|Maekawa T1*, Masayuki Ida1, Yuichi Furukawa1, Yoshinori Kitagawa1, Kiyoshi Yasui2, Yasuyuki Kowata3, Takayuki Izumo1 and Hiroshi Shibata1|
|1Institute for Health Care Science, Suntory Wellness Limited, 8-1-1 Seikadai, Seika-cho, Soraku-gun, Kyoto 619-0284, Japan|
|2Yotsubashi Clinic, 1-22-9, Shinmachi, Nishi-ku, Osaka-shi, Osaka, Japan|
|3Ueno Clinic, Aisei Hospital, 2-18-6, Higashi-Ueno, Taito-ku, Tokyo, Japan|
|Corresponding Author :||Maekawa T
Institute for Health Care Science
Suntory Wellness Limited, 8-1-1 Seikadai
Seika-cho, Soraku-gun, Kyoto 619-0284, Japan
E-mail: [email protected]
|Received: November 27, 2015 Accepted: December 23, 2015 Published: December 28, 2015|
|Citation: Maekawa T, Ida M, Furukawa Y, Kitagawa Y, Yasui K, et al. (2015) Supplementation with Lactobacillus pentosus strain S-PT84 and Vitamin B Mixture Enhances Natural Killer Cell Activity in Healthy Humans. J Prob Health 4:134. doi:10.4172/2329-8901.1000134|
|Copyright: © 2015 Maekawa T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Background: The maintenance of natural killer cell (NK) activity is critical for health, because NK cells target infected cells and cancer cells. In this study, we investigated the effect on human NK activity of supplementation with a combination of S-PT84 and vitamin B mixture (VBM: vitamin B1 (thiamin), vitamin B2 (riboflavin), and vitamin B6 (pyridoxine)).
Methods: We designed randomized, placebo-controlled, double-blind, parallel-group comparative studies. In the first study, we recruited healthy middle-aged (30- to 69-year-old) subjects who had low NK activity (Study 1). Subjects received a combination of S-PT84 (1.5 × 109 cells) and VBM or placebo supplement (derived from dextrin) for 4 weeks, with a 4-week follow-up phase. In the second study, healthy middle-aged (40- to 69-year-old) subjects received a combination of S-PT84 and VBM or placebo supplement for 12 weeks (Study 2). In both studies, we measured NK activity in peripheral blood mononuclear cells using the 51Cr-release assay, and assessed safety by comprehensive analysis of blood hematology, serum biochemistry, urinalysis, and physical state.
Results: In Study 1, supplementation with 1.5 × 109 cells of S-PT84 and VBM enhanced NK activity during the dosing interval; NK activity subsequently returned to baseline during the wash-out period. In Study 2, supplementation with S-PT84 and VBM enhanced NK activity during the 12 weeks of ingestion. The increase of NK activity in the placebo group (r=-0.193, p<0.01) and S-PT84-VBM group (r=-0.352, p<0.01) was inversely correlated with NK activity at baseline, respectively. Moreover, the regression coefficient of S-PT84-VBM’s regression line was smaller than that of placebo’s significantly (t=2.14, p=0.03). No adverse effects were observed in either study.
Conclusions: These results suggest that daily supplementation with 1.5 × 109 cells of S-PT84 and VBM enhances NK activity in humans, even during long-term (12-week) administration. Therefore, our results indicate that S-PT84 and vitamin B supplementation may promote healthy state.
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