2327-5073
Clinical Microbiology: Open Access
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Serologic Investigation of Pneumococcal Immunization of Autologous Hematopoietic Stem Cell Recipients

Joshua Lukenbill1*, Sherif Mossad2, Robert Butler3, Ronald Sobecks1, Edward Copelan4, Matt Kalaycio1, Steven Andresen1, Robert Dean1, Brad Pohlman1 and Brian Bolwell1

1Department of Hematologic Oncology and Blood Disorders, Taussig Cancer Institute, Cleveland, Ohio, USA

2Department of Infectious Disease, Medicine Institute, Cleveland Clinic, Cleveland, Ohio, USA

3Department of Quantitative Health Sciences, Cleveland Clinic, Cleveland, Ohio, USA

4Department of Hematologic Oncology and Blood Disorders, Levine Cancer Institute, Charlotte, North Carolina, USA

*Corresponding Author:
Joshua Lukenbill
John T Vucurevich Cancer Care Institute
Rapid City Regional Health
353 Fairmont Blvd, Rapid City
South Dakota, USA
Tel: 605-719-5276
Fax: 605-719-4768
E-mail: [email protected]

Received date: September 09, 2014; Accepted date: October 29, 2014; Published date: October 31, 2014

Citation: Lukenbill J, Mossad S, Butler R, Sobecks R, Copelan E, et al. (2014) Serologic Investigation of Pneumococcal Immunization of Autologous Hematopoietic Stem Cell Recipients . Clin Microbiol 3:177. doi: 10.4172/2327-5073.1000177

Copyright: © 2014 Lukenbill J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Clinical Microbiology: Open Access


Our goal was to prospectively assess the immunological response to the 23-valent pneumococcal polysaccharide vaccine (PPSV23) in autologous cell transplantation (AHCT) recipients using pneumococcal IgG (PNEIGG) titers. PNEIGG titers were collected prior to transplant (titer 1), 3-7 days following AHCT (titer 2), 1 year later at the time of vaccination (titer 3), and at 4-6 wks after vaccination (titer 4) for each of the 23 serotypes included in PPSV23. Seroreactivity was defined as a =4-fold increase in titer or a titer of >1.3 µg/mL following vaccination. Only 2 of 10 AHCT recipients with data through titer 4 were seroreactive in the majority of the 23 pneumococcal serotypes following PPSV23 which were not prior to vaccination. Median titer concentration increase reached the threshold for seroreactivity only for serotypes 12F and 15B. Antibody response of the 5 serotypes analyzed (4,8,12F,14,19F) was significantly lower in the AHCT population compared to healthy historical controls. There appears to be minimal immunogenicity as measured by PNEIGG titers to PPSV23 one year following AHCT.


Autologous hematopoietic cell transplantation (AHCT) recipients are vulnerable to many infections, including Streptococcus pneumonia, given the inherent immunosuppression associated with this procedure. Kumar et al. analyzed 1238 patients following HCT, of whom 9 of 431 (2.1%) allogeneic and 5 of 807 (0.06%) autologous recipients developed invasive pneumococcal disease [1]. Therefore, primary prevention with vaccination is paramount.

This prospective study’s goal was to provide further insight into the immune response to the 23-valent polysaccharide vaccine (PPSV23, Pneumovax 23, Merck & Co., Whitehouse Station, NJ) in AHCT recipients. This was assessed by measurement of seroreactivity following vaccination, as quantified by pneumococcal IgG (PNEIGG) antibody titers. Of note, this study was designed and conducted to allow for PPSV23 at 1 year post-HSCT, as per the 2000 Centers for Disease Control and Prevention/Infectious Disease Society of America/American Society for Blood and Marrow Transplantation recommendations [2]. The primary outcome was PNEIGG at 4-6 weeks after PPSV23. We also collected PNEIGG titers at 3 other time points: before AHCT, following stem cell infusion, and 1 year later at the time of vaccination. Finally, we compared our AHCT population’s PNEIGG response following vaccination to that of historical healthy controls.

Materials and Methods

Fifty patients with lymphoma and multiple myeloma undergoing AHCT at the Cleveland Clinic between June 2009 and March 2010 were recruited. Patients were treated on AHCT protocols that had previously been approved by the Cleveland Clinic’s Institutional Review Board with signed informed consent obtained from all patients. In addition, written informed consent for participation in this study was obtained from all subjects. Historic control data regarding the immunologic response to PPSV23 in healthy elderly persons was extracted from a study by Carson et al. [3]. This study was designed to analyze the differential immunological response of young and healthy persons to PPSV23. PNEIGG data were collected pre-vaccination and then 4 weeks after vaccination for five serotypes (4, 8, 12F, 14, 19F). The PNEIGG results from both arms in that study were combined for the 50 controls then compared to out AHCT study population for those 5 serotypes.

Titer 1 was collected during pre-transplant evaluation, prior to hospitalization for AHCT. Titer 2 was collected 3-7 days following stem cell infusion. Titer 3 was collected at the time of pneumococcal vaccination 1 year following AHCT. Finally, Titer 4 was collected 4-6 weeks after vaccination. These samples were then allowed to coagulate at room temperature for 30 minutes, followed by a 10 minute 1300 g centrifuge run at 4°C. The serum supernatant was then aliquoted into two 1.8 mL cryovials then immediately frozen at -80°C. These samples were sent for PNEIGG measurement (Mayo Medical Laboratories, Rochester, MN). At 1 year post-AHCT, participants were offered PPSV23. There were no unexpected complications of vaccination in any of the subjects.

The sample size calculation was based on data from Mufson et al. on pneumococcal antibody titers following vaccination [4]. In order to achieve a power of 80% and an alpha of .05, a sample size of 42 was required (mean of the differences=1.1, population standard deviation=2.7); therefore, our goal accrual was 50 subjects. For each of the 23 serotypes analyzed, seroreactivity was defined as a titer >1.3 µg/mL, or a fourfold increase in titer 3 (pre-vaccination) to titer 4 (post-vaccination) [5]. Two way ANOVA methods using subjects and titers to adjust for repeated measures within a subject were used to assess differences in titer results. Contrasts were constructed to test specific pair-wise means (baseline vs. initial, baseline vs. 1 year later, 1 year later vs. 1.1 years later, and post-vaccination). One sample equivalence-test was used to compare antibody titers of AHCT recipients 4-6 weeks post-vaccination to historical healthy controls. Assessment of differences between the data by Carson et al. [3] and our study’s titers 3 and 4 were run using one sample t-tests with derived target null values. Results were not adjusted for multiple comparisons. Differences with p<.05 were considered to be statistically significant.


Fifty patients were enrolled to participate in the study. However, 40 patients dropped out prior to completion of the study, most commonly due to continuation of care at a community oncology clinic (24 patients). Of the remaining 10 patients, the median age was 56, 9 were male, 6 had multiple myeloma, and 4 had non-Hodgkin lymphoma. The median number of previous chemotherapy regimens was six, one had prior AHCT, one had previous radiotherapy, one with previous pneumococcal vaccination, and one had previously documented pneumonia.

Only two patients acquired seroreactivity to a majority of serotypes in response to vaccination (comparison of Titer 3 to 4). Four patients maintained the significant seroreactivity that they had prior to vaccination. Finally, four patients had minimal seroreactivity, both prior and following vaccination. Analysis of the median change in titer concentrations revealed minimal variation at the three intervals as well. Only serotypes 12F and 15B had a median titer increase from titer 3 to titer 4 which reached the threshold for seroreactivity. Finally, our data were compared to the healthy historical controls in the study by Carson et al. [3], revealing significantly different mean serotype responses to PPSV23 to all 5 compared serotypes (p<.0001), as shown in the Table 1. All five serotypes (4, 8, 12F, 14, 19F) in the control data achieved the definition of seroreactivity, but only serotype 12F (as well as 15B) were seroreactive in our study population.

  Current Study Carson et al*
Titer Pre-vaccination# Post-vaccination^ Pre-vaccination Post-vaccination
4 0.789 1.23 6.19 21.4
8 1.61 2.3 1.89 16.8
12F 1.11 3.53 7.3 26
14 2.3 4.17 133 318
19F 6.36 7.05 75 242

Table 1: Comparison of mean titer concentrations† of AHCT recipients to healthy controls*


This study revealed minimal immunogenicity to PPSV23, as measured by number of subjects with seroreactivity in the majority of titers, the number of serotypes with seroreactivity across all subjects, and in comparison with historic controls. The primary limitation of this study is that it failed to reach the goal sample size of 42 patients. Patients’ dropout was attributable to may causes, including progressive disease, co-morbidities, and death; however, the primary reason was lack of patient follow-up. This lack of data for Titers #3 and 4 compromises the external validity of our results.

The AHCT population has not been well-studied; however, this trend of poor immunogenicity to PPSV23 appears to extend to all of the hematopoietic stem cell transplant population. A study by Kumar et al. including autologous and allogeneic transplant patients, found that the serotypic response to PPSV23 was inferior to the conjugate vaccine PCV7 (Prevnar, Wyeth Pharmaceuticals Inc., Philadelphia, PA) at 12 months post-transplant (p=.003) [6]. The Advisory Committee on Immunization Practices recently endorsed PCV13 (PCV13, Prevnar 13, Wyeth Pharmaceuticals Inc., Philadelphia, PA) as the preferred vaccine in adults =19 [7]. However, administration of PPSV23 after the series of 3 doses of PCV13 has been shown not only to increase serotype coverage but also to increase the immunogenicity to serotypes included in the conjugate vaccine [8]. Therefore, the current recommendation for all hematopoietic cell transplant recipients is to receive a series of 3 administrations of the PCV13 vaccine, followed by the PPSV23 vaccine 8 weeks later [7,9].

The primary goal of this study was to assess the immunologic response to PPSV23 in patients undergoing AHCT. Though our sample size was limited, our data support that PPSV23 vaccination alone induces minimal immunogenicity as measured by PNEIGG. It is our hope that this study and others on the topic will increase our knowledge of the efficacy of pneumococcal vaccination in AHCT recipients.


Supportive foundations are National Institutes of Health, National Center for Research Resources, CTSA 1UL1RR024989, Cleveland, Ohio.


Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Relevant Topics

Recommended Conferences

Article Usage

  • Total views: 11797
  • [From(publication date):
    December-2014 - May 21, 2018]
  • Breakdown by view type
  • HTML page views : 8009
  • PDF downloads : 3788

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7