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ISSN: 2157-7439
Journal of Nanomedicine & Nanotechnology
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Surface Coatings Protect against the In vitro Toxicity of Zinc Oxide Nanoparticles in Human Hepatic Stellate Cells

Megan J Osmond-McLeod1*, Yalchin Oytam1,2, Ronald IW Osmond3, Fariborz Sobhanmanesh2 and Maxine J McCall1

1CSIRO Advanced Materials TCP (Nanosafety), and CSIRO Food and Nutrition Flagship, North Ryde NSW 1670, Australia

2CSIRO Genomics and Cellular Sciences, Transformational Biology CP, North Ryde NSW 1670, Australia

3TGR Biosciences, Thebarton SA 5031, Australia

*Corresponding Author:
Megan Osmond-McLeod
CSIRO Food and Nutrition Flagship, PO Box 52
North Ryde, NSW, 1670, Australia
Tel: 612-94905035
Fax: 612-94905010
E-mail: [email protected]

Received Date: August 26, 2014; Accepted Date: September 25, 2014; Published Date: October 05, 2014

Citation: Osmond-McLeod MJ, Oytam Y, Osmond RIW, Sobhanmanesh F, McCall MJ (2014) Surface Coatings Protect against the In vitro Toxicity of Zinc Oxide Nanoparticles in Human Hepatic Stellate Cells. J Nanomed Nanotechnol 5:232. doi:10.4172/2157-7439.1000232

Copyright:© 2014 Osmond-McLeod MJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Coatings applied to the surfaces of zinc oxide (ZnO) nanoparticles have been shown to decrease the capacity of uncoated nanoparticles to generate free radicals, as well as to reduce their solubility, and may be utilized in safetyby- design approaches to decrease ZnO nanoparticle toxicity. The liver is a target organ for ZnO nanoparticles, or ionic zinc released from ZnO nanoparticles, whether the route of entry is by dermal absorption, ingestion, injection or inhalation. Liver injury and fibrosis have been demonstrated in vivo in response to ZnO nanoparticle treatment, and cell injury has also been shown in vitro using immortalized hepatocyte cell lines. Despite their activation being a critical step for liver fibrosis, however, the response of human hepatic stellate cells (hHSCs) to ZnO nanoparticles has not yet been reported. Here, hHSCs were treated with four types of commercially-available ZnO nanoparticles suspended in cell culture medium. Two types were uncoated nanoparticles of different sizes, and the other two types with similar particle size carried different surface coatings. Cells were also treated with pre-dissolved ZnSO4 to provide a comparison of the cellular responses induced by ionic zinc. A systems biology approach was utilized whereby the activation of cell signaling pathways, changes in the transcriptome, and alterations in cellular function were assessed over a 24 h period. Treatment with both types of uncoated ZnO nanoparticles mobilized pathways and responses centered on cellular stress, survival, and apoptosis. Both surface coatings conferred almost complete protection against ZnO nanoparticle-induced cytotoxicity. Ionic zinc had little effect on these cells indicating that toxicity is mainly induced by the nanoparticles. The results presented here support the use of surface coatings in commercial applications of ZnO nanoparticles where there is human exposure.

Keywords

Zinc oxide nanoparticle; Human hepatic stellate; Nanoparticle coating; Gene expression; Cell-signaling

Introduction

The unique catalytic, UV-absorbing, electronic, and optical properties of zinc oxide (ZnO) nanoparticles have been exploited throughout a range of technological innovations, some of which may result in direct consumer contact. For example, their inclusion in personal-care products, biomedical and antimicrobial applications, and food and agricultural technologies [1-5], may result in nanoparticle entry, both intended and unintended, into the human body. Therefore, the biodistribution, fate, and impact of ZnO nanoparticles in vivo, as well as their impact on various cell populations In vitro, are important to assess. In vitro studies have shown that ZnO nanoparticles can exert oxidative, genotoxic, and cytotoxic effects across a range of cell types [6]. Although other mechanisms may contribute [7-9], the predominant cause of ZnO nanoparticle toxicity has been ascribed to the release of ionic zinc [10,11]. The presence of a stable surface coating has previously been shown to exert a protective effect on ZnO nanoparticle cytotoxicity in primary human olfactory cells [12], potentially via more than one mechanism, including a reduction in free radical generation [13,14], retarding, or preventing, particle dissolution [15], or rendering the particles less available for cellular uptake.

The liver has been shown to be a target organ for the accumulation and/or biological impact of ZnO nanoparticles, or Zn2+ released from ZnO nanoparticles. This is so, regardless of whether the route of entry is by dermal absorption [16], ingestion [17-21], injection [17], or inhalation [22]. Hepatocytes are the predominant cell type in the liver, contributing approximately 80% to its mass, as well as much of its function, and their response to In vitro treatment with ZnO nanoparticles has been investigated elsewhere using immortalised cell lines [9,23-28]. A critical stage in the fibrotic response of the liver to injury, however, is the activation of hepatic stellate cells [29,30], which also function in retinoid storage and transport, immunoregulation, secretion of growth factors, lipoproteins and cytokines, and metabolism and detoxification [30]. Liver injury and fibrosis have been reported outcomes in vivo in rodents following ingestion [18,20,21] and inhalation [22] of ZnO nanoparticles, suggesting that the response of hepatic stellate cells to ZnO nanoparticles is highly relevant.

Here, we examined the response of primary human hepatic stellate cells (hHSCs) to treatment with four types of ZnO nanoparticles that are available commercially, and are used in personal care products such as sunscreens, as well as zinc sulphate (ZnSO4), which was included as a source of ionic zinc. Two types of ZnO nanoparticles were coated, and two were uncoated. A systems biology approach incorporating assessments of cell function and viability, the activations of cell signalling pathways, and whole genome transcriptional profiling, was utilized. The profiles of cells treated with either of the two types of uncoated ZnO nanoparticles were consistent with an initial activation of cellular stress and protection responses, followed by disregulation of the transcriptome, and induction of apoptosis. The presence of stable surface coatings, on the other hand, mitigated the cytotoxicity of ZnO nanoparticles, rendering them relatively benign. Ionic zinc was not found to elicit a similar stress response to that induced by uncoated ZnO nanoparticles in hHSCs under these conditions.

Materials and Methods

Treatments used in this study

Cells were treated with one of four types of ZnO nanoparticles, ZnSO4 (Sigma, Cat# 96495), or were untreated. Two types of the ZnO nanoparticles were uncoated [Z-COTE (BASF, Batch# EHDA3001), and Nanosun P99/30 (Micronisers, Batch# 4051)]; and two were coated [Z-COTE HP1 (BASF, Batch# CNHE0602), coated with triethoxycaprylylsilane, and Z-COTE MAX (BASF, Batch# FCHE1301), coated with a dimethoxydiphenylsilane/triethoxycaprylylsilane crosspolymer].

Physicochemical characterization of nanoparticles

The methods used for extensive physicochemical characterization of the nanoparticles as powders, or as dispersions in H2O or saline, have been described elsewhere [12]. Briefly, primary particle size, size distribution, and shape were assessed by transmission electron microscopy (TEM) using a Tecnai 12 TEM (FEI, Eindhoven, Netherlands) operating at 120kV under a variety of magnifications. Specific surface areas were determined using the Brunauer-Emmett-Teller (BET) gasadsorption technique [31]. The ZnO nanoparticles were determined to be free of endotoxin using a QLC-1000 Chromogenic LAL kit (Lonza). Levels of peroxynitrile and superoxide radicals generated by the particles dispersed in saline solution were measured under light and dark conditions using electron paramagnetic resonance (EPR), with TEMPONE-H (Enzo Life Sciences, Australia) as the spin trap. Dynamic light scattering (DLS) and zeta potential measurements were taken in H2O at 0 h using a Zetasizer Nano Series (Malvern Instruments, ATA Scientific, Australia), to assess average hydrodynamic particle size and surface charge, respectively. In addition, hydrodynamic particle size distributions in H2O at 0 h were measured by differential centrifugal sedimentation (DCS) (CPS DC24000 UHR Disc Centrifuge, LPS Instruments, Inc.).

For this study, dispersions of nanoparticles (30 μg/mL) in supplemented cell culture medium (HM-5201, ScienCellTM Research Laboratories, USA) were also characterized for particle size and surface charge. DLS, zeta potential, and DCS measurements were taken at 0, 2, 6 and 24 h. A calibrated pH meter (Waterproof pHTestr2, Oakton Instruments) was used to measure the pH of the cell-culture medium containing nanoparticles, ZnSO4, or no treatments, at 0, 2, 6 and 24 h.

Cell cultures

Primary human hepatic stellate cells at passage 5 (p5) were propagated as adherent monolayers in complete hepatocyte medium (HM-5201, ScienCellTM Research Laboratories). Cells were propagated from an original culture of primary human hepatocyte cells (HH-5200, ScienCellTM Research Laboratories), and the hepatic stellate cell type was confirmed by morphology. All incubations were performed at 37°C, 10% CO2.

Preparation of experimental suspensions

In vitro experiments were performed as traditional suspension exposures. ZnO nanoparticle powders, or ZnSO4, were weighed into clean, sterile 50 mL Falcon tubes, and cell-culture medium was added to make stock suspensions (1 mg/mL). The suspensions were briefly vortexed, then bath sonicated for 10 min to disperse the nanoparticles. Appropriate test concentrations were prepared by diluting the stock suspension with medium, followed by bath sonication for a further 10 min just prior to use.

Selection of experimental test concentration

Cells were seeded (10,000 cells/well) into clear 96-well plates and incubated overnight. Medium was removed and replaced in quadruplicate wells with medium containing 10, 20, 30, 40, 50, 60, 70, or 80 μg/mL nanoparticles, medium containing no nanoparticles (negative control), or medium containing 5% dimethylsulfoxide (DMSO) (positive control), and incubated. Metabolic activity was measured at 24 h using the MTS assay (20 μL; CellTiter AQueous MTS assay, Promega), with absorbance recorded using a SoftMax® Pro5 microplate reader (Molecular Devices, USA). Signal values from wells containing medium alone, or from matched concentrations of nanoparticles incubated and assayed in the absence of cells, were used to correct for background in experimental wells. A loss of cell viability was inferred from a decrease in metabolic activity in treated cells compared to untreated cells.

Cell signaling assays

The AlphaScreen SureFire platform (PerkinElmer) was used to measure the phosphorylation (p) of key cell signalling proteins representing five canonical signalling pathways. The specific proteins examined were NFκB p65 (p-Ser536), representing the NFκB pathway, Chk1 (p-Ser), and Chk2 (p-Ser45), representing the ATM/ATR pathway, p70 S6K (p-Thr229), representing the mTOR pathway, Akt (p-Ser473) and BAD (p-Ser112), representing the Akt pathway, and MEK1 (p-Thr217/Tyr221), ERK (p-Thr202/Tyr204), p38 (p-Thr185/Tyr187), c-JUN (p-Ser63/Ser73), and JNK (p-Thr185/Tyr187), representing the MAPK pathwaysMAPK pathwayss. Control agonists for JNK and NFκB (20 ng/mL TNFα), Chk1 (10 μM doxorubicin), and Chk2 (100 nM hydroxyurea) were used to treat cells for 15, 90, and 120 min, respectively, to determine whether these proteins were phosphorylated at detectable levels. Cells at p5 were seeded (80,000 cells/well) into 12-well plates and cultured overnight. Medium was removed and replaced with medium containing 30 μg/mL nanoparticles, 30 μg/mL dissolved ZnSO4, or medium containing no treatment, for 0.5, 1, 2, 4, 6, 8, 12, and 24 h. Four replicate wells were used for each treatment. At the appropriate time-points, treatments were removed from the wells, and the cells were lysed with 500 mL 1X AlphaScreen SureFire Lysis buffer, and the lysates were frozen at -80°C until further analysis. An Envision multilabel plate reader (PerkinElmer) was used to measure well signals, using standard Alpha settings.

Cell stress and viability assays

A number of plate-based assays were used to assess cellular stress and function in response to nanoparticle exposure as previously described [12], with the following modifications: cells at p5 were seeded (10,000 cells/well) into 96-well plates and incubated overnight, after which medium was removed and replaced with medium containing 30 μg/mL nanoparticles, 30 μg/mL ZnSO4, or medium containing no treatment, and incubated for 6 or 24 h. Three to four replicate wells for each treatment were used for each assay. Signal background was determined as described above.

The following cellular responses were assessed: NAD(P) H-dependent dehydrogenase activity (CellTiter96®AQueous One Solution proliferation assay, Promega), redox enzyme activity (CellTitre-BlueTM cell viability assay, Promega), ATP levels (CellTiterGloTM luminescent cell viability assay), lactate dehydrogenase (LDH) activity (CytoTox-ONE homogenous membrane integrity assay, Promega), total glutathione (GSH) (GSH-Glo glutathione assay, Promega), chymotrypsin-like and caspase-like proteasome activities (Proteasome-Glo cell-based assays, Promega), and caspase 3-7 activity (Apo-ONE homogenous caspase-3/7 assay, Promega). Absorbance/luminescent/fluorescent signals in wells were measured using a SoftMax® Pro5 microplate reader (Molecular Devices, USA).

Statistical analyses for cell viability and cell signalling assays

Time-course responses for cell viability and cell signalling assays were analyzed using GraphPad Prism 5.01 (GraphPad Software, USA). Treatment-related differences over time were assessed for statistical significance by 2-way ANOVA with Bonferroni’s multiple comparisons test, where statistical significance was set at p<0.05.

Whole genome transcriptional profiling by microarray

Cells at p5 were seeded (120,000 cells/well) into 6-well plates and cultured overnight. Medium was removed and replaced for 24 h with medium containing 30 μg/mL nanoparticles, 30 μg/mL ZnSO4, or medium containing no treatment. Two replicate wells were used for each treatment. RNA was isolated and assessed for integrity as previously described [12]. A total of 12 samples were prepared as one batch for microarray analysis using the Affymetrix GeneChip® Human Genome U133 Plus 2 and GeneChip® 3’ IVT PLUS Reagent kits (both from Millennium Science, Australia) containing arrays and reagents, and following manufacturer’s instructions. The microchips were prepared for hybridisation and scanning using an Affymetrix GeneChip® Hybridisation, Wash and Stain Kit (Millennium Science), hybridised (16 h, 45°C, 60 rpm in an Affymetrix 640 GeneChip® Hybridization Oven), and then washed (Affymetrix GeneChip® Fluidics Station 450) and scanned ( Affymetrix 7G GeneChip® Scanner). Data from all microarrays passed the associated quality control procedures recommended by Affymetrix.

The gene expression data were normalized by applying the robust multi-array average (RMA) algorithm using Matlab 7.7.0 (The Mathworks Inc., Natick, MA, USA). For each treatment, differentially expressed genes were identified via paired comparisons with the control group followed by correction for multiple tests as previously described [12], but with a more stringent false discovery rate (FDR) of 5%, which was applied here in order to increase confidence in datasets that were generated from replicate microarrays. Final datasets were analyzed using the Ingenuity Pathways Analysis (IPA, Ingenuity® System, www. ingenuity.com)

Microarray data are freely available on the GEO Archive (https:// www.ncbi.nlm.nih.gov/geo/) under accession number GSE60159.

Results and Discussion

To examine whether the presence of surface coatings could protect cells against ZnO nanoparticle cytotoxicity, primary human liver stellate cells were treated with four types of ZnO nanoparticles - two with surface coatings, and two uncoated. Cells were also treated with pre-dissolved ZnSO4, which was included to provide a comparison of cellular responses to ionic zinc. The test concentration selected for these experiments (30 μg/mL) had elicited a range of treatment-dependent differences in cell viability in an initial 24 h concentration-response assay (Figure 1), and was therefore anticipated to be sufficient to induce a measurable response across a range of earlier time-points up to 24 h. A systems biology approach was taken whereby the cellular responses to treatment were assessed using a variety of cell-based stress and viability assays (measuring markers for antioxidant capacity, proteasome activity, metabolic activity, membrane integrity, and apoptosis), cell signalling assays (representing the NFκB, ATM/ATR, mTOR, AKT, and MAPK signalling pathways), and whole genome transcriptional profiling. In summary, Z-Cote and Nanosun, both uncoated but with primary particles of different sizes, clearly moderated the signalling of several cellular stress signalling pathways over a period of 4-6 h, induced a loss of cellular viability and antioxidant defences at 6 h and 24 h, and strongly perturbed the transcriptional profile at 24 h, with these effects showing little dependence on particle size. In contrast, treatment with the two coated nanoparticles, MAX and HP1, or ionic Zn from ZnSO4, had little to no effect on these cells.

nanomedicine-nanotechnology-Metabolic

Figure 1: Metabolic activity (measured by the MTS assay) in primary human liver stellate cells exposed to four types of ZnO nanoparticle or dissolved ZnSO4 at concentrations of 0-80 μg/mL. The signals at 24 h from cells, treated in quadruplicate for each concentration, were averaged and expressed as a percentage of signals from time‑matched untreated cells set as 100%. DMSO (5%) was used as a positive control for the assay.

The powders of ZnO nanoparticles used in these experiments have previously been characterised and described in detail [12], and their properties are summarised here in Table 1. Briefly, highlevel magnification by TEM showed the three powders provided by BASF (Z-COTE, HP1, and MAX) to consist of a heterogeneous mix of rectangular-shaped particles (Figure 2). Interestingly, given the uncoated Z-COTE is the base particle, the coated particles, HP1 and MAX, on average are both longer and narrower giving them larger aspect ratios (length/width) than Z-COTE [HP1, mean length 96 nm, mean width 28 nm, aspect ratio 3.4; MAX, length 95 nm, width 36 nm, aspect ratio 2.6; Z-COTE, length 73 nm, width 44 nm, aspect ratio 1.7]. Nanosun, supplied by Micronisers, comprised smaller, homogenous spherical particles with an average diameter of 25 nm. Consistent with their smaller size relative to the BASF particles, the specific surface area of the Nanosun particles (30 m2/g) was more than double those of Z-COTE, HP1 and MAX (13, 14.9, and 12 m2/g, respectively).

  Z-COTE   Namstm HP1 NIAX
  Coating None None Triethoxycaprylylsilane Dimethoxydiphenylsilane/triehoxycaprylyisilane crosspolymer
Powder# Batch raimber EHDA3001 4051 CNHE0602 FCHE1301
Primary particle size (TEM, nm, # measurements in parenthis Width: 44 ± 2 (260) Diameter: 25 ± 1 (238) Width: 28 ± 2 (201) Width: 36 ± 2 (300)
Length: 73 ± 4 (142) Length: 96 ± 6 (93) Length: 95 ± 5 (94)
Specific surface area (BET, m2/g) 13 ± 2 30 ± 3 14.9 ± 0.5 12 ± 1
In H20# Superoxide radical generation (% negative control) Dark: 800 ± 300* Dark: 280 ± 80 Dark: 220 ± 40 Dark: 170 ± 50
Light: 12,000 ± 3,000* Light: 2,000 ± 400 Light: 4,000 ± 700 Light: 4,000 ± 300
Endotoxin BD BD BD BD
Hydrodynamic particle size (DLS, nm, PDI in parenthesis) 0-time: 410 (0.3) 0-time: 600 (0.6) 0-time: 240 (0.3) 0-time: broad (1)
Zeta potential (mV) 0-time: 27 0-time: 9 0-time: -10 0-time: -7
Altered pH (relative to H2O only) 0-time: -0.1 0-time: 0.0 0-time: -0.1 0-time: -0.1
In complete cell culture medium (HM-5201) Hydrodynamic particle size (DLS, nm, PDI in parenthesis) 0-time: 260 (0.3) 0-time: 25 (0.7) 0-time: 300 (0.6) 0-time: 90 (0.2)
2h: 100 (0.8) 2h: 45 (0.8) 2h: 230 (0.4) 2h: 35 (0.7)
6h: 260 (0.3) 6h: 180 (0.2) 6h: 70 (0.7) 6h: 340 (0.4)
24h: 90 (0.2) 24h: 125 (0.3) 24h: 110 (0.2) 24h: 360 (0.5)
Particle size distribution (DCS, nm) 0-time: 90-500 0-time: ND 0-time: 20-900+ 0-time: 400-900+
Zeta potential (mV) 0-time: 8 0-time: 7 0-time: -10 0-time: -6
2h: -7 2h: -7 2h: -7 2h: -6
6h: -7 6h: -6 6h: -7 6h: -6
24h: -6 24h: -7 24h: -7 24h: -6
Altered pH over 24 h (relative to media only) 0-time: 0.0 0-time: 0.0 0-time: 0.0 0-time: 0.0
2h: 0.0 2h: 0.0 2h: 0.0 2h: -0.2
6h: 0.0 6h: 0.0 6h: 0.0 6h: +0.1
24h: -0.1 24h: 0.0 24h: 0.0 24h: 0.0

Table 1: Physical and chemical characteristics of the four types of ZnO nanoparticles.

nanomedicine-nanotechnology-morphologies

Figure 2: TEM images showing the morphologies of the ZnO nanoparticles used in this study. Scale bar for images A (Z-COTE), C (HP1), and D (MAX) are 200 nm. Scale bar for image B (Nanosun) is 500 nm.

DLS data are frequently reported and for this reason we include them here, but note that measurements by DLS are not suitable for this system, as evidenced by the large PDI values (Table 1). In water and in supplemented cell culture medium (HM-5201), all particles formed agglomerates with average hydrodynamic diameters greater than their primary particle size (Table 1). The particle size distribution in cell culture medium, as determined by DCS, was quite broad for all particle types, suggesting that a variety of agglomerate sizes were present following dispersion. Consequently, the cells were likely to have encountered a heterogeneous mix of suspended particles and agglomerates of particles in the culture treatments used here.

The surface charges for the uncoated Z-COTE and Nanosun changed from positive in H2O to negative in the supplemented cell culture medium (Table 1), suggesting that these particles became coated with negatively-charged components from the medium, most likely albumin which carries an overall negative charge at neutral pH [32]. The coated nanoparticles, HP1 and MAX, carried a negative surface charge in both H2O and cell culture medium. Medium pH did not substantially alter following 24 h cell-free incubation with any of the treatments, suggesting that cellular responses were not due to treatment-driven alterations in media pH.

All four types of particles generated higher levels of superoxide radicals in the light compared to the dark (Table 1), with Z-COTE generating the highest levels in both the light and dark (Table 1).

Results from the cell-signalling assays are presented in Figure 3 and Supplementary Table S1, and those from the cell functional assays are shown in Figure 4 and Supplementary Table S2. Due to the large number of genes whose activity was perturbed by treatment (Figure 5), transcript levels for individual genes are not listed, but a subset is discussed in the context of other results. The levels of phosphorylated c-JUN and, to a lesser extent, p38 proteins were clearly increased in cells treated with Z-COTE and Nanosun compared to untreated cells (Figure 3, Supplementary Table S1). The phosphorylation of these proteins, along with JNK, are key to mediating many cellular responses associated with a variety of stresses [33-35]. In particular, the phosphorylation and activation of these stress pathways has been demonstrated to initiate stress-induced cell death in many studies, through apoptotic or senescence mechanisms [33-36] (and here, for Z-COTE and Nanosun, is consistent with the spikes in caspase 3-7 activity at 6 h and 24 h and caspase-like proteinase activity at 6 h (Figure 4, Supplementary Table S2). However, substantial modulation of JNK phosphorylation was not observed (Figure 3). Subsequent treatment with a control agonist (TNFα) had no measurable effect (data not shown), indicating that JNK may have been expressed at concentrations below the detection level of the assay under the experimental conditions used here. Nevertheless, the strong activation of c-JUN and p38, combined with the statistically significant increase in the JUN, FOS, and GADD45 transcripts (data not shown), the last of which encodes a positive upstream regulator of p38 in response to cellular stress and growth arrest [37], provides a clear demonstration of the mobilization of hHSC stress responses when treated with uncoated ZnO nanoparticles. Treatment with these uncoated nanoparticles also elicited a negative influence on ERK and p70 phosphorylation, both of which were lower than control treatments after 2 h, although phosphorylation of pERK increased significantly at 12 h for all five treatments (Figure 3, Supplementary Table S1). Both of these proteins are involved in regulation of cell growth and survival [38,39], in addition to other cellular activities, and their de-phosphorylation here, in the context of the sharp decline at 24 h in metabolic activity in cells treated with either of the uncoated nanoparticles, but not HP1 or MAX (Figure 4, Supplementary Table S2), would be consistent with uncoated ZnO nanoparticle induction of a cell death, but a protective effect conferred by the addition of particle surface coatings.

nanomedicine-nanotechnology-signalling

Figure 3: Phosphorylation of selected key proteins from five major cell-signalling pathways in primary human liver stellate cells treated with ZnO nanoparticles or dissolved ZnSO4, at 30 μg/mL. Cells were treated in quadruplicate with ZnO nanoparticles, or ZnSO4, for each treatment time-point (0.5, 1, 2, 4, 6, 8, 12 and 24 h). Levels of protein phosphorylation were averaged, and expressed as the percentage of phosphorylation of the same protein in untreated cells set as 100%. The five cell-signalling pathways are: I NFκB (pro-inflammatory signaling); II ATM/ATR (DNA damage signalling); III mTOR (cell metabolism, growth, survival, and proliferation signalling); IV AKT (cell survival, proliferation, and apoptosis signalling); V MAPK (cell growth, survival, and differentiation signalling). The data used to generate this figure, and associated standard errors of means and statistical significance, are supplied separately in Supplementary Table 1.

nanomedicine-nanotechnology-nanoparticles

Figure 4: Changes in various cellular functions in primary human liver stellate cells treated with ZnO nanoparticles or dissolved ZnSO4, at 30 μg/mL. Cells were treated in triplicate or quadruplicate with ZnO nanoparticles, or ZnSO4, for each treatment time-point (6 and 24 h). Signal levels representing different cell functions were averaged, and are expressed here as the percentage of untreated cells set as 100%. Data used to generate this figure, and associated standard errors of means and statistical significance, are supplied separately in Supplementary Table 2.

Interestingly, the level of the AKT2 gene transcript (but not AKT1 or AKT3) was significantly higher (data not shown), and the phosphorylation of the AKT protein was strongly induced, in cells treated with Z-COTE or Nanosun, but not, or only weakly, by the coated nanoparticles (Figure 3). AKT phosphorylation regulates a number of pro-survival mechanisms within the cell through controlling anti-apoptotic mechanisms [40], and is typically considered to be a prosurvival response within the cell. AKT phosphorylation in cells treated with Z-COTE or Nanosun was accompanied by phosphorylation of the pro-apoptotic protein, BAD (Figure 3, Supplementary Table S1). Phosphorylation of BAD inhibits its pro-apoptotic activity by inhibiting the ability of BAD to form heterodimers with its anti-apoptotic binding partners BCL-2 and BCL-XL [39,41]. The early activation of the antiapoptotic AKT, and inhibition of the pro-apoptotic BAD in cells treated with the uncoated nanoparticles, alongside the stress-associated pathways, may reflect an initial activation of pro-survival mechanisms within the cells that were ultimately overwhelmed by pro-apoptotic mechanisms, as indicated by elevated caspase activity (Figure 4).

A number of signalling molecules examined here showed no, or only minimal, modulation of phosphorylation. The level of phosphorylated NFκB, a pro-inflammatory, pro-cell survival transcription factor [42], was increased by treatment with the control agonist, TNFα, as expected (data not shown), but were only slightly elevated and then depressed by the nanoparticle treatments over the time-course examined (Figure 3, Supplementary Table S1). The NFKB1 gene transcript, encoding NFκB, was either down-regulated (by Nanosun), or not significantly modulated (all other treatments) (data not shown). Further, the NFKB1B gene transcript, encoding an NFκB inhibitor, was up-regulated in cells treated with Z-COTE or Nanosun, but not by the other treatments (data not shown). Together, these data, supported by the relatively weak activation of NFκB in ZnO-nanoparticle-treated olfactory cells [12], suggest that cellular stress responses to uncoated ZnO nanoparticles may not be primarily mediated by NFκB.

Phosphorylation of Chk1 and Chk2 in cells treated with either of the coated ZnO nanoparticles was generally lower than, or not different from, untreated cells. However, phosphorylation of the Chk2 was elevated in cells treated with either of the uncoated particles, with peaks occurring at 6-8 hours with maxima of 176% in the presence of Z-COTE and 158% in the presence of Nanosun. The Chk1 pathway is typically mobilised when DNA replication is impeded, whereas Chk2 is activated in the presence of breaks in double-stranded DNA [43]. Therefore, the apparent activation of Chk2 over Chk1 by treatment with uncoated ZnO nanoparticles may indicate the presence of strand breakage in DNA rather than DNA adducts. Interestingly, statistically-significant perturbations in the levels of transcripts encoding proteins involved in the response to repair damaged DNA tended to be negative, and present only in cells treated with Z-COTE and Nanosun. Furthermore, TP53, encoding the tumour suppressor protein p53, was also down-regulated in cells treated with Z-COTE and Nanosun, but not HP1 or MAX (data not shown).

The protective effect of surface coatings against ZnO nanoparticleinduced cellular injury was also evident at the transcriptional level. A snapshot of the transcriptome was taken at 24 h post-treatment, and was indicative of strong disregulation induced by Z-COTE (10,037 perturbed genes) and Nanosun (9,784 genes), but far less so by HP1 (80 genes), MAX (33 genes), and ZnSO4 (145 genes) (Figure 5A). Differentially-expressed transcripts were plotted in VENN diagrams. Nanosun and Z-COTE showed by far the greatest disruption to the transcriptome, much of which was shared between the two treatments, but not by any of the other treatments (Figure 5B), further demonstrating the protective effect of the particle surface coatings.

nanomedicine-nanotechnology-transcript

Figure 5: Differential transcript activity in primary human liver stellate cells treated with ZnO nanoparticles or dissolved ZnSO4, at 30 μg/mL. Cells were treated in duplicate with ZnO nanoparticles or ZnSO4 for 24 h. A. Totals of differentially-expressed transcripts for each treatment grouped according to whether they were up-regulated (red) or down-regulated (green) compared to untreated cells. B. VENN diagrams showing whether differentially-expressed transcripts occurred uniquely within one treatment (unshared VENN), or were differentially activated by two or more of the treatments (intersecting VENN). For clarity, VENN treatments have been grouped into (i) uncoated ZnO nanoparticles or (ii) coated ZnO nanoparticles, both against an ionic zinc treatment (ZnSO4).

The comparative lack of a stress response in hHSCs treated with ZnSO4 is interesting given that the release of ionic zinc from ZnO nanoparticles has been postulated to drive their toxicity [6]. A consideration is that our use of a mass equivalent concentration of ZnSO4 to ZnO nanoparticles would result in a Zn2+ concentration from fully dissolved zinc sulphate that was 2.2x lower than that which would be produced from uncoated ZnO nanoparticles, assuming they were fully dissolved, which is unlikely. It is also important to note that ionic zinc is unlikely to be free, but rather will interact with components of the cell culture media. At concentrations greater than 5.5 μg/mL ZnO nanoparticles, the solubilised zinc fraction equilibrates at approximately 67 μM, more than 99% of which is complexed with organic ligands in the media (DMEM supplemented with 10% serum) [15]. Above this saturation limit, ZnO may produce zinc ions that may interact with cell culture components to precipitate as less-soluble zinc salts, such as zinc carbonate or zinc phosphate (depending on media components), or the ZnO nanoparticles may exist in a partially-dissolved form coated with zinc carbonate [15,44]. Therefore, at 30 μg/mL (the concentration used here), the concentration of free ionic zinc in cell culture medium treated with both ZnO nanoparticles and ZnSO4 was likely to have been similarly low. Further, the hHSCs were viable up to 277 μM zinc in ZnSO4-treated cells, assuming full dissolution (Figure 1). These results add support to the contention that the total amount of ionic zinc in cell culture medium may be of less importance to ZnO cytotoxicity than intracellular, localised high concentrations following the uptake and intracellular dissolution of ZnO or Zn-salt nanoparticles [15,44], although greater dissolution may be expected from ZnO than, for example, ZnCO3, based on their respective Ksp values in AQueous solution (3.9×10-10 and 1.4×10-11, respectively) [45].

The cellular stress and injury response observed here in response to treatment with uncoated ZnO nanoparticles is broadly consistent with those reported elsewhere using immortalised hepatic cell lines [9,23,24,26], as well as in the livers of mice and rats treated in vivo [17,18,20-22]. Interestingly, however, the presence of a surface coating identical to HP1, which was protective here, was shown to exert minimal protective effect in the liver C3A cell line [23]. Furthermore, the results for HP1 reported here contrast with the HP1 fingerprint in olfactory cells, which was similar to those of Z-COTE and Nanosun, albeit mitigated and time-delayed [12]. We suggested there that the stability and/or coverage of HP1’s coating may be inferior to that of MAX. While it is likely that the olfactory cells were simply more sensitive to ZnO nanoparticle treatment than the hepatic stellate cells used here, reflecting cell-line specific toxicity [46], we note that the experiments reported here were conducted prior to those in Osmond- McLeod by some months, raising the possibility that the trajectory of HP1 from benign to cytotoxic may reflect the impact of age and/or storage conditions on its surface coating, either by altering coat stability, or by elements adsorbed to the surface coating that may have altered the availability of HP1 to cells In vitro (unpublished data). A toxicological assessment of the impact of surface chemistry and toxicity of HP1 ZnO nanoparticles over time would be of interest to distinguish between the two possibilities, but was beyond the scope of this study.

In conclusion, the cellular profiles reported here showed that the surface-coated ZnO nanoparticles, HP1 and MAX, did not have a marked impact on cell signalling, function, viability, or transcriptional profile of human hepatic stellate cells. In contrast, the uncoated ZnO nanoparticles, Z-COTE and Nanosun, selectively activated pathways known to regulate cellular responses to abiotic stresses, and mechanisms known to control cell survival or apoptosis and senescence, reduce cell viability, and disregulate transcription. Further, these responses were generally not observed in cells exposed to ionic zinc alone. The presence of surface coatings, therefore, exerted a strongly protective effect against ZnO nanoparticle cytotoxicity. However, the influences of age and environmental exposure on the stability of surface coatings on particles, and on particle toxicity, over time should be investigated to further inform the use of surface coatings when applying a safetyby- design approach to the safe incorporation of ZnO nanoparticles in consumer products.

Acknowledgements

We thank: BASF and Micronisers Pty Ltd for their generous donations of the ZnO nanoparticles used in this study; Lynne Waddington, CSIRO, for size measurements by TEM; Gerry Triani, ANSTO, and Hong Yin, CSIRO, for surface area measurements; Victoria Coleman, National Measurement Institute of Australia, for DCS measurements; Ken Donaldson’s laboratory (Edinburgh University) for use of their EPR instrument and reagents; and Glenn Osmond-McLeod and Julie Osmond for assistance with analysis and visualisation of microarray datasets.

This work was funded by the CSIRO Animal, Food and Health Sciences, the CSIRO Advanced Materials Transformational Capabilities Platform, and the CSIRO Future Manufacturing Flagship.

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