Synthesis and Characterization of Cowry and Crab Shells Based Chitosan for Drug Delivery
Received Date: Jan 29, 2018 / Accepted Date: Feb 10, 2018 / Published Date: Feb 16, 2018
This work aims to synthesize chitosan from locally available cowry and crab shells for Pharmaceutical application in drug delivery. Chitosan was synthesized from both shells using standard deacetylation technique. The synthesized chitosan, piroxicam and lactose were employed in preparing the drug loaded tablets by direct compression technique and subjected to characterization with the aid of Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Spectroscopy (SEM) and optical microscopy. Finally, the drug release rate was investigated with in vitro drug dissolution test. The results of FTIR spectra confirmed that the biopolymer extract was chitosan and it also shows that there was no interaction between chitosan and the piroxicam. The morphological properties of the samples were found to be suitable for drug delivery. The applied load and composition of tablets influenced the drug release rates.
Keywords: Piroxicam; Cowry shell; Drug delivery; Crab shell; Chitosan
The National Institutes of Health Consensus Development Conference defined a biomaterial as ‘‘any substance (other than a drug) or combination of substances, synthetic or natural in origin, which can be used for any period of time, as a whole or as a part of a system which treats, augments, or replaces any tissue, organ, or function of the body’’ (10) while Williams defined a biomaterial as a nonviable material used in a medical device, intended to interact with biological systems (36). Some of the main areas of applications of biomaterials are classified into:
• Cardiovascular medical devices (stents, grafts and etc.)
• Orthopedic and dental applications (implants, tissue engineered scaffolds and etc.)
• Ophthalmologic applications (contact lenses, retinal prostheses and etc.)
• Bio-electrodes and biosensors.
• Burn dressings and skin substitutes.
• Drug delivery systems.
However, this work focuses on the application of biomaterial in drug delivery systems (DDS). Drug delivery systems introduced as formulations or instruments which enable to control the release rate of a biological agent (most especially a drug) in the target site. Drug delivery systems are an interface between patient and drug. Drugs can be introduced to the organ by various anatomical routes due to disease and drug type: Digestive system (oral, anal), oral, rectal, parenteral (subcutaneous, intramuscular, intravenous, arterial), mucous membranes, respiratory tract by inhalation, subcutaneous or intraosseous are man anatomical routes [1-12]. The various classifications of controlled released systems are indicated in Table 1 [13-24].
|Type of system||Rate-control mechanism|
|Diffusion control reservoir devices||Diffusion through membrane|
|Monolithic devices||Diffusion through bulk polymer|
|Water penetration controlled osmotic systems||Osmotic transport of water through semi permeable membrane|
|Swelling systems||Water penetration into glassy polymer|
|Chemically controlled monolithic systems||Either pure polymer erosion (surface erosion) or combination of erosion and diffusion (bulk erosion|
|Pendant chain systems||Combination of hydrolysis of pendant group and diffusion from bulk polymer|
|Regulated systems magnetic or ultrasound||External application of magnetic field or ultrasound to device.|
|Chemical||Use of competitive desorption or enzyme-substrate reactions. Rate control is built into device.|
Table 1: Classifications of controlled released systems.
Piroxicam is a non-steroidal anti-inflammatory drug (NSAID) used in treatment of different types of arthritis such as osteoarthritis, rheumatoid arthritis, ankylosing spondylitis and acute gout disease . It has a plasma half-life of approximately 50 hrs [25-30]. The chemical name for piroxicam is 4-hydroxy-2-methyl-N-2-pyridinyl- 2H-1,2 benzothiazine-3-carboxamide 1,1-dioxide. It is a member of the oxicam family, which though not carboxylic acids, are acidic by virtue of the enolic 4-hydroxy substituent (Figure 1). Piroxicam has poor solubility in water and when administered orally remains un-dissolve in the acidic environment such as obtained in the stomach causing bioavailability problems which may consequently lead to delay in the onset of drug effect [31-34]. In order to overcome these problems, it becomes necessary to develop a drug delivery system that will improve the solubility of piroxicam in the stomach.
Polymers are extensively used for the drug delivery. It has been established that they have the ability to form a matrix, or a membrane, that can control the release of drugs over a prolonged period, thus avoiding repetitive dosing. They have also been used to form (nano) carriers to deliver drugs with low solubility or biotechnology-based drugs [23,25]. Biodegradable polymers have found wide applications in drug delivery due to the ease of removal of the carrier after the release of the active pharmaceutical ingredients. To avoid side effects, especially when the carrier is injected, the polymer must be biocompatible. On this basis, natural polymers such as polysaccharides, polypeptides, or phospholipids are generally used as building blocks for drug formulations [8,20,31,35-38].
Chitosan is a nontoxic, biodegradable and biocompatible linear polysaccharide composed of randomly distributed ß-(1?4)-linked D-glucosamine (Figure 2) that has found diverse applications in drug delivery [23,27,28,32,33]. Over the last few decades, investigations had been focused on applications of chitosan and its fabricated products in drug delivery [2,13,35].
This study is therefore aimed at utilization of chitosan that will be synthesized from locally sourced crab and cowry shells, as a drug delivery vehicle that will enhance the bioavailability of piroxicam while simultaneously facilitating the controlled release of the drug [11,29].
Materials and Methods
The materials used were cowry shells and crab shells (sourced from Bode market, Challenge, Ibadan, South Western Nigeria), hydrochloric acid, sodium hydroxide, (BDH Chemicals Ltd Poole, England), acetone (Sigma-Aldrich Laborchemickalien GMBH, Seelze, Germany), piroxicam (Bond Chemicals, Awe, Nigeria), lactose, magnesium stearate, talc (Merck KGaA, Darmstadt, Germany) and di-potassium hydrogen orthophosphate (BDH Chemicals Ltd Poole, England).
The work entails the extraction of chitosan from cowry and crab shells, formulation of drug, and characterization of the synthesized chitosan and formulated tablet. This was with a view to ascertaining the suitability of the chitosan obtained from both shells for controlled drug delivery.
Chitosan synthesis: The cowry and crab shells were thoroughly washed to remove impurities such as sand, dirt and insect larva and subsequently oven-dried at 60°C. The washed and dried shells were then pulverized using a Rocklabs mill to pass through a 250 µm sieve. Deproteinization of the shell was carried out by weighing eighty grams of the pulverized shells into a conical flask and adding 0.1 M sodium hydroxide to it. The mixture was boiled and stirred at 100ºC for 2 h in a water bath to remove the protein. The product was decolorized by soaking in pure acetone for 24 h. It was then sieved with 300 µm sieve, washed to neutrality and dried in the oven at 100ºC for 2 h. This was followed by demineralization using 0.5 M hydrochloric acid to eliminate the calcium carbonate.
The residue, chitin, was scrapped into a Petri dish and dried in the oven at 100ºC for 2 h. The chitin was then deacetylated by boiling in 0.2 M sodium hydroxide, at 100ºC for 2 h to produce the chitosan. This was washed and tested with litmus paper to ensure a complete removal of the sodium hydroxide. Finally, the chitosan was oven dried at 90ºC for 24 h.
Preparation of piroxicam powder formulation: A 100 g batch of chitosan /piroxicam powder formulation was prepared using the different percentage compositions given in Table 1. Where piroxicam, the active drug ingredient, designated as P, was maintained at a constant dosage of 10 mg while the excipient, composed of the lactose (designated as L, which served as the filler) and the chitosan (designated as CCR and CCW for chitosan extracted from crab shell and cowry shell respectively) were varied in order to study the effects of their increasing weight percent on the physical, mechanical and dissolution properties of the formulated drug.
Each batch of 200 mg ± 2 mg piroxicam formulated powder was compressed for 30 seconds into tablets with predetermined loads ranging from 69.36 to 208.08 MPa on a Carver hydraulic press (Model C, Carver Inc. Wisconsin, USA), using an eight mm die and flat faced punch. The dies and punches were lubricated with a 2% w/v dispersion of magnesium stearate and talc in acetone prior to each compression . After ejection, the tablets were stored over silica gel for 24 h to allow for elastic recovery and prevent false low yield values .
Fourier Transform Infrared (FTIR) Spectroscopy: The FTIR spectra of chitosan obtained from crab and cowry shell (CCR and CCW) were recorded using an infrared spectrophotometer (Model IR Prestige 21, Shimadzu). All the Infrared spectra were recorded at resolution of 4 cm-1 over the frequency range from 4000-400 cm-1. Similarly the FTIR spectrum of the piroxicam loaded chitosan was studied to evaluate the interaction of the drug with the chitosan.
Analysis of particle shape and size: The morphology of the synthesized chitosan was studied using the scanning electron microscopy technique. Particle shape and size of samples from the synthesized materials and formulated drug were determined using optical microscopy. Optical images of the synthesized material were captured using an optical microscope (Leica Galen III Compound microscope with celestron digital microscope camera). The images were then analyzed using Image Pro Premier software (Media Cybernetics, Bethesda, MD, USA) in order to determine the particle descriptors of major and minor axis, length, perimeter and projected area. The shape factors, aspect ratio, roundness, irregularity and equivalent diameter were determined using equations (1-4).
(Where; b = Length of the minor axis, minimum Feret diameter, l = Length of the major diameter, maximum Feret diameter, A = Projected area of the particle and P = Perimeter)
Dissolution test for piroxicam tablet formulation: The dissolution rate of the piroxicam tablet was determined at 37 ± 0.5°C in 750 ml of 0.1 M hydrochloric acid using a Veego dissolution testing station and a stirring speed of 100 revolutions per minute. The tablet was placed in the rotating basket and 5 ml of the medium was sampled with a pipette after every 30 minutes. The same quantity of the medium was added at the same temperature immediately after each sampling to keep the volume of the dissolution medium constant. After 2 h of testing in 0.1 M of hydrochloric acid, the operation was completed by adding buffer and adjusting the pH within 5 minutes. With the apparatus in operation, 250 ml of 0.20 M solution of di-potassium hydrogen orthophosphate that has been equilibrated to 37 ± 0.5°C was added and it was adjusted with 31 ml of 2.0 M of sodium hydroxide to a pH of 6.8 ± 0.05. The operation was continued for 4 h. The concentration of dissolved piroxicam in the medium was determined spectrophotometrically using a Unicam 8620 UV/visible spectrophotometer (Pye Unicam, UK) at a wavelength of 360 nm. All determinations were made in triplicate and the results were given as average values.
Results and Discussion
Fourier Transform Infrared (FTIR) Spectroscopy
The FTIR spectra for biopolymer extracts and the formulated piroxicam powder are presented in Figure 3. These spectra confirmed that the structures of the two different chitosan are indeed chitosan. (6, 21) It is reported that the FTIR spectrum of chitosan exhibits major bands at 3444 cm-1, 2919 cm-1, 1659 cm-1, 1420 cm-1, 1080 cm-1 and 1033 cm-1 corresponding to OH group (3444 cm-1), stretching of CH2 (2919 cm-1), amide I band (1659 cm-1), vibration of OH ring (1420 cm-1), stretching of CH (1080 cm-1) and –C-O-C linkage (1033 cm-1). These bands, which are the main characteristic bands of chitosan [18,24], are similar to those observed in the spectrum of the chitosan synthesized from the crab and cowry shells, CCR and CCW (Figure 3), i.e., 3448.84 cm-1, 2931.90 cm-1, 1654.98 cm-1, 1429.3 cm-1, 1070.53 cm-1 and 1033.88 cm-1 Similarly, NH stretching bands are found in the spectrum of CCR and CCW at 3109.35 cm-1 and 3232.08 cm-1 which is similar to what was observed by Mohammed et al. . Consequently, it can be stated that the biopolymer extracts from crab and cowry shell are chitosan.
In addition, the absorption bands used to distinguish protein (1540 cm-1) and mineral (1798 and 876 cm-1) were not observed in the spectrum. The absence of both protein and mineral matters indicate that the materials may not elicit any cyto-toxic or adverse immunological response in the host when administered.
Furthermore, the spectrum of P-CCR and P-CCW, Figure 3b, displays absorption bands at 3448.84 cm-1, 3338.89 cm-1, 3103.57 cm-1, 2931.90 cm-1, 2521.05 cm-1, 1629.90 cm-1, 1577.82 cm-1, 1529.60 cm- 1, 1435.09 cm-1, 1352.14 cm-1, 1300.07 cm-1, 1215.19 cm-1, 1182.40 cm- 1, 1149.61 cm-1, 1120.68 cm-1, 1039.67 cm-1, 991.44 cm-1, 939.36 cm-1, 875.71 cm-1, 831.35 cm-1, 773.48 cm-1, 732.97 cm-1, 690.54 cm-1, 619.17 cm-1, 565.16 cm-1, 526.58 cm-1 and 457.14 cm.-1 It can be observed that the bands of chitosan were separated from that of piroxicam showing that there is no interaction between the chitosan obtained from crab shell (CCR) and the active pharmaceutical ingredient, piroxicam. A similar trend was observed in the spectrum of P-CCW, which displays absorption bands at 3853.90 cm-1, 3736.24 cm-1, 3446.91 cm-1, 3338.89 cm-1, 2931.90 cm-1, 2521.05 cm-1, 1791.93 cm-1, 1629.90 cm-1, 1525.74 cm-1, 1479.45 cm-1, 1435.09 cm-1, 1352.14 cm-1, 1300.07 cm-1, 1215.19 cm-1, 1182.40 cm-1, 1149.61 cm-1, 1039.67 cm-1, 939.36 cm-1, 860.28 cm-1, 831.35 cm-1, 773.48 cm-1, 732.97 cm-1, 626.89 cm-1, 565.16 cm-1, 526.58 cm-1, 457.14 cm-1 and 418.57 cm-1 indicating that there was no interaction between chitosan obtained from cowry shell (CCW) and piroxicam.
Dissolution properties of piroxicam tablets
The dissolution results, presented in the form of percentage drug released with time, are shown in Figures 4-6. Generally, polymer dissolution and erosion take place in three steps: Solvent penetration into the polymer matrix, polymer chain disentanglement and attainment of the threshold disentanglement. When solvent penetrates into the polymer matrix, it enhances the mobility of the polymer chains which eventually disentangle at the advancing front, separating the bond layer from the erosion/dissolution front. Chitosan disentangles as solvent passes through the pores, which provided an avenue for the drug release rate to increase. Thus, the synthesized chitosan was found to be an effective rate controlling polymer; by increasing the amount of chitosan (CCR/CCW) the drug release rate was observed to increase for the different formulations that were compressed using different compacting pressure as shown in Figures 4-6.
Effect of changes in compacting pressure and time on the drug released rate for the formulated tablets can be observed in Figures 4-6. Increase in the amount of drug released was observed with increasing time for all the formulation. However, the applied compacting pressure was also found to have influenced the drug release rate. The time taken for 50% of drug to be released for formulations manufactured using different compression forces indicated that drug release become slower with increasing applied force. This effect is particularly notice when comparing tablets manufactured using a very low compression force of 104.04 MPa with the tablets manufactured at higher compression forces of 138.72 MPa and 173.40 MPa. Depending on the compressibility behavior of the piroxicam powder, the porosity of the matrices was reduced with increasing compression force. This resulted in slower media uptake and media front movement into the matrix and consequently slow drug release rate.
Furthermore, the observed drug release rate was found to be higher in the formulations that had no lactose, the (10-90%) P-CCW and P-CCR formulation, compressed using different compressive forces. It can be noted that the piroxicam in the tablets were released before 6 hours. This may be due to the absence of lactose which serves as filler in the drug matrix. Lactose in the matrix results in lower porosity of the compacted tablet, hence lower drug release rate. Increasing concentration of lactose however resulted in improved osmotic pressure that may lead to accelerated water penetration into the matrix, resulting in a higher degree of polymer swelling and formation of more microcavities . Lactose, being water-soluble, produces more pores when in contact with water, forming more micro-cavities during the various mesophase formations. This causes the osmotic effect which promotes the erosion of chitosan  and subsequently the observed increase in the drug released from the matrices with increasing concentration of lactose. The lactose was therefore found to shortens the penetration time of the dissolution medium into the matrix and to also acts as a channeling agent by rapidly dissolving and easily diffusing outward, therefore increasing the matrix porosity with time. Consequently, the lactose was found to enhance the achievement of predetermined drug release profiles in the chitosan based controlled release matrices.
Microscopic and morphological properties of the samples
Optical microscopic imaging and quantitative image analyses have advanced the morphological characterization of powders, and the subsequent ability to use the knowledge acquired from such exercise to make important decisions during powder handling and the formulation of pharmaceutical dosage forms. Various morphological descriptors have been proposed and used by different image analysis software [1,5,7]. The results of the shape descriptors study carried out on the powdered samples of the synthesized materials, which include the aspect ratio, elongation, roundness, irregularity and equivalent circle diameter are presented in Table 2. From the result, it can be observed that all the samples have roundness value less than one. Roundness is a measure of how closely the projected area of the particle resembles a circle; with a perfect circle having a roundness value of 1. The higher the roundness value, the higher the percentage porosity, the better the flow rate. The CCR and CCW have higher roundness values (0.82 and 0.85 respectively) when compared to what was obtained for the different drug formulations. Consequently, they are less densely packed and have higher porosity. Correspondingly, P-CCW and P- CCR (10-90%) has the highest roundness value of 0.64 and 0.68 respectively in the drug formulation. Thus, they have higher porosity and higher rate of release of the active drug ingredient (Figures 4-6).
|P-CCRL/ P-CCWL (10-75-15)||P-CCRL/ P-CCWL (10-65-25)||P-CCRL/ P-CCWL (10-55-35)||P-CCRL/ P-CCWL (10-45-45)||P-CCRL/ P-CCWL (10-35-55)|
|Piroxicam (P %)||10||10||10||10||10||10|
Table 2: Chitosan piroxicam powder formulation.
Aspect ratio takes into consideration the maximum and minimum Feret’s diameter and these are utilized in the calculation of length and breadth of the particle (5, 1). It may be noted that the P-CCRL (10-35- 55%) has the lowest aspect ratio of 0.19. This may be responsible for its good packing properties, low percentage porosity and subsequently the observed low delivery of piroxicam, when compared to the other formulation. Similarly, irregularity measures the surface area compared to the size of the particle. A perfect circle, for example, has an irregularity of p [1,15,37,38]. In the powder formulation, it can be observed that P-CCW (10-90%) has the highest irregularity value of 2.94 while P-CCRL (10-35-55%) has the lowest is 2.29. These results further support the result of the aspect ratio and also the results obtained from SEM analysis (Figure 7) and proved that the powders were far from being perfect circles. These parameters may be responsible for the formulation having only CCR and CCW; which has the highest roundness value, highest irregularity and aspect ratio, hence closest to having circular configuration of all the samples. This resulted in it being less densely packed, consequently its high porosity, which led to the observed high drug release rate (Figures 4-6). In the same vein, it may be noted that P-CCRL (10-35-55%) that has the lowest aspect ratio of 2.29 is more densely packed, hence it has low porosity and consequently low drug release rate.
The particle size of the samples was determined using the equivalent circle diameter (ECD), which measures the diameter of a circle having the same cross sectional area as the powder. The higher is the ECD, the larger the mean particle size . Particle size had also been reported to affect the flow and packing properties of powder systems and their behavior during procedures involving bulk powder handling [16,19]. From Table 3, the extracted biopolymer, CCR and CCW, have lowest particle size, 9.07 and 9.00 respectively, this may be responsible for their high percentage porosity, hence the observed delivery rate (Figures 3-6). In the piroxicam formulations, P-CCRL (10-35-55%) has the highest particle size. The larger the particles size, the lower the percentage porosity of the formulated powder and the lower the rate of release of the active ingredient.
|Sample||Aspect ratio||Elongation||Roundness||Irregularity||Equivalent circle diameter (µm)|
Table 3: Morphological parameters of the biopolymer extracts and piroxicam powder samples.
The FTIR spectra indicated that the biopolymer extracts were chitosan and that there was no interaction between piroxicam and the biopolymer extracts. The inclusion of lactose in the piroxicam formulation resulted in tablets with low porosity because the lactose served as filler, which fills the pores of the piroxicam tablets during compression and thus enhanced the sustain release of piroxicam; however, the release rate has been found to also depend on time, compression pressure and the percentage of lactose and chitosan used in the formulation.
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Citation: Akinwole IE, Oyatogun GM, Adenigba AE, Ayodele TJ, Oluwasegun KM, et al. (2018) Synthesis and Characterization of Cowry and Crab Shells Based Chitosan for Drug Delivery. Bioceram Dev Appl 8: 107. DOI: 10.4172/2090-5025.1000107
Copyright: © 2018 Akinwole IE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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