alexa Synthesis and Cytotoxicity of Dinuclear Silver(I)-N-Heterocyclic Carbene Complexes | Open Access Journals
ISSN: 2161-1009
Biochemistry & Analytical Biochemistry
Like us on:
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Synthesis and Cytotoxicity of Dinuclear Silver(I)-N-Heterocyclic Carbene Complexes

Muhammad Adnan Iqbal1*, Rosenani A Haque1, Mohamed B Khadeer Ahamed2 and Abdul Majid AMS2

1The School of Chemical Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia

2EMAN Research and Testing Laboratory, The School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia

*Corresponding Author:
Muhammad Adnan Iqbal, PhD
School of Chemical Sciences
Universiti Sains Malaysia
Penang-11800, Malaysia
Tel: +60124384902
E-mail: [email protected]

Received Date: January 28, 2014; Accepted Date: February 28, 2014; Published Date: March 05, 2014

Citation: Iqbal MA, Haque RA, Ahamed MBK, Majid AMSA (2014) Synthesis and Cytotoxicity of Dinuclear Silver(I)-N-Heterocyclic Carbene Complexes. Biochem Anal Biochem 3: 147. doi:10.4172/2161-1009.1000147

Copyright: © 2014 Iqbal MA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Biochemistry & Analytical Biochemistry

Abstract

Synthesis of two new para-xylyl linked bis-benzimidazolium salts (III-IV) and respective dinuclear Ag(I)-N-heterocyclic carbene complexes (V-VI) has been described. The compounds were tested against human colorectal cancer cells (HCT 116) which showed a dose-dependent cytotoxic activity. The IC50 values were found in the range 0.03–65.9 μM. 5-Fluorouracil was used as standard drug (IC50=5.9 μM).

Keywords

Benzimidazole; Bis-benzimidazolium salts; N-heterocyclic carbenes (NHCs); Ag(I)-NHC Complexes; human colon cancer (HCT 116).

Introduction

Human colon canceris the third most common and the fourth most frequent cause of cancer deaths worldwide [1]. The marketing chemotherapeutic drugs for the treatment of colon related cancer are Carboplatin, Levamisole, Miltefosine, Semustine and Floxuridine (Chart 1). The chart displays that most of the drugs are organic compounds. Among these, only Carboplatin is a metal based anticancer drug that has been recently approved to treat human colon cancer.

biochemistry-analytical-biochemistry-anticancer

Chart 1: Marketing anticancer drugs for the treatment of colorectal cancer.

The incorporation of Carboplatin against cancer has recently attracted researchers to further explore the area of metal based anticancer drugs [2,3]. Except transition metals, the organometallic compounds based on coinage metals (Ag, Au, Cu) have now attracted the researchers for medicinal applications [4,5]. Recently, Au(I)-NHC complexes have been tested against HCT 116 cell line (Chart 2), showed significant in vitro anticancer results [6]. However, according to the published studies, Ag(I)-N-Heterocyclic Carbene (NHC) complexes have been rarely studied against this specific cancer. The current study is an effort in this area.

biochemistry-analytical-biochemistry-complexes

Chart 2: The Au(I)-NHC complexes tested against human colon cancer cell line (HCT 116) and their reported IC50 (μM) values.

Silver(I)-NHC complexes have shown excellent anticancer results against various types of cancerous cell lines [7-23]. Silver containing organometallic compounds, other than silver(I)-NHC complexes, have also shown significant biological activities [23,24]. Due to aforementioned reasons, two new bis-benzimidazolium salts (organic salts, III-IV) were synthesized and bonded with silver metal ions through carbene carbon (metal complexes, V-VI) to further assess their medicinal efficacy against HCT 116. This work is a consistency of our slogs [14,15,24,25].

Experimental

Reagents and instruments: NMR spectra were recorded on Bruker 500 MHz Ultrashield™ spectrometer at ambient temperature. FT-IR spectra were recorded on Perkin Elmer-2000. Elemental analyses were carried out on a Perkin Elmer series II, 2400 microanalyzer. The melting points were assessed by using a Stuart Scientific SMP-1 (UK) instrument.

RPMI 1640 was purchased from ScienCell, USA. Trypsin and heat inactivated foetal bovine serum (HIFBS) were obtained from GIBCO, UK. Phosphate buffered saline (PBS), penicillin/streptomycin (PS) solution, MTT reagent and 5-fluorouracil were purchased from Sigma-Aldrich, Germany. All other chemicals used in this study were analytical grade or better.

Human colorectal tumor (HCT 116) cell line was purchased from American type culture collection (Rockvill, MD, USA). HCT 116 cell line has been derived from colonic epithelial carcinoma. The cells were maintained in RPMI 1640 containing 10% HIFBS and 1% PS. Cells were cultured in 5% CO2-humidifed atmosphere at 37°C. Preparation of cell culture and MTT Assay was performed according to the reported procedure [15,24].

Synthesis: Syntheses of N-substituted benzimidazoles (III), bis-benzimidazolium salts (III-IV) and respective Ag(I)-NHC complexes (V-VI) were carried out according to the our previously reported procedure [9,13-15,24] .

3,3'-(1,4-phenylenebis(methylene))bis(1-pentyl-benzimidazolium) dibromide (III.2Br).

Following the reported procedure [15,24], N-pentylbenzimidazole (I), 20 mM (3.76 g) and 1,4-bis(bromomethylene)benzene 10 mM (2.63 g). The product appeared as white precipitates, filtered, washed with fresh 1,4-dioxane (3×5 ml) and dried at ambient temperature. Beige colored powder; yield: 88% (5.62 g); mp 204-206°C. FT-IR (KBr, vmax, cm-1): 3136, 3062, 3036 (C-Harom); 2946, 2932, 2862 (C-Haliph); 1625, 1570 (C=C stretch); 1352, 1385, 1421, 1452, 1476 (Carom-Nbenzimi). 1H NMR (500 MHz, DMSO-d6): δ=0.83 (t, J=7.0 Hz, 6H, 2×CH3), 1.28 (br.m, 8H, 4×CH2), 1.91 (qnt, 4H, 2×CH2), 4.46 (t, J=7.0 Hz, 4H, 2 ×N-CH2-R), 5.76 (s, 4H, 2×N-CH2-Ar), 7.56 (s, 4H, Ar-H), 7.62 (sext, 4H, Ar-H), 7.93 (d, J=8.5 Hz, 2H, Ar-H), 8.04 (d, J=8.0 Hz, 4H, Ar-H), 10.03 (s, 2H, 2×NCHN). 13C NMR (125.7 MHz, DMSO-d6): δ=13.6 (CH3), 21.7, 25.2, 28.2, 30.3, 39.2 (CH2), 46.4 (N-CH2-R) 49.2 (N-CH2- Ar), 113.8, 126.6, 128.8, 130.8, 131.3, 134.5 (Ar-C), 142.4 (NCHN). Anal. Calcd for C32H40Br2N4: C, 60.01; H, 6.29; N, 8.75. Found: C, 61.01; H, 6.32; N, 8.31. (qnt=quintet; br.m=broad multiplet).

3,3'-(1,4-phenylenebis(methylene))bis(1-heptyl-benzimidazolium) dibromide (IV.2Br).

Following the reported procedure [15,24], N-heptylbenzimidazole (II), 10 mM (2.16 g) and 1,4-bis (bromomethylene) benzene 5.0 mM (0.88 g). A sticky white, soap like formation occurred, filtered, washed with fresh dioxane (3×5 ml), and converted to its counter ion part by metathesis reaction in H2O/MeOH system (1:1). White powder; yield: 58% (2.40 g); mp 202-204°C. FT-IR (KBr, vmax, cm-1): 3166, 3108 (C-Harom); 2952, 2930, 2854 (C-Haliph); 1620, 1584 (C=C stretch); 1489, 1445, 1374, 1353 (Carom-Nbenzimi). 1H NMR (500 MHz, DMSO-d6): δ=0.82 (t, J=7.0 Hz, 6H, 2×CH3), 1.24 (br.d, 16H, 8×CH2), 1.92 (qnt, 4H, 2×CH2), 4.48 (t, J=7.2 Hz, 4H, 2×N-CH2-R), 5.72 (s, 4H, 2×N-CH2- Ar), 7.52 (s, 4H, Ar-H), 7.64 (br.m, 4H, Ar-H), 7.86 (d, J=8.0 Hz, 2H, Ar-H), 8.05 (d, J=8.0 Hz, 2H, Ar-H), 9.85 (s, 2H, 2×NCHN). 13C NMR (125.7 MHz, DMSO-d6): δ=13.7 (CH3), 21.8, 25.6, 27.8, 28.2 (alkyl chain 5×CH2), 46.8 (N-CH2-R), 49.7 (N-CH2-Ar), 113.7, 126.7, 128.5, 130.6, 131.3, 135.4 (Ar-C), 142.2 (NCHN). Anal. Calcd for C36H48F12N4P2: C, 52.30; H, 5.85; N, 6.78. Found: C, 52.21; H, 5.72; N, 6.55.

1,4-bis(N-pentylbenzimidazol-1ylmethyl)benzene-silver(I) bis(hexaflourophosphate) complex (V)

Following the reported procedure [15,24], III.2Br, 3.12 mM (2.0 g) and silver oxide, 4.7 mM (1.0 g). beige colored powder; yield: 82% (1.88 g); mp 308–310°C. FT-IR (KBr, vmax, cm-1): 3064 (C-Harom); 2955, 2932, 2873 (C-Haliph); 1346, 1392, 1443 (Ag-Carom-Nbenzimi) .1H NMR (500 MHz, Acetonitrile-d3): δ=0.85 (t, J=7.5 Hz, 12H, 4×CH3), 1.32 (sext, 12H, 6×CH2), 1.83 (qnt, 8H, 4×CH2), 4.48 (t, J=7.0 Hz, 8H, 4×N-CH2-R), 5.57 (s, 8H, 4×N-CH2-Ar), 7.13 (s, 8H, Ar-H), 7.35 (br.m, 8H, Ar-H), 7.50 (d, J=8.0 Hz, 4H, Ar-H), 7.68 (d, J=8.5 Hz, 4H, Ar-H). 13C NMR (125.7 MHz, Acetonitrile-d3): δ=14.0 (CH3), 20.8, 30.9, 33.1, (alkyl chain 3×CH2), 50.1 (N-CH2-R), 52.7 (N-CH2-Ar), 113.7, 125.4, 128.5, 134.2, 137.2 (Ar-C), Ag-C was not observed. Anal. Calcd for C64H80Ag2N8P2: C, 52.40; H, 5.50; N, 7.64. Found: C, 52.35; H, 5.46; N, 7.61.

1,4-bis(N-heptylbenzimidazol-1ylmethyl)benzene-silver(I) bis(hexaflourophosphate) complex (VI)

Following the reported procedure [15,24], IV.2Br, 4.0 mM (2.8 g) and silver oxide, 7.0 mM (1.6 g). beige colored powder; yield: 71 % (2.2 g); mp 283-285°C. FT-IR (KBr, vmax, cm-1): 3108, 3056, 3031 (C-Harom); 2951, 2921, 2852 (C-Haliph); 1341, 1412, 1434, 1476 (Ag-Carom-Nbenzimi). 1H NMR (500 MHz, DMSO-d6): δ=0.87 (t, J=7.0 Hz, 12H, 4×CH3), 1.15 (br.m, 16H, 8×CH2), 1.25 (qnt, 8H, 4×CH2), 1.35 (qnt, 8H, 4×CH2),1.91 (qnt, 8H, 4×CH2), 4.61 (t, J=6.5 Hz, 8H, 4×N-CH2-R), 5.71 (s, 8H, 4×N-CH2-Ar), 7.20 (s, 8H, Ar-H), 7.41 (sext, 8H, Ar-H), 7.74 (d, J=8.0 Hz, 4H, Ar-H), 7.86 (d, J=8.0 Hz, 4H, Ar-H). 13C NMR (125.7 MHz, DMSO-d6): δ=13.5 (CH3), 21.7, 26.1, 28.5, 30.0, 31.5 (alkyl chain 5×CH2), 48.5 (N-CH2-R), 50.7 (N-CH2-Ar), 112.1, 124.2, 127.2, 133.5, 136.3 (Ar-C), Ag-C was not observed. Anal. Calcd for C80H112Ag2N8P2: C, 59.84; H, 6.42; N, 7.75. Found: C, 58.57; H, 6.53; N, 7.81.

Results and Discussion

Synthesis: The reaction of two equivalents of N-alkylatedbenzimidazole with 1,4-bis(bromomethylene)benzene in 1,4-dioxane at 100°C for 24 h afforded the para-xylyl linked bis-benzimidazolium salts (III-IV) with good yields. The salts (III-IV) appeared as white precipitates in 1,4-dioxane, filtered and washed with fresh 1,4-dioxane (3×5 ml). The precipitates so obtained were dried at ambient temperature for 24 h. This is in accordance with the previously reported para-xylyl linked bis-benzimidazolium salts having different terminal N-substitutions [14,15]. The salts so obtained were found to be soluble in polar solvents likemethanol, ethanol, DMSO, DMF but remained insoluble in non-polar solvents like diethyl ether, dichloromethane and chloroform. Also, the reported salts were found insoluble in water. However, on changing counter ions from halide to hexaflourophosphate (PF6), the solubility of salts affected. For example, with PF6 as counter anions the salts became insoluble in methanol, ethanol but soluble in acetonitrile. However, the salts remained soluble in DMSO and DMF with both types of anions. The change of counter anions changes the polarity of compound. With the halide as counter anion polarity of these salts increased.

Synthesis of dinuclear Ag(I)-NHC (V-VI) complexes using bisbenzimidazolium halides (III-IV) as starting material is of significance importance, because using this method pure form of desired complexes can be obtained with ease. Scheme 1 shows three simple steps for the synthesis, starting from N-alkylated benzimidazoles to PF6 salts of dinuclear Ag(I)-NHC complexes. As a preliminary confirmation of successful synthesis, it was notice that the melting points of Ag(I)-NHC complexes were significantly higher than compared to respective ligands. This is in accordance with the previous the reports [14,15,26,27].

biochemistry-analytical-biochemistry-benzimidazoles

Scheme 1: Synthesis of N-alkyl benzimidazoles (I-II), 3,3'-(1,4-phenylenebis(methylene))bis(1-alkyl-Benzimidazolium) salts (III-IV), and Ag-NHC complexes (V-VI). complexes.

FT-IR spectra of the compounds: The synthesized bisbenzimidazolium salts do not have many functional groups to be distinguished by FT-IR spectroscopic technique but it is possible to compare the spectral features of ligands (III-IV) and respective Ag(I)- NHC (IV-V) complexes. Some noticeable changes in specific regions could be observed as primary indicators of a successful synthesis [15].

The modes of the Csp3-H stretching vibrational bands, both in alkyl benzimidazoles (I-II) and bis-benzimidazolium salts (III-IV), appeared at around 2850 to 3000 cm-1 (C-Haliph). This range of vibrations is due to the presence of C-H (sp3-s) stretching of alkyl chains and methylene (N-CH2-Ar) groups. This can be further classified as the vibrational bands in the range 2840–2855 cm-1 appeared for CH3 stretchings, 2920– 2925 cm-1 for CH2 anti-symmetric stretchings, and 2955-2960 cm-1 for CH2 symmetric stretchings. A vivid and intense band was observed in the range 1350 to 1500 cm-1 assigned to the stretching vibrations of benzimidazole ring due to the presence of -HC=N- module [15,28]. The ring vibrations, other than –HC=N, are intense bands at around 1050 and 1220 cm-1.

Some interesting FT-IR features to confirm a successful synthesis of Ag(I)-NHC complexes were observed [15]. It was observed that bonding of NHC carbon with silver ion strengthened vibrations in the range 1350 to 1500 cm-1 and a typical “four fingers (f.fs)” pattern appeared for the synthesized Ag(I)-NHC complexes [13,14,24,29]. This region is specific for –C=N (Carom-Nbenzimi) stretching and CH2 bending vibrations. The contemplated “f.fs” patterns were entirely different than all the respective vibrations in azolium salts and were easily discernible. This information also remained a primary confirmation for the successful syntheses of desired Ag(I)-NHC complexes.

FT-NMR spectra of the compounds: FT-NMR spectra of all the compounds were scrutinized either in DMSO-d6 or acetonitrile-d3 over the scan range 0 to 11 δ ppm for 1H NMR and 0 to 200 δ ppm for 13C NMR studies. 1H NMR spectra of the salts (III-IV) evidenced a sharp singlet around 10 ± 0.3 δ ppm assigned to the benzimidazolium ring (NCHN) acidic proton. These signals are in accordance with previous reports [30,31]. Synthesis of Ag(I)-NHC complexes was indicated by the recede from view of this acidic proton peak and notice of observable changes in aromatic (Ar-H) peaks (Supplementary I). The methylene (N-CH2-Ar) group, which connects para-xylyl unit with benzimidazolium units, exhibited a sharp singlet at 5.7 δ ppm. Furthermore, the resonance of N-substituted alkyl chain protons appeared in the range 0.8-4.5 δ ppm. Similarly, the structural features of the salts were confirmed by the 13C NMR data. The spectra of all the salts displayed a distinguished peak in the most down field region at 142 δ ppm assigned to the benzimidazole ring carbon (NCHN). The signals for methylene (N-CH2-Ar) carbon appeared at 49.2 δ ppm and for alkyl chain in the range 13.7–46.8 δ ppm [15,24].

After complexation with Ag ions, usually two doublets appear at about 188 δ ppm with Ag-C coupling constants ca. 206 ± 2 Hz and 184 ± 2 Hz [9,14,25,32]. These doubletes have been mostly observed in dimeric complexes of structure [L2Ag2]2+ due to carbene carbon bonding to C-Ag107 and C-Ag109, respectively [15,33]. In some cases, these doublets do not appear. In the current research, this happened for both complexes (see 13C NMR of VI, Supplementary II). This light be due to bulky N-alkyl substitutions where the NMR signals for Ag-C coupling could not be generated for the analyzed quantity. However, resonances of aromatic carbons, in ligands (III-IV) ranging ≈113- 135 δ ppm were observed in the range ≈112–137 δ ppm in respective dinuclear complexes (V-VI) with noticeable splitting. Also, the C-2 carbon (NCN) disappeared in respective complexes indicating its possible downfield movement. Furthermore, the resonances due to N-C-Ar carbon and alkyl chain carbons were observed in the chemical shift regions 51 and 13-49 δ ppm, respectively. These signals are 2-3 δ ppm downfield compared to respective ligands. All these observations indicated the formation of desired complexes (IV-VI).

In vitro anticancer activity: Effect of benzimidazolium salts (ligands) and respective silver(I)-NHC complexes on proliferation of HCT 116.

In this work, antiproliferative potential of NHC pro-ligands and respective dinuclear Ag(I)-NHC complexes was evaluated against HCT 116 cancerous cells. The results have been compiled as mean percentage inhibition of cell proliferation (±S.D). The compounds (III-VI) were tested against HCT 116, exhibited a strong antiproliferative activity with IC50 values in the range 0.03–65.9 μg/ml (Table 1). The results were more pronounced than the standard drug 5-fluorouracil (IC50=5.9 μM). Recently, we reported anticancer activity of meta-& para-xylyl linked bis-benzimidazolium salts and respective dinuclear Ag(I)- NHC complexes. We found that in general the complexes are more active against human colon cancer compared to respective ligands. The current results support our previous findings. Furthermore, Benzimidazole, the starting material, was also tested against HCT 116 cell lines and was found almost inactive (IC50=>200 μM). Hence the bis-benzimidazolium salts (IC50=0.3–65.9 μM) were found to be active against human colon cancer compared to benzimidazole moiety whereas the Ag(I)-NHC complexes of these bis-benzimidazolium salts were further found to be relatively active (IC50=0.03–5.6 μM).

  Compounds HCT 116
Ligands III 65.9
IV 0.3
Ag(I)-NHC Complexes V 5.6
VI 0.03
Starting Material benzimidazole > 200
StandardDrug 5-Fluorouracil 5.9

Table 1: IC50 values of tested samples against HCT 116 cancer cell line.

The Figure 1 illustrates the anti-proliferative effect of the test compounds. The graphs clearly show that, all the compounds inhibited the proliferation of HCT 116 cells in a dose dependent manner. Figure 2 shows the cell images of cell line with 48 hours control without and with drugs.

biochemistry-analytical-biochemistry-proliferative

Figure 1: Dose dependent anti-proliferative effect of synthetic ligands and their metal complexes on human colorectal tumor cells (HCT 116).

biochemistry-analytical-biochemistry-treatment

Figure 2: HCT 116 cell images were captures under an inverted phasecontrast microscope at × 200 magnification with a digital camera at 48 hours after treatment with the test samples.

A) Cancer cells from the control group exhibited a thoroughly confluent growth with densely proliferating HCT 116 cells. B) Treatment with the ligand IV exhibited noteworthy inhibitory effect on cancer cell proliferation with IC50=0.3 μM. The picture divulged the affected cellular morphology of the treated cells. The intercellular junctions were disconnected and cells appeared less intacted. C) Photomicrograph of HCT 116 cells injected with the complex VI showed a strongest cytotoxic effect of the complex (IC50=0.03 μM). It can be visualized clearly that the compound affected the proliferation of all most all the cells of the group which rendered the cells to lose their pseudopodial like extensions. D) Treatment with the ligand III showed a moderate cytotoxicity(IC50=65.9 μM) than compared to the other compounds tested. E) HCT 116 cells treated with the complex V demonstrated a significant cytotoxicity (IC50=5.6 μM) as the population of cells decreased substantially within the 48 hours of treatment. F) Cells treated with the Standard drug 5-fluorouracil (IC50=5.9 μM) showed decreased workability.

Conclusion

In conclusion, two new para-xylyl linked bis-benzimidazolium salts and their Ag(I)-NHC complexes were synthesized. The compounds were characterized tested for their possible cytotoxicity on human colorectal cancer (HCT 116). All tested compounds showed dosedependent cytotoxicity against human colon cancer. The IC50 values for HCT 116 cells were ranged between 0.03 and 65.9 μM. Some of the tested compounds showed IC50 values significantly better than commercially available drug (5-FU, IC50=5.9 μM). Among all, the complex VI was found to be the most active against human cancer cell line (IC50=0.03 μM). Based on the results it can be concluded that incorporation of silver ions with organic moieties enhances their anticancer potential.

Acknowledgements

RAH thanks Universiti Sains Malaysia (USM) for the short term grant (1001/ PKIMIA/823082). MAI is grateful to (IPS) USM for financial support [fellowship: USM.IPS/JWT/1/19 (JLD 6): P-KD0030/11(R)] and funds [P-KM0018/10(R)-308/ AIPS/415401] for a research attachment to University of Western Australia (UWA), Perth, Australia.

References

Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Relevant Topics

Article Usage

  • Total views: 11835
  • [From(publication date):
    April-2014 - Oct 23, 2017]
  • Breakdown by view type
  • HTML page views : 8018
  • PDF downloads :3817
 

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords